MAPIMS

Laboratory Safety Manual

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 Laboratory Safety Manual
Contents

S.No Topic Page No

1 Introduction- Safety management system 3

2 Universal safety precautions 6

3 Safety Rules & Practices for Lab working areas 8

4 Safety Rules & Practices for Personal protection 9

5 Biowaste disposal as per BMW rules 2016 10

6 Material safety data sheet - MSDS 11

7 Safety management for Biological spill 14

8 Safety management for occupational exposure to HBV & HIV 15

9 Safety management for Chemical & Fire accident 16

10 Laboratory sample transport 17

11 Decontamination 19

12 Safety Documents 21

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 1. Introduction- Safety Management system

The purpose of implementation of this system as a part of quality assurance program is

 To ensure safety of laboratory employees, Equipment, Patients and environment,

 To retain the reputation , customers,employees
 To reduce cost effect.

Responsibility

It is the responsibility of each & every staff of laboratory

1.All should know the basic safety rules & safety practices

2.All should understand the basics of safety,potential laboratory hazards & and safety management

3.All should know the proper handling of blood and other potentially hazardous pathological materials

4.All should know the decontamination technique and disposal of infectious material.

Materials required

 Safety manual – It includes essential elements for laboratory design ,Introduction about lab
hazards & safety, General safety rules & practices to be followed, Guidelines of biowaste
disposal,MSDS (Material safety data sheet), First aid Safety management for physical, chemical
& Biological hazards.

 Document

1. Personal risk(accident) Notification form

2. Biological risk assessment -Central lab-- 1.Patients culture report statistics

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 2. Lab culture report

3. Bioprophylaxis of Lab staff for HBV

4. Cleaning Protocol format for central lab

Process

1. Periodical education of lab staff regarding safety precautions

2. Notification of accidents

3. Supervision of lab cleaning process , safety practices & waste disposal

4. Recording of vaccine detail of employees & culture reports to identify biological risk.

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Laboratory design- features and facilities for safety

1. Ample space must be provided for the safe conduct of laboratory work and for
cleaning and maintenance.
2. Walls, ceilings and floors should be smooth, easy to clean, impermeable to liquids
and resistant to the chemicals and disinfectants normally used in the laboratory.
Floors should be slip-resistant.
3. Bench tops should be impermeable to water and resistant to disinfectants, acids,
alkalis, organic solvents .
4. Illumination should be adequate for all activities. Undesirable reflections and glare
should be avoided.
5. Storage space must be adequate to hold supplies for immediate use. Additional long-term storage
space, conveniently located outside the laboratory working areas, should also be provided.
6. Facilities for eating and drinking and for rest should be provided outside the
laboratory working areas.
7. Hand-washing basins, with running water if possible, should be provided in each
laboratory room, preferably near the exit door.
8 Autoclave or other means of decontamination should be available in appropriate proximity to the
laboratory.
9. First-aid areas or rooms suitably equipped and readily accessible should be available
10. Sample path & Patient path should not be cross linked
11.Sample collection area is better located near reception that saves time & energy
12.Sample processing area ( Centrifugation,& distribution to appropriate sections of lab) should be
separated from,but nearby the testing areas.
13.Circulation pathways of biological sample inside the laboratory & contaminated wastes should be
separated to prevent contamination.

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Laboratory hazards

All laboratory staff should be aware of the risks , potential physical , chemical and biological hazards
inside the laboratory premises.
Physical hazards – Needle prick injury,cut injury by broken glasses,sharps,accidental slip & fall,thermal
injury from hot air oven,incubator,pressure & mechanical injury from centrifuge,electrical injury,fire
accident.
Chemical hazards – Chemical Burns ,accidental ingestion,splash over face,Inhalation of toxic gaseous
chemicals
Biological hazards –Acquiring infections (especially dreaded infections like HIV,HBV,HCV)through
inhalation,ingestion & percutaneous mode of spread from the infected biological samples like
blood,semen,CSF and other body fluids..

