International Journal of Trend in Scientific
Research and Development (IJTSRD)
International Open Access Journal
ISSN No: 2456 - 6470 | www.ijtsrd.com | Volume - 2 | Issue – 2
Biosynthesis and Characterization ooff Zinc Oxide
Nanoparticles u
using
sing Onion Bulb Extract
N. Tensingh Baliah
Post Graduate and Research Department
of Botany, Ayya Nadar Janaki Ammal
College, Sivakasi, Tamil Nadu, India
ABSTRACT
The wide application of nanoparticles stimulates the
need for synthesizing them but, the conventional
methods are usually hazardous and energy
consuming. This leads to focus on “green synthesis”
of nanoparticles which seems to be easy efficient and
ecofriendly approach.
oach. In this study, the plant mediated
synthesis of zinc oxide nanoparticles (ZnO NPs) was
carried out using bulb extract of Allium cepa as a
reducing agent. The optimized nano zinc thus
obtained was quantified and characterized using UV UV-
Visible spectroscopy, Fourier Transform Infrared
Spectroscopy (FT-IR), X-Ray Ray diffraction, Scanning
Electron Microscope (SEM), EDAX and Zeta
potential analyses.. Further, the synthesized ZnO NPs
were tested for antimicrobial activity.
Keywords: Nanoparticles, zinc oxide, biosynthesis,
Allium cepa
I. INTRODUCTION
Nanotechnology is a emerging field with its
application in science and technology for the purpose
of manufacturing new materials at nano scale level
(Albrecht et al.,., 2006). Recent advance in the field of
nanotechnology,
ogy, particularly the ability to prepare
highly ordered nanoparticles
particles of any size and shape
(Sangeetha et al.,., 2012). Nanotechnology is a
multidisciplinary scientific field undergoing explosive
development. The nanometer-sized sized particles offer
novel structural,
ral, optical and electronic properties that
are not attainable with individual molecules or bulk
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 2 | Jan-Feb 2018
S. Lega Priyatharsini
Post Graduate and Research Department
of Botany, Ayya Nadar Janaki Ammal
College, Sivakasi, Tamil Nadu, India
solids (Anitha et al.,
., 2011). The characters of metal
and metal oxide nanoparticles have been of great
interest due to their distinctive feature such as
catalytic activity, optical, magnetic and electrical
properties (Garima et al,, 2010). Nanoparticles
interaction with biological
ical materials and established a
series of nanoparticle / biological interfaces that
depend on colloidal forces as well as dynamic
biophysicochemical interactions. These interactions
lead to the formation of new nanomaterial with
control size shape, surfacee chemistry, roughness and
surface coatings.
The use of plants for the synthesis of nanoparticles is
novel and provides a cost-effective cost and
environmentally friendly alternative to chemical and
physical synthesis. In addition, the use of plants can
be easily scaled up for large--scale synthesis without
the use of toxic chemicals or the need for high
pressures,
sures, energy and temperature (Bhainsa et al.,
2017). Plant-mediated
mediated biosynthesis of zinc oxide
nanoparticles has been achieved in Aloe barbadensis
(Sangeetha et al., 2011), Physalis alkekengi (Qu et al.,
2011), Parthenium hysterophorus (Rajiv et al., 2013),
Zingiber officinale (Bhuyan et al.,
al 2015), Azadirachta
indica (Wang et al., ., 2014), Ocimum basilicum,
Medicago sativa (Nagaiyothi et al., 2013),
Anisochilus carnosus (Bala et al.,
al 2015), milky latex
of Calotropis procera and fruit juice of Citrus
aurantifolia (Samat et al.,
., 2013) etc.
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 International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
It is known that the plant mediated synthesis of ZnO
nanoparticles is much safer and environmentally
friendly as compared to chemical synthesis. The size
of the synthesized ZnO nanoparticles was in the range
of 60-70 nm. The larger nanoparticles of ZnO resulted
from the agglomeration of smaller nanoparticles.
Highly ionic nanoparticulate metal oxides such as
zinc oxide nanoparticles are unique in that they can be
produced with high surface areas and with unusual
crystal structures (Anandraj et al., 2017). Compared
to organic materials, inorganic materials such as ZnO
possess superior durability, greater selectivity and
heat resistance (Padmavathy and Vijayaraghavan,
2008).
