Male-to-Male Sexual Transmission of Zika Virus — Texas, January 2016 | MMWR 18/10/18 11&39

Male-to-Male Sexual Transmission of Zika Virus — Texas, January 2016

Weekly / April 15, 2016 / 65(14);372–374

D. Trew Deckard, PA-C1; Wendy M. Chung, MD2; John T. Brooks, MD3; Jessica C. Smith, MPH2; Senait Woldai, MPH2;
Article Metrics
Morgan Hennessey, DVM4,5; Natalie Kwit, DVM4,5; Paul Mead, MD4 (View author affiliations)

View suggested citation Altmetric:

Zika virus infection has been linked to increased risk for Guillain-Barré syndrome and adverse fetal outcomes,
including congenital microcephaly. In January 2016, after notification from a local health care provider, an
investigation by Dallas County Health and Human Services (DCHHS) identified a case of sexual transmission of Zika (https://www.altmetric.com/details.php?
virus between a man with recent travel to an area of active Zika virus transmission (patient A) and his nontraveling domain=www.cdc.gov&citation_id=671658
News (62) (https://www.altmetric.com/details.ph
male partner (patient B). At this time, there had been one prior case report of sexual transmission of Zika virus (1). The domain=www.cdc.gov&citation_id=6716586&tab
present case report indicates Zika virus can be transmitted through anal sex, as well as vaginal sex. Identification and Blogs (7) (https://www.altmetric.com/details.php
domain=www.cdc.gov&citation_id=6716586&tab
investigation of cases of sexual transmission of Zika virus in nonendemic areas present valuable opportunities to
Policy documents (4)
inform recommendations to prevent sexual transmission of Zika virus. (https://www.altmetric.com/details.php?
domain=www.cdc.gov&citation_id=6716586&tab
Epidemiologic Investigation documents)
Twitter (223) (https://www.altmetric.com/details.p
In January 2016, 2 days after returning to Dallas, Texas, from a 1-week visit to Venezuela, patient A developed domain=www.cdc.gov&citation_id=6716586&tab
Facebook (15) (https://www.altmetric.com/detail
subjective fever, pruritic rash on his upper body and face, and conjunctivitis lasting 3 days. Both 1 day before and 1 day
domain=www.cdc.gov&citation_id=6716586&tab
after his symptom onset (Day 0), patient A had condomless insertive anal sex with patient B. Patient A reported that Google+ (1) (https://www.altmetric.com/details.p
during and after illness he experienced no symptoms of prostatitis or dysuria, and noted no macroscopic domain=www.cdc.gov&citation_id=6716586&tab
Reddit (3) (https://www.altmetric.com/details.php
hematospermia. domain=www.cdc.gov&citation_id=6716586&tab

On Day 7, patient B developed a subjective fever, myalgia, headache, lethargy, and malaise; a few days later, he Mendeley (176)
developed a slightly pruritic rash on his torso and arms, small joint arthritis of his hands and feet, and conjunctivitis. All
symptoms resolved after 1 week. On Day 11, while still symptomatic, patient B visited his primary care provider for Citations:
evaluation. Suspecting Zika virus infection, the provider obtained serum specimens from patient B on Day 11 (4 days
Views equals page views
after patient B’s illness onset), and from both patients A and B on Day 14 (14 and 7 days after respective illness Views: plus PDF downloads

onsets). On Day 24, semen, urine, and saliva specimens were collected from both patients (24 and 17 days after
respective illness onsets). Metric Details

