e154 Abstracts
POSTER SESSION
S
U
N
D 3921.4 ± 538.7 mmol/l, p < 0.05). On the other hand, markers of nitrative stress
POSTERS’ SESSION PS13:
A AGEING, CHILDREN AND
Y p < 0.05; leukocyte NT: H: 90 [86; 96] vs. L: 77 [55; 92]%; p < 0.05).
ADOLESCENTS, WOMEN
P Conclusions: Based on our data, coincidence can be assumed for lower plasma
O total peroxide levels, higher tyrosine nitration and elevated artery uterine resis-
S bioavailability in the high resistance group. The lower total plasma peroxide level
T NITRIC OXIDE, SERUM HOMOCYSTEINE AND INTIMA-MEDIA
E THICKNESS IN ADOLESCENT HYPERTENSION
R Support: OTKA_PD 113022
S. Lengyel, E. Katona, C. Molnar, G. Paragh, B. Fulesdi, D. Pall. University of
S Debrecen, Debrecen, HUNGARY
Objective: It is well known, that adult hypertensives have lower nitric oxide, high-
er serum homocysteine levels, and increased intima-media thickness. We investi-
gated whether these abnormalities are also detectable in adolescent hypertension.
Design and method: 59 normotensive, 47 white coat hypertensive and 73 sus-
tained hypertensive adolescents were included in our study. Plasma NOx as well
as homocysteine levels were measured with validated and standardized labora-
tory methods. The intima-media thickness (IMT) of the common carotid artery
was defined by measuring the distance between the media-adventitia layer and the
lumen-intima interface. Spearman correlation was applied for the assessment of
the relationship between homocysteine and IMT.
Results: The three groups had similar mean age (15.8 ± 0.6 years; 16.3 ± 1.1 years;
16.5 ± 1.0 years, p = NS). Compared to normotensives (38.8 ± 7.6 umol/l), both
white-coat hypertensive (30.6 ± 11.0 umol/l) and sustained (28.7 ± 22. 4 umol/l)
hypertensive adolescents had significantly lower nitric oxide levels (p < 0.001).
We observed significantly (p < 0.05) higher homocysteine levels in adolescent
white-coat hypertension (11.6 ± 6.8 umol/l) and adolescent sustained hypertension
(12.1 ± 7.0 umol/l) compared to normotension (9.8 ± 3.1 umol/l). IMT was higher
in both white-coat (0.056 ± 0.001 cm) and sustained hypertensive adolescents
(means 0.054 ± 0.001 cm) compared with controls (0.048 ± 0.001 cm). Not signif-
icant difference was observed in the two hypertensive group. In the two hyperten-
sive groups between serum homocysteine levels and carotid artery intima-media
thickness a significant positive correlation was observed (r = 0.43; p < 0.01).
Conclusions: We were able to confirm that not only the sustained, but also the
white coat hypertensives have endothelial dysfunction and subclinical target organ
damage.
INVESTIGATING OXIDATIVE-NITRATIVE STRESS TOGETHER WITH
ARTERIAL UTERINE RESISTANCE MEASUREMENT IN HEALTHY
PREGNANT WOMEN
E.M. Horvath1, R. Benko1, Z. Mezei1, A. Penyige1, D. Gerszi1, B. Sarai-Szabo1,
C. Demendi2, S. Varbiro2. 1Semmelweis University, Department of Physiology,
Budapest, HUNGARY, 2Semmelweis University, 2nd Department of Obstetrics
and Gynecology, Budapest, HUNGARY
Objective: Alterations in placental circulation during pregnancy that may lead to
higher circulatory resistance can be predictive for preeclampsia. Measuring the
resistance of the uterine artery at the end of the first trimester may contribute to
the identification of higher risk moms. As increased oxidative-nitrative stress may
also play role in the development of pregnancy-related hypertension, our study
aimed to interconnect these two processes (changes in the resistance of uterine
artery and oxidative-nitrative stress).
Design and method: Healthy pregnant women were recruited at the 2nd Depart-
ment of Obstetrics and Gynecology during the 10–13th week ultrasound examination.
Based on the uterine artery pulsatile index, participants were divided into two groups:
high resistance (H, n = 30) and low resistance (L, n = 31) with the cutoff point of 2.4.
Besides clinical laboratory parameters signs of oxidative-nitrative stress were investi-
gated in blood samples; plasma total peroxide, nitrotyrosine (NT), and isolated mono-
nuclear leucocyte tyrosine nitration were determined. Pregnancies were followed; pos-
sible adverse events and anthropometric data of the newborns were registered.
