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Forum Project Avalon General Discussion Alternative Medicine Carbon 60 (C60) Antioxidant that works at the DNA Level !

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16th July 2017 10:34 Link to Post #1

Perdido
Carbon 60 (C60) Antioxidant that works at the DNA Level !
I'll post the notes I originally took on this wonderful supplement.
Avalon Member
Join Date: 21st July 2016
Location: Pensacola, FL
Posts: 47 C60, Buckminsterfullerene Suspended in Olive Oil Benefits - Notes
Thanks: 68 C60 are carbon nanoparticles and as long as there's oil present, the C60 will greatly prefer
Thanked 209 times in 44 posts to dissolve in the oil instead. The c60 molecule has a strong tropism for lipid chains.… it
can penetrate deep into the body. C60 reacts with the fatty acids in the olive oil, thus
forming an entirely new compound.
C60 will kill Ebola virus, a Stem Cell Scientist stated !
C60 mixed in water instead of oils may be toxic !
C60 is commonly known as a buckyball and is a pure carbon molecule with 60 atoms
arranged into a spherical shape. This remarkable compound wasn’t discovered until
1985. The mechanism of action is still not exactly known. c60,buckminsterfullerenes can
conjugate with other molecules, to form complexes, but the c60 base molecule is not
going anywhere, once it is incorporated within tissue...it is either excreted, or
incorporated.
C60 is a catalyst. It is not used up in the process of its action. Many aspects of the
chemistry of fullerene are very well understood.
Aerobic- & strength gains, longevity, brain rejuvenation and tumor prevention with
lipofullerene C60 olive oil

The majority of individual human experimenters reported these beneficial effects:


More energy
Less sleep needed…. Enhanced sleep.
“Mental improvements”
Can lift much heavier weights, sometimes to the point of causing tendon injury. Commonly
reported.
Can do more reps of the same weight. This was the most commonly reported benefit.
People were adamant that there was a great difference, comparable to taking Creatine
and that not even anabolic steroids enabled such an immediate increase of both
repetitions and max. weight.
Calmer, reduction in stress
Can run longer with seemingly more effective utilization of Oxygen. Less fatigue. “Cardiac
improvements”. Better stamina and endurance.
Can run faster whilst feeling more comfortable with a pulse that would normally be too
high for steady-state.
Higher libido. This is also seen in rat trials, already with 4 micrograms/kg, a dose five times
lower than what we recommend as a daily dose.
Faster recovery of the skin after sunburn (with topical application ). 16 hours in direct
sunlight with no sunscreen on hot clear days and no sunburn anywhere ! Orally taken.
(1in50 will develop a melanoma by 2015. Over 90,000 deaths in the U.S. per year.)
Athletes (people who push themselves to their limits in sports requiring endurance,
strength, stamina) do report very significant effects, but ordinary people do not seem to
notice anything remarkable (except what we think is the Placebo effect).

1. It accumulates in mitochondria http://www.ncbi.nlm....pubmed/12054749


2. It has antiviral action
3. It is a catalytic radical scavenger - i.e. it is able to re-cycle and repeat its activity
over and over.
Study of antioxidant effect in the liver: http://www.ncbi.nlm....pubmed/16351219
4. Applied topically it appears to be able to permeate the skin barrier
http://www.ncbi.nlm....pubmed/16443688
5. Carbon 60 not only dissolves in long chain fatty acids ( i.e. in olive oil), it also reacts
with them ‘under mild conditions'. So, the olive oil-fullerene complex might have some
novel fullerene compounds that are bioactive.
http://www.tandfonli...363830701512450
6. Anti-cancer properties http://exp-oncology..../10.pdf?upload=
7. Had positive outcomes in terms of memory and ageing:
http://www.sciencedi...197458006003423
8. Had anti-inflammatory effects
9. Benefited increased hair production. Previous study … used DMSO and C60 in another
study and applying it to the skin of mice, and the result was increased hair production.
C60 have some kind of reaction since it at least stimulates hair growth...
Not the same as lifespan though. Sounds like the olive oil attached on the c60 is not what
make them live longer. Didn't everyone agree that C60 is a stable particle that attracts
free radical, because of its stable design?
The mitochondrial electron transport chain is affected beneficially by C60 – as it appears
to work by accelerating electron transfer from oxidized compounds…
* *
The c60-fatty acid adduct is the right shape, size, and hydrophobicity to become part of
a membrane, where it would be quite stable and would be expected to reside for a long
time.
* *
C60 crosses the Blood Brain Barrier (BBB) - "the presence of significant amounts in the
brain 24 hours after both oral and i.p. administrations under our experimental conditions
(Table 2) confirms that C60 can cross the blood brain barrier",
We do know fullerene passes the blood brain barrier, and has neuroprotective effects
* *
c60 is different than everything that has come before. It appears to be improving
mitochondrial function, based on the way people respond to exercise or hypoxic
conditions. This presumably means more ATP. It also appears to be decreasing the level of
ROS, based on its effect on muscle fatigue, along with its in vitro chemistry as a SOD
mimetic and ability to accept and stabilize multiple electrons ("free radical sponge").
* *
Super Antioxidant
Olive oil unlike the C60 does not modulate the antioxidant response at all, not with
Glutathione, SOD or Catalase. Also, obviously it only modulates the lifespan marginally
(18%) whereas the C60 treated rats are living 90% longer.
Chronic treatment not only reduced age-associated oxidative stress and mitochondrial
radical production, but significantly extended lifespan. Treated mice also exhibited
improved performance on the Morris water maze learning and memory task… rescues age-
related cognitive impairment in mammals.
* *
One of those biological properties it possesses is the fact that it is an extremely potent
antioxidant. Since oxidative stress and damage underlies the aging process it isn’t
surprising to imagine that C60 could help reduce aging.
This study was done to prove C60 had no toxicity and to prove that it was absorbed into
the bloodstream. The molecule can pass through the cell membrane and therefore could
be expected to have intracellular effects, critical to reduce aging.
* *
A Norwegian study that found that C60 was twice as effective as vit. E in preventing
salmon go rancid.
* *
Average life of a rat is 2-3 years.. the C60 feed rats lived 5 years in good health with no
signs of cancer. The oldest lived rat in the C60 group actually lived to 66 months which
appears to be a record.
The study remarks how it is normal for Wistar rats to die of tumors or pneumonia, and how
the C60-treated rats neither developed cancer nor lung problems – they simply died of
general organ failure associated with old age – they reached their genetical maximum
lifespan, their weight was that of a normal healthy middle-aged rat until close to the end.

The rats did not just live very, very much longer (years after they should have died of old
age!) but their livers were fully protected against lethal doses of highly toxic chemicals.
..See photos of how the C60-protected livers look healthy, whereas the livers of rats that
did not get C60 got destroyed.

* *
It is suggested that C60/EVOO is stimulating stem cells, and that is likely happening by
the stimulation of mitochondria. If so, the same process would cause cancer cells to
revert into normal cells since cancer cells in general shut down their mitochondria and
thereby get some of the advantages of stem cells--eg, immortality.
* *
A patent application by researcher Dr. Christopher Kepley suggests one mechanism by
which c60 fullerenes might extend life: Method for Inhibiting the Build-up of Arterial Plaque
by Administering Fullerenes

http://appft.uspto.g...=DN/20110190251
And what good is long life if you must live it bald?

Fullerene nanomaterials potentiate hair growth. "As seen in Figure 2, A and B, mice
injected with fullerene derivatives had accelerated hair growth as compared with those
mice receiving vehicle only."

http://144.206.159.1...25/12825719.pdf

* *
The major theories how c60, lipofullerenes’ anti-aging effects are:
(1) C60 remains in the lipid bilayers of the cells and mitochondria, doing its ROS-
neutralizing work for weeks or months.
Lipofullerenes could act as super-antioxidants on the cellular level, where they remain for
a very long time, since they are not “used up” when they act as antioxidants, and they
are constantly recycled in the cell.
(2) The C60 physically rejuvenates organisms on the cellular level, for example by DNA
demethylation or by otherwise reversing cellular damage on the molecular level.
Interestingly, the size of the Buckyball C60 molecule is such that it fits exactly inside the
“minor groove” DNA winding and it indeed binds to it, protecting it against Methyl groups
that degrade and neutralize the DNA:

