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MULTIPLE EXTRACTION OF CAFFEINE FROM LIPTON BLACK TEA LEAVES BY

USE OF DICHLOROMETHANE

Padilla D. Alyssa, Pang Khrystine, Phillips M. Xyra, Sangalang A. Kyle, Sarrosa L. Isabelle
and Supe S. Mikhael

Group 6 2A Pharmacy Organic Chemistry Laboratory

ABSTRACT

Caffeine was isolated through the use of the multiple extraction method. 10 g of tea leaves
along with 100 mL of distilled water in a 500 mL beaker was boiled. The aqueous solution
was extracted three times using 20 mL of dichloromethane each time. The organic layer
containing dichloromethane was obtained by use of filter paper containing anhydrous
sodium sulfate into an evaporating dish and covering it with filter paper that has small holes
to evaporate to dryness. The percentage recovery computed was 0.2% by getting the ratio
of the weight of caffeine and the weight of the tea leaves used and multiplying it by a
hundred.

INTRODUCTION separatory funnel, beaker, glass rod,


evaporating dish, iron stand, iron ring,
Caffeine is a substance or compound iron clamp, teabags, sodium carbonate,
that acts as a stimulant and can be found dichloromethane, and sodium sulfate.
naturally in tea, coffee, chocolate, kola
nuts in cola, and is added to carbonated First, in a 500 milliliter beaker, the
drinks, food, and medicines. researchers placed 4.4 grams of
anhydrous sodium carbonate and 100
Extraction is a method of separating a milliliters of distilled water and heated in a
certain substance from a mixture. It uses water bath until the solid dissolved. When
two immiscible phases to separate the the solid (anhydrous sodium carbonate)
substance from one phase into the other. dissolved, 10 grams of tea leaves from tea
There are two types of extraction: single bags (approximately 5 tea bags) was
and multiple extraction. The type of added to the said mixture. The flask was
extraction used for this study was Multiple covered and the tea mixture was boiled
Extraction. for 10 minutes on a low flame.
Afterwards, the cheesecloth was removed
The objectives of this experiment are to and the liquid was squeezed out by
isolate the caffeine from the dried tea pressing the stirring rod against the
leaves through the method of multiple cheese cloth. The extract was cooled down
extraction and record the weight of the until it reached room temperature.
used dried tea leaves to compute for the
percentage recovery. Figure 1.0

EXPERIMENTAL

A. Test Compound/s (or


Sample/s) used

10 grams of Lipton Black tea leaves

B. Procedure

The materials needed for the


experiment were: Bunsen burner,
For the multiple extraction proper, the
tea mixture was transferred to a
separatory funnel. 20 milliliters of
dichloromethane was added to the tea
mixture and the contents of the funnel
were swirled slowly. The method was
done for approximately 2 minutes until
the separation between the two layers
became clearly visible. The
dichloromethane was drained out of the
funnel and placed in an evaporating dish.
This was done three times because the
method used was multiple extraction.

Figure 1.2

After the multiple extraction proper, the


organic layer (dichloromethane layer) was
filtered into the evaporating dish using
filter paper containing half a spatula of
anhydrous sodium sulfate. Then, the
evaporating dish containing the extract
was covered with filter paper that had
small holes to evaporate

RESULTS

In order to calculate for the percentage


recovery, the researchers recorded the
weight of the following

Figure 1.3 Table 1. Data needed in order to


calculate for the percentage recovery

WEIGHT OF grams
Tea leaves used 10.02 g
Evaporating dish + 116.50 g
caffeine
Empty evaporating 116. 48 g
dish
caffeine 0.02 g

By using the values above, the percentage


recovery of 0.2% was computed by using
the formula:

𝑤𝑒𝑖𝑔ℎ𝑡 𝑜𝑓 𝑐𝑎𝑓𝑓𝑒𝑖𝑛𝑒
𝑥 100 = % 𝑟𝑒𝑐𝑜𝑣𝑒𝑟𝑦
𝑤𝑒𝑖𝑔ℎ𝑡 𝑜𝑓 𝑡𝑒𝑎 𝑙𝑒𝑎𝑣𝑒𝑠 𝑢𝑠𝑒𝑑

0.02 𝑔
𝑥 100 = 0.2%
10.02 𝑔

Figure 1.4
DISCUSSION [5] Extraction – Organic Lab (2013,
October 6). Retrieved October 1, 2018,
The technique of using dichloromethane from
for extracting caffeine from tea leaves was https://lehighcheme.wordpress.com/2013
proven to be efficient. The fact that /10/16/extraction-organic-lab/
caffeine is more soluble in
dichloromethane (140mg/ml) than in From the internet
water (22 mg/ml) proves that
dichloromethane is very effective at [1] Caffeine in Lipton Tea. (n.d.).
extracting caffeine. Retrieved October 2, 2018, from
https://www.caffeineinformer.com/caffein
The errors made during the experiment e-content/lipton-tea
were spillage, loss of liquid from
transferring, stain of liquid on the [2] Definition of Caffeine. (n.d.).
cheesecloth, and bubbles. The tea extract Retrieved September 29, 2018, from
spilled during the transfer of liquid from https://www.medicinenet.com/script/main
the beaker to another beaker and some of /art.asp?articlekey=11068
the liquid were left on the original beaker
[3] Definition of Extraction. (n.d.).
which contribute to percentage loss.
Retrieved October 1, 2018, from
Another factor is the stain of liquid on the
https://www.chemicool.com/definition/ext
cheesecloth. When the researchers were
raction.html
squeezing out the liquid from the tea
leaves in the cheese cloth, it left a huge [4] Extraction of Caffeine from Tea.
stain on the cheese cloth, therefore (2011). Retrieved October 1, 2018, from
affecting percentage loss again. The http://vlab.amrita.edu/?sub=3&brch=64&
bubbles at the bottom of the funnel during sim=169&cnt=1
the swirling of the funnel also contributes
to percentage loss. The “bubbles” [5] Extraction – Organic Lab (2013,
mentioned is an emulsion, which happens October 6). Retrieved October 1, 2018,
when a liquid is dissolved in another from
liquid, in this case it is water and DCM. https://lehighcheme.wordpress.com/2013
/10/16/extraction-organic-lab/
REFERENCES

[1] Caffeine in Lipton Tea. (n.d.).


Retrieved October 2, 2018, from
https://www.caffeineinformer.com/caffein
e-content/lipton-tea

[2] Definition of Caffeine. (n.d.).


Retrieved September 29, 2018, from
https://www.medicinenet.com/script/main
/art.asp?articlekey=11068

[3] Definition of Extraction. (n.d.).


Retrieved October 1, 2018, from
https://www.chemicool.com/definition/ext
raction.html

[4] Extraction of Caffeine from Tea.


(2011). Retrieved October 1, 2018, from
http://vlab.amrita.edu/?sub=3&brch=64&
sim=169&cnt=1