Results: Age and BMI was similar in the two groups, however parity was sig-
nificantly lower in the high resistance group (H: 0.40 ± 0.81 vs. L: 1.03 ± 1.08,
p < 0.05). Plasma total peroxide level reflecting systemic oxidative stress
was significantly lower in the high resistant group (H: 3435.6 ± 809.3 vs. L:
Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved
Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.
like plasma and leukocyte protein tyrosin nitration was significantly higher in the
high resistance group (plasma NT: H: 1751.7 ± 2007.9 vs. L: 710.2 ± 436.6 nmol/l,
tance. These results may suggest the alteration of nitric oxide metabolism and
of these patients however may reflect a delay in the first increase in oxygen tension
of the placenta during its development that may also effect trophoblast invasion.
MESENCHYMAL STEM CELLS INFLUENCE TROPHOBLAST
AND ENDOTHELIAL CELL FUNCTIONALITY IMPORTANT
FOR PREVENTION OF PRE-ECLAMPSIA VIA A NOVEL ANTI-
ANGIOGENIC PROTEIN, FKBPL
N. Todd, R. Mcnally, A. Alqudah, A. Krasnodembskaya, L. Mcclements. Centre of
Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences,
Queens University Belfast, Belfast, UNITED KINGDOM
Objective: Pre-eclampsia is a disorder affecting 5–6% of all pregnancies globally.
It is characterised by the new onset of hypertension and proteinuria post 20 weeks
gestation. Pre-eclampsia is a leading cause of morbidity and mortality in both mothers
and children. Although the pathogenesis of pre-eclampsia is poorly understood, aber-
rant angiogenesis and inadequate trophoblast cell function have both been implicated.
Mesenchymal Stem Cell (MSC)-based therapies have shown benefits in animal mod-
els of pre-eclampsia however, the underlying mechanisms are not well understood.
FKBPL is a novel anti-angiogenic protein which has a critical role in developmental,
physiological and pathological angiogenesis. In this study, our objective was to evalu-
ate the effects of MSC–conditioned medium (MSC-CM) on migration and differentia-
tion of trophoblast and endothelial cell lines under both normoxic and hypoxic condi-
tions. The role of FKBPL signalling in these processes was also investigated.
Design and method: Human trophoblast (BeWo and Jar) and endothelial cells
(HUVEC) were incubated in the presence of human bone marrow- derived MSC-
CM, normal or serum free medium under normoxia (21% oxygen) or hypoxia (1%
oxygen). The confluent cell monolayer was wounded and the percentage of wound
closure assessed at 24 h. HUVEC were stained with Calcein prior to 6-hour incuba-
tion in the presence of MSC-CM, normal or serum free medium under normoxia or
hypoxia. Tubule formation was assessed using Image J. FKBPL protein expression
was evaluated using western blot analysis where cells were lysed in RIPA buffer
before being subjected to western blotting and probed for FKBPL and GAPDH.
Results: MSC-CM promoted cell migration significantly in all three cell lines
under both normoxia and hypoxia compared to normal medium (n = 6; p < 0.001).
MSC-CM also significantly increased the formation of HUVEC tubule networks
under both normoxia and hypoxia compared to normal medium. Furthermore,
concomitant reduction in FKBPL protein expression was observed, demonstrating
potential FKBPL’s involvement in MSC-driven effects on trophoblast and endo-
thelial cell functionality.
Conclusions: Our findings suggest that MSCs could be explored as potential
preventative therapy for pre-eclampsia by ameliorating trophoblast and endothe-
lial cell functionality, and that the mechanism is likely to involve a novel anti-
angiogenic protein, FKBPL.
VASCULAR AGEING AND PRECLINICAL TARGET ORGAN DAMAGE
IN COMMUNITY-DWELLING ELDERLY: THE NORTHERN SHANGHAI
STUDY
Y. Zhang1, H. Ji1, J. Xiong1, S. Yu1, C. Chi1, T. Jiadela1, J. Li2, J. Blacher3, Y. Xu1.
1Department of Cardiology, Shanghai Tenth Peoples Hospital, Tongji University
School of Medicine, 301 Yanchang Road, Sha, Shanghai, CHINA, 2Institute of
Clinical Epidemiology and Evidence-Based Medicine, Tongji University, Shang-
hai, 200092, China, Shanghai, CHINA, 3Paris Descartes University; AP-HP,
Paris, FRANCE
Objective: Vascular aging represents a mediating step between risk factors and
cardiovascular (CV) events. And preclinical target organ damage (TOD) inte-
grates the cumulative effects of CV risk factors with aging which can be detected