UNIVERSIDAD TÉCNICA DEL NORTE

Facultad de Ingeniería en Ciencias Agropecuarias y Ambientales

INGENIERÍA AGROINDUSTRIAL

BIOTECNOLOGÍA

Nombre: Silvia Tuquerrez

Fecha: 25 de julio de 2018

EJERCICIO DE FILTRACION

A uspension of Bacillus subtilis cells is filtered under constant pressure for recovery of
protease. A pilot-scale filter is used to measure the filtration properties. The filter area is
0.25 m2, the pressure drop is 360 mmHg, and the filtrate viscosity is 4.0 cP. The cell
suspension deposits 22 g of cake per litre of filtrate. The following data are measured.

Time (min) 2 3 6 10 15 20
Filtrate volume (L) 9,8 12,1 18,0 23,8 29,9 37,5

DATOS

𝐴 = 0.25 𝑚2

101325𝑃𝑎
∆p = 360 𝑚𝑚𝐻𝑔 → ∆𝑝 = 360 𝑚𝑚𝐻𝑔 × = 47996,05263𝑃𝑎
760 𝑚𝑚𝐻𝑔

𝑃𝑎
𝜇𝑓 = 4𝑐𝑃 → 𝜇𝑓 = 4𝑐𝑃 × = 0.004𝑃𝑎. 𝑠
1000𝑐𝑃

𝑔 𝑔 𝑘𝑔 1000𝐿 𝑘𝑔
𝑐 = 22 →= 𝜇𝑓 = 22 × × = 22
𝐿 𝐿 1000𝑔 𝑚3 𝑚3
 (a) Determine the specific cake resistance and filter medium resistance. Para
determinar la resistencia específica de la torta (α ) y la resistencia del medio
filtrante (𝑅𝑚 ) se usa los datos obtenidos experimentalmente, y mediante un gráfico
se obtiene 𝐾1 𝑦 𝐾2 .

Time Filtrate volume t/vf

(min) (L) (min/L)
2 9,8 0,20
3 12,1 0,25
6 18,0 0,33
10 23,8 0,42
15 29,9 0,50
20 37,5 0,53

Gráfico t/Vf - Vf
0,60

0,50

0,40

0,30

0,20

0,10
0,0 5,0 10,0 15,0 20,0 25,0 30,0 35,0 40,0

Vf (L)

𝑚𝑖𝑛 𝑚𝑖𝑛
𝐾1 = 0.0124 𝐿2
𝐾2 = 0.01027 𝐿2
 𝑎𝑝𝑎𝑟𝑡𝑖𝑟 𝑑𝑒 𝑒𝑠𝑡𝑜𝑠 𝑣𝑎𝑙𝑜𝑟𝑒𝑠 𝑜𝑏𝑡𝑒𝑛𝑒𝑚𝑜𝑠 𝛼 𝑦 𝑅𝑚

𝜇𝑓 𝛼𝑐 2𝐴2 ∆𝑝𝐾1
𝐾1 = →𝛼=
2𝐴2 ∆𝑝 𝜇𝑓 𝑐

𝑚𝑖𝑛 𝑠 (1000)2 𝐿2
2(0.25𝑚2) (47996,0526𝑃𝑎) (0.0124 ) (60 )
𝐿2 𝑚𝑖𝑛 (𝑚3 )2
α=
𝑘𝑔
(0.004 𝑃𝑎. 𝑠) (22 3 )
𝑚

𝒎
= 𝟓. 𝟏 × 𝟏𝟎𝟏𝟎
𝒌𝒈

𝜇𝑓 𝑅𝑚 𝐴∆𝑝𝐾2
𝐾1 = → 𝑅𝑚 =
𝐴∆𝑝 𝜇𝑓

𝑚𝑖𝑛 𝑠 1000 𝐿2
0.25𝑚2 (47996,0526𝑃𝑎) (0.0124 ) (60 )
𝑅𝑚 = 𝐿2 𝑚𝑖𝑛 𝑚3
(0.004 𝑃𝑎. 𝑠)
10 1
= 1.85 × 10
𝑚

Based on the data obtained in the pilot – scale study, what size filter is required to
process 4000 L of cell suspension in 30 min at a pressure drop of 360 mmHg?

𝑡 𝜇𝑓 𝛼𝑐 𝜇𝑓𝑅𝑚
= 𝑉𝑓 +
𝑉𝑓 2𝐴2 ∆𝑝 𝐴∆𝑝

𝑡 𝜇𝑓𝑅𝑚 𝜇𝑓 𝛼𝑐
𝐴2 − 𝐴− 𝑉𝑓 = 0
𝑉𝑓 ∆𝑝 2∆𝑝

𝜇𝑓𝑅𝑚 𝑉𝑓 𝜇𝑓 𝛼𝑐
𝐴 − 2
𝐴− 𝑉𝑓 2 = 0
𝑡∆𝑝 2∆𝑝
 1 𝑚3
𝜇𝑓𝑅𝑚 𝑉𝑓 (0,004𝑃𝑎) (1,85 × 10 10
) (4000𝐿)
𝑚 1000𝐿
= = 𝟑, 𝟒𝟑𝒎𝟐
𝑡∆𝑝 𝑠
30 𝑚𝑖𝑛 (60 ) ( 47996,0526𝑃𝑎)
𝑚𝑖𝑛
𝑚 𝑘𝑔
𝜇𝑓𝑅𝑚 𝑉𝑓 (0,004𝑃𝑎) (5,1 × 1010 ) 22 𝑚3 ( 𝑚3 )
2
𝑘𝑔 2
= (400𝐿) = 𝟒𝟏𝟓, 𝟔 𝒎𝟒
𝑡∆𝑝 𝑠 2
(1000 )𝐿 2
2(30 min) (60 ) ( 47996,0526𝑃𝑎)
𝑚𝑖𝑛

𝐴2 − 3,4𝑚2 𝐴 − 415,6𝑚4 = 0

−𝑏 ± √𝑏 2 − 4𝑎𝑐
𝐴=
2𝑎

−(−0,34𝑚2 ) ± √(−0,34𝑚2 )2 − 4(−415,6 𝑚4 )

A=
2

3,4𝑚2 ± 40,9𝑚2
A=
2
3,4𝑚2 + 40,9𝑚2
A=
2
A = 22,15 𝑚2