Patient A had traveled regularly to Central and South America for many years. During his recent trip to ∠ Top
Summary
Venezuela, he reported that multiple persons in the area he visited were experiencing symptoms consistent with Zika
What is already known about
virus disease; autochthonous transmission of Zika virus had been confirmed in Venezuela in late November 2015.*
this topic?
Patient B had not recently traveled outside of the United States and had never traveled to countries with active
autochthonous Zika transmission. Neither patient had a history of prior known arboviral infection nor had they Although Zika virus is spread
received yellow fever or Japanese encephalitis vaccinations. The men had been mutually monogamous for more than primarily by Aedes species
10 years and had no major medical illnesses or history of sexually transmitted infections. Neither patient reported mosquitoes, published case reports
ulcerative anal or genital lesions. have documented sexual
transmission from infected men to
Laboratory Investigation ∠ Top
their female sex partners through
Samples of all clinical specimens were sent by DCHHS to CDC. Patient A’s serum from 14 days after illness onset and vaginal sex.
patient B’s serum from 4 days after illness onset contained no detectable Zika virus RNA using reverse transcription What is added by this report?
polymerase chain reaction (RT-PCR) testing (Table) (2). Sera from both patients demonstrated positive
immunoglobulin M (IgM) responses by capture ELISA for Zika virus and dengue virus, but not for chikungunya virus This is the first report of

(Table) (2). Plaque-reduction neutralization tests (3) indicated that patient A had been infected with Zika virus, dengue transmission of Zika virus from an

virus serotype 1, or both, but that patient B had been infected only with Zika virus. Urine and saliva specimens infected man to a sex partner

collected from patients A and B at 24 and 17 days after respective illness onsets had no detectable Zika virus by RT- through anal sex.

PCR. What are the implications for

Semen specimens collected at 24 and 17 days from each man were tested for Zika virus by RT-PCR both by CDC and public health practice?
DCHHS using the same two sets of primers (2). At CDC, neither sample had detectable Zika virus with either primer Sexual transmission through both
set after 37 cycles. At DCHHS, which pretreated the thawed semen samples with dithiothreitol (used to induce vaginal and anal sex is an emerging
mode of Zika virus infection that

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Male-to-Male Sexual Transmission of Zika Virus — Texas, January 2016 | MMWR 18/10/18 11&39

liquefaction of viscous specimens and potentially increase detection of RT-PCR targets), patient B’s specimen was might contribute to more illness
negative. Patient A’s specimen had Zika virus detected at 35 cycles with one primer set but produced no signal after 37 than was anticipated when the
cycles with the other primer set. Patient A’s semen results were thus deemed equivocal. outbreak was first recognized. Cases
of sexually transmitted Zika virus
Environmental Investigation ∠ Top
infection should be reported to
Although Dallas is within the geographic range of the Zika virus mosquito vectors Aedes aegypti and Ae. albopictus, public health agencies and can help
seasonal winter temperatures in the area during the week of the traveler’s return were not permissive for Aedes inform recommendations to prevent
† Zika virus infections.
activity. Maximum area temperatures during the week of the traveler’s return were <12°C (<54°F) and thus not
suitable for overwintering Aedes eggs to hatch and resulting larvae to survive. BG-Sentinel (Biogents AG, Regensberg,
Germany) and gravid mosquito traps placed around the residential areas of patients A and B in January yielded only
Culex but no Aedes mosquitoes.

Discussion ∠ Top

In addition to the present case report, at least five other cases of sexually transmitted Zika virus infection supported by laboratory evidence have now been
reported in the published literature; all were male-to-female transmissions involving vaginal sex. All of the male travelers had symptoms consistent with Zika virus
infection and could have transmitted infections to their sex partners a few days before or after as well as during the time symptoms appeared (3–5). In this case
report, patient B’s potential exposures occurred both before and just after initial appearance of symptoms in the traveler, which is the time when blood viremia
appears to be highest (i.e., as clinical signs and symptoms of infection emerge).§

Transmission of Zika virus to patient B by Ae. aegypti or albopictus was unlikely based on environmental conditions. Even if these mosquito species had been
present and active, the time from exposure to illness in patient B (i.e., 6–8 days) was shorter than the minimum estimated time required for Aedes to become
infectious had a mosquito ingested a Zika virus-infected blood meal from patient A (i.e., Ae. aegypti extrinsic incubation period is a minimum estimated duration of
10 days) (6,7), and for patient B once infected to have then developed illness (i.e., 3–12 days).

