H

155.16 His Periodic Chart of Amino Acids D

133.10 Asp
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137.14 115.09
C6H9N3O2 C4H7NO4

Histidine Aspartic Acid

R
174.20
156.19
Arg F
165.19
147.18
Phe A
89.09
71.08
Ala C
121.16
103.14
Cys G
75.07
57.05
Gly Q
146.15
128.13
Gln E
147.13
129.11
Glu
C6H14N4O2 C9H11NO2 C3H7NO2 C3H7NO2S C2H5NO2 C5H10N2O3 C5H9NO4

Arginine Phenylalanine Alanine Cysteine Glycine Glutamine Glutamic Acid

K
146.19
128.17
Lys L
131.18
113.16
Leu M
149.21
131.20
Met N
132.12
114.10
Asn S
105.09
87.08
Ser Y
181.19
163.17
Tyr T
119.12
101.10
Thr
C6H14N2O2 C6H13NO2 C5H11NO2S C4H8N2O3 C3H7NO3 C9H11NO3 C4H9NO3

Lysine Leucine Methionine Asparagine Serine Tyrosine Threonine

I
131.18
113.16
Ile W
204.23
186.21
Trp P
115.13
97.12
Pro V
117.15
99.13
Val Basic

Non-polar
(hydrophobic)
1-Letter Amino
Acid Code
S
105.09
Ser 3-Letter Amino
Acid Code

C6H13NO2 C11H12N2O2 C5H9NO2 C5H11NO2 Relative Molecular 87.08

Polar, uncharged Mass C3H7NO3
Acidic M r – H20
Chemical
Molecular Formula Structure

Serine Chemical
Isoleucine Tryptophan Proline Valine Name

Common Fmoc-Strategy SPPS* Protecting Groups Absorption and Emission Characteristics of Chromophores and Fluorophores Common Boc-Strategy SPPS* Protecting Groups

Excitation Emission
Fluorophore Wavelength Wavelength
Boc
Fmoc Abz 320 nm 420 nm t-Butyloxycarbonyl
9-Fluorenylmethyloxy- (2-Aminobenzoyl or Anthraniloyl) M r = 101.13
carbonyl N-Me-Abz 340 - 360 nm 440 - 450 nm
M r = 223.25 (N-Methyl-anthraniloyl)
AFC 395 - 400 nm 495 - 505 nm
(7-Amido-4-trifluoromethylcoumarin) Tos
AMC 360 - 380 nm 440 - 460 nm Tosyl
Mtt (7-Amido-4-methylcoumarin) M r = 155.20
4-Methyltrityl Dansyl 342 nm 562 nm
M r = 257.36
(5-(Dimethylamino)naphthalene-1-sulfonyl)
EDANS 340 nm 490 nm
(5-[(2-Aminoethyl)amino] naphthalene-1- Mbzl
sulfonic acid) 4-Methylbenzyl
FITC 490 nm 520 nm M r = 105.16
Pmc (Fluorescein isothiocyanate)
2,2,5,7,8-Pentamethyl-
Mca 325 nm 392 nm
chroman-6-sulfonyl
((7-Methoxycoumarin-4-yl)acetyl)
M r = 267.37
4MβNA 335 - 350 nm 410 - 440 nm Bom
(4-Methoxy-β-naphthylamide) Benzyloxymethyl
βNA 320 - 340 nm 410 - 420 nm M r = 121.16
EP 0 293 073 B1
(β-Naphthylamide)
US Patent 4,946,971
owned by Bachem Trp 280 nm 360 nm
(Tryptophan) 2-Chloro-Z
2-Chlorobenzyloxy-
carbonyl
M r = 169.59
Extinction Molar Extinction

tBu
Chromophore Wavelength Coefficient

t-Butyl pNA 405 nm ε405 nm = 9450 M-1cm-1 For

M r = 57.12 (p-Nitroanilide) 410 nm ε410 nm = 8800 M-1cm-1 Formyl
Values listed are as reported in the literature *SPPS = Solid Phase Peptide Synthesis M r = 29.02

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