Here are some postulations made in the Longecity forum on how C60 may extend healthy
life:
(3) “Attenuation of age-associated increases in oxidative stress.”
(4) “I’m wondering if the fullerene might not be the active longevity ingredient. Maybe it’s
just the transport. And dissolving it in olive oil also allows some olive oil molecules to
penetrate the fullerene sphere and get transported to places by the fullerene that orally
ingested olive oil wouldn’t make it to. Like deep into many cells throughout the body even
those across the bbb. It that’s right, all the longevity effects observed are just an
amplification of the effects of the olive oil.”
(5) “My guess is that it binds with toxins.”
(6) “It is a catalytic radical scavenger – i.e. it is able to re-cycle and repeat its activity
over and over. It accumulates in mitochondria. It has antiviral action. C60 has anti-cancer
properties. Possibly, as with methylene blue, there might be a hormetic affect – with low
dosages having a positive effect, and higher doses a deleterious one.”
(7) “Maybe enhancing neurite growth sufficiently enhances feedback signaling in the body
and that is what results in an enhanced immune response.”
(8) “Since the mechanism of action is still unknown we have no idea if the buckyballs are
destroyed in the process of performing whatever function they are performing. Maybe
they somehow bust up AGEs and are destroyed in the process. Maybe they settle in and
become a conduit for some biophotonic reaction.“
(9) “We do know fullerene passes the blood brain barrier, and has neuroprotective
effects.”
(10) “C60 is a catalyst. It is not used up in the process of its action.”
(11) “C60 is able to associate with mitochondria and induce a cytoprotective antioxidant
effect.”
(12) “C60 might be resetting the mitochondria to a younger age.”
(13) “We humans have been cooking our food for a long time and the soot from these fires
maybe contained fullerenes. Perhaps fullerenes are an important part of the paleolithic
diet.”
(14) Here’s a sketchy hypotheses of how C60 might work:
Fullerenes wrapped in oil are transported to mitochondria for burning.
Once there, the oil is stripped and the naked fullerenes are attracted to other hydrophobic
molecules, such as the methyl groups on the mitochondrial DNA, and reacts with them,
stripping them from the DNA.
Demethylation of the DNA thus reverses the epigenetic DNA changes that result in
dysfunction of the mitochondria.
(15) There’s been some mention of mitochondrial biogenesis in this thread… Is this
something that fullerenes are known to do? Mitochodrial biogenesis means the creation of
new mitochondria.
(16) Fullerenes are known to like methyl groups, and that they could steal them from DNA
would certainly provide a tidy explanation of how this works.
(17) Fullerenes might exist in a more longer-lived reservoir, like in the membrane of a long-
lived somatic cell.
(18) My hypothesis is that fullerenes wipes out the methylation, the DNA automatically
recovers it, and the effective age is set to zero. The DNA will begin to age again, and a
later treatment can reset it back to zero. Keep resetting every few years and the ultimate
limit then passes to the telomeres and epigenetics of the nuclear DNA.
(19) It’s very clear that C60 has mitochondrial activities; for example, animals treated
with C60 are more resistant to radiation effects, and are far more resistant to the
oxidative damage from carbon tetrachloride ingestion. That isn’t just a reset to a youthful
state, because youthful animals would be injured by both radiation and CCl4.
(20) Olive Oil is a 5-LO inhibitor. 5-LO inhibition reduces ROS, independent of P53. I
cannot escape the conclusion that the choice of the Olive Oil solvent, per se, cannot be
dismissed as being unimportant to the Buckyball study conclusion.
(21) I have a hypothesis: Because of its hydrophobic propensity, C60 incorporates
itself into cell membranes thereby presenting the adducted olive oil which disrupts the
signaling pathways of arachidonic acid in the phospholipids therein while also sopping up
supersoxide everywhere the C60 finds it, nice “one-two”. How/why does the C60 pass
through the cytosol into the mitochondrial membranes? Wouldn’t it want to stay in the cell
membrane? Is it shunted through the cell membrane by a channel protein or an inner
membrane channel protein? Is there a charge graident involved? Is it just possible that
because it behaves like a wave also…it makes for the mitochondria and leaves the olive oil
in the cell membrane?
(22) Harman, in this 2001 paper posits that fullerene may work in the mitochondria as a
blocking agent. Many antioxidants,at therapeutic doses, actually decrease the efficiency
of the mitochodria, which is why possibly we do not see increases in the maximum
lifespan. If fullerene is indeed a blocking agent, then it acts by stopping oxygen from
generating reactive oxygen species and superoxide formation, while having no negative
impact on mitochondrial functionality. Other studies posit it is a superoxide dismutase
mimetic.
(23) Drugs, diet, or exercise are all capable of changing gene expression; for example,
resveratrol alters the expression level of on the order of 1000 genes. It’s very likely that
fullerenes change the expression level of numerous genes as well. For the vast majority
of these genes, the changes in expression are transient, and when the drug is withdrawn,
expression returns to normal. In some cases, however, there are apparently long-lasting
changes put in place by CpG (de)methylation, histone (de)acetylation, etc. These are
epigenetic modifications.
(24) ..the Fullerene Solvent served as the means for “guiding” the Fullerenes to the “cell
type” that the solvent has a natural affinity for, to the appropriate “receptor” of that cell
type..
(25) C60 is thought to be a “SOD Mimetic”, SOD is an enzyme that clears out the
destructive superoxide ROS in your cells.
(26) I suspect that the C60 that finds its way into non-mitochondrial membranes isn’t
hurting anything, and may be helping some, but the fraction that gets into mitochondrial
membranes is what’s doing the magic, because it’s close to the source of damaging
superoxide ions.
(27) I think that the conjugation of fullerene with molecules present in olive oil is crucial –
and it makes no sense whatsoever to tinker with the basic formula when the knowledge of
what is going on is basically non-existent. If you are going down this route, stick to olive
oil.
(28) I wouldn’t say that we don’t have a clue how this works. I’d say there are some
hints. We know it’s redox active, and that fullerene carboxylic acid compounds can act as
SOD mimetics, turning superoxide radical anion into oxygen and H2O2. We know that
superoxide is generated in mitochondria, and it’s at least reasonable to suspect that a
fullerene fatty acid adduct could become part of the mitochondrial membrane.
(29) I don’t think it is a stretch to assume that the positive effects of C60, if any,
are tied to its relative inertness.
(30) That it is the Olive Oil combination with C60s that extended life spans in the
surprising way it did suggests that our innate Immune System driven CAIP is implicated in
some way in the study result.
(31) A resetting of the Mitochondrial DNA in the gut related to our Innate Anti-
Inflammatory mechanisms resets our ability to manage the auto-immune diseases (like
cancer) which, increasingly, knock us off as we age.

¤=[Post Update]=¤

C60 Review by Turnbuckle


http://www.longecity.org/forum/user/19769-turnbuckle/
Updated 8/12/14

This page is about subjects as diverse as dental care and Methylene blue & Alzheimer's,
but is mostly about C60 for age regression. C60 in EVOO is the best supplement I've taken
in the past forty years. In the sections below I've documented the benefits I've seen as
well as potential dangers, and speculations on possible modes of action. Feel free to write
me about any of it.

Sections—

1. C60 usage
2. C60 effects
3. C60 Age regression
4. C60 potential dangers
5. C60 epigenetic hypothesis
6. C60 and glutathione
7. C60 stem cell hypothesis
8. C60 and cancer
9. C60 prep (and where to buy)
10. Creating new stem cells in vivo
11. The Turnbuckle Protocol
12. Mitochondrial supplements
13. Other supplements, a very partial list
14. Dental
15. Methylene blue & niacin & Alzheimer's
15. Methylene blue & niacin & Alzheimer's
16. Not recommended (eg, statins)
17. Personal data
----------------------------------------------------------

----------------------------------------------------------
1. C60 usage
----------------------------------------------------------
First use of C60 on 4/27/12

Present experimental dose as of 5/1/13: 4 mg C60-EVOO, once a week

----------------------------------------------------------
2. C60 effects
----------------------------------------------------------
Greater stamina/oxygen utilization. This was within hours. As I'd been damaged by statin
use, others might not see such an immediate effect. Other effects took longer, ranging
from days to months.

More youthful skin. Skin pinch test went from 1-2 seconds to 0 seconds. (I had very
youthful skin to begin with.)

Two small scars from 8 years ago have disappeared. Two similar scars of much older
vintage did not.

Hair regrowth that filled in a patch in the back that had been thinning for thirty years and
was bare in a spot about as big as my palm. (A Norwood 4 in the back and a 1.5 in the
front). With C60, this occipital spot filled in to what I would call "thinning," which is to
say, scalp is still visible and needs about twice as much hair to make it invisible. Further
improvement--if any--has been very slow. See the Luna patent application--
http://appft.uspto.gov/netacgi/nph-P...DN/20110003773
Update (12/3/13): I have now seen further improvement by applying a small amount
dissolved in 91% rubbing alcohol, applying it with a dropper to the scalp for a few days.
So topical treatment may be better than oral for hair regrowth.

Stronger nails, which had become prone to splitting. I first noticed this at about 4 months.
(Both follicle roots and the nail matrix contain stem cells, which appear to be stimulated
by C60.)

Much better flexibility. This has been slow to develop but is now quite apparent.

Better exercise tolerance. Upped weights on machines by 1/4 to 1/2 and increased runs
up to 4 miles (from essentially nothing for several years).

Much better alcohol tolerance while taking C60.

Subjective improvements in smell, high frequency hearing, peripheral vision. Note: There is
a patent on the improvement of hearing with a C60 derivative --
http://patentscope.wipo.int/search/e...n=US2005028171

UNCHANGED: Taste, height, weight, appetite, blood pressure, cholesterol, blood sugar,
hair color, sun response.