1. Inhalation risks (i.e. aerosol production) when using loops, streaking agar plates,
pipetting, making smears, opening cultures, taking blood/serum samples,centrifuging, etc.
2. Ingestion risks when handling specimens, smears and cultures.
3. Risks of percutaneous exposures when using syringes and needles.

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 2. Universal Safety Precautions ( USP)
1.What is USP?

It is the practice followed in medicine to avoid contact with blood,body fluids by means of wearing
protective non porous barrier like glove,mask,face shield,Apron,Goggles and hand wash.

2.Why it is called as Universal?

All patients should be considered as though they are having potential blood borne pathogens & can
infect any health care worker.

3.What for USP has to be followed?

Any unsafe contact can spread or pass on the infectious agent that lead to dangerous diseases (
HBV,HCV,HIV).So in order to protect workers from exposure to diseases spread by blood & body fluids
,protect the environment as well as to prevent spread from one to other patient, it has to be followed.

Microbial source ----- Transmission ------Susceptible host ------Nosocomial infection.

4.What are high risk & low risk body fluids?

High risk – Blood,semen,vaginal secretions,amniotic fluid,synovial fluid,body cavity fluid

Low risk – Tears,nasal secretions,saliva,sputum,vomitus,urine,stool. Though it may contain

microorganism, they are not considered as blood borne pathogen & not cause dangerous disease.

5.What are the Modes of transmission of disease?

Percutaneous inoculation, Ingestion by contaminated hands, Inhalation by aerosol & droplet, Surface
absorption.

6.What are the practices included in USP?

 USP has to be applied universally for all patients

 Hand wash –Nothing is superior to hand wash in preventing spread.
 Never touch the skin.Wear PPE –Personal protective equipment like glove,mask,goggles,face
shield,apron,foot wear
 Use disposable syringe & needle carefully & avoid recapping.If necessary recapping to be done
by one hand technique.
 Decontamination of equipment
 Proper cleaning of spill by using 1-2% Sodium hypochlorite solution. Minimum contact period is
20 minutes. Then mop with soap solution.
 There should be a system for proper waste collection ,segregation & disposal

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7..Describe Hand wash technique?

It is the basic level of infection control precautions.

 Indications
- Prolonged contact with patient

-Before & after handling of wounds

-Before taking care of Immunocompromised patients,ICU ,burns ,pediatrics patients

-After contact with body fluids,mucous membrane

 What to be used for hand wash – Detergent soap,alcohol, Antiseptics like chlorhexidine
 Duration – one minute is sufficient
 Technique – It involves 12 steps of rubbing.
1.Take materials (soap) in hand. Rub the palm on both sides (2)
2.Rub dorsum of hand both sides (2)
3.Rub interdigital areas of both hands (2)
4.Rub by interlocking the fingers on both sides (2)
5.Rub the thump fully on both sides(2)
6.Rub the finger tips by rotating movement of all tips over palm on both sides (2)

 8.Why waste has to be segregated before disposal?

1. To prevent contamination of predominant non infectious waste with infectious one.
2. To avoid illegal reuse
3. Safe disposal of infected waste by incineration & protect the environment & public.
4. Contaminated Plastics, glasses, metals can be decontaminated & shredded for recycling

9.How waste can be segregated?

 Infected materials like body tissue, body fluids, animal tissue,chemicals,drug

ampoules,microbiological waste – To be discarded in yellow color coded bag that has to be
incinerated.
 Contaminated plastics like IV tube,bottle,catheter,urobag –To be discarded in red color coded
bag that has to be autoclaved then shredded & recycled.
 Contaminated as well as non contaminated glasses,glass tubes,broken pieces to be collected in
cardboard box with blue label that has to be autoclaved then shredded & recycled.
 Contaminated needles,metals,instruments,blades, or any sharps to be discarded in puncture
proof container that has to be autoclaved then shredded & recycled.

