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Downloaded by [University of Glasgow] at 17:21 31 December 2014
JOURNAL OF PLANT NUTRITION, 7(1-5), 107-116 (1984)
Department of Agronomy
and
U.S. Department of Agriculture
University of Nebraska
Lincoln, NE 68583
ABSTRACT
107
INTRODUCTION
HC1 was added dropwise until the salt dissolved (pH 3.5 to 5.5),
and the volume brought to 1 liter (0.076 M ) . PDTS (5.14 g) was
dissolved in 100 ml water, 500 ml of concentrated HC1 was added,
the solution was cooled, and brought to 1 liter (0.10 tl).
water and absorbances read at 562 nm. Additional procedure and re-
agent preparation details are discussed by Pierson and Clark
(1984).
Experiment I
Experiment II
yg Fe 2 + /g dry wt.
CV (%) 26 53 45 64
Total F e 2 + 33.3 B* 41.4 A 22.8 D 26.0 C
(by addition)
'Samples for each preparation were from the same bulk leaf sample.
5 Fe/g dry wt •
Vi
Fresh* 30 .6 a 177 a 36 .0 a 60.2 a
Freeze-dried 20.4 b 154 b 12.6 b 45.3 b
Oven-dried 17.9 c 123 c 12.7 b 32.4 c
REFERENCES
Bennett, J.H., E.H. Lee, D.T. Krizek, R.A. Olsen, and J.C. Brown.
1982. Photochemical reductions of iron. II. Plant related
factors. J. Plant Nutr. 5:335-344.