DOGS: I've given C60 a few times to my middle-aged, unrelated dogs (.18 mg/kg). One
recovered his ability to jump into the car within 2 days and catch tennis balls in the air,
and the improved jumping has lasted months with no further treatment.

----------------------------------------------------------
3. C60 Age regression
----------------------------------------------------------
30 year regression -- alcohol tolerance
20-30 year regression -- bald spot in back
20 year regression -- sugar tolerance
10 year regression -- thinning on top
10 year regression -- skin
10 year regression -- oxygen utilization while running
0 regression -- broken capillaries
0 regression -- hair color

In general, effects fell into three categories: immediate, delayed by months, and
incremental. The immediate effects were to oxygen utilization and gym performance, the
delayed effects to the nails and hair, and the incremental I'd characterize as a Benjamin
Button type biological time reversal. In my case C60 not only stopped aging, but seemed
to slowly reverse it. Of course, I'm only a year and a half into it at this point, so nothing
definite can be said about long term effects.
definite can be said about long term effects.

----------------------------------------------------------
4. C60 potential dangers
----------------------------------------------------------
I've taken it more than 5 days continuously twice and both times found a fading of
effects. Others have seen this as well.

Taking C60 + 1 gram niacin made me feel weird for a week. A statin-like feel.

A C60 extract containing 28% C70 & 2% higher fullerenes at a very low dose level (0.5
mg/day) resulted in steady pains in both calves after a few days, as well as several hours
of pulsing pain in the back of my neck. This went away within a day or two of stopping.
Thus, for me, C70 is out. (One study found C70 in the endoplasmic reticulum (ER) rather
than the mitochondria. In the ER it might interfere with protein synthesis.)

Ligaments don't improve as fast as endurance and are prone to injury. So just because
you can now run twice as far or lift twice as much, don't. Best work into it slowly.

C60 may be encouraging the differentiation of stem cells, and thus too frequent use may
deplete them. (I've been told that depletion is impossible. Maybe so.)

----------------------------------------------------------
5. C60 epigenetic hypothesis
----------------------------------------------------------
While C60/EVOO is certainly an anti-oxidant, there is also a spectrum of effects that
appear to be persistent if not permanent, thus it is possible that C60 is modifying the
epigenetics of the mitochondrial DNA (mtDNA).

The epigenetic content of mtDNA is mostly the result of methyl groups (PMID: 21321201)
that attach to DNA and act as off switches. These are copied after DNA replication to the
daughter strand by methyltransferase, which must contact both strands to copy the
switch positions. It is believed from a theoretical study (PMID: 16183879) that C60 is
attracted to the DNA groove between strands. There it may act as a physical barrier--a
methyltransferase inhibitor like procaine*, which is also attracted to the DNA groove and
has been shown to extend the lifespan of Wistar rats.

If the methylation of mtDNA is randomly reduced (and thus has fewer genes turned off) in
some fraction of mitochondria this will prove to be advantageous and the population of a
cell’s mitochondria (of which there are typically hundreds or thousands) will in time
become dominated by the better functioning mitochondria as they are constantly dividing
and the defective ones removed by mitophagy (an article on mitophagy--PMID:
22743996). Thus it would be important to take regular breaks from C60 treatment to allow
the population of better functioning mitochondria to increase without further modification-
-a dosing schedule the Baati rat study (PMID: 22498298) may have stumbled upon by
accident.

This process of replication and mitophagy is selection of the fittest at the intracellular
level. Taking C60 continuously may result in degrading the better functioning mitochondria
as it doesn't allow time for the selection process to work.

There is also a process of mitochondrial sex involving fusion and fission whereby the
double-stranded loops of mtDNA (of which there may be ten or so per mitochondrion) get
randomly exchanged so that after fission a mitochondrion might have a perponderance of
defective strands and thus functions poorly and is recycled.

*Procaine is sold for longevity as "Gerovital". In 1965 researcher Anna Aslan reported that
it increased the lifespan of Wistar rats (males only) by 20%. The dosing schedule was
intermittent with injections of procaine 3 times a week for 4 weeks, followed by 4 weeks
off. According to PMID 12941824: "Procaine can also demethylate densely
hypermethylated CpG islands, such as those located in the promoter region of the RAR
beta 2 gene, restoring gene expression of epigenetically silenced genes. This property may
be explained by our finding that procaine binds to CpG-enriched DNA."

----------------------------------------------------------
6. C60 and glutathione
----------------------------------------------------------
The up-regulation of mitochondria by C60 will create more ROS, and in some people the
endogenous antioxidants such as glutathione may become depleted. Therefore,
supplements that increase these antioxidants may help, such as NAC. Taking 1800 of NAC
with 2 mg C60 did in fact help in the gym, but ultimately did not keep the effects from
fading with continuous use. It also has a reflux problem. I've recently found that reduced
L-glutathione works very well and I'm now taking a gram a day, along with 3 grams of
vitamin C, 2 grams of niacin, and Pepcid AC. I don't have very many age spots, but the
ones I have seem to be fading with this treatment.
ones I have seem to be fading with this treatment.

----------------------------------------------------------
7. C60 stem cell hypothesis
----------------------------------------------------------
A user here (mpe) has reported that the use of C60 “was like having the stem cells
[injection in his knee] all over again.”

This is perhaps to be expected since C60 has been observed to collect in mitochondria
and many here have reported that C60 enhances endurance (and most probably
mitchondrial effectiveness). In fact, healthy stem cells have a much reduced mitochondrial
mass and oxygen consumption and depend on anaerobic respiration, much like cancer
cells. Thus C60 treatment may be encouraging stem cells to differentiate by increasing
their mitochondrial mass. It might do this by turning on mitochondrial genes that are
turned off in stem cells. In fact, it is known that stem cell mitochondria changes first and
signals the nDNA. (PMID: 21425411)

Would stem cells be used up by this process? Perhaps not as they have mechanisms to
prevent that, such as asymmetric replication whereby the original stem cell divides into a
stem cell and a daughter cell that is differentiated. Still, it's something to watch out for,
especially in young users of C60. (How do you watch out for it? I have no idea, but see
section 11, below.)

This stem cell hypothesis is not inconsistent with the epigenetic speculations above, as it
might operate by the same mechanism--turning on genes by preventing their methylation
by methytransferase. Strengthening of nails, hair regrowth, and the disappearance of
small scars that I have observed from my own use could be the result of stem cell action.

Another possible interaction re stem cells is with uncoupling proteins (UCP). These
proteins are valves that switch mitochondria into a heat producing mode, and are highly
expressed in stem cells, thereby keeping them as stem cells. If C60 could inhibit such
proteins, it could stimulate stem cells proliferation and differentiation. It might also do this
in cancer cells (which switch their mitochondria in a similar fashion to stem cells), thereby
forcing them to become somatic cells again--differentiation therapy. There's no particular
reason to expect C60 to function as a decoupling protein inhibitor except for the
congruent geometry of the protein and the C60 molecule, and that both can be found in
the mitochondrial wall and the effects noted by some users are consistent with the
stimulation of stem cells.
http://newsroom.ucla.edu/portal/ucla...er-219140.aspx

There are at least three types of UCP, with UCP1 having a half-life of days as compared
to UCP2 and UCP3, with a half-life of an hour or so. (PMID: PMC3661275) However, UCP1
is mostly expressed in brown adipose tissue, not muscle.

----------------------------------------------------------
8. C60 and cancer
----------------------------------------------------------
In some ways cancer cells are like stem cells, and sometimes are stem cells. They are
immortal and achieve immortality by shutting down (or more precisely, decoupling) their
mitochondria and resorting to glucose fermentation for energy (the Warburg Effect). They
also become immune to apoptosis (cellular suicide) because active mitochondria are key to
setting this housekeeping function into motion. So if C60 restores mitochondrial ATP
production, it would also restore apoptosis and that would destroy the cancer cells. Thus
C60 might provide a two pronged approach to neutralizing cancer. (See my remarks on
"differentiation therapy" in the section above.)

----------------------------------------------------------
9. C60 prep (and where to buy)
----------------------------------------------------------
If you just want to try C60 or anticipate using only small doses as I do, it's better to buy
the solution ready made. There are five sources I know of, four in the US--

http://www.carbon60oliveoil.com/
https://sesres.com/C60-olive-oil.asp
http://www.buckylabs.com/
http://www.maxamlabs.com/shopexd.asp...1#.UnY2Wvl97za

and another in Switzerland--

http://shop.owndoc.com/p-203/c60-oli...ls-in-oil.html

I've bought one bottle from the first listed company and the quality appeared to be
excellent. The second is from the largest manufacturer of fullerenes. The fourth has a
very high price.
If you plan to use a lot you might consider making your own. But keep in mind that C60 in
oil oxidizes with time, and it's not clear how that affects its usefulness (but I think it
cannot be good).