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 3. Safety Rules & Practices for Lab working areas

Basic Safety Rules – For Laboratory working areas

General Safety 1.PPE- Personal protective equipment – Glove,mask,face shield,Goggles,Apron

Equipment
2.Fire extinguishers & Blankets
3.Appropriate storage & cabinets for flammable & toxic chemicals
4.Waste disposal equipment
5.First aid equipment, Eye washers & shower
6.Spillage kit – PPE, Hypo, cotton cloth,Disposable yellow coded bag

General Safety 1.Biohazard warning sign for laboratory doors

Practices
2.Laboratory doors should be kept closed.
3.Only authorized persons should be allowed to enter the laboratory working
areas.
4.Children should not be authorized or allowed to enter laboratory working
areas.
5.The laboratory should be kept neat, clean and free of materials that are not
pertinent to the work.
6.Never leave the spillage area unattended.
7. Work surfaces must be decontaminated after any spill of potentially
dangerous material and at the end of the working day.
8. All contaminated materials, specimens and cultures must be decontaminated
before disposal ( Chemical- 2% Hypo) or cleaning for reuse(Autoclave)
9. Packing and transportation must follow applicable national and/or
international regulations.
10.Recording & Maintenance of cleaning & disinfection of laboratory areas –
Daily,weekly,monthly basis
11.Biosafety cabinet should be used to prevent aerosol spread while handling
large volumes of infectious agents.
12.Biological , chemical & general wastes to be segregated & disposed in
appropriate container.

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 4. Safety Rules & Practices for Personal protection

Basic Safety Rules – For Personal protection

DO”s DON”Ts inside lab premises

1.Aware of lab hazards & safety measures 1.Eating, drinking, smoking, applying cosmetics
2.Laboratory coat & uniform must be worn at all and handling contact lenses is prohibited in the
times for work in the laboratory. laboratory working areas.
3. Appropriate gloves must be worn during all
2.Storing human foods or drinks anywhere in the
procedures of handling blood, body fluids and other
laboratory working areas is prohibited.
potentially infectious materials
3.Mouth pipetting is prohibited
4. After use, gloves should be removed and hands
4.Prohibiting sandals and open toed shoes to be
must then be washed.
worn inside the laboratory working area.
5. Personnel must wash their hands after handling
5.Avoid smelling & tasting chemicals.
infectious materials & before they leave the
6.Never recap needles.
laboratory working areas.
7.Never open the centrifuge before it stops
6. Safety glasses, face shields (visors) or other
completely.
protective devices must be worn when
8.Do not discard samples directly into the sink.
it is necessary to protect the eyes and face from
9.Do not use phone & computer with
splashes.
contaminated gloved hands
7.Proper disposal of biological & chemical waste in
10.Do not use laboratory glassware to consume
appropriate container.
food & water.
8.Know the location of safety equipment such as fire
extinguisher,spillage kit,first aid kit and telephone
numbers.
9.Labelling & proper storage of chemicals.
10.Restrain & tie up long hair to avoid accident.

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 5.Biowaste disposal as per BMW rules

Category Type of waste Type of bag or Treatment & Disposal

container
used
Yellow Human tissues,body parts Yellow color 1.Incineration (or)
coded bag 2.PlasmaPyrolysis (or)
3.Deep burial
Animal tissues 1.Incineration (or)
2.PlasmaPyrolysis (or)
3.Deep burial
Soiled wastes- cotton ,pad soiled with 1.Incineration (or)
body fluids,blood, bag with residual 2.PlasmaPyrolysis (or)
blood
Expired medicines 1.Incineration (or)
2.PlasmaPyrolysis (or)
Chemical waste 1.Incineration (or)
2.PlasmaPyrolysis (or)
Chemical liquid waste Pretreatment with Hypo

Discarded linen, mattress Pretreatment with Hypo

contaminated with body secretions Followed by Incineration

Microbiological & clinical laboratory Pretreatment with Hypo

waste including cultures Followed by Incineration
Red Contaminated plastic waste Red color Autoclaving followed by
( Recyclable) coded bag shredding
Disposable glove,syringe without Plastic waste should not be sent
needle,tube,IV bottles,catheter,urobag to landfill sites.
Blue Broken glass, medicine Cardboard Pretreatment & sent for recycling
ampoules,Metalic body implants box with blue
label
White Needles,sharp blade,scalpel,sharp Puncture Autoclaving or Dry Heat
metals proof Sterilization followed by
container shredding - Recycling

