Standard Operating Procedure

for work with Hydrophobization Solution.

PI: Building(s):
PI Signature: Room Number(s):
Date: Designated Work Area:
Departmental Approval? Yes ☐ No ☒

1. Hazard Identification
a. Preparation and Use:
For preparing the Hydrophobization Solution, we need to use
Toluene:
Concentration: Toluene (99% Concentration). Purchased from Sigma-Aldrich.
Quantity: 99.8% of Final solution
Frequency: Always use in the preparation of the Hydrophobization solution
Location: Flamable Storage.

Octatrychlorosilane(OTS)
Concentration: OTS (99% Concentration). Purchased from Sigma-Aldrich.
Quantity: 0.2% of the final solution
Frequency: Always use in the preparation of the Hydrophobization solution
Location: Flamable Storage.

b. Potential Hazards and Risk:

Toluene:
OSHA Permissible Exposure Limit (PEL):

200 ppm (TWA); 300 ppm (acceptable ceiling conc.); 500 ppm (maximum conc.).

Toxicological Data:

Oral rat LD50: 636 mg/kg; skin rabbit LD50: 14100 uL/kg; inhalation rat LC50: 49 gm/m3/4H; Irritation
data: skin rabbit, 500 mg, Moderate; eye rabbit, 2 mg/24H, Severe. Investigated as a tumorigen, mutagen,
reproductive effector.

Reproductive Toxicity: Has shown some evidence of reproductive effects in laboratory animals.

Inhalation: Inhalation may cause irritation of the upper respiratory tract. Symptoms of
overexposure may include fatigue, confusion, headache, dizziness and drowsiness.
Peculiar skin sensations (e. g. pins and needles) or numbness may be produced. Very
high concentrations may cause unconsciousness and death.
 Ingestion: Swallowing may cause abdominal spasms and other symptoms that parallel over-
exposure from inhalation. Aspiration of material into the lungs can cause chemical
pneumonitis, which may be fatal.

Skin Contact: Causes irritation. May be absorbed through skin.

Eye Contact: Causes severe eye irritation with redness and pain.

Chronic Exposure: Reports of chronic poisoning describe anemia, decreased blood cell count and bone
marrow hypoplasia. Liver and kidney damage may occur. Repeated or prolonged
contact has a defatting action, causing drying, redness, dermatitis. Exposure to
toluene may affect the developing fetus.

Octatrycholorosilane:
If inhaled If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a
physician.

In case of skin contact Take off contaminated clothing and shoes immediately. Wash off with soap and
plenty of water. Consult a physician.

In case of eye contact Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician.
Continue rinsing eyes during transport to hospital.

If swallowed Do NOT induce vomiting. Never give anything by mouth to an unconscious person. Rinse
mouth with water. Consult a physician

2. Hazard Control
a. Selection and Purchasing:
Toluene:
Brown Glass Bottle 1Lt
Octatrychlorosilane
Browm Glass Bottle 100ml

b. Engineering Controls:
Always handle toluene and Octatrychlorosilane in a certified chemical fume hood, ducted biosafety cabinet,
or a glove box

c. Administrative and Work Practice Controls:

d. Personal Protective Equipment (PPE):
Toluene:

Skin Protection: Wear impervious protective clothing, including boots, neoprene or nitrile gloves, lab
coat, apron or coveralls, as appropriate, to prevent skin contact.

Eye Protection: Use chemical safety goggles and/or a full face shield where splashing is possible.
Maintain eye wash fountain and quick-drench facilities in work area.

Personal Respirators: If the exposure limit is exceeded and engineering controls are not feasible, a half-face
organic vapor respirator may be worn for up to ten times the exposure limit, or the maximum use
concentration specified by the appropriate regulatory agency or respirator supplier, whichever is lowest. A
full-face piece organic vapor respirator may be worn up to 50 times the exposure limit, or the maximum use
concentration specified by the appropriate regulatory agency or respirator supplier, whichever is lowest. For
emergencies or instances where the exposure levels are not known, use a full-face piece positive-pressure,
air-supplied respirator. WARNING: Air-purifying respirators do not protect workers in oxygen-deficient
atmospheres

Octatrychlorosilane:
Respiratory protection

Where risk assessment shows air-purifying respirators are appropriate use a full-face respirator with multi-
purpose combination (US) or type ABEK (EN 14387) respirator cartridges as a backup to engineering
controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use
respirators and components tested and approved under appropriate government standards such as NIOSH
(US) or CEN (EU).

Hand protection

Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without
touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after
use in accordance with applicable laws and good laboratory practices. Wash and dry hands.

Eye protection

Tightly fitting safety goggles. Faceshield (8-inch minimum). Use equipment for eye protection tested and
approved under appropriate government standards such as NIOSH (US) or EN 166(EU).
 Skin and body protection

Complete suit protecting against chemicals, Flame retardant protective clothing, The type of protective
equipment must be selected according to the concentration and amount of the dangerous substance at the
specific workplace.

