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  • False Reactions With RH Typing Reagents False-Positives False-Negatives

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    Aaron James Ruedas
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    This document lists potential causes of false positive and false negative results with Rh typing reagents and recommended actions. False positives may occur due to issues like a heavy cell suspension, cold agglutinins, or long incubation time. False negatives can be caused by immunoglobulin-coated cells, failure to follow directions, or reagent deterioration. The recommendations are to adjust procedures, use alternative reagents, or repeat the test following manufacturer guidelines.

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    False Reactions with Rh Typing reagents

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    Rh typing reagent

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    This document lists potential causes of false positive and false negative results with Rh typing reagents and recommended actions. False positives may occur due to issues like a heavy cell suspension, cold agglutinins, or long incubation time. False negatives can be caused by immunoglobulin-coated cells, failure to follow directions, or reagent deterioration. The recommendations are to adjust procedures, use alternative reagents, or repeat the test following manufacturer guidelines.

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    © All Rights Reserved
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    94 views2 pages

    False Reactions With RH Typing Reagents False-Positives False-Negatives

    Uploaded by

    Aaron James Ruedas
    This document lists potential causes of false positive and false negative results with Rh typing reagents and recommended actions. False positives may occur due to issues like a heavy cell suspension, cold agglutinins, or long incubation time. False negatives can be caused by immunoglobulin-coated cells, failure to follow directions, or reagent deterioration. The recommendations are to adjust procedures, use alternative reagents, or repeat the test following manufacturer guidelines.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    of 2

    False Reactions With Rh Typing Reagents

    FALSE-POSITIVES FALSE-NEGATIVES
    1. Cell suspension too 1. Adjust suspension, 1. Immunoglobulin-coated 1. Use saline-active typing
    heavy retype cells (in vivo) reagent

    2. Cold agglutinins 2. Wash with warm saline, 2. Saline-suspended cells 2. Use unwashed cells
    retype (slide)

    3. Test incubated too long 3. Follow manufacturer’s 3. Failure to follow 3. Review directions;
    or drying (slide) instructions precisely manufacturer’s directions repeat test
    precisely

    4. Rouleaux 4. Use saline-washed cells, 4. Omission of reagent 4. Always add reagent first
    retype manufacturer’s directions and check
    before adding cells

    5. Fibrin interference 5. Use saline-washed cells, 5. Vigorous shaking during 5. Resuspend all tube tests
    retype resuspension gently

    6. Containing low- 6. Try another 6. Incorrect reagent 6. Read vial label carefully;
    incidence antibody in manufacturer’s selected repeat
    reagent reagent or use a known
    serum
    antibody
    7. Polyagglutination 7. Variant antigen 7. Refer sample for further
    investigation

    8. Bacterial contamination 8. Open new vial of 8. Reagent deterioration 8. Open new vial
    of reagent vial reagent,
    retype
    9. Incorrect reagent 9. Repeat test; read vial 9. Centrifugation too short 9. Repeat test using longer
    selected label carefully centrifugation time

    10. Centrifugation too long 10. Repeat test using 10. rpm too low 10. Repeat testing using
    shorter higher rpm
    centrifugation time
    11. rpm too high 11. Repeat test using
    lower rpm

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