Professional Documents
Culture Documents
1
Examples
2
Cuts of poultry
3
Amyloidosis
Introduction
A Coronavirus infection of chickens with a
morbidity of 50-100% and a mortality 0-25%,
depending on secondary infections. Infection is
via the conjunctiva or upper respiratory tract
with an incubation period of 18-36 hours. The
infection spreads rapidly by contact, fomites or
aerosol. Some birds/viral strains can be carriers
for up to 1 year. The virus, which may survive 4
weeks in premises, is sensitive to solvents, heat
(56°C for 15 mins), alkalis, disinfectants
(Formal 1% for 3 mins). Poor ventilation and
high density are predisposing factors.
Signs
• Sudden death.
• Muscular shivering.
• Otherwise as for standard IB.
Post-mortem lesions
• Oedema of pectoral muscles and
subcutaneously on abdomen, lesions
4
progress to necrosis and scarring of deep
pectorals in convalescence.
• In layers the ovules may be intensely
congested.
• Other lesions of 'classical' IB may be
encountered.
Diagnosis
3-5 passages in CE allantoic cavity, HA-, typical
lesions, FA, ciliostatic in tracheal organ culture,
cell culture (Vero, CK) only after adaptation
Serology: HI, Elisa (both group specific), SN
(type specific), DID (poor sensitivity, short
duration, group specific).
Treatment
Sodium salicylate 1gm/litre (acute phase) where
permitted - antibiotics to control secondary
colibacillosis (q.v.).
Prevention
Live vaccines of appropriate sero-type and
attenuation, possible reactions depending on
virulence and particle size.
5
Anatipestifer Disease, New Duck
Syndrome, Duck Sepicaemia
Introduction
An acute or chronic septicaemic disease caused by
Riemerella anatipestifer, syn Pasteurella, or Moraxella a. It
affects ducks of any age, sometimes turkeys, and may also
be isolated from chickens, game birds and wild waterfowl.
Mortality is 2-75% in young ducks. Transmission is mainly
direct, bird-to-bird, via toenail scratches, especially of the
duckling foot, or through respiratory epithelium during
respiratory disease. It can also be by faecal contamination
of feed, water or the environment where survival of the
infectious agent may be prolonged. Adverse environmental
conditions and pre-existing disease are predisposing
factors.
Signs
• Weakness.
• Neck tucked in.
• Head/neck tremor.
6
• Ataxia.
• Disinclined to walk.
• Incoordination.
• Dyspnoea.
• Ocular and/or nasal discharge.
• Hyperexcitability
Post-mortem lesions
• Perihepatitis without much smell or liver damage.
• Pericarditis.
• Airsacculitis.
• Enlarged liver and spleen.
• Occasionally fibrinous meningitis.
• Salpingitis
• Purulent synovitis.
• Chronic arthritis, sometimes with erosions of the joint
cartilage.
Diagnosis
Lesions, isolation and identification of organism - blood or
chocolate agar in candle jar or 5% CO2. Differentiate from
duck viral enteritis, duck viral hepatitis, fowl cholera,
colibacillosis, coccidiosis, chlamydiosis.
Treatment
Sulphonamides and potentiated sulphonamides are the
products most commonly recommended for drinking water
application. Subcutaneous injections of penicillin +
7
dihydrostreptomycin, or streptomycin +
dihydrostreptomycin are also highly effective.
Prevention
Good husbandry and hygiene, rigid depopulation and
disinfection, adequate protection, 'hardening off', correct
house relative humidity, sulphonamides in feed. Inactivated
and attenuated vaccines available in some countries.
Autogenous bacterins sometimes used.