Microscopy

Image Projection

Notice that the orientation of the image

you see in the lens is inverted and
reversed from the specimen you put on
the slide because of the way light travels
and is bent through the lenses.
 Magnification

As you increase magnification, you

see more detail of a smaller section
of the specimen, but you see less of
the entire specimen in one field of
view.
 Depth of Field
Notice that different color
thread come into focus at
different times as they pass
through your focal point.
 Field of View
Once you measure the diameter of the 4x objective lens’ field of view by counting the number of boxes that
cross the entire circle through the center, you can calculate the field of view diameter for the other lenses.
Assuming the 4x objective lens is “A” and the lens you’re looking for is “B”, use this formula:

Diameter B = (Diameter A * Magnification A)/Magnification B

Cheek Cell Wet Mount
In this activity, we will scrape the inside of our cheek with a toothpick and view the
cells under the microscope.
Cheek Cell Wet Mount &
Oil Immersion Procedure
 Finished? Don’t forget to:
•  Put the 4x objective lens in position
•  Lower the stage to its lowest point
•  Remove the slide
•  Clean off any immersion oil from the
lenses
•  Tie up the cord with the zip tie
•  Put the plastic cover on the microscope
•  Return the microscope to it’s appropriately
numbered shelf
•  Return all specimens/slides/equipment
back to their appropriate places.