II. MATERIAL AND METHODS
SYNTHESIS OF ZINC OXIDE
NANOPARTICLES
The onion bulbs were washed with sterile distilled
water and the outer covering of the bulb was manually
peeled off and the fleshy part of the onion was
rewashed with sterile distilled water. The onion bulb
was cut into small pieces and 10g of bulb was ground
using mortar and pestle with distilled water. The
extraction was filtered using muslin cloth and then
Whatmann No.1 filter paper and used as reducing
agent and stabilizer. Zinc nitrate was used as
precursor for the synthesis of zinc oxide
nanoparticles. For ZnO NPs synthesis, the reaction
mixture was prepared by mixing of 10 ml of leaf
extract and 90 ml of 1mM zinc nitrate solution in a
250 ml Erlenmeyer conical flask. This mixture was
incubated in dark conditions at 37°C. Then, the
mixture was centrifuged at 10,000 rpm for 20
minutes. The pellet was taken after centrifugation and
air dried. The pellet was used for further studies.
RECOVERY OF ZnO NANOPARTICLES
The nanoparticles thus obtained were purified by
repeated centrifugation at 10000 rpm at 25°C for
10minutes. It was followed by re-dispersion of the
pellet in deionized water to get rid of any
uncoordinated biological molecules. The process of
centrifugation and re-dispersion were repeated with
sterile distilled water to ensure better separation of
free entities from the nanoparticles.
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 2 | Jan-Feb 2018
CHARACTERIZATION OF ZnO
NANOPARTICLES
The synthesized nanoparticles were characterized by
UV-Visible spectroscopy, Scanning Electron
Microscopy (SEM), Energy Dispersive X-ray
Spectroscopy Analysis (EDAX), Fourier Transform
Infrared Spectroscopy (FTIR), X-Ray Diffraction
(XRD) and Zeta potential analyses.
UV-VISIBLE SPECTROSCOPY ANALYSIS
The reduction of pure metal ions was confirmed by
measuring the absorption of reaction mixture by UV-
Vis Spectrophotometer (UV 1700 SHIMADZU) from
200 to 800 nm (Roy et al., 2013).
FOURIER TRANSFORM INFRARED (FTIR)
ANALYSIS
FTIR has become an important tool for understanding
the involvement of functional groups in interactions
between metal particles and biomolecules. FT-IR
spectra were recorded at 1 cm-1 resolution by FTIR
spectrophotometer (FTIR-8400S SHIMADZU) using
KBr pellet technique (Prathra et al., 2011). The
frequency range was measured as wave numbers
typically over the range 4000-400 cm-1.
X-RAY DIFFRACTION (XRD) ANALYSIS
To determine the nature and size of the ZnO
nanoparticles, X-ray diffraction (XRD) was
performed. The pellet was dissolved in deionized
sterile water and washed thrice in the same by
centrifugation. The pellet was retained and air dried.
The powder from of the sample was coated on the
XRD grid, the spectra were recorded 40 kV and a
current of 30 mA with CuKα radiation using XRD
(Philips PW1050/37 model). The diffracted intensities
were recorded from 20°C to 80°at 2θ angles. The
crystalline nature of synthesized nanoparticles was
calculated from the width of the XRD peaks, using the
Debye-Scherrer formula (D= Kλ/β cosθ).
SCANNING ELECTRON MICROSCOPY (SEM)
ANALYSIS
The synthesized nanoparticles were dispersed in water
and the resultant suspensions were homogenized
using ultra sonicator for one to two hours. A drop of
the nanoparticles suspension was placed on a piece of
micro glass slide attached to a metal grid coated with
carbon film and dried it gradually at room
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 International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
temperature. The sample was then sputter coated with
gold and visualized with a JEOL JSM-6480 LV SEM
to assess the particle size, shape and percentage
(Forough and Farhadi, 2010).
ENERGY DISPERSIVE X-RAY
SPECTROSCOPY (EDAX) ANALYSIS
A drop of the nanoparticles suspension was placed on
a piece of microglass slide attached to a metal grid
coated with carbon film, and dried it gradually at
room temperature. X-ray spectrometer (EDAX)
operated at an accelerating voltage at 10 KeV. The
sample was then sputter coated with gold and
visualized with a BRUKER to assess the particle size,
shape and percentage of synthesized particles.