Studies investigating seminal shedding of infection-competent Zika virus, including its incidence, pattern (e.g., intermittent shedding or a steady decay), and
duration are ongoing. At the time of Patient B’s clinical presentation, there had been only one published report describing testing of semen from a man with Zika
virus infection (8); studies of semen from two additional men have since been reported (9,10). Zika virus has been detected by RT-PCR and isolated in culture from
the semen of two men at least 2 weeks after onset of illnesses (8,10) and possibly up to 10 weeks after illness in one of these cases (8). One report described Zika
virus detectable in semen by RT-PCR 62 days after illness onset; culture was not performed (9). In two men, Zika virus was no longer detectable in their blood by
RT-PCR when the semen specimens were analyzed (8,9). None of the three men provided follow-up semen specimens to determine when Zika virus was no longer
detectable. Notably, all men in the five case reports and the three semen studies, as well as patient A, experienced symptomatic illness. In the report of the sexual
transmission case that occurred in 2008 (1) and of the man with culturable Zika virus in semen in 2013 (8), symptoms also included hematospermia.

Identifying and characterizing cases of sexually transmitted Zika virus infection in areas experiencing intense autochthonous vector-borne Zika virus
transmission is challenging. Reports of sexual transmission identified in areas where autochthonous transmission is not occurring offer unique and important
opportunities to learn about this emerging mode of transmission and rapidly inform and refine interim prevention recommendations. Such cases highlight the
need for clinicians to remain vigilant for and continue reporting any suspected cases of Zika virus infection to their state or local health departments, including
suspected infections in symptomatic persons without travel history, but who report unprotected sexual contact with a person who has traveled to an area with
active Zika virus transmission.

Acknowledgments ∠ Top

Patients A and B; Division of Vector-Borne Disease Arboviral Laboratory, CDC, Ft. Collins, Colorado; Nicole Evert, Texas Department of State Health Services;
Scott Sawlis, Spencer Lockwood, Environmental Health Vector Control Division, Dallas County Health and Human Services, Texas; Joey Stringer, Daniel Serinaldi,
LRN Laboratory, Dallas County Health and Human Services, Texas.

∠ Top
Corresponding author: John T. Brooks, MD, zud4@cdc.gov (mailto:zud4@cdc.gov), 404-639-3894.
1Medical office of Steven M. Pounders, MD, Dallas, Texas; 2Acute Communicable Disease Epidemiology Division, Dallas County Health and Human Services,
∠ Top
Texas; 3Division of HIV/AIDS Prevention, National Center for HIV, Hepatitis, TB and STD Prevention, CDC; 4Division of Vector-Borne Diseases, National
Center for Emerging and Zoonotic Infectious Diseases, CDC, Ft. Collins, Colorado; 5Epidemic Intelligence Service, CDC.

References ∠ Top

1. Foy BD, Kobylinski KC, Chilson Foy JL, et al. Probable non-vector-borne transmission of Zika virus, Colorado, USA. Emerg Infect Dis 2011;17:880–2. CrossRef
(http://dx.doi.org/10.3201/eid1705.101939) PubMed (http://www.ncbi.nlm.nih.gov/pubmed/21529401)
2. Lanciotti RS, Kosoy OL, Laven JJ, et al. Genetic and serologic properties of Zika virus associated with an epidemic, Yap State, Micronesia, 2007. Emerg Infect
Dis 2008;14:1232–9. CrossRef (http://dx.doi.org/10.3201/eid1408.080287) PubMed (http://www.ncbi.nlm.nih.gov/pubmed/18680646)
3. Calisher CH, Karabatsos N, Dalrymple JM, et al. Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal
antisera. J Gen Virol 1989;70:37–43. CrossRef (http://dx.doi.org/10.1099/0022-1317-70-1-37) PubMed (http://www.ncbi.nlm.nih.gov/pubmed/2543738)
4. Venturi G, Zammarchi L, Fortuna C, et al. An autochthonous case of Zika due to possible sexual transmission, Florence, Italy, 2014. Euro Surveill
2016;21:30148. CrossRef (http://dx.doi.org/10.2807/1560-7917.ES.2016.21.8.30148) PubMed (http://www.ncbi.nlm.nih.gov/pubmed/26939607)

