Store at -20°C

PERK (D11A8) Rabbit mAb

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orders@cellsignal.com
#5683

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Web n www.cellsignal.com
rev. 02/04/16

For Research Use Only. Not For Use In Diagnostic Procedures.

Entrez-Gene ID #9451
Swiss-Prot Acc. #Q9NZJ5

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150

Applications Species Cross-Reactivity* Molecular Wt. Isotype
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
W, IP, IHC-P H 140 kDa Rabbit IgG**
sodium azide. Store at –20°C. Do not aliquot the antibody.
Endogenous
*Species cross-reactivity is determined by western blot.
Background: PERK (protein kinase-like endoplasmic **Anti-rabbit secondary antibodies must be used to
reticulum kinase) is an eIF2α kinase and transmembrane detect this antibody.

7
18
CF

3
kDa

LN
29
M
protein resident in the endoplasmic reticulum (ER) mem-
200 Recommended Antibody Dilutions:
brane that couples ER stress signals to translation inhibition
140 PERK Western blotting 1:1000
(1-3). ER stress increases the activity of PERK, which then
100 Immunoprecipitation 1:50
phosphorylates eIF2α to promote reduced translation.
80 Immunohistochemistry (Paraffin) 1:2000†
PERK-deficient mice have defects in pancreatic β cells sev-
Unmasking buffer: Citrate
eral weeks after birth, suggesting a role for PERK-mediated 60
Antibody diluent: SignalStain® Antibody Diluent #8112
translational control in protecting secretory cells from ER 50
Detection reagent: SignalStain® Boost (HRP, Rabbit) #8114
stress (4). PERK activation during ER stress correlates with 40
†Optimal IHC dilutions determined using SignalStain® Boost IHC
autophosphorylation of its cytoplasmic kinase domain (1-3). 30 Detection Reagent.
Phosphorylation of PERK at Thr980 serves as a marker for
its activation status. For product specific protocols and a complete listing
20
of recommended companion products please see the
Specificity/Sensitivity: PERK (D11A8) Rabbit mAb rec-
product web page at www.cellsignal.com
ognizes endogenous levels of total PERK protein. Staining
10
of red blood cells has been observed. The specificity of this
staining is unknown.
Western blot analysis of extracts from various cell lines using
Source/Purification: Monoclonal antibody is produced PERK (D11A8) Rabbit mAb.
by immunizing animals with a synthetic peptide corre-
sponding to residues surrounding Leu156 of human PERK
protein.
Background References:
(1) Harding, H. et al. (1999) Nature 397, 271-274.
SignalStain, Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

(2) Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509.

(3) Harding, H. et al. (2000) Mol. Cell 5, 897-904.
(4) Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.

Immunohistochemical analysis of paraffin-embedded human

breast carcinoma using PERK (D11A8) Rabbit mAb.
© 2015 Cell Signaling Technology, Inc.

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,
0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Tween is a registered trademark of ICI Americas, Inc.
Applications Key: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide
Species Cross-Reactivity Key: H—human M—mouse R—rat Hm—hamster Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine
Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—horse All—all species expected Species enclosed in parentheses are predicted to react based on 100% homology.