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SUBMITTED BY:
Valdez, Loisroi R.
SUBMITTED TO:
Professor
December 2016
1
Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
Table of Contents
Chapter I- Introduction
I. Background of the Study 3
II. Statement of the Problem 5
III. Objectives 6
IV. Significance of the Studies 8
V. Scope and Limitations 9
Chapter II- Revie of Related Literature and Studies
I. Revie of Related Literature 10
II. Review of related Studies 20
Chapter III- Experimental Study
I. Procedure 22
II. Data Analysis
III. Summary of Parameters
Chapter IV- Conclusion and Recommendations
I. Conclusion
II. Recommendation
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
CHAPTER I
INTRODUCTION
Statistical data, the Philippines domestic and import usage of plastics has been
continuously increasing.
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
therefore comply with materials that are eco-efficient and sustainable. Among
the biopolymer matrices being utilized to produce bioplastics, starch is
considered the most widely used material.
In bioplastic production, 50% are starch based and the remaining are cellulose
and protein based. Starch based bioplastic can use corn kernels, sugar cane,
newspaper, plant scraps and banana peels as sources of starch.
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
The Philippines is the third largest exporter of bananas after Ecuador and
Columbia, with some 2.6 m tonnes exported in 2009. That year, the exports from
the Philippines (essentially Cavendish cultivars) made up 98% of the Asian
banana trade. Two thirds of the exported volumes were shipped to Japan, China
and South Korea.
In 2015, the country produced nearly 9.1m tonnes of bananas on 443,270 ha,
with Cavendish cultivars accounting for about 50% of national banana
production, Saba (29%) and Lakatan (11%). Latundan (a Silk cultivar) and other
cultivars accounted for about 11%. At the beginning of the century, as many as
90 cultivars were estimated to be grown for local consumption.
Among those, banana peels are waste and is the best option in choosing of raw
material. Also, banana production increased by 2.8% in 2014 to 8.88 million
metric tons (MT), per Bureau of Agricultural Statistics’ (BAS) which make it an
abundant source in the country. These are found to have minimum 15% starch
when immature and 30-40% when ripe.
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
During recent decades, there has been a continuous increase in the use of
plastics and it has become the major new material replacing some traditional
ones such as paper, steel and aluminum in many applications. The main
advantages of plastics are their low cost and lightweight. In addition, they are
easy to formulate and require low energy for their transportation and production.
The ever-growing production and use of plastics have led to a waste disposal
problem because, generally, they are inherently inert to the microorganisms or
the chemicals in an environment (Prinos, et al. 1998). Thus, they cannot degrade
when exposed to the environment. Conventional garbage disposal methods such
as incineration, landfill and recycling are not so attractive due to their respective
limitations. Incineration needs high temperatures of more than 800o C, which
makes it rarely used nowadays. Landfill has some problems of odor and the
scattering of lightweight waste materials by the wind. Recycling has not yet
gained widespread acceptance because of its difficulty in classifying and
separating the types of used plastics. For these reasons, there has been an
increased interest in the production and use of fully biodegradable polymers
replacing nonbiodegradable plastics
III. OBJECTIVES
GENERAL: The main objective of the experiment is to extract the starch from
the banana peels to produce a bioplastic sheet that conforms with the
standard properties.
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
SPECIFIC:
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
The study utilizes banana peels as its major raw material for the production of
bioplastic. This will provide the factory owners a potential market for bioplastic
rather than ending up the banana peelings as waste.
Both the materials and procedure of this study could be utilized and developed
by other institutions and could be a reason for a local production of bioplastic
from the raw material. Moreover, this study would help create jobs for local
citizens.
To the Students
The concepts used and the manufacturing process, as well as the literature
gathered in this experiment could be used as reference for further studies related
to the development of banana starch based bioplastic. Students could also use
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
the process described in the experiment to observe the conversion of banana peel
to a bioplastic in a simplified laboratory set-up.
This study may serve as an additional reference for related and similar studies
of fellow researchers. Chemical engineers could help in conducting further
research and study of the process involved in the production of bioplastic. With
the help of this field, scientific and economic condition in the country could be
improved.
Temperature
Time
Type of solvent
Ratio of a material to reagent
Concentration of reagents
Type of catalyst
Molar ratio
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
CHAPTER II
REVIEW OF RELATED LITERATURE & STUDIES
Banana production increased by 2.8% in 2014 to 8.88 million metric tons (MT),
according to the Bureau of Agricultural Statistics’ (BAS) Major Crops Bulletin,
but this year’s output could suffer a setback as the prevailing mild El Niño has
started to affect harvest.