ZETA POTENTIAL ANALYSIS
The supernatant was filtered and then sonicated for 5
minutes. The solution was centrifuged for 15 minutes
at 25°C with 5000 rpm and the supernatant was
collected. Then, the supernatant was diluted for 4 to 5
times and then set for Zeta-Potential analysis
(Mohammed et al., 2014).
ANTIMICROBIAL ACTIVITY
The ZnO nanoparticles were tested against the
bacterial pathogens such as E.coli (ATCC25922),
Staphylococcus aureus (ATCC9144), Pseudomonas
aeruginosa (ATCC25619) and fungal pathogens
Aspergillus niger (MTCC 1344) and Aspergillus
flavus (MTCC 1973). Agar disc diffusion method
(Baur et al., 2012) was employed for the study of
antimicrobial activity of the synthesized zinc oxide
nanoparticles.
STATISTICAL ANALYSIS
The XRD values were inferred through JCPDS file no
89-3722. Further, the UV-Visible spectrum, FTIR,
XRD values were interpreted by using ORIGIN
VERSION-8 (data analysis and graphing work space).
The data were reported as mean ± SE and in the figure
parentheses represent the per cent activity.
III. RESULTS & DISCUSSION
VISUAL OBSERVATION OF ZINC OXIDE
NANOPARTICLES
The synthesized zinc oxide nanoparticles were
initially confirmed by visual observation by colour
change in the reaction medium. The metal ions were
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 2 | Jan-Feb 2018
reduced during the exposure to aqueous extract of
onion within 24 hours of incubation period. It was
observed that the colour of the reaction medium was
changed to pale yellow to pale brown colour (Fig. 1).
UV-VISIBLE SPECTROSCOPY ANALYSIS
The reduction of zinc oxide ions in the onion extract
was further confirmed by UV-Vis Spectrophotometer.
UV-Vis absorption spectra of nanoparticles were
shown in (Fig. 2). The UV-Vis absorption spectrum of
ZnO NPs was as the peak maxima in 354 nm which
corresponding to the absorbance of zinc oxide
nanoparticles. After incubation period, the colour was
changed because of the excitation of Surface Plasmon
Vibrations in the ZnO nanoparticle. The reduction of
zinc was subjected to analysis by using the UV-Vis
Spectrophotometer. Absorption spectra of ZnO NPs
formed in the reaction media has absorbance peak at
310nm, broadening of peak indicated that the particles
are polydispersed. The frequency and width of the
surface plasmon absorption depends on the size and
shape of the metal nanoparticles as well as on the
dielectric constant of the metal itself and the
surrounding medium (Melvin et al., 2009).
The aqueous extract of Punica granatum and zinc
nitrate solution, the colour of the reaction medium
changed rapidly from colour less to brownish yellow.
The colour indicated surface plasmon vibration
typical of zinc oxide (ZnO) nanoparticles. The
maximum intensity at 364nm was observed indicating
complete reduction of zinc ions. The UV-Vis optical
absorption spectrum of ZnO nanoparticles showed a
sharp absorbance at 345 nm, which indicated an
almost uniform size of the nanoparticles. However,
upon change in particle size or particle shape, a slight
shift in the absorption was observed (Guo et al.,
2001). The UV-Vis absorption spectrum of ZnO
nanoparticles using flower extract of Nyctanthes
arbor-tristis showed a sharp absorbance at 369 nm
(Jamdagni et al., 2016).
FOURIER TRANSFORM INFRARED
SPECTROSCOPY (FTIR) ANALYSIS
FTIR analysis was carried out to identify the
biomolecules which were responsible for the
reduction of metal ions into ZnO nanoparticles in the
presence of onion extract (Fig. 3). The phytochemical
found in the onion extract were responsible for the
formation of various nanoparticles. The FT-IR
spectrum of onion extract showed several absorption
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 International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
peaks ranged from 3421cm-1 to 677 cm-1. The region
of band was phenols, alkanes due to N-H stretching of
proteins and O-H stretching, > C=O stretching of
esters, aromatics, ring C-C stretching of phenyl,
alkanes, C-O stretch in vibration combined with the
ring stretch of phenyl, aliphatic amines, alcohols,
carboxylic acids, ester, ether, functional groups
mainly from carbohydrate and alkyl halides. FTIR
measurements of zinc oxide nanoparticles with onion
extract showed the presence of bands at 3438.84,
2925.81, 1633.59, 1383.83, 1122.49, 1104.17 and
600.78 cm-1. These bands were indicting the presence
of N-H bend primary amines, C-O stretching alcohols,
carboxylic acids, ester and ethers.