First of all, the oil. Don't go by color. Pick an extra virgin oil packaged correctly, in a dark
colored bottle. These are typically green or amber glass. Buy organic if you can find it,
and deaerated. California Olive Ranch didn't generate bubbles under a vacuum while
filtering, so it was probably deaerated. Also, California Olive Ranch wasn't found to be
mislabeled like many oils. See the following UC-Davis report where most oils labeled extra
virgin actually aren't. Scroll down to Table 2 (on page 6).

http://olivecenter.ucdavis.edu/what-...%20reduced.pdf

I grind the (SES) powder in a stainless mortar then magnetically stir it in the EVOO and
filer at .22 micron (using an Autofil unit). I have a centrifuge but haven't seen the need
for it. This process takes about 3 days or less. Once prepared, I keep a small amount in
the fridge and the bulk of it in the freezer. The oil is initially greenish-yellow and with C60
it is red. In the freezer it becomes a hazy strawberry and eventually lilac. On defrosting, it
returns to its red color.

Recommendations—

What concentration to make: 0.8 mg/ml (800 mg in 1 liter).

How much to make: 1 liter for personal use. This can be made in the original bottle by
dropping in the magnetic stirrer and recapping, thus minimizing oxygen exposure.

How much to take: 4 mg once a week (5 ml). The best protocol is currently unknown, but
more seems to be less.

Does it go bad? Probably.

What’s the shelf life? Probably six months, but better to use it up in three.

What’s the best way to store it? The rate of chemical reactions typically drop by half for
every 10° C. So in a -20°C freezer, the oil should last 16 times as long—3 months on the
kitchen shelf being equal to about 4 years in the freezer. I recommend buying amber
colored glass bottles from Amazon and dividing the prepared oil for freezer storage, only
keeping one bottle on the shelf for use.

Bottles: http://www.amazon.com/Amber-Glass-Bo...d_sim_indust_5

Other sizes are available.

----------------------------------------------------------
10. Creating new stem cells in vivo
----------------------------------------------------------
If C60 uses up stem cells as postulated in section 7 above, can more be made? Possibly.
Recently it was discovered that mouse somatic cells could be reverted back into
pluripotent cells by application of chemical stressors, such as acid. (See Wikipedia for
"Stimulus-triggered acquisition of pluripotency cell".) Charles Vacanti, the lead researcher,
said, “We looked for a more natural way to turn mature cells back to stem cells, and it’s
actually very efficient, easy, and inexpensive to do. You get millions of cells very quickly,
and you should be able to use it in all fields of medicine, boosting the function of tissues,
possibly saving lives.”

His method treated blood with HCl, but could the same be done with direct application of
an acid to the skin? This is what I'm trying now, using apple cider vinegar. This isn't new
at all, and has been reported to be good for the skin, nails, and hair, with various
rationales offered for its efficacy. But what if it was generating new stem cells? Which
now seems more likely than not, at least for some of the claimed effects.

----------------------------------------------------------
11. The Turnbuckle Protocol
----------------------------------------------------------
I am presently combining 4mg C60 with 2 tablespoons of 20ppm colloidal gold (trying 2
brands--Medigold and Mesogold). This has an enhanced effect in the gym and the gold
definitely has nootropic effects.

Once a week, sometimes twice.

For skin I am wet brushing with a stiff nylon bristles. This seems better than dry brushing
and better than dermarolling for reducing crosslinking.
Once a week I apply cider vinegar all over, let it dry, and wash it off. This has definite
positive effects, and may create new stem cells, thus complementing the effects of C60.

----------------------------------------------------------
12. Mitochondrial supplements
----------------------------------------------------------
PQQ, 10 mg, every other day (Has been shown to enhance mitochondrial biogenesis)

CoQ10 .5 to 1 gm (Mito antioxidant and essential enzyme)

ALCAR/ALA, 1gm/100mg (Various reasons, synergistic with CoQ10)

Lithium Orotate, 120 mg twice a week (Lithium supports mitophagy and has been shown
to increase life span of animals and humans)

Melatonin is a brain hormone and mitochondrial antioxidant produced by the pineal gland.
This gland calcifies with time and by the age of 50-60 a supplement may be needed.
(Note that fluoride in water and toothpaste may accelerate the calcification.) I take 5 mg
time release.

Astragalus extract, 500 mg (Has been shown to act as a mitochondrial antioxidant and
might lengthen telomeres of nDNA). GreenPower has an interesting post about the testing
of telomere lengths for several astragalus-related supplements--
http://www.longecity.org/forum/topic...30#entry485926

----------------------------------------------------------
13. Other supplements, a very partial list
----------------------------------------------------------
Spermidine -- 50 mg once a week in grapefruit juice. Enhances autophagy, cell growth,
energy level, strength, skin tone, hair color and growth, and potentially, longevity. Based
on my limited experience with it to date, I'd say it's best not taken with C60. I've seen
many of the effects above, but no change in hair color.

http://www.longecity.org/forum/topic...rmidine-trial/
Note that spermidine could possibly cause herpes outbreaks in those infected, since the
virus contains that polyamine.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC389533/

Cinnamon extract for improved glycaemic control.

Magnesium ascorbate, 1 gm daily (magnesium supports mito function and brain function.
I've tried magnesium threonate and it was quite invigorating the first time I used it, but
not with subsequent doses, so I'm not sure there is anything special about it over the
long term)

Ginger root extract + MSM -- for inflammation of a swollen bursa. Most effective.

Silymarin (milk thistle extract) -- for liver health and might lengthen telomeres of nDNA.

Hormonal support: DHEA, pregnenolone, and, for testosterone, tribulus and fenugreek

----------------------------------------------------------
14. Dental
----------------------------------------------------------
Strontium citrate twice a week and strontium chloride mouthwash (homemade--20%
anhydrous SrCl2 in water) once or twice a week. Result: no cavities in a decade. I take
EDTA before and after every dental appointment to chelate mercury from amalgam fillings.
I have very few such fillings now and feel better as a result.

Am now brushing with curcumin (or turmeric) twice a week in the shower for the whitening
effect. Also very good for gums. Adding an equal part ascorbic acid to it makes it more
effective, especially at whitening, though I use the acid very rarely.

----------------------------------------------------------
15. Methylene blue & niacin & Alzheimer's
----------------------------------------------------------
A few years ago (after the stories about Rember came out) I tried a dose of MB. I didn't
have Alzheimer's but it seemed like a potentially good prophylactic as everyone my age
probably has some of this defective tau protein. In fact, I found that I was thinking more
clearly with a dose of 100mg, but when it wore off I was suddenly having difficulty
following the plot of TV shows. Uh-oh! So I looked for something that would correct the
defective (hyperphosphorylated) tau that causes tangles and tried MB again a couple of
days later, this time adding 500 mg niacin every few hours. The problem went away and
when the MB wore off I was no worse off for the experience. So I realized that seemingly
modest doses of MB can be dangerous, that even if you don't have symptoms of AD you
might create them by forming tangles that didn't previously exist. On the plus side, it
seemed possible that MB+niacin is a cure for AD, if the MB dissolves the
hyperphosphorylated tau tangles and allows niacin (or niacin analogue like niacinamide) to
get access.

PMID: 18987186 -- "These preclinical findings suggest that oral nicotinamide [niacinamide]
may represent a safe treatment for AD and other tauopathies..."

Other supplements that have been shown to mitigate tau pathology: sodium selenate and
propranolol. I take both.

Presently I do not take MB. Instead I take 2 grams of niacin in one morning dose (pepcid
ac is also helpful since niacin enhances HCl production). I take this as a prophylactic
against Alzheimer's and to raise HDL. My wife now takes niacinamide as a prophylactic
against Alzheimer's, and it has improved her memory.

----------------------------------------------------------
16. Not recommended
----------------------------------------------------------
Resveratrol--long term use of 200mg daily caused severe joint pain. I now seem to be
sensitized to it as even a small dose causes joint pain after a few days. I can't say if it's
the resveratrol itself or some impurity, but I am avoiding it in any case.

100 mg Methylene blue--created symptoms of AD until I reversed it by taking MB with


niacin. See the Methylene blue section, above.

Activated charcoal and intestinal bug--this made the bug much worse as the charcoal felt
like broken glass.

Naproxen (Aleve)--causes tinnitus.

Statins--mitochondrial poison that should have a skull and crossbones on the packaging.
I've tried three. All slightly different in feel. All bad. And perhaps counterproductive as
well. See the second figure in this paper--

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303886/

--where the death from all causes chart shows that for women, the higher the total C,
the better, and for men, a sweet spot centered around 210 total C--where I am with no
drugs and a diet that eliminates red meat.

I like this quote by Stephanie Seneff--

"Every individual gets at most only one chance to grow old. When you experience your
body falling apart, it is easy to imagine that this is just due to the fact that you are
advancing in age. I think the best way to characterize statin therapy is that it makes you
grow older faster. Mobility is a great miracle that cholesterol has enabled in all animals. By
suppressing cholesterol synthesis, statin drugs can destroy that mobility. No study has
shown that statins improve all-cause mortality statistics. But there can be no doubt that
statins will make your remaining days on earth a lot less pleasant than they would
otherwise be."