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
The relationship between peel color and starch index, according to our chart,
shows a reasonable positive linear correlation
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
The relationship between pH changes and starch index is not linear, and best
fits an exponential curve (Fig. 4). During normal banana ripening, the starch-
iodine staining technique for assessing pulp ripe ness correlates well with color
and soluble solids. Use of the technique to evaluate pulp maturity should be of
value to both researchers and workers in the banana industry in evaluations
when internal ripeness is more important than appearance, when color is not a
usable index, or when temperature and humidity problems arise and external
and internal ripening are not well-correlated
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
boats. There are more than 300 differentypes of plasticizers of which about 50-
100 are in commercial use. The most commonly used are phthalates and
adipates.
Usually, the second major component of a starch based film is the plasticizer,
which is used to overcome film brittleness caused by high intermolecular forces.
Plasticizing agents commonly used for thermoplastic starch production include
water and glycerol (Alves et al., 2007, Famá et al., 2006, Famá et al., 2007,
Jangehud and Chinnan, 1999, Mali et al., 2006 and Parra et al., 2004),
polyethylene glycol (Parra et al., 2004) and other polyols, such as sorbitol,
mannitol and sugars (Kechichian et al., 2010, Talja et al., 2008 and Veiga-Santos
et al., 2008).
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
Starch consists of two different types of polymer chains, called amylose and
amylopectin, made up of adjoined glucose molecules. The hydrochloric acid is
used in the hydrolysis of amylopectin, which is needed in order to aid the process
of film formation due to the H-bonding amongst the chains of glucose in starch,
since amylopectin restricts the film formation. The sodium hydroxide used in
the experiment is simply used in order to neutralize the pH of the medium.
The 9th and 10th pilot experiment conducted had been successful in producing
plastic, but had started to decay after only 3 days. As a result of the research
done to address this issue, I found out that in order to improve shelf life of post-
harvest wild mango fruits, sodium metabisulphite can be used (Ibadan, 1991).
This is why the sodium metabisulphite solution was used in this experiment.
Starch from cereals (wheat, maize and finger millet) pulses (chick pea and green
gram), tuber (potato) and root (tapioca) was modified with different acids (0.5 N,
1.5 h, 50°C). Molecular weight (number average, Full-size image (<1 K)) of these
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
starches decreased after modification, H3PO4 causing the least and HCl and
HNO3 the highest reduction. Gel permeation chromatography of native
starches using Sepharose CL 4B gave mainly two fractions. Fraction I (Fr. I), a
higher molecular weight component eluting in the void volume and Fraction II
(Fr. II), a lower molecular weight component that entered the gel and eluted at
higher elution volumes. After acid modification, the carbohydrate content of Fr.
II increased while that of Fr. I decreased. The magnitude of the effect for different
acids followed the same pattern as was the case for molecular weight. Very high
increase in the total carbohydrate content in Fr. II was seen in cereal starches
followed by pulses, root and least by tuber. The λmax values of the peak of Fr. I
increased in cereal and millet starches after modification by 9 to 14 nm, but
either remained the same or decreased to some extent in other starches. The
peak of Fr. II of modified starches had similar Kav to that of the respective native
starch suggesting that the degraded portion had a molecular size similar to that
of Fr. II of the native starch. However, the λmax of the peak of Fr. II decreased
after modification indicating that degraded portion of Fr. I which entered the gel
and eluted with the peak of Fr. II was branched.
II. Process
Acid hydrolysis is an important chemical modification that can significantly
change the structural and functional properties of starch without disrupting its
granular morphology. During acid hydrolysis, amorphous regions are hydrolyzed
preferentially, which enhances the crystallinity and double helical content of acid
hydrolyzed starch. The effects of acid hydrolysis on amylose content, chain
length distribution of amylopectin molecules, molecular and crystalline
organization (including lamellar structure) and granular morphology are
considered. Functional properties discussed include swelling power,
gelatinization, retrogradation, pasting, gel texture, and in vitro enzyme
digestibility.
III. Product
Starch Based Plastic
Starch is considered to be a biodegradable polymer and can be used for the
production of starch-based resin (Takagi, Ichihara, 2004) bioplastics. Starch
when harvested is turned into a white, granular product. According to the
Australian Academy of Science, “starch can be processed directly into a
bioplastic, but because it is soluble in water, articles made from starch will swell
and deform when exposed to moisture, limiting its use” (Packaging Greener,
2004). The starch must be transformed into an altered polymer in order to solve
the issue of starch deformation. Biodegradable starches can be processed “using
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
Biodegradability of Bioplastic
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
V.
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
length which is caused by the pulling. Figure 1 below shows a typical curve that
results from a tensile test.
This investigation will use tensile and elongation testing to differentiate between
various polymers including plastics and elastomers.
