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Purpose of the Course
This course aims to provide a detailed introduction to molecular biology, including proteins,
carbohydrates, lipids, and nucleic acids; water; and other compounds that make up the cell. Particular
emphasis is on the cell membrane, mitosis and meiosis, genetics and gene expression, transcription
and translation, and cellular metabolism.
Course Objectives
The objectives of this course are to:
1. Explain basic molecular biology concepts, including its origins
2. Examine how cells break down and build biomolecules
3. Describe cellular process responsible for DNA replication
4. Discuss evolution at the level of the gene and genome
Course Content
This course is an introduction to basic molecular biology, including its origins. Introduces the molecules and
molecular processes underlying life. Overview of structures and chemical properties of biological
macromolecules. The chemical basis of life, metabolism, DNA replication and transcription, protein synthesis,
and mechanisms of gene regulation.
II. Methods
Isolation and Purification of Proteins
Isolation of DNA and RNA
Chromatography and Electrophoresis of Nucleic Acids
Hybridization of Nucleic Acids
Use of Enzymes in the Modification of Nucleic Acids
Polymerase Chain Reaction
DNA Sequencing
Cloning Procedures
Expression of Recombinant Proteins
END