Source: http://people.csail.mit.edu/seneff/w...ally_work.html

----------------------------------------------------------
17. Personal data
----------------------------------------------------------
Age-- >60
Weight-- 82 kg
Employment--previously, senior scientist for a research company; presently, novelist

Photo: Orson Wells

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16th July 2017 10:35 Link to Post #2

Perdido
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
C60 Surprises - Anecdotes of Unique Health Benefits
Avalon Member
Join Date: 21st July 2016 Feedback.. from users about C60 & health.
Location: Pensacola, FL
Posts: 47 Quotes..
Thanks: 68
Thanked 209 times in 44 posts http://www.longecity.org/forum/

C60 is powerful stuff and we have only just begun to find out what it can do.

As someone noted from a study, C60 is out of the body in about 96 hours and that's what
I noticed myself.

One class of things I'd avoid taking at the same time as c60 in evoo are those designed to
impede digestion of fats and olis.60; Dietary fiber like oat bran,60; psyllium, chitosan, or
glucomannan.60; I understand that Vitamin C tends to make dietary fiber even more
effective at removing lipids from the digestive tract.60; It may go without saying, but if
you take any cholesterol medications, it probably wouldn't be a good idea to wash them
down with olive oil either.
* *

...the antioxidant nature of C60 is that it doesn't get 'used up', the way a chemical
antioxidant like tocopherol or ascorbate might. C60 has a fairly low reduction potential,
and electrons can flow on (and then off) without a lot of difficulty. In other words, C60
gets regenerated very easily, while more typical antioxidants need a more potent reducing
agent to get recycled.
* *

C60 doesn't work by removing waste; It detoxifies the free radicals before they can
create the "waste" (oxidized molecules, which are not themselves free radicals); The first
like is a report about the Baati et al. paper that described the results of the serendipitous
discovery of c60-olive oil.60; You can get a copy of the paper here. (at the end of the
article)

http://extremelongevity.net/wp-conte...-Fullerene.pdf

Without reading every paper, the impression I get is it doesn't work for neurons and
retinal cells, but may work for other cells, such as muscle cells.

* *
Anyway, besides spinal pain I feel better than before experimenting with C60 and few
other substances - including mental abilities and fatigue resistance.

* *
It's possible that your spondylitis will improve over time when using c60. I had a long-
standing neck problem due to an old injury- two discs were very thin- but after being on
c60 for quite a while, I have no more pain. I've been using it for about two years; I'm not
sure exactly when it improved.
The improvement I saw in my eczema was pretty rapid. The spondylitis improvement that
I've experienced happened over months of use.
* *

What most impressed me with C60 was not the life extension per say so much as the
transport mechanism I suspect it is. It was very mind blowing to see the rats
given carbon tetrachloride and not being liver damaged. This indicated to me it could be a
transport mechanism of a high order and maybe what gave the olive oil such a kick for
extra longevity. If it is then it would help transport nutients into the cell and wastes out. I
also suspect it will stay in the body as a transporter since it might not chemically react to
anything. I have no scientific or chemistry backround other than just trying things.

* *

Well the only effect I feel sure about is a dramatic increase in caffeine tolerance. I've had
7 cups and I'm only starting to feel the coffeehigh. Weird
(Reports of Alcohol tolerance is also reported.)
When I ran out of it, after about four days (~96 hours) alcohol started working better.
When I resumed taking C60oo alcohol stopped working as well.
When I resumed taking C60oo alcohol stopped working as well.

* *

I'm now dosing twice a day 1 mg per dose after noticing an immediate increase in mental
clarity/energy holding the C60/OO sublingually and swishing it around before swallowing
and I like the effect.

* *
Unless you're cycling with an off period of months, I don't think that you are ever "off", as
far as the important mitochondrial effects are concerned. There are several different
activities that have been noted with c60oo, and they have different dose requirements or
half lives. The improved oxygen utilization effect seems to require the lowest dose, and
last the longest. The immunomodulatory effect that improves cases of asthma, allergies,
and eczema appears to be due to a minor oxygenated component in the c60oo, and
requires a larger dose to manifest, so it drops off more quickly. From Mikey's description,
it sounds like the suppression of the high from alcohol requires a larger dose and/or wears
off faster. At the moment, I'm taking ~25mg/month, at two week intervals. In the past
I've used as much as a four week interval between doses.

* *

I'm not sure if "dependency" is the right word, but I don't want to go back to the way I
used to be. I very much like the way c60 makes me feel, and there doesn't seem to be
much downside to taking reasonable doses. For me, c60 is a medicine that fixes problems I
couldn't fix any other way. It's also a performance enhancing drug. It makes me healthier
and costs less than I spend on coffee. (A lot less, and I don't buy expensive coffee!) So
why would I want to stop using it? Maybe that sounds like the alcoholic who says "I can
quit any time I want. I just don't want to." Well, if this is dependency, that's cool. I hope
I find some more things this good to be dependent on.
* *

So far, what i've noticed most since starting c60-oo is a reduction in allergies.

Usually I have pretty severe pollen allergies, sneezing 10-20 times on a rough day.
Irritated, scratchy inner throat is also common for me, and it gets especially bad when
visiting relatives out of state, in MS. However, I just returned from such a trip with pretty
much no allergy symptoms at all, which definitely seems to be an improvement for me.

Albeit, I haven't been there in a few years and my reduction of allergies could be a result
of a couple years of supplementation/epigenetic changes, I think it is more likely the result
of my C60supplementation.

.........You're not the only one. Other fullerene analogs have been shown to cause a Th2
to Th1 shift, which moves your immune system away from allergy and atopy. To me, this
is one of the more important effects of c60oo. I don't have rhinitis, but I have eczema
and an atypical (non-reactive) asthma. C60oo eliminates all of my eczema symptoms and
significantly improves my small airway function according to spirometry. All this is
consistent with less Th2 bias.

* *
I have noticed that my stiff knee has improved since upping my dose of C60. Used to be
that it took a while to loosen up in the morning and I would limp around for a bit. Suddenly
I realized that I don't do that anymore. Also it seems that my blood pressure is always in
a good range now. In fact it is rarely over 115/65. I used to have days when it was higher
than I would have liked. Blood pressure depends on many things so it's hard to know for
sure - but I can't think of anything else I'm doing differently lately.

* *
Not reported in for a long time - about 2 years ago, after finding that my endurance in the
gym increased dramatically, I changed my exercise regime to very high repetitions - with
high weights. I experienced no pain, and was reallyhaving a wonderful time in the gym
throwing weights around. This high rep-high mass regime lead to a severe case of sudden
onset tendonitis, which took almost 18 months to calm down. I am now back at the gym -
I have been back for 2 months now - and the affected tendon seems to be fine.
60;
This discussion on oxygenation of the blood is interesting - it may be one of the
mechanisms that explains some of the effects we see with C60OO dosing, although I am
of the opinion that the proposed mitochondrial effects are the primary candidate.
60;
60;
Interestingly, over the 18 months, while doing no working out, I lost very little muscle
mass.
60;
I restarted gym and have rapidly returned to previous strength levels. I am gaining mass. I
am lifting higher weights, and doing lower reps in each set - no more than 10 reps per set.
I don't get pain while working out, I just reach a point where I simply can't lift the weight
for love or money.......and I have almost no post-gym pain - despite over 18 months of
going no-where near a gym. This effect I put down to C60OO.60;
60;
I am still taking approx 1mL of fullerene OO daily - I am still using my initial homemade
batch, which has not run out yet. I don't measure the dose precisely, and I vary it slightly
from day to day.
60;
I find that on days I miss my dose, my endurance when cycling flags somewhat - I
particularly notice this on the uphill cycle climb on the way to work. I am now 48 years
old, 49 in August. Prior to starting fullerene OO, I used to really struggle with my cycling,
despite being fit, it was getting harder and harder each passing year. I am now almost
never get that 'muscle burn' feeling when cycling.

* *
I'm 77 and have been taking taking C60oo for six weeks. 60;I started at a rate of 15mg
every two weeks but am switching to 15mg every 10-12 days because the effect appears
to wear off at about that time.60;When the effect is wearing off I experience energy loss,
back pain, and my life becomes less pleasant.60;All of which vanish within an hour of
taking another dose.
60;
Overall I have nothing novel to report. 60;My experience with exercise and conditioning,
energy level, sense of wellbeing, etc., has been similar to that most posters have
reported.
60;
My sexual activity has continued to be good for my age but with no change.

* *
True about inability to gauge risks. However, the benefits have been noted by several of
us.
60;
Turnbuckle and I growing more hair, his being able to run 4 miles again after suffering
statin damage that made running 100 yards feel like he ran a marathon, scars fading for
both he and I, dogs that couldn't jump into a car's back seating finding it easy, my
wrinkles proven by photos to be 1/5th as deep or gone, chaps noting improvements in
lifting weights and such. There are a long list of anecdotal benefits, and not just by one
person, but by multiple people saying the same things.
60;
Then there are those who say they experience problems when they take C60oo every
day, who don't when they take it intermittently.
60;
And those, like me, who experience no problems other than I can't get buzzed from
alcohol.
60;
So far the anecdotal benefits seem to far outweigh reports of risks.