Hygiene measures

Handle in accordance with good industrial hygiene and safety practice. Wash hands before breaks and at the
end of workday.

Specific engineering controls

Use mechanical exhaust or laboratory fumehood to avoid exposure.

e. Storage and Transportation:

Toluene
1.-All work with toluene is to be done in a "toluene" designated area in order to keep toluene contamination
to a minimum. Any persons in this area are required to wear personal protective equipment. Safety shower
and eye wash stations should be easily accessible where toluene is used.

2.- All laboratory equipment (such as beakers, pipettes, etc.) used in the "toluene" designated area are to be
labeled as "toluene contaminated" and are not to be removed from the area without first being
decontaminated.

3.-Protect against physical damage. Store in a cool, dry well-ventilated location, away from any area where
the fire hazard may be acute. Outside or detached storage is preferred with signage stating “Reproductive
Toxin” both on the storage and on the chemical container.

4.- Separate from incompatibles. Containers should be bonded and grounded for transfers to avoid static
sparks. Storage and use areas should be No Smoking areas. Use non-sparking type tools and equipment,
including explosion proof ventilation. Containers of this material may be hazardous when empty since they
retain product residues (vapors, liquid); observe all warnings and precautions listed for the product.

Octatrychlorosilane:
Precautions for safe handling
Avoid inhalation of vapour or mist. Keep away from sources of ignition - No smoking. Take measures to
prevent the build up of electrostatic charge.
Conditions for safe storage
Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be
carefully resealed and kept upright to prevent leakage. Never allow product to get in contact with water
during storage. Air sensitive.
3. Emergencies, Spill Procedures, and Exposures/Unintended Contact
First Aid Procedures

Inhalation: If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is
difficult, give oxygen. CALL A PHYSICIAN IMMEDIATELY.

Ingestion: Aspiration hazard. If swallowed, DO NOT INDUCE VOMITING. Give large quantities of
water. Never give anything by mouth to an unconscious person. Get medical attention immediately. If
vomiting occurs, keep head below hips to prevent aspiration into lungs.
Skin Contact: In case of contact, immediately flush skin with plenty of soap and water for at least
15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse.
Call a physician immediately.

Eye Contact: Immediately flush eyes with plenty of water for at least 15 minutes, lifting lower and
upper eyelids occasionally. Get medical attention immediately.

Spill and Accident Procedure

Chemical Spill Dial 911 and x59797

Spill – Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. Eliminate
sources of ignition if the chemical is flammable. If possible, confine the spill to a small area using a spill kit or
absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal
protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags,
label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower
for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15
minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S at x59797
immediately.
 Medical Emergency Dial 911 or x52111

Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911 (or 310-825-1491
from cell phone) or contact the Ronald Reagan UCLA Medical Center (emergency room) directly at x52111
(located at 757 Westwood Plaza, enter from Gayley Avenue). Note: All serious injuries must be reported to
EH&S at x59797 within 8 hours.

Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF), x56771, CHS room
67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the School of Dentistry on Tiverton Drive
and proceed to the “O” elevator to the 6th floor.)Hours: M - F, 7:30 a.m. to 4:30 p.m. At all other times report
to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All serious injuries must be
reported to EH&S at x59797 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash the affected
area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the
affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 231 from a
campus phone, enter 93333 when prompted and then enter your extension. Hours: M – F, 8:00 a.m. to 4:00
p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All
needle stick/puncture exposures must be reported to EH&S at x59797 within 8 hours.

4. Waste

1. Whatever cannot be saved for recovery or recycling should be handled as hazardous waste.
2. All solid toluene contaminated waste shall be disposed of into clear double bag waste specifically
designated for toluene waste. Examples of solid toluene waste material include gloves, pipette tips,
and paper towels.
3. Once the waste container is full, dispose of according to the hazardous waste guidelines.

5. Details of Process
1.- Clean the glass surface to be coated with the ultrasonic bath

2.- Prepare the OTS solution:

Make sure that all OTS have been transferred to the toluene, the concentration of OTS is kept at 0.2 wt.%.
e.g. 100ml of Toluene in a baker with 0.2ml of OTS and stirring softly.

3.- Insert the glass surface into the OTS solution. Make sure that the glass surfaces are dry. Incubated for 15
 to 20 min max.

4.- Transfer the glass surfaces to a baker containing only toluene for 1 hr.

5.- Let dry

6.- leave the dry glass surface in an oven set at 90 degrees Celsius for haft and hour or longer.

6. Training
All personnel are required to complete the online General Lab Safety. Furthermore, all personnel shall read and
fully adhere to this SOP when handling the chemical.

“I have read and understand this SOP. I agree to fully adhere to its requirements.”
Last First Student ID Signature Date