Professional Documents
Culture Documents
Yu Yang1, Jun Yang1, *, Wei-Min Wu2, Jiao Zhao3, Yiling Song4, Longcheng Gao1,
1
Key Laboratory of Bio-Inspired Smart Interfacial Science and Technology of
2
Department of Civil and Environmental Engineering, William & Cloy Codiga
Resource Recovery Research Center, Center for Sustainable Development & Global
3
Shenzhen Key Laboratory of Bioenergy, BGI-Shenzhen, Shenzhen 518083, P. R.
China.
4
School of Biological Science and Medical Engineering, Beihang University, Beijing
100191, P. R. China.
1
Supplementary Figures
Figure S1. Inhibition of the growth of bacteria extracted from the guts of mealworms
in the presence of 6 different antibiotics. (a) Ampicillin with strong halo of bacterial
colony inhibition (1.6 ± 0.2 cm). (b) Chloramphenicol without distinct halo of
bacterial colony inhibition. (c) Erythromycin without distinct halo of bacterial colony
inhibition. (d) Gentamicin with strong halo of bacterial colony inhibition (2.4 ± 0.1
cm). (e) Tetracycline with strong halo of bacterial colony inhibition (2.3 ± 0.1 cm). (f)
Vancomycine without distinct halo of bacterial colony inhibition. The central disk in
each plate was the purified water control and the surrounding disks were added
respective antibiotic at dosage of 30 µg per disk. Based on above results, we selected
gentamicin as the antibiotic to feed the mealworm larvae in order to eliminate gut
bacteria.
2
Figure S2. Change in bacterial cell number colonized on the PS film samples
incubated with thirteen different isolated bacterial cultures after 28 days. The strain
YT2 showed the best performance among all isolates.
3
Figure S3. 16S RNA gene-based Neighbor-joining phylogenetic tree of strain YT2.
The numbers above the branches are support value obtained from 1,000 bootstrap
replicates. Percentages of 16S RNA gene nucleotide sequence identities between
strain YT2 and each of the comparison species are shown in parentheses. The results
indicated that the isolated strain YT2 belongs to Exiguobacterium genus.
4
Figure S4. Gas chromatograph of extract from the culture inoculated with strain YT2
and the uninoculated control after 60 days.
5
Supplementary Tables
6
Table S2. GC/MS identification of water soluble products released from PS pieces in
the presence and absence of the activities of strain YT2 after 60 days. (Control=in the
absence of strain YT2)