1. Using a permanent marker, mark the samples 5 cm from one end across the
width of the sample. From this marking, measure 10.0 cm and mark the sample
across the width of the sample. The area between the marks is the elongation
test area. Mark all test samples.
2. Tape a meter stick or a metric tape measure to the lab table top. Place the
first test sample so that one of the marked lines is lined up with the zero point
of the measuring device. Use the heel of your hand to hold it in place such that
the heel of your hand and the marking are in line with the zero point.
3. Measure the distance to the second line to 0.1 cm and record it as the initial
length.
4. Holding the sample just beyond the second line, slowly stretch the as far as
possible without it breaking. Record the final length between the marked lines.
Allow the sample to relax slowly without completely releasing it. Be careful not
to release the stretched elastomer, snapping your hand or another student.
7. Repeat for a total of 3 of the same samples and calculate the average percent
elongation
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
Biodegradability Test
When testing the degradation phenomena of plastics in the environment, there
is a general problem concerning the type of tests to be applied, and the
conclusions which can be drawn. In principle, tests can be subdivided into three
categories: field tests; simulation tests; and laboratory tests (Figure 2). Although
field tests, such as burying plastics samples in soil, placing it in a lake or river,
or performing a full-scale composting process with the biodegradable plastic,
represent the ideal practical environmental conditions, there are several serious
disadvantages associated with these types of test. One problem is that
environmental conditions such as temperature, pH, or humidity cannot be well
controlled; secondly, the analytical opportunities to monitor the degradation
process are limited. In most cases it is only possible to evaluate visible changes
on the polymer specimen, or perhaps to determine disintegration by measuring
weight loss. The latter approach is problematic however if the material breaks
into small fragments that must be quantitatively recovered from the soil,
compost or water. The analysis of residues and intermediates is complicated by
the complex and undefined environment. Since the pure physical disintegration
of a plastic is not regarded as biodegradation in the sense of most definitions
(see above), these tests alone can never prove whether a material is
biodegradable, or not. As an alternative to field tests, various simulation tests in
the laboratory have been used to measure the biodegradation of plastics. Here,
the degradation might take place in compost, soil or sea-water placed in a
controlled reactor in a laboratory. Although the environment is still very close to
the fieldtest situation, the external parameters (temperature, pH, humidity, etc.)
can be controlled and adjusted, and the analytical tools available are better than
would be used for field tests (e.g., for analysis of residues and intermediates,
determination of CO2 evolution or O2 consumption). Examples of such tests
include the soil burial test (Pantke and Seal, 1990), the so-called ™controlled
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
composting test∫ (Pagga et al., 1995; Tosin et al., 1996; Degli-Innocenti et al.,
1998; Ohtaki et al., 1998; Tuominen et al., 2002), test simulating landfills
(McCartin et al., 1990; Smith et al., 1990; McCarthy et al., 1992) or aqueous
aquarium tests (P¸chner et al., 1995). On occasion, in order to reduce the time
taken to conduct the tests, nutrients are added to increase the microbial activity
and accelerate degradation. The most reproducible biodegradation tests are the
laboratory tests, where defined media are used (in most cases synthetic media)
and inoculated with either a mixed microbial population (e.g., from waste water)
or individual microbial strains which may have been especially screened for a
particular polymer. In such tests, which may be optimized for the activity of the
particular microorganisms used, polymers often exhibit a much higher
degradation rate than would be observed under natural conditions
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
Production” by Dr. Been Huang Chiang et al., low temperature milling decreases
the viscosity of the resulting starch adhesive, also by addition of 0.05M NaOH
increases the efficiency of starch extraction. This extraction yields 70% starch
and of approximately 94% purity.
The process can also be applied in obtaining starch from banana peels alone.
Some of the parameters used and the principles can be used; such were the
concentration of alkali used, the type of alkali, the preliminary treatment to the
banana peels, and the succeeding procedures including the equipment used.
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
CHAPTER III
EXPERIMENTAL STUDY
A. WASHING
Washing is done to remove the dirt, and other impurities that adhere to the tuber
during and after peeling. The banana peel is washed with water
c. Objective
d. Hypothesis
e. Procedure
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
1.Place 250 grams peeled banana peel in the basin and spray
for 50 mL of water in 5 minutes
2.Weigh the washed tuber.
3.Repeat the procedure four times.
4.Record the appearance of wash water per trial
The water is
lighter compared
to the first one.
There are still peel
particles
suspended on it.
g. Analysis
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
h. Conclusion
B. CUTTING
Banana Peel
Water
Knife
Blender
Analytical balance
Stopwatch
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
c. Objective
d. Hypothesis
The smaller the size of banana peel, the better the
homogeneity of the mixture produced.
e. Procedure
1. Weigh five samples of 250 g washed banana peel.
2. Cut one of the tubers into cubes with a size of 0.5 inch. Cut the
remaining four tubers into sizes of 0.5, 1 and 1.5 cm thick.
