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False Reactions With Rh Typing Reagents

FALSE-POSITIVES FALSE-NEGATIVES
1. Cell suspension too 1. Adjust suspension, 1. Immunoglobulin-coated 1. Use saline-active typing
heavy retype cells (in vivo) reagent

2. Cold agglutinins 2. Wash with warm saline, 2. Saline-suspended cells 2. Use unwashed cells
retype (slide)

3. Test incubated too long 3. Follow manufacturer’s 3. Failure to follow 3. Review directions;
or drying (slide) instructions precisely manufacturer’s directions repeat test
precisely

4. Rouleaux 4. Use saline-washed cells, 4. Omission of reagent 4. Always add reagent first
retype manufacturer’s directions and check
before adding cells

5. Fibrin interference 5. Use saline-washed cells, 5. Vigorous shaking during 5. Resuspend all tube tests
retype resuspension gently

6. Containing low- 6. Try another 6. Incorrect reagent 6. Read vial label carefully;
incidence antibody in manufacturer’s selected repeat
reagent reagent or use a known
serum
antibody
7. Polyagglutination 7. Variant antigen 7. Refer sample for further
investigation

8. Bacterial contamination 8. Open new vial of 8. Reagent deterioration 8. Open new vial
of reagent vial reagent,
retype
9. Incorrect reagent 9. Repeat test; read vial 9. Centrifugation too short 9. Repeat test using longer
selected label carefully centrifugation time

10. Centrifugation too long 10. Repeat test using 10. rpm too low 10. Repeat testing using
shorter higher rpm
centrifugation time
11. rpm too high 11. Repeat test using
lower rpm