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 6.Material safety data sheet - MSDS

1. MSDS -- Hematology

A.5 PART CELL COUNTER

Regent name:
1. 5 Part M52 Diluents, 2 . 5 Part Rinse, 3. 5 Part LH Lyse 4. 5 Part DIFF lyse, 5. 5Part Cleanser

Characteristics -- Irritation

Storage recommendations Health hazards First aid

15 to 35*C Avoid contact with eyes and skin In case of contact rinse immediately
Precautions & PPE of water and seek medical advice
Wear glove and mask

B.Regent name:
1.Leishman Stain 500 ml Characteristics Flammable

Storage recommendations Health hazards First aid

+4*c to +40*c Avoid contact with eyes and skin
Precautions & PPE In case of contact rinse immediately
Wear glove and mask of water and seek medical advice

C.Regent name:
1.WBC Diluting fluid,2.RBC Diluting fluid,3.Eosinophil Diluting fluid, 4.Reticulocyte Counting Fluid
5.Platelet Counting Fluid,6.N/10 HCL

Characteristics - Irritation

Storage recommendations Health hazards First aid

Room Temperature Avoid contact with eyes and skin In case of contact rinse immediately
Precautions & PPE of water and seek medical advice
Wear glove and mask

D.Regent name:
1.HB Reagent(Potassium Ferric Cyanide)
Characteristics - Poisonous

Storage recommendations Health hazards First aid

Room Temperature Do not Mouth Pipette it since it is In case of contact rinse immediately
Poisonous, & avoid contact with of water and seek medical advice
eyes and skin.
Precautions & PPE
Wear glove and mask

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2.MSDS – Clinical Pathology

A.Regent name:
1.Sodium Hypochlorite 4%
2.Barium Chloride solution 10%
3.Sulphosalicylic Acid solution 3% 4.Semen Diluting Fluid 125 ml
5. Rothras Mixture powder 100 g.
6.Sulphur Powder 100 g
7.Ferric Ammonium Sulphate
8.Sodium Dihydrogen Phosphate
9.Resorcinol

Characteristics - Irritation

Storage recommendations Health hazards First aid

15* to 35*C Avoid contact with eyes and skin In case of contact rinse immediately
Precautions & PPE of water and seek medical advice
Wear glove and mask

B.Regent name:
1.Ammonia Solution

Characteristics- Irritation & toxic

Storage recommendations Health hazards First aid

Cool to below 20*c Avoid contact with eyes and skin In case of contact rinse immediately
Precautions & PPE of water and seek medical advice
Wear glove and mask

C.Regent name:
1. Ether
Characteristics- Flammable

Storage recommendations Health hazards First aid

Room Temperature Avoid contact with eyes and skin In case of contact rinse immediately
Precautions & PPE of water and seek medical advice
Wear glove and mask

D.Regent name:
1.Benedict Reagent
Characteristics - Irritation

Storage recommendations Health hazards First aid

Room Temperature Avoid contact with eyes and skin. In case of contact rinse immediately
Precautions & PPE of water and seek medical advice

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 Wear glove and mask

3.MSDS – Biochemistry

A.BIO SYSTEM Fully Auto Analyser

Reagent name:
1. Systamic Solution, 2.Multiclean Solution,3. Hypo Chloride.

B.Semi Auto Analyser:

Reagent name:
Bio system Cleaning solution.