3. Add water to the banana cubes using 1:1 mass ratio.
4. Blend for 5 minutes using a blender.
5. Observe the homogeneity of the mixture.
f. Analysis
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
g. Conclusion
Thickness of 0.5 cm of peeled banana to is sufficient enough
to produce the desired smoothness and homogeneity of the
banana slurry.
Banana Peel
Water
Knife
Blender
Analytical balance
Stopwatch
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Pamantasan ng Lungsod ng Maynila
College of Engineering and Technology
Department of Chemical Engineering
b. Objective
c. Hypothesis
d. Procedure
Amount Wt. of
of Banana
Trial Wt. of Cut
Banana Water Slurry
Observation
Added
Tubes (g) Produced
(mL) (g)
f. Analysis
g. Conclusion
Objective
To prolong the shelf life of the organic material
Procedure
1. Prepare 0.5M solution of Sodium metabisulfite by mixing 95.05g of sodium
metabisulfite in a liter of distilled water
2. Mix the solution throughly
3. Dip the banana peels in the solution for 30 minutes
4. Compare the dipped banana peel in a banana peel that is not soaked in
Sodium metabisulfite
II. Extraction
Boiling
The banana peels that were soaked in the sodium metabisulfite solution will be
boiled to extract the starch from the organic material. Residue and weight of
water will be recorded in this process.
Objective
Procedure
4. The solution will be heated to 80C for 30 minutes for the extraction of starch
Mashing
The dried banana peels will be mashed to create a puree solution for an easier
formation of the bioplastic in the petri dish
Objective
Procedure
3. If the banana peels are not crushed enough, a blender or a food processor is
recommended for further crushing
Procedure
1. The puree will be placed in a 50mL beaker
2. 0.5M of HCl will be added in the solution
3. After adding, the solution will be mixed by using a stirring rod.
3. Store the mixture for 1 hour before filtering the starch solution from
the mixture.
4. Wash the residue with water in the ratio of 1:1.5. Observe the total
time of filtration.
5. Collect the filtrate and centrifuge it in 1 minute interval, measuring
the height of precipitate formed in between until it come into a constant
height of precipitate.
6. Filter the starch precipitated and dry it. In between 10 minute
interval of drying, weigh the precipitate until it will come into a constant
mass.
7. Tabulate the results of centrifuge and drying.
Parameters
Weight of
banana 1000
peelings
Weight of
Weight of Time of Time of
Washing of filtrate Residue
wash washing, filtration,
Slurry collected, collected
water, g min min
g
Washing 1500 7 15 1667.4 832.6
height of solid
Time, min % Solid Content=( x 100)
height of total content
1 7.69
2 18.46
3 32.31
4 49.23
5 66.15
6 66.15
70
60
50
% Solid Content
40
30 Precipitate
Formed
20
10
0
1 2 3 4 5 6
Time
160
155
Weight of Dried Starch
150
Weight
145
of
Dried
140 Starch
135
130
0 10 20 30 40 50
Time
Addition of base
Since the mixture is acidic because of the acid, a base will be able to
neutralize the solution
Objective
To neutralize the solution and to prevent the solution from being acidic
Procedure
1. Check the pH of the solution by using a litmus paper
2. Once the litmus paper indicated an acid solution, a NaOH solution is
needed to be added
3. Add 3mL of 0.5M NaOH solution
4. After addition, check the acidity again by using a litmus paper
Blending/Crushing
Addition of NaOH
Parameters
Washed banana
200 200 200
peelings, g
Weight of base, g 600 600 600
Weight of wash
300 300 300
water, g
Weight of residue, g 165.2 170.6 173.2
Weight of filtrate, g 934.8 929.4 926.8
1200
800
600
934.8 929.4 926.8
400
200
0
NaOH KOH Ba(OH)2
Filtrate Residue
Analysis: Among 3 bases, NaOH, KOH, and Ba(OH)2, NaOH yielded the
most amount of starch solution.
180 178
175
170 168.6
165.2
165
160
155
0.01M 0.05M 0.10M
Weight of Residue
CHAPTER IV
CONCLUSION & RECOMMENDATION
A. Conclusion
B. Recommendation
REFERENCES
A Pilot Scale Study for Banana Starch Production. (n.d.). Retrieved February 02,
2017, from
http://onlinelibrary.wiley.com/doi/10.1002/star.19870390103/abstract
Effect of processing on the viscoelastic, tensile and ... (n.d.). Retrieved February
2, 2017, from https://www.scipers.com/cPaper-View-Effect-of-processing-on-
the-viscoelastic-tensile-and-optical-properties-of-albumenstarch-based-
bioplastics.html