FTIR spectrum of ZnO nanoparticles revealed that the
peak at 417.52cm-1 was the characteristic absorption
of ZnO bond and the broad absorption peak at
3438cm-1 attributed to the characteristic absorption of
hydroxyl group (Khan et al., 2011). The FTIR
spectrums of ZnO NPs derived from Corymbia
citriodora leaf extract showed the strong absorption
peaks observed at 3300 and 1620cm−1 and were
assigned to O–H stretch and N–H bend functional
groups. Weaker bands observed at 2955, 777 and
633cm−1 were assigned to –C–H stretch (alkanes), C–
H (aromatics) and –C=C–H (alkynes). The absorption
peaks at 1520 and 1431cm−1 were attributed the C=C
bending and C–C stretching of aromatic rings,
respectively. The peak at 1053 cm−1 assigned to the
C–N stretching mode in aliphatic amines. The
formation of ZnO NPs may attribute to the chemicals
presence in the C. citriodora such as citronellal,
linalool, catechin, gallic acid, courmaric acid and
protocatechuric acid, which acted as reducing agents
as well as stabilizing agents (Tamuly et al., 2013).
The FTIR spectrum of ZnO nanoparticles derived
from Ocimum tenuiflorum showed the fundamental
mode of vibration at 3458.04cm−1 which
corresponded to the O-H stretching vibration,
1625.35cm−1 which correspond to the N-H bend,
1418.86cm−1 which corresponded to C-C stretching
vibration of alcohol, carboxlic acid, ether and ester
were confirmed. The band at 1148.10cm−1
corresponds to C-N symmetric stretching vibration
and O-H bending of the hydroxyl group at
3458.04cm−1 was observed. The absorption at
875.23cm−1 was due to the formation of tetrahedral
coordination of Zn. The bond at 835.61cm−1 was due
to the C-Cl stretching vibration. The peak at
668.29cm−1 indicated the stretching vibrations of ZnO
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 2 | Jan-Feb 2018
nano particle which was consistent with that reported
before (Ravichandrika et al., 2012). From FTIR
analysis of leaves extract of Ocimum tenuiflorum all
functional groups play important role in the
preparation of zinc oxide nanoparticles.
Green synthesis of ZnO nanoparticles using
Phyllanthus embilica stem extract revealed The FT-IR
spectra showed the presence of bonds due to O-H
stretching around 3423 cm-1. Peak at 1405 cm-1 may
be assigned to symmetric stretching of the carbonyl
side groups in the amino acid residues of the protein
molecules. The band at 1022 cm-1 was corresponding
to C-N stretching vibration of amine. The peak at
around 1340 cm-1 present in green ZnO signified
amide III band of the random coil of protein (Joel and
Sheik, 2017).
X-RAY DIFFRACTION PATTERN ANALYSIS
The various peaks in the XRD pattern could be
assigned to the crystalline zinc oxide phase with the
hexagonal wurzite structure with the lattice
parameters a=3.2475 nm and c= 3.2501 nm. The XRD
pattern showed different intensity peaks in the whole
spectrum of 2θ values ranging from 20° to 80° for the
onion. The Allium cepa extract-mediated synthesized
ZnO nanoparticles were indexed as (100), (002),
(101), (102), (110), (103), (200), (112) and (201). The
zinc was indexed as (100) and (101). The average
crystalline size was determined using Scherrer’s
equation. The average crystalline size of zinc oxide
nanoparticles was 20-100 nm (Fig. 4). Zinc oxide
nanoparticles synthesized using Corymbia citriodora
leaf extract showed the peaks at 32.1°, 34.6°, 36.1°,
47.7°, 56.4°, 63.1° and 68.1° and indexed to
hexagonal wurtzite ZnO (JCPDS 36–1451). Further,
no other peaks have been detected in the sample and
confirmed the biosynthesis of ZnO NPs owning a high
purity. The mean crystalline size was found to be 21
nm (Yuhong Zheng et al., 2017). Biological synthesis
of zinc oxide nanoparticles from Catharanthus roseus
leaf extract showed strong diffraction peaks at 20, 32,
35 and 40 degrees of 2q which corresponds to 111,
200, 220 and 311 crystal planes, which were
significant agreement with the JCPDS file 36145 (a =
b = 3.249A0, C = 5.206 A0) and indexed as the
hexagonal wurtezite structure of ZnO. High purity and
crystallinity of the synthesized ZnO NPs were also
confirmed.