Yes, early on Turnbuckle, who remains one of the most intelligent contributors, noted that
the crown of his head that was bald was filling in with hair. He noted a study showing this
hair-growing effect 60;with rats.60;http://www.ncbi.nlm....pubmed/19223242
60;
As has happened several times, he said it and then I noticed it happening to me. My hair
appeared thicker and seemed to darken slightly over some months.
60;
Then he noted scars fading. I noticed the same, but also it was clear that wrinkles that
had begun to form around my eyes had faded. A photo of me from April, 2011 compared to
a photo of me in July, 2013 showed this clearly AND people who know me and hadn't seen
me in a while or people I just met said things like, "If you died your hair, you'd look 40." or
"You look younger than the last time I saw you." I'll be 61 in May. I simply don't have
much as far as wrinkles on my face.
60;
As a super-potent antioxidant, with something like 170 - 280 times more antioxidant
potential than vitamin C, C60 appears to enhance the "health" - maybe regenerative
ability of elastin and collagen in skin. If we can remove the damage of free-radicals, which
C60 appears to do, then skin elastin and collagen can regenerate without the assault of
free-radicals contributing to aging. Just a theory.

I wish that I had started taking it in my 20's. I believe that taking it at a younger age
would keep one younger than their peers going forward, by far.
60;
It doesn't solve all health concerns, but it certainly solves more than any other single
compound that I'm aware of.
60;
If a young person started taking it and adhered to an optimal diet with smart exercise it
seems likely that they might live incredibly long and healthy.
60;
Moussa said that they had passed a study design on to an American research team that
would keep giving the rats C60 indefinitely to see how long they live. Maybe they will
never die.
60;
One problem is that C60 doesn't resolve cardiovascular issues. It doesn't change blood
pressure, or lipids.
60;
So it might be anti-cancer, grow hair, reduce risk of Alzheimer's and other such anti-aging
effects, but both Turnbuckle and I have noted no seeming change in cardiovascular
parameters.
60;
So diet is important, because damage to the cardiovascular system is mostly caused by
what one consumes - or smokes.

* *

We've had a number of reports of remarkable improvements in old dogs from c60. I
suspect that mitochondrial dysfunction is a problem with old dogs, so c60 is just what the
doctor (vet?) ordered.
* *
In terms of testing on a younger small dog, I began giving weekly C60 to a mixed-breed
small dog that had a problem with her knee. The problem could only be truly fixed via
surgery but after a few weeks of C60 the limp has gone away and hasn't come back
(before it used to go away a few days then come back and repeat for the past year). It's
been months now which is the longest she's gone without limping in 18 months. We've
been increasing her ball-throwing times since she can handle more now.

* *
Just an update on the old dog mentioned in my previous post, quoted below.

It was given two 1 oz bottles of C60 Olive Oil over the course of about 3 months, and
then given no more. It's vitality has continued to improve, as would be expected
according to my hypothesis of one of the modes of action of the C60OO.

It was lying around and hardly moving when it first started getting the C60, and now it
runs around and gets into dogfights

The hypothesis, first stated --URL::6062ee04d94bf8f2538d5a2f96bc07b3-- here is that


C60OO reduces oxidation of proteins and thereby preserves their functionality. In the case
of activity, one major contributor is likely the 'ryanodine receptor', which is damaged in
older individuals, through oxidation. It would take some time for the damaged proteins to
be consumed and replaced with new ones.

Eventually more new undamaged proteins would be in place, and they would stay
relatively undamaged through the action of the C60. This would cause a gradual
improvement in activity level over time.

* *
What I have noticed are changes in endurance rather than speed. Used to be that solo
racquetball was something I could do only for a little while - I would have to stop and rest
frequently. With the C60 I don't need to stop, I can play until I'm bored with it. Don't
know if I'm any faster, but I definitely have more endurance.60;

* *
A few weeks ago I cam down on a rock while playing in my flag football league. I thought
that I had hurt my shoulder and was babying it, but I was still working the rest of my
body in the gym. I ended up damaging my bicep tendon, rather than my shoulder as I had
originally thought. A couple of weeks after the initialy injury, the stress on the tendon in
the gym caused it to snap, rupturing the long head bicep tendon compeletely. I was in
surgery 10 days ago to have the remaining tendon piece removed from my shoulder and
the bulk of the tendon reattached via bicep tenodesis, which reattaches it to the upper
part of the humerous. I had next to no swelling in my arm from the tendon rupture and far
less brusing than in any picture I saw. In addition, I was off pain meds compeletely 3 days
after surgery and my follow up today, 10 days after surgery, my surgeon was astonished
at how little swelling I had and how much range of motion I already had in my arm.
60;
C60 ? Who the hell knows. It sure didn't make the situation worse.
* *

Medical Professional..

Getting back to the subject of C60 anecdotes, I have experienced almost complete relief
of the excruciating pain in my left knee (I'm a candidate for a total knee replacement).60;
There is still a bit of crepitus when I move my knee, but no pain.60; I don't doubt the is
some antioxidant effect at work here, 60;but could C60 have an effect on the
Arachidonc Cascade and production of imflammatory prostaglandins?

I have been taking C60 from SV for almost 2 weeks now and have alternated between the
regimens advocated by Turnbuckle and niner.60; The results have exceeded my
expectations.60; Now I needn't destroy my heart with COX2 inhibitors or NSAIDS which
were some of the other alternatives offered me.60;

Thanks for all the helpful posts and possible mechanisms of actions that have been
offered on this website.60; I am extremely leery about ingesting uproven nutraceuticals
and the like.60; Again, this forum has proven most informative and helpful!

* *
Well here is a little more anecdotal stuff LOL

My knees started hurting right above the knee cap, when climbing ladders or squatting
down after about the age of 52-55, and when I started taking TA65 it went away for
awhile, but then came back. One thing I noticed with C60 was any problem with them
stopped, after about 4 hours of taking C60 and days later it would come back, and now it
seems to stay away when taking a 1/-1/2 teaspoon of C60 every 4 days or so. It is not
just your dog LOL

* *

Since none of the Wistar rats in the study died with cancerous tumors and normally all
Wistar rats do die with cancer, I would assume that C60oo would be highly protective of
cancer and I expect that I will never get cancer because I take 7 mg of C60oo every
morning.

It seems that one of the benefits that we might expect from C60oo is protection from
cancer...

I strongly suggest consideration of taking C60oo for it's anti-cancer effects.

Also, as Dr. Moussa said in his video interview, C60 is absolutely non-toxic, so I take it
assuming that it causes no cytotoxicity.

** *

My Roommate (the one with MS) has continued to notice improvements to her MS related
symptoms. Her balance has continued to improve at the same rate as her cardio
improvement. She hasn't burnt either (UV protection) and hasn't noticed and increased
sensitivity to Caffeine.

* *
can anyone explain to me why c60 has the same effect on me as taking anavar, 100
mg/day, but without the side effects? after three weeks of c60, my body is shredded to
the max and the increase in my physical strength is just ridiculous. i'm 32 years old, 186
cm tall and i weigh 81 kgs. thanks.

...IMLO, Many athletes have posted about having huge athletic gains while on C60, some
are even winning awards that were previously out of their reach.

You should have said that Anavar is an anabolic steroid. As for C60, the improved fat
tissue burning comes from enhanced mitochondrial function. I experienced the same.
About strength, it is more ATF produce. By the way Anavar is a mild steroid, so, it should
not have had side effects.

* *
Just OO effects:

Olive Oil
"The main factor responsible for aging, wrinkly skin is ultraviolet radiation, or skin
photoaging. A French study on 1,264 women and 1,655 men suggests that the
monounsaturated fatty acid in olive oil is protective against severe photoaging.
Photodamage, including blotchy pigmentation, wrinkling and skin sagging, is mainly caused
by the production of reactive oxygen species (ROS) in response to sun exposure. This
study suggests that olive oil in the diet increases the ability of the skin to resist damage
caused by UV radiation."
ABC

* *
I'll assert that as a 60 year-old who has taken 7 mg of C60oo every morning (except for a
week when I ran out) it has done nothing but good for me.
60;
Hair thicker, wrinkles 1/5th as deep, scars on my face have faded to invisibility.60;
I'm told, "If you died your hair, you'd look 40."60;
60;
I agree with Professor Fathi Moussa, the lead researcher of the study that showed nearly
double the lifespan of Wistar rats when he said that C60 is "absolutely not toxic" "even
taken long term" in the video interview that one can view on60;http://c60.net/60;
60;
He's been studying it for 18 years, so that makes me tend to believe him, as he is a world
authority.

* *
Well I took a big dose a week ago and I've taken a few smaller doses (3ml) since. Also
been on a faste that ended two days ago. I do feel invigorated, hard to say if it's
placebo. Definitely no problems with my endurance. My nose feels a bit drier than usual,
often it's runny but that might also be because summer is ending.

* *

I started taking C60 for the first time in about a year, one month ago. The most
noticeable improvement in my health I've noticed is a major reduction in the terribly
tendinitis that had developed in my left shoulder. I realize this improvement could be pure
cooncidence it would be pretty amazing if it is C60 that is primarily responsible. I've been
taking Sarah Vaughter's product if anyone is curious.

