Professional Documents
Culture Documents
Artium Baccalaureus (3rd Year): Ecology and Evolutionary Biology, certificate in Sept./15 -
Spanish and Environmental Sciences. Present
Princeton University, New Jersey, United States of America.
Princeton Environmental Institute Scholarship: Field & Research Intern with the June/1-Aug./17
Putnam Lab
Bermuda Institute of Ocean Sciences (BIOS), St. George’s, Bermuda.
o Conducted research under Dr. Samantha de Putron, Dr. Hollie Putnam and Ph.D. candidate Kevin Wong
to study the reproductive plasticity of adult Porites astreoides colonies in various environmental
conditions experienced at an inshore patch reef.
o Measured calcification rates, RGB color changes, respiration and photosynthesis rates at different time
points.
o Collected and preserved lipid samples, DNA samples and microbial samples.
o Achieved a high laboratory and data processing productivity while assisting with other collaborative
projects occurring at BIOS.
Coral Reef Ecology Course: Teacher Assistant Aug./17
Bermuda Institute of Ocean Sciences (BIOS), St. George’s, Bermuda.
o Assist students with multiple line and quadrat surveys that asses and identify benthic
cover, fish species size and abundance, size and abundance of juvenile recruits and
presence of coral disease.
o Prepared solutions and lab materials; photocopy/print course materials; set up AV
equipment; assist with any other duties as assigned by the course instructors.
o Assist students in all laboratory equipment use and methodology.
o Grade all laboratory reports, experiments, exams and presentations.
Princeton Environmental Institute Scholarship: Field & Research Intern with the May/6-Sep/16
Sigman Lab.
Bermuda Institute of Ocean Sciences (BIOS), St. George’s, Bermuda.
o Conducted research under Dr. Samantha de Putron, Dr. Daniel Sigman and Ph.D. candidate Victoria
Luu on the Effect of feeding and light on the growth of juvenile colonies of the Atlantic coral Porites
astreoides.
o Fed juvenile Porites astreoides.
o Measured growth of juvenile Porites astreoides at multiple time points.
o Measured calcifications rates at multiple time points.
o Collected and preserved lipid samples, DNA samples and microbial samples.
o Collected nutrient, water and ammonia samples daily to monitor conditions.
Sigman Lab Laboratory & Research Assistant Sep/16-Jan/17
Sigman Lab, Princeton University, New Jersey, United States.
o Processed samples collected the previous summer to conclude our research. This
included performing mass.
Freshman Scholar’s Institute June/15-Aug/15
Schultz Lab, Princeton University, New Jersey, United States of America.
o Achieved multiple site-directed mutagenesis of transformed bacterial plasmid,
which provided the research team with the means to study the role of certain genes
in C. elegans.
o Performed restriction digest and DNA sequence analysis of recombinant plasmid
constructs, which confirmed the identity of recombinant plasmids and presence
mutated sites.
o Successfully grew transformed bacteria cultures, which provided the research team
with cultures for DNA mutation in the nematode C. elegans.
POSTER PRESENTATIONS
December 13-15, 2017. European Coral Reef Symposium (ECRS), Oxford, England. Victoria Luu, Samantha J
de Putron, Valeria Schmidt*, Anne Cohen, Daniel Sigman: Effect of feeding and light on the growth of juvenile
colonies of the Atlantic coral Porites astreoides.
FIELD EXPERIENCE
R.V. Atlantic Explorer – Sargasso Sea July/16– BSAE1712
Sigman/de Putron Lab – St. George’s, Bermuda June/16 – Sept/16
Putnam/de Putron Lab – St. George’s, Bermuda May/17 – Sept/17
SKILLS & TECHNIQUES
Laboratory
o Symbiodinium quantification o PCR amplification & o Plasmid isolation
o Coral growth monitoring optimization o Microorganism culture
o Larval analysis (fecundity, o Agarose gel o Site-directed mutagenesis
lunar periodicity, volume, electrophoresis o Plasmid transformation
settlement and growth rates) o DNA restriction o DNA sequence analysis
o Tissue sampling (DNA, total digestion & ligation o Gene & recombinant DNA
protein, zoox density) and snap o DNA preparation for cloning
freezing. sequencing o Chemistry Laboratory
o Mass spectrometer o DNA purification Skills including TLC,
(spectrophotometer) o cDNA synthesis NMR, extractions,
o Genomic DNA & RNA o qPCR & RT-qPCR distillation, etc.
isolation
Field Sampling
o Instrument handling (CTD, plankton net, o Water filtration (glass, polycarbonate &
sediment grab, & rosette). Steryvex).
o Recording of data accurately. o Sample collection & fixation (DIC, DNA,
o YSI measurements of Temperature, Salinity, DOC, nutrients, O2, proteins, RNA, SAGs &
Conductivity and pH. salinity).
o Coral and planulae collection.
Computer
o RStudio for statistics. o HOBOware.
o Image J for image analysis. o Microsoft Office: Word, Excel and
o Adobe Photoshop CC 2017. PowerPoint.
o Plot: RStudio Programming and Matlab.
Communication
o Bilingual – Fluent in English and Spanish. o Presentation of research methods, progress &
o Scientific writing of formal reports. results.
CERTIFICATIONS