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 International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
SCANNING ELECTRON MICROSCOPE (SEM)
ANALYSIS
The surface morphology of the nanoparticles was
characterized using Scanning Electron Microscopy.
The onion extract mediated ZnO nanoparticles were
found as nano-rod shape (Fig. 5). The biological
approach using milky latex of Calotropis procera has
been used for the first time as a reducing material as
well as surface stabilizing agent for the synthesis of
spherical-shaped ZnO NPs. The structure, phase and
morphology of synthesized product were investigated
by the SEM analysis (Sangeetha et al., 2011). Raut et
al. (2017) done the SEM analysis for zinc oxide
nanoparticles synthesized by Ocimum tenuiflorum leaf
extract. The SEM analysis showed the hexagonal
nanoparticle formed with diameter range 11-25nm.
ZnO nanoparticles synthesized using Trifolium
pratense flower extract revealed that SEM images of
the ZnO nanoparticles were agglomerated with a
particle size ranging from 100–190nm (Dobrucka and
Dugaszewska, 2015).
EDAX ANALYSIS
EDAX analysis is very much useful for further
confirmation of presence of nanoparticles. The
EDAX analysis showed the confirmative peaks for
ZnO NPs. The ZnO nanoparticles were confirmed by
typical absorption peak at 1KeV (Fig. 6). Sharma et
al. (2014) reported that the size of the nanoparticle is
more than that calculated from the Debye -Scherrer
formula indicating the agglomeration of crystallites in
ZnO nanoparticles. The aggregation of particles
should have been originated from the large specific
surface area and high surface energy of ZnO
nanoparticles. The aggregation occurred probably
during the process of drying (Raoufi, 2013). The
green synthesis of zinc oxide nanoparticles using
Moringa oleifera leaf extract the strong and weak
peaks are observed from Zn and O atom and weak
peaks are observed form S, K, C, P, Ca element
(Mishra et al., 2015). Biosynthesis and
characterization of ZnO nanoparticles using the
aqueous leaf extract of Imperata cylindrica the EDS
spectrum shows the high value of zinc (80.3%) and
oxygen (19.65%).
ZETA POTENTIAL ANALYSIS
Zeta potential measures the potential stability of the
nanoparticles in the colloidal suspension. From the
Zeta potential analysis, onion extract mediated ZnO
nanoparticles carried a charge of ±26.5 mV (Fig. 7).
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 2 | Jan-Feb 2018
The zeta potential of the biologically synthesized NPs
was determined in water as dispersant. The zeta
potential was found to be -20 to -30 mV for ZnO
nanoparticles. The high negative value approved the
repulsion among the particles and thereby increases in
stability of the structure of nanoparticles (Jafarirad et
al., 2016). It was suggested that the surface of the
nanoparticles was negatively charged and dispersed in
the medium. The negative value confirms the
repulsion among the particles and proves that they are
very stable (Anandalakshmi et al., 2016). The
biosynthesis of zinc oxide nanoparticles using Ixora
coccinea leaf extract zeta potential was found to be
−49.19 mV (Yedurkar et al., 2016). The synthesis and
characterisation of zinc oxide nanoparticles using
terpenoid fractions of Andrographis paniculata leaves
the zeta potential of synthesized ZnO nanoparticles
was 17.6 mV.
ANTIMICROBIAL ACTIVITY
Antimicrobial activity of onion extract mediated zinc
oxide nanoparticles were tested against bacterial
pathogens such as E.coli and Staphylococcus aureus,
Pseudomonas aeruginosa and two fungal pathogens
like Aspergillus niger and Aspergillus flavus. The
antibacterial activity (inhibition zone) of zinc oxide
nanoparticles was higher against Pseudomonas
aeruginosa followed by Staphylococcus aureus and
E.coli. In the case of fungal pathogens, the maximum
inhibition zone was observed against A. niger (Table
1).