* *
Hi, I figured this should be reported here for what it's worth.

A male friend of a friend of mine, age 55 years, lives in a very remote village in Asia. For
over a year now, he's been suffering with chronic respiratory issues. It seems to have
been triggered by some heavy air pollution exposure on a trip to the city, which is unusual
in his rural environment. In any event, it continued to escalate gradually long after the
exposure had terminated. Perhaps his weakened immune system succumbed to a lung
infection or virus. About a month ago, it had gotten so bad that we thought he might die.
Due to his moderate smoking history, we thought it might be lung cancer. So despite the
difficulty in doing so, he found his way to a hospital where they took chest xrays.
Certainly, this is not the best way to diagnose lung cancer or other serious lung ailments
such as pneumonia. But anyone in his grave state of health should have had obvious
symptoms on an xray.

My friend told me about his predicament because she knows that I'm very interested in
leading edge health research of the sort that you find here on Longecity. I told her that
I'm not a doctor, and especially in the absence of a diagnosis and given his remote
location, this would be extremely difficult to help with.

Coincidentally, I had been doing some extensive c60oo research on this forum around this
time, a couple weeks ago. The most salient reported feature of c60oo is evidently that
improves endurance, particularly at peak performance. Peak performance was hardly
relevant here, but I chanced upon one posting in which the poster reported spirometric
evidence of increased lung capacity after some prolonged use. I doubt this was due to
the formation of new alveoli, unless that occurred as a natural response to increased
exercise ability. Given the large improvement, I suspect that most of the increase was due
to the clearance of excessive mucous, which was in turn the result of improved disease
resistance. In other words, the c60oo helped the poster's immune system function more
efficiently, which resulted in a reduction of inflammation and infective activity. Either way,
the anecdote was moderately compelling, particularly since I don't recall other posts
reporting the same.

Based on the c60oo retailer review thread, I selected carbon60oliveoil.com (not to be


confused with c60oliveoil.com).... I took a gamble and ordered some. I knew it would cost
a lot to get it to him, but what's a couple hundred bucks to maybe give him another year
of life or more? If it fails, then at least we'll have some other potentially illuminating data.

Let me be clear. We still don't know what he has. My best guess at this point would be a
chronic lung infection that has taken advantage of a depressed immune system. He has
been taking a daily multivitamin of known good origin for over a year, and more recently a
variety of natural herbs. I suggested that he add turmeric and mushrooms to that
regimen. All in all, they seem to have stabilized him as of a week ago, but he was not
improving. I told him that I couldn't promise to help him, and that in theory c60oo might
even hurt him, although the weight of the evidence thus far was strongly against that
possibility.

. I told him to take a level teaspoon per day, with a sip of water before and after
(because one poster suggested that, and it makes sense because you don't want it
sitting in the esophagus), in the morning before eating anything else. (You can imagine
the challenges of trying to get a syringe though customs, so "eyeball" measurement will
have to do.) As I understand it, this should be roughly 3 mg per day. I told him to take
this daily until the supply was exhausted, perhaps in a month.

I told him was that there was "some evidence" that it might help with lung problems,
particularly lung capacity. And of course the warning that this stuff, although well tested,
was not a proven treatment for anything, and its side effects are not presently
understood. I also said that it would be at least a week before he noticed anything, if
indeed he would notice anything at all. I also warned him that if he found himself to have
great endurance, that he should nevertheless exercise only modestly, and get 20 minutes
of sun exposure daily, but otherwise take it easy and allow himself to recover. I realize
that most users have reported effects with a few days, but given his condition, that
wasn't in the cards here, and I certainly didn't want to give him false hope.

He took his first dose while on the phone, and said it had a nice oily taste, but otherwise
noticed nothing. Now it's a couple days later, and he has consumed 2 doses. Not much,
statistically, except that this is a highish dose, considering that 1.5 mg seems to be
something of a maintenance level, and he's of average build and below average height for
his age, vs. most Westerners. My friend called him today because I told her to ask about
any bad reactions he might have experienced, knowing that it was too early to discuss
positive changes.

His only complaint was that he had developed an ache in his upper back since taking it.
When she asked why, he said he wasn't sure. But he went on to explain that he was
sleeping on his back, which is something that he had not been able to do since his disease
had worsened (and might explain the backache, due to atrophied muscles trying to
maintain this posture). He said he was sleeping much better because he could breathe
better (which seems to explain why he could once again sleep on his back). He also said
that he no longer felt tired, which was unexpected, because he hadn't been told to
expect anything in this regard. I honestly didn't expect this myself, as I thought c60oo
was mostly about endurance, not basic wakefulness. Maybe if you're sick, it makes you
less tired, and when you're healthy, it makes you more endurant. Anyway, the hope is
that this thing is some sort of infection which we might be able to kill by temporarily
revving his cellular efficiency.

......another update on my friend's friend once he finishes his c60oo, probably later this
month. He says he's "fine" now except for a sore throat (pathogen or olive oil burns, not
sure which), but we didn't a chance to determine what "fine" actually means, on account
of a bad connection.

* *
Can definitely confirm that it has almost eliminated dental tartar/plaque. I normally have
visit the dental hygenist every 3 months (nearly everyone in my family has to do this too
- it seems to run in our family). I have been traveling and really busy so it had been 7
months. And my teeth looked much better than they would have normally at 3 months.

Also, I've noticed that when I wake up in the morning my breath smells kind of nice -
which, is somewhat unusual.....lol :D
* *

Cells, whether permanent (these "cells are rarely or not at all replaced during life and can
be as old as the whole organism") or not, must be cleansed of toxins in order to maintain
their enthusiam for keeping you alive. What is different about C60 is that it ultilizes a very
basic building block of life, the Carbon molecule. Carbon filters have been around for a long
time... it seems intuitively correct that this substance could play a part in cleasing our
cells. I cannot say for sure at this point if Bucky's are making me younger, but I feel it is
worthwhile to take it and I will continue to do so. We have two older pets... a cat and a
small dog, both around 14-years old. Whenever they seem to be showing their age, I put
some C60 in their food. Both of them immediately start to behave like very young animals,
playful, active, bright-eyed. Is that conclusive proof? Of course not, but it sure is
encouraging as nothing else ever did that for them before.
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16th July 2017 11:34 Link to Post #3

Daozen
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
Thanks for posting this stuff. Very useful.
Avalon Member
I'll assert that as a 60 year-old who has taken 7 mg of C60oo every morning (except
for a week when I ran out) it has done nothing but good for me.
60;
Hair thicker, wrinkles 1/5th as deep, scars on my face have faded to invisibility.60;
I'm told, "If you died your hair, you'd look 40."60;
60;
I agree with Professor Fathi Moussa, the lead researcher of the study that showed nearly
double the lifespan of Wistar rats when he said that C60 is "absolutely not toxic" "even
taken long term" in the video interview that one can view on60;http://c60.net/60;
60;
He's been studying it for 18 years, so that makes me tend to believe him, as he is a world
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authority.
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Age: 39
**
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Well I took a big dose a week ago and I've taken a few smaller doses (3ml) since. Also
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been on a faste that ended two days ago. I do feel invigorated, hard to say if it's placebo.
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Definitely no problems with my endurance. My nose feels a bit drier than usual, often it's
runny but that might also be because summer is ending.

Looks like it might cost about 5USD per week.


Parent Post

Last edited by Daozen; 16th July 2017 at 11:49.

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16th July 2017 13:17 Link to Post #4

Rawhide68
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
"C60 will kill Ebola virus, a Stem Cell Scientist stated"
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Damn that rocks! It will cure me from ebola, finaly !
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Last edited by Rawhide68; 16th July 2017 at 13:57.

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16th July 2017 14:38 Link to Post #5


EFO
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !

Avalon Member and another in Switzerland--

http://shop.owndoc.com/p-203/c60-oli...ls-in-oil.html

The original posted link gave me


"Not Found

Join Date: 13th May 2016 The requested URL /p-203/c60-olive-oil-buckminsterfullerene-buckyballs-in-oil.html was
Age: 48 not found on this server.
Posts: 731
Thanks: 3,401 Additionally, a 404 Not Found error was encountered while trying to use an ErrorDocument
Thanked 3,510 times in 676 posts to handle the request."
here is the full text link (add h before ttp://):
ttp://shop.owndoc.com/product-info.php?c60-olive-oil-buckminsterfullerene-buckyballs-
in-oil-pid203.html

http://shop.owndoc.com/product-info....il-pid203.html
Parent Post

"Your planet is forbidden for an open visit - extremely aggressive social environment,despite almost
perfect climatic conditions.Almost 4 billion violent deaths for the last 5000 years and about 15000 major
military conflicts in the same period."

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16th July 2017 15:18 Link to Post #6

Basho
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
Thank you so much for this thread. I was just now reading your other thread & was going
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to order some c60 today, so these detailed posts are the icing on the cake.
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Location: Here, now I've also researched & cured various serious ailments by fanatical reading & experimenting
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Thanked 455 times in 109 posts cannot be reversed so rapidly. But taking responsibility for my health & being proactive
with treatments, has bought me extra years of life & increased my quality of life. Have
had multiple friends & acquaintances die of heart attacks in the past 6 months(46 same
age as myself), so I'm at that fork in life where health is of utmost importance.
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ISness is my business..