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5. Hills SL, Russell K, Hennessey M, et al. Transmission of Zika virus through sexual contact with travelers to areas of ongoing transmission—continental United
States, 2016. MMWR Morb Mortal Wkly Rep 2016;65:215–6. CrossRef (http://dx.doi.org/10.15585/mmwr.mm6508e2) PubMed
(http://www.ncbi.nlm.nih.gov/pubmed/26937739)
6. Boorman JP, Porterfield JS. A simple technique for infection of mosquitoes with viruses; transmission of Zika virus. Trans R Soc Trop Med Hyg 1956;50:238–
42. CrossRef (http://dx.doi.org/10.1016/0035-9203(56)90029-3) PubMed (http://www.ncbi.nlm.nih.gov/pubmed/13337908)
7. Hayes EB. Zika virus outside Africa. Emerg Infect Dis 2009;15:1347–50. CrossRef (http://dx.doi.org/10.3201/eid1509.090442) PubMed
(http://www.ncbi.nlm.nih.gov/pubmed/19788800)
8. Musso D, Roche C, Robin E, Nhan T, Teissier A, Cao-Lormeau VM. Potential sexual transmission of Zika virus. Emerg Infect Dis 2015;21:359–61. CrossRef
(http://dx.doi.org/10.3201/eid2102.141363) PubMed (http://www.ncbi.nlm.nih.gov/pubmed/25625872)
9. Atkinson B, Hearn P, Afrough B, et al. Detection of Zika virus in semen. Emerg Infect Dis 2016;22. CrossRef (http://dx.doi.org/10.3201/eid2205.160107)
10. Mansuy JM, Dutertre M, Mengelle C, et al. Zika virus: high infectious viral load in semen, a new sexually transmitted pathogen? Lancet Infect Dis 2016;16:405.
CrossRef (http://dx.doi.org/10.1016/S1473-3099(16)00138-9) PubMed (http://www.ncbi.nlm.nih.gov/pubmed/26949027)

∠ Top
* Pan American Health Organization and World Health Organization Regional Office of the Americas. Epidemiological alert. Neurological syndrome,
congenital malformations, and Zika virus infection. Implications for public health in the Americas — 1 December 2015.
†
National Weather Service Climate Prediction Center. Temperature data for Dallas-Ft. Worth, Texas. 2016.
http://www.cpc.ncep.noaa.gov/products/tanal/temp_analyses.php (http://www.cpc.ncep.noaa.gov/products/tanal/temp_analyses.php) .
§http://www.who.int/bulletin/online_first/16-171207.pdf (http://www.who.int/bulletin/online_first/16-171207.pdf) .

TABLE
TABLE. Reverse transcription polymerase chain reaction (RT-PCR) and serologic testing of serum from patients A and B — Texas, ∠ Top
January 2016

Days after symptom ZIKV RT- ZIKV DENV CHIK ZIKV DENV-1 DENV-2
Patient onset PCR IgM* IgM IgM PRNT† PRNT† PRNT†

Patient 14 Negative Positive Positive Negative >20,480 >20,480 5,120

A

Patient 4 Negative ND ND ND 160 <10 <10

B

Patient 7 Negative Positive Positive Negative 2,560 10 <10

B

Abbreviations: CHIKV = chikungunya virus; DENV-1 or 2 = dengue virus serotype type 1 or 2; IgM = immunoglobulin M; ND = not done; PRNT = plaque-
reduction neutralization test; ZIKV = Zika virus.
* IgM antibody capture-enzyme linked immunosorbent assay.
† Serum dilution-plaque reduction neutralization test, titers of neutralizing antibodies to ZIKV, DENV-1, and DENV-2.

∠ Top
Suggested citation for this article: Deckard DT, Chung WM, Brooks JT, et al. Male-to-Male Sexual Transmission of Zika Virus — Texas, January 2016.
MMWR Morb Mortal Wkly Rep 2016;65:372–374. DOI: http://dx.doi.org/10.15585/mmwr.mm6514a3 (http://dx.doi.org/10.15585/mmwr.mm6514a3) .

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