Characteristics -- Irritation

Storage recommendations Health hazards First aid

15* - 30*C Avoid contact with eyes and skin In case of contact rinse immediately
Precautions & PPE of water and seek medical advice
Wear glove and mask

C.Reagent name:
1.Glucose,2.Urea,3.Cholesterol,4.Triglycerides,5.HDL Cholesterol,6.CK Nac, 7.CK MB, 8. Total Protein, 9.SGOT,
10.SGPT,11.AlkalinePhosphatase,12.GammaGT,13.Amylase,14.Lipase,15.LDH,16.UricAcid,17.Microprotein,
18.Cholinesterase.
1. Characteristics: Irritation

Storage recommendations Health hazards First aid

2* – 8 *C Avoid contact with eyes and skin
Precautions & PPE In case of contact rinse immediately
Wear glove and mask of water and seek medical advice

D.Regent name:
1.Albumin,2.Bilirubin,3.Creatinine,4.TotalCalcium,5.Phosphorus

Characteristics - Irritation

Storage recommendations Health hazards First aid

Room Temperature at 15* – 30 *C Avoid contact with eyes and skin In case of contact rinse immediately
Precautions & PPE of water and seek medical advice
Wear glove and mask

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 7. Safety management for Biological spill
Name of the protocol
1.Biological spill – Blood,
Body fluids
Safety management ( First aid) for Biological spill or accident
Persons 1.Clean the exposed part. – soap water for skin, water flush for
eye, saline for mouth
2.Notify supervisor, & Authorities
3.Apply First aid & treat as emergency
4.Follow appropriate reporting procedures
5.Report to Physician for treatment or counseling.
Place 1.Define the contaminated area & Alert coworkers
2.Wear appropriate PPE & remove glasses by forceps
3.Apply absorbent towels to the spill & Disinfectant (1-2% Hypo
solution ) to the towel surface
4. Leave it for 20 minutes.
5.Remove the towel, Mop it up with soap water solution
6. Properly dispose all materials in yellow coded bag.
7.Notify Authorities
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 8. Safety management for occupational exposure to HBV & HIV
Name of the protocol
Prophylaxis
Accidental exposure to
infected blood through needle
prick or splash into eyes or
mouth
Procedure
Safety management for occupational exposure to HBV,HCV,HIV
1.All lab staff should have HBV vaccine -3 completed doses
2. All lab staff should follow universal personal safety precautions
3.All lab staff should follow safety procedures as described in lab safety manual
Step -1. First aid
1.Wash the site with soap & water
2.Allow the wound to bleed freely
3. Do not put dressing over the wound
4.Wash mouth,eyes,face ,affected site with water for 10 minutes.(In case of
splash).
Step-2. Risk Assessment
High risk – More volume,wide bore needle,deep penetration,type of fluid like
blood
Low risk – Less volume, surface spill with intact skin, type of fluid like CSF
Step -3. Evaluate the source of exposure
Test the source person with his/her consent
Step -4. Management of individuals exposed to HBV,HIV positive sources.( No
PEP for HCV exposure)
Post exposure prophylaxis of HBV
Depends on Immune status of Individual
1.Unvaccinated – HBV vaccine & HB Immunoglobulin
2.Vaccinated & Anti HBsAg > 100 IU /ml – No prophylaxis
3.Vaccinated & Anti HBsAg < 100 IU /ml – – HBV vaccine booster dose
Post exposure prophylaxis of HIV
1.Councelling
2.Testing for HIV antibodies – baseline,6-12 weeks,6 months
3.Antiretroviral therapy – ARV drugs to be started within 72hours & continued
for 28 days.If source person is negative – Stop prophylaxis.
4. Follow up the patient. Advised to use contraceptives & avoid blood donation
Step -5. Report the incident
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 9. Safety management for Chemical & Fire accident

Name of the protocol Safety management for Chemical & Fire accident
1.Chemical spills Minor spill Persons 1.Inform the In charges
2.Follow the recommendations suggested by
manufacturer
( See MSDS)
Place 1.Clean up the spill using appropriate
absorbant cotton, cloth
2. Neutralize residues and then
decontaminate the area.

Major spill Persons 1.Inform the In charges.

