CONTACT

45-1 Ramsey Road, Shirley, NY 11967, USA

Tel: 1-631-619-7922
Email: contact@cd-genomics.com
Web: https://www.cd-genomics.com

Metatranscriptomic Sequencing Service

By CD Genomics

With decades of experience in the fields of genomics sequencing, CD

Genomics is devoted to providing unprecedented amounts of microbial
metatranscriptomic data. Our strong expertise in the informative and unbiased
metatranscriptomic sequencing service is guaranteed by state-of-the-art high
throughput sequencers, flexible sequencing strategies, and professional
bioinformatics pipelines.

The Introduction of Metatranscriptomic Sequencing

Metatranscriptomics is the culture-independent profiling (including protein-

coding and non-coding DNA) of microbial community-wide gene expression,
which is capable of monitoring RNA-based regulation and expressed
biological signatures of complex bacterial communities in a given sample at a
given moment and under specific conditions. It elucidates three aspects of a
microbial community, including gene activity diversity, gene expression
abundance, and differential gene expression analysis. The gene expression
analysis can tell which genes exhibit the highest change in expression levels
in different conditions potentially to identify biomarkers and expression
signatures.

While metagenomics shows us the microbial species present in the

community and their genomic potentials, metatranscriptomics presents the
function and activity of the complete set of transcripts, as well as community
structure. Metatranscriptomics offers a more informative perspective
compared with metagenomics in revealing active biochemical functions, which
has become a focus for applications in the environmental, medical, and
energy fields as well as the field of drug discovery.

Advantages of Metatranscriptomic Sequencing

• A culture-free method to reveal the true extent of microbial diversity

• Permitting function-based activity screens
• More targeted than shotgun random sequencing
• Cost-efficient and time-effective
 CONTACT
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-619-7922
Email: contact@cd-genomics.com
Web: https://www.cd-genomics.com

• A wide range of applications, including basic research, ecological applications, clinical

applications, industrial applications, and so on

Metatranscriptomic Sequencing Workflow

Our highly experienced expert team executes quality management following

every procedure to ensure confident and unbiased results. The general
workflow for metatranscriptomic sequencing is outlined below. After RNA
isolation from qualified samples, rRNA depletion, library construction, high-
throughput sequencing, and bioinformatics analysis are in turn performed. We
can help you make decisions on the library construction method, the depth of
coverage, and the data analysis strategies based on your research aims.

Service Specification
 CONTACT
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-619-7922
Email: contact@cd-genomics.com
Web: https://www.cd-genomics.com

CD Genomics provides full metatranscriptomic sequencing service package

including sample standardization, library construction, Hiseq sequencing, raw
data alignment, down-stream bioinformatics processing and statistical
analysis. We can tailor this pipeline to your research interest. If you have
additional requirements or questions, please feel free to contact us, our
specialists are more than happy to assist you.

Reference:

1. Warnecke F, Hess M. A perspective: metatranscriptomics as a tool for the

discovery of novel biocatalysts. Journal of Biotechnology, 2009, 142(1): 91-95.