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17th July 2017 01:06 Link to Post #7

Perdido
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
Notes were from 2 years ago when I was researching this.. so there maybe broken links..
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17th July 2017 01:23 Link to Post #8

Daozen
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
Here's a source for the pure powder. The price drops sharply when you spend 200USD or
Avalon Member
more.

https://us.vwr.com/store/product/748...owder-99-5-c60

Sigma Aldrich's looks cheaper.

http://www.sigmaaldrich.com/catalog/...g=en&region=TW

These guys are cheaper. No idea about quality.

https://www.sesres.com/fullerene/
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Last edited by Daozen; 17th July 2017 at 01:34.

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17th July 2017 01:47 Link to Post #9

Perdido
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
It is a matter of making the powder absorbent by the body... not the price of the raw
Avalon Member
material.
Join Date: 21st July 2016
Location: Pensacola, FL I suggest just trying the product first to see if it benefit you. You can focus on producing
Posts: 47 it later, if it works.
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17th July 2017 02:10 Link to Post #10

Daozen
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
I understand people have to do their own research... but most of us coming to this thread
Avalon Member
are looking for the following info:
- Where can I buy this?
- How much does a daily dose cost?
- What's the best form to take this?
- What are the benefits and tradeoffs between buying it pre-prepared in Olive oil, and
buying the powder myself.?

I'm digging thorugh your post to find the relevant information.


Parent Post

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Last edited by Daozen; 18th July 2017 at 00:31.

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17th July 2017 07:01 Link to Post #11

devplan
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !

Avalon Member Posted by Daozen (here)


I understand people have to do their own research... but most of us coming to this thread
are looking for the following info:

- Where can I buy this?


- How much does a daily dose cost?
- What's the best form to take this?
- What are the benefits and tradeoffs between buying it pre-prepared in Olive oil, and
Join Date: 17th April 2015
buying the powder myself.?
Location: Reinbek, Germany
Posts: 116
I'm digging thorugh your info to find the relavent information.
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@Daozen

Most of your questions I saw being addressed at the Swiss shop link product page:
http://shop.owndoc.com/product-info....il-pid203.html

Parent Post

"The greatest good you can do for another is not just share your riches, but to reveal to him his own."
-- Benjamin Disraeli

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17th July 2017 11:16 Link to Post #12

Daozen
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
Avalon Member
Posted by devplan (here)
@Daozen

Most of your questions I saw being addressed at the Swiss shop link product page:
http://shop.owndoc.com/product-info....il-pid203.html

You're right devplan, thanks for that. 20 Euros for a months supply seems like a good
start. I'll buy a bottle ASAP. My parents will probably get one too. Cheers.

Join Date: 16th February 2012 45 mg Buckminsterfullerene in 50 ml in extra virgin olive oil. This is a higher
Location: Dark Alliance HQ concentration (the maximum attainable at room temperature) than available from other
Age: 39 vendors, because we use overhead stirrers instead of magnetic stirrers and we micro-
Posts: 1,751 pulverize the C60 first.
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Thanked 7,647 times in 1,512 posts Recommended dose 1.5 mg/day, which is a third of a teaspoon. The bottle should last 1
month. It is unknown what the optimal dose is, so exact dosing is not important.

Our C60 comes from Solaris Chem in Canada.

CANADIAN CUSTOMERS: Please no order more than 4 bottles or your customs may send
them back.

This product is prepared exactly the same as the product used in the famous rat longevity
study where "Bucky" rats lived nearly twice as long as the control group. C60 is clinically
proven to have many beneficial health effects in a variety of organisms. It has anti-cancer
effects, brain-rejuvenating effects and cardiovascular health effects as well as extremely
dramatic longevity effects in a variety of organisms. For more information on these and
other proven biological effects, see c60antiaging.com.

99.5% C60 from Solaris in Canada, vacuum-over dried to > 99.95% in our lab. Extra Virgin
Olive oil pressed in our presence in the Cretan village of Voulgaro and transported to our
production lab in Swedish Lapland in Västerbotten.

Recommended daily dose for a large adult primate 1.5 mg/day, every day, making a
bottle last 30 days.

Large Adult Primate?


Parent Post

Last edited by Daozen; 17th July 2017 at 11:19.

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17th July 2017 12:04 Link to Post #13

Feritciva
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !

Avalon Member
Join Date: 29th January 2011 @Daozen
Location: Planet Earth. Possibly
for too long. Most of your questions I saw being addressed at the Swiss shop link product page:
Age: 40 http://shop.owndoc.com/product-info....il-pid203.html
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This link does not to work, it gives dns error. I'm also interested in how to buy this
supplement. Anybody have any other sources?
Parent Post

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17th July 2017 12:10 Link to Post #14

Daozen
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
The link's working from here.
Avalon Member
http://shop.owndoc.com/product-info....il-pid203.html
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17th July 2017 12:12 Link to Post #15

Flash
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
Avalon Member
You have been given full of sources in the first snd second post. 4 of the 5 links are
working. For you Swiss must be the easiest. And not more expensive if comparing apples
with apples , meaning grams of c60 and ml of oil, with other suppliers. I personnally prefer
the quality of any swiss product usually over american ones. swiss have a long tradition of
health products and of bio discoveries when taking into account that they are only 8
millions of population.

Swiss universities are also at the forefront in similar newly developed compounds. They
have a ready market to supply (joking about the international bankers eternal life desires if
they are not suicided)

Posted by Feritciva (here)


Join Date: 26th December 2010
Location: Montreal
@Daozen
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Most of your questions I saw being addressed at the Swiss shop link product
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page:
http://shop.owndoc.com/product-info....il-pid203.html

This link does not to work, it gives dns error. I'm also interested in how to buy this
supplement. Anybody have any other sources?

Parent Post

Last edited by Flash; 17th July 2017 at 12:19.


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17th July 2017 18:04 Link to Post #16

CurEus
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
I've read a lot on C60 this morning. Does anyone have any personal experiences or
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knowledge of this they would like to share?
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17th July 2017 22:07 Link to Post #17

Perdido
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
As I previously posted in another thread, and probably should have posted here initially, I
Avalon Member
have taken Carbon 60 for 2 years.
Join Date: 21st July 2016
Location: Pensacola, FL I double dosed the first day and felt nothing during the day, but on awakening.. I felt like
Posts: 47 a 16 year old kid! I felt so good that I just laid there breathing and enjoying the
Thanks: 68 sensation. At this time in my life I had more pain and physical problem, like with sleep, so
Thanked 209 times in 44 posts being refreshed after sleeping was great. To this day, even if I just take a nap, I feel
wonderful on awakening. I also enjoy just laying there breathing and enjoying the pleasant
sensation.

Initially I took 1/4 tsp of C60 daily for the first bottle. Now I will take 1/4-1/2 tsp
anywhere from 3 days a week, to every couple of weeks just to keep it in my system. If I
was ill or exercising hard, I would take it daily.

A friend with long term Leukemia took it and said he noticed he felt better every day.

Initially I ordered from the Swiss supplier, but now use Carbon60OliveOil.com It is faster
for me mainly. I did not notice any difference in the two products.

My German Shepherds have taken it when they are ill especially. The female took it right
before she had pups, and immediately afterwards to help her recover.
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18th July 2017 03:23 Link to Post #18

CurEus
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
It was mentioned by one user that it seems very like the effects of Gold Mormus...
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Interesting to note that Russia's very first natural healing baths/spas were built in areas
Join Date: 2nd June 2010 with naturally occurring C60 in the water/rocks. I may give this a try! Thanks for the info!
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18th July 2017 04:55 Link to Post #19

kanishk
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
What about the Buckyballs of Silicon created by Dr. Patrick Flanagan, who claims that it
Avalon Member
decreases the surface tension of water and that such Buckyballs were naturally present in
the water of Hunza Valley; and therefore the people of Hunza Valley used to live much
longer and look younger at very old age.
Parent Post

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18th July 2017 09:08 Link to Post #20

kanishk
Re: Carbon 60 (C60) Antioxidant that works at the DNA Level !
Fullerence C60 is available on Alibaba from China. CAS NUMBER: 99685-96-8
Avalon Member
I asked them for Quotations, they sent me this message.

We supply the C60 powder as below:


General type, without functional group.
Spec is diameter: 0.7nm, Length: 1.1nm with purity of 99.9%.
We also produce Soluble C60, including water-soluble and alcohol-soluble.
Would you please confirm the type you need?
CAS number for this product is 99685-96-8

Why a Bucky ball has a different length and diameter? What type of C60 is used in making
Join Date: 24th September 2011 these C60 olive oil products? Is the a patent somewhere so I can look into?
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95% purity 1 gram is 24 USD.
Without shipping cost.
Parent Post

Last edited by kanishk; 18th July 2017 at 09:51.

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