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Brewer Spent Grain (BSG) is one of a solid waste generated from brewing process which contributes more

than 80% of total waste. Specifically, it contains approximately 50% fiber which consist of cellulose and
hemicellulose. Other components such as arabinoxylan, lignin, lipid, and ash were present in the BSG. BSG
mostly used to feed livestock due to high content of fiber and protein in the BSG that could be as nutrients
for livestock. The processes were initiated by pretreatment where 75 grams of wet BSG treated with H2SO4
and NaOH in 97oC in order to disrupt the polysaccharides and remove the lignin as well as improve sugar
formation in the BSG. Pretreatment process was finalized with pH neutralization by washing the samples
using demineralized water. Enzymatic hydrolysis is done to convert cellulose as one of the main
components of BSG into fermentable sugar (glucose) using cellulase enzyme and β-glucosidase within 48
hours and 72 hours. Glucose meter being used to specify how many grams glucose in 1 liter of solution. It
shows 2.94; n/a; 3.80; 3.94 g/L respectively. Glucose-water solution as the result of hydrolysis process was
fermented in a batch. Yeast of saccharomyces cerevisiae were added to ferment the glucose-water solution
into ethanol-water solution. The fermentation process last for 48 hours and 72 hours. The ethanol presence
after the fermentation separated by distillation under 90oC. The ethanol content