Nanoparticles possess the antimicrobial properties
against several microorganisms including bacteria,
fungi and certain extremophiles (Haeed et al., 2008).
The properties of ZnO nanoparticle is strongly
influenced by the particle size and mechanism of cell
inhibition. The properties include disruption of cell
membrane, altering the permeability, electrostatically
binding to the cell surface and accumulation of
nanoparticle in cytoplasm (Stoimenov et al., 2002).
Antimicrobial activity of zinc oxide nanoparticles
were known from the very distant past and has many
applications in disinfecting medical devices, water
purification, and wound healing, creams, lotions and
antibacterial creams. The mechanism of action of
antimicrobial activity zinc oxide is similar to other
nanoparticles, but it acts mostly through destruction of
bacterial walls. Zinc oxide nanoparticles have been
widely used against Gram-positive and Gram negative
bacteria (Emami et al., 2013).
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 International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
The mechanisms of antibacterial activity of ZnO NPs
were direct contact of ZnO NPs with cell walls,
resulting in destructing bacterial cell integrity,
liberation of antimicrobial ions mainly Zn+2 ions.
However, the toxicity mechanism varies in various
media as the species of dissolved Zn may change
according to the medium components besides the
physicochemical properties of ZnO NPs (Yedurkar et
al., 2016). Nano-sized ZnO exhibited varying
morphologies and showed a significant antibacterial
activity over a wide spectrum of bacterial species
explored by a large body of researchers. ZnO is
currently being investigated as an antibacterial agent
in both microscale and nanoscale formulations. ZnO
exhibited significant antimicrobial activities when
particle size is reduced to the nanometer range, then
nano-sized ZnO interacted with bacterial surface
and/or with the bacterial core where it entered inside
the cell and subsequently exhibited distinct
bactericidal mechanisms (Seil and Webster, 2012).
CONCLUSIONS
The biological production of metal nanoparticles is
becoming a very important field in chemistry, biology
and materials science. Metal nanoparticles have been
produced chemically and physically for a long time;
however, their biological production has only been
investigated very recently. The biological reduction of
metals by plant extracts has been known since the
early 1900s; however, the reduction products were not
studied. The rapid biological synthesis of zinc
nanoparticles using bulb extract of Allium cepa
provides an environmental friendly, simple and
efficient route for synthesis of nanoparticles. The use
of plant extracts avoids the usage of harmful and toxic
reducing and stabilizing agents. The synthesized nano
particles of ZnO are in the range of 20-100 nm. Zinc
nanoparticles can exist in ions only in the presence of
strong oxidizing substances. The synthesis of ZnO
nano particles is still in its infancy and more research
needs to be focused on the mechanism of nanoparticle
formation which may lead to fine tuning of the
process ultimately leading to the synthesis of
nanoparticles with a strict control over the size and
shape parameters.
ACKNOWLEDGEMENT
The authors are thankful to the Management and the
Principal of Ayya Nadar Janaki Ammal College,
Sivakasi, Tamil Nadu, India for providing laboratory
facilities to carry out the experiment.
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 2 | Issue – 2 | Jan-Feb 2018
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Fig. 3: FTIR spectrum of Zinc oxide nanoparticles

Onion Extract ZnNO3 ZnO NPs

Fig. 1: Visual observation of synthesis of Zinc

oxide nanoparticles
Fig. 4: XRD spectrum of the Zinc oxide
nanoparticles

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 Fig. 6: EDAX spectrum of Zinc oxide
nanoparticles

Fig. 7: Zeta potential spectrum of Zinc oxide

Fig. 5: SEM micrograph of Zinc oxide nanoparticles
nanoparticles at different magnifications

Table 1: Antimicrobial activity of Zinc Oxide nanoparticles

Diameter of inhibition zone (mm)

Microorganism
Onion Extract Zinc oxide
nanoparticles
E. coli 0.91 1.9
±0.02 ±0.04
P. aeruginosa 1.72 2.5
±0.03 ±0.04
S. aureus 1.6 2.4
±0.04 ±0.07
A. niger 1.2 2.9
±0.09 ±0.03
A. flavus 1.6 2.2
±0.09 ±0.02