2. Follow the recommendations suggested by
manufacturer
3.Refer to casualty for further management.

Place 1.Alerting other coworkers to move to safe

location.
2.Authorities to be informed
3. Follow the recommendations suggested by
manufacturer

2. Fire accident Small fire – It is extinguishable
within 1-2 minutes
1.Cover the fire with inverted beaker or wet
towels
2.If not controlled, use fire extinguisher.( BC
or ABC type)
“PASS” technique – Pull,Aim, Squeez ,Sweep

Large fire Inform local authorities, fire department,

Police department.
Protect 4”M” & one “E” –Man,Machine,
Material, Money & Environment

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 10. Laboratory sample Transport
Quality of laboratory report as well as biological safety relies on proper sample transport. For this

 All Samples should be packed & tranported carefully to avoid breakage as well as leakage of
the specimen
 All samples should be transported with required appropriate temperature maintenance.

Materials required -Basic Triple package system

1.Primary leak proof container which is a sample collection container with proper labeling.

2.Secondary leak proof container which is a resealable plastic bag to contain any leakage in the event of
damage.

3.Tertiary rigid outer package which is a specimen transport box to protect the specimen from damage if
package is dropped.

4.Pre frozen gel pack, Perforated & unperforated sponge for temperature maintenance.

Guidelines for safe transport

 Ensure container lids are tightly secured.

 Ensure samples are labeled properly.
 Place sample tubes, containers in upright position
 Keep the requisition slip in the bags outside pouch
 Don’t roll requisition slip with specimen or attach with tape,pin or staples
 Enclose ice pack to assist in maintaining temperature.
 Ensure specimen container is not in direct contact with ice so sample does not freeze.
 Transport box should be in upright position
 Biohazard symbol should be put over the box.

Guidelines for transport with required temperature maintenance

Some laboratory parameters, analytes need particular temperature maintenance for stability & report
accuracy. According to that Samples are transported either in frozen state , or in refrigerated
temperature 2-8C , or in temperature of 18-22C or at room temperature.

For maintaining temperature , pre frozen gel pack is used which should be frozen 24 hours prior to use.

For Frozen temperature

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  Take empty thermocol box
 Place perforated sponge at the bottom of the box.
 Place frozen gel pack over the sponge & specimen sealed in zip lock bag over the gel pack .
 Place another frozen gel pack over it & cover with second layer of perforated sponge.
 Then keep unperforated sponge over it & close the lid.
 Seal it & transport immediately. Indicate frozen sample on cardboard box.

For Refrigerated temperature – 2-8 C

 Take empty thermocol box

 Place frozen gel pack at the bottom of the box.
 Place perforated sponge over the gel pack & specimen sealed in zip lock bag over the sponge .
 Place second layer of perforated sponge over the sample and another frozen gel pack over it .
 Then keep unperforated sponge over it & close the lid.
 Seal it & transport immediately. Indicate Refrigerated sample on cardboard box.

For Temperature maintenance of 18-22C

 Take empty thermocol box

 Place frozen gel pack at the bottom of the box.
 Place perforated sponge (two layers)over the gel pack.
 Keep the specimen sealed in zip lock bag over the sponge .
 Then keep unperforated sponge over it & close the lid.
 Seal it & transport immediately. Indicate temperature 18-22C sample on cardboard box.

For Room temperature

 Take empty thermocol box

 Place perforated sponge at the bottom of the box.
 Keep the specimen sealed in zip lock bag over the sponge .
 Then keep second layer of perforated as well as unperforated sponge over it & close the lid.
 Seal it & transport immediately. Indicate Room temperature sample on cardboard box.

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 11. Decontamination
It is a process used for removing / killing micro organisms & removing /neutralizing chemicals.

It is carried out to prevent the spread of pathogens which may cause diseases in humans or animals.

Indications

This procedure has to be carried out before disposing the contaminated wastes like lab wastes –
specimen used for testing, used petridishes, disposable equipments,culture bottles,Human tissues or
materials containing blood , Animal tissues & Prions.

Methods of Decontamination

There are many methods of decontamination procedure and choosing any methods depends on various
factors like area ,site,substances to be decontaminated & load of contaminants.

 Physical - Dry heat -160 *C for 2-4 hours, Moist heat with pressure – eg. Autoclaving, Radiation
methods
 Chemical -Liquid Chemical (Germicides) & Gaseous chemicals & Vapours

Chemical decontamination – 1. Chlorine (Hypochlorite)

 Commonly used chemical decontaminant is Chlorine (Hypochlorite)..

 Chlorine is widely available as Bleach. It is alkaline so corrosive to metal.
 It is a Fast acting oxidant ,& broad spectrum germicide.
 Its Potency decreases on storage in open container,high temperature due to release of chlorine
gas which is toxic also.
 It should be freshly prepared to ensure optimum activity and to prevent growth of micro
organism.
 Doasge – Clean area – 0.1% ; Spillage area – 0.5 -2%
 Duration – Usual contact time is 10-20 minutes.

Other chemical decontaminants – Chloramine, Chlorine dioxide, Formalin, Glutaraldehyde 2% ,Phenolic

compounds,Alcohol.

Physical decontamination -

Autoclave – It is a pressure chamber used to sterilize solids, liquids , and instruments.

Principle -Steam act as a gas which under pressure in a closed system increases the temperature as
well as penetration thereby reducing the time required for killing microbes.

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 Autoclaving - It is a Moist heat sterilization with saturated steam under pressure of at least 15 pounds
per square inch to achieve a chamber temperature of at least 121 *C for a minimum period of 15
minutes.

 3 min holding time at 134 C

 10 min holding time at 126 C
 15 min holding time at 121 C
 25 min holding time at 115 C.

Safety Precautions

 Autoclave should be used carefully and one should know the hazards of heat, steam and
pressure .
 Trained person only operate & do the routine care of the equipment
 Do the preventive maintenance program regularly like inspection of chamber,door seals
and all gauges & controls
 Operator should wear suitable gloves when opening the autoclave though the
temperature has fallen below 80 C
 In autoclave without interlocking safety device, door should be opened after closing the
steam valve & reducing the temperature
 Care should be taken to avoid blockage of relief valve of pressure cooker autoclaves by
paper.

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 12. Safety Documents

A. Personal Risk ( Accident) Notification form

1. Physical Hazards
Minor or Major
Mechanical injury by autoclave, centrifuge, Thermal injury by water bath, fire, electrical injury,
radiation injury, injury by sharps like needle, broken glass tubes, Physical fall.

S.No Date Type & nature of physical First aid & Treatment Preventive action
injury provided taken

2. Chemical Hazards
Minor or Major
Symptoms -Local Allergy, Burns, Irritation,Systemic symptoms etc
Routes –
Inhalation of fumes , Accidental spill & splash over skin & eye, Ingestion due to poor hygiene
Type of chemicals –
Acids, Alkalis, Radiochemicals, cyanides,Xylene,Organophosphates,Methylene chloride etc

S.No Date Name,route,,Nature of First aid & Treatment provided Preventive action taken
chemical injury

3.Biological Hazards
Laboratory acquired infections – By Aerosol spread, Ingestion by poor hygiene, Accidental
inoculation by needle prick.

S.No Date Name of infections,Route First aid & Treatment Preventive action
provided taken

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 B. Biological Risk Assessment – Central Lab

1. Patients Culture report statistics – Microbiology Lab

S.No Date Nature of sample Organism identified

2. Lab Culture reports

S.No Date Lab site Organism identified

C.. Bioprophylaxis of Laboratory staff for HBV

S.No Name of the employee HBV –Vaccine HBV –Vaccine HBV –Vaccine
1st Dose 2nd 3rd Dose(6th
Dose(1month) month)

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 D. Cleaning Protocol for central lab

Area :-

Procedure – Dusting, Mopping with disinfectant

Incharge :

Daily cleaning Days with signature Weekly cleaning Weeks with Monthly
sign cleaning
1 2 3 4 5 Ist week

Date:
6 7 8 9 10 Sign:
Date:

11 12 13 14 15 IInd week
Sign:
Date:
16 17 18 19 20 Sign

IIIrd week

Date:
21 22 23 24 25
Sign

26 27 28 29 30 IVth week

Date:
Sign
31

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