Cells - RioSalado - AZ Question Answer All living cells - what 2 catagories? Prokaryotes & Eukaryotes DNA in single, circular arranged chromosome Structural characteristics of w/o membrane - lack membrane - enclosed prokaryotes. organelles Structural characteristics of Multiple chromosomes in membrane-enclosed eukaryotes. nucleus. Bacteria & Archaea are? Prokaryotes Protozoa & fungi are? Eukaryotes What main difference is there Structure of cell walls & membranes, & absence between prokaryote & eukaryote? of organelles. (1) No DNA membrane, (2) DNA w/no 5 distinguishing characteristics of histones, (3) lack membrane-enclosed prokaryotes. organelles, (4) cell wall w/polysaccharide peptidoglycan, & (5) binary fission. DNA copied & cell splits into 2 - prokaryotes - Binary fission fewer processes than eukaryote cell division. (1) DNA in nucleus w/membrane & multiple 5 distinguishing characteristics of chromosomes, (2) DNA associated w/histone, eukaryotes. (3) membrane-enclosed organelles, (4) cell walls chemically simple, (5) mitosis. 3 basic shapes of bacteria Coccus (berries), bacillus (rod-shaped), & spiral Diplococci Cocci that remain in pairs after dividing. Cocci that divide & are attached in chainlike Streptococci pattern. Cocci that divide in 2 planes & remain in groups Tetrads of 4. Cocci in 3 planes & remain in cubelike groups Sarcinae of 8. Cocci in multiple plains & form grapelike Staphylococci clusters. Why are there fewer groupings of Because bacilli only divide across short axis bacilli than cocci? Diplobacilli Appear in pairs after division Streptobacilli Appear in chains after division Coccobacilli Oval bacilli that look like cocci. Bacillus cells often form __. long, twisted chains of cells. What is the difference between One is bacterial shape & one is genus. bacillus & Bacillus? Vibrios Spiral bacteria that look like curved rod. Spirilla Spiral bacteria that have helical shape Spiral bacteria that have helical shape & are Spirochetes flexible - move by axial filaments. Monomorphic Maintain a single shape What prokaryotes are Bacteria monomorphic? Structures external in prokayotic Glycocalyx, flagella, axial filaments, finbriae & cell wall. pili. "Sugar coat" - secreted on surface of Glycocalyx prokaryotes - viscous - if firm, then called capsule. Firmly attached sugar coat around prokaryote Capsule that can be determined by using negative staining. Loosely attached glycocalyx (sugar coat) around Slime layer prokaryote. Capsules are important in virulence - degree of disease causing ability. contributing to bacterial __. Capsules often protect pathogenic From phagocytosis by cells of host. bacteria __. Glycocalyx made of sugars - enables bacteria to Extracellular polysaccharide (EPS) survive by attaching to various surfaces. Atrichous Bacteria that lack flagella Monotrichous Single polar flagellum 3 basic parts of flagellum Filament, hook, basal body Protein that makes up flagellum flagellin filament. Difference between prokaryote & Most bacteria lack membrane/sheath covering it. eukaryote flagella. What anchors flagellum to cell wall Movement away from/toward a particular & plasma membrane? stimulus. How do spirochetes & spirilla Spirochetes move using axial differ? filaments/endoflagella. Axial filaments - bundles of fibrils that arise at Endoflagella ends of cell beneath outer sheath & spiral around cell. Axial filaments propel spirochetes in a spiral motion like a cork screw. __. Appendage on bacterial cell used to adhere to fimbriae surfaces - helps colonization. Longer than fimbrae & join bacterial cells for pili transfer of DNA. Process by which bacteria use pili to transfer conjugation DNA from one cell to another. What is the function of fimbriae? to adhere to surfaces Almost all prokaryotes have __. cell walls Prevent rupture when water pressure in cell is Major function of cell wall. greater than outside. What is used to differentiate major The chemical composition of the cell wall. types of bacteria? Gram-positive cell walls contain? Many layers of peptidoglycan. Gram-negative cell walls do not teichoic acids contain __. Which is more susceptible to Gram negative because they only contain a mechanical breakage? Gram - or +? small amount of peptidoglycan. The outer membrane of gram- Certain antibiotics & digestive enzymes. negative provides barrier against? Smallest known bacteria outside Mycoplasmas - pass through most filters. living host. Archaea may lack cell walls but Peptidoglycan - have pseudomurein instead. definately lack __. Acid-fast bacteria cell walls contain Mycolic acid - resists dyes because it is waxy. what lipid? Why are prokaryote plasma membranes less rigid than Because they lack sterols. eukaryotes? Infolding of plasma membrane where pigment Chromatophores located in photoautotrophic bacteria. How does simple diffusion differ Both don't need ATP, but facilitated uses from facilitated? transporter. Net movement of solvent molecules across What is osmosis? selectively permeable membrane from high to low concentration of solvents. In isotonic solution, movement is? No net movement of water In hypotonic solution, movment is? Water moves into cell & may cause cell to burst. In hypertonic solution, movment is? Water moves out of cell, causing shrinkage. Osmotic lysis Water moving into a cell & it bursts. Plasmolysis Water moving out of cell & it shrinks. Pressure required to prevent movement of pure Osmotic pressure water into solution containing some solvents. Most bacteria live in __ solutions. hypotonic Only in prokaryotes - substance is chemically Group translocation altered as it passes through plasma membrane & can no longer leave - high energy PEP. Difference between prokaryote & The differences allow the cell to be killed by the eukaryote ribosomes regarding antibiotic, yet leave eukaryote host unaffected. antibotic therapy. Reserve deposits in prokaryote cytoplasm of Inclusions nutrients - good ID tag. Volutin Reserve of inorganic phosphate for ATP. Nuclear area of bacterial cell containing Nucleoid bacterial chromosome. Circular DNA molecules that replicate independent of chromosomal DNA - genetically Plasmids not crucial to bacterial survival except during adverse conditions. Highly durable dehydrated cells - almost seed- Endospores like Under what conditions are When essential nutrients are depletes in certain endospores formed by bacteria? gram-positive bacteria. Sporogenesis/sporulation Process of endospore formation When endospore returns to health later - water Germination enters. What kingdoms contain eukaryote Alge, protozoa, fungi, plants, & animals. organisms. What is antibiotic significance of Penicillins & cephalosporins act against eukaryote cells not containing peptidoglycan & therefore don't affect eukaryote peptidoglycan? cells. How do plasma membranes differ Prokaryote lack sterols and carbs. between eukaryote and prokaryote. cytosol refers to fluid portion of cytoplasm Movement of cytoplasm in cell that helps cytoplasmic streaming distribute nutrients & move a cell over a surface. Many enzymes found in cytoplasmic fluid of prokaryote are Organelles sequestered in __ of eukaryote. Movement of substances between nucleus & Nuclear pores control __. cytoplasm. Condensed regions of chromosomes where Nucleoli/nucleolus rRNA is being synthesized. Cisterns Flattened membrane sacs of ER. ER contains __ & synthesizes __. unique enzymes - phospholipids, fats & sterols. What are the functions of golgi Package substances in vesicles for transport. complex? Cristae Outer mitochondrial membrane __ are organelles that can reproduce mitochondria more or less on their own. Many of the metabolic steps involved in cellular respiration are matrix of mitochondria concentrated in __. Organelle in algae & green plants that contain Chloroplast chlorophyll & enzymes Chlorophyll is contained in what thylakoids sacs? __ in plants can reproduce on own chloroplasts like mitochondria. How are mitochondria similar to How they reproduce - increase in size - then prokaryote cells? divide in two. Contain enzymes that can oxidize various peroxisomes organic substances - aminoacids & fatty acids. Peroxisomes protect other cell parts H2O2 - hydrogen peroxide from __. Centrioles & pericentricular material - miotic Centrosome spindle organizing center. Larger bacterial cell lost their cell walls & Endosymbiotic theory engulfed smaller bacterial cells & so eukaryotes formed. Similar to bacterial cells, both mitochondria & chloroplasts contain circular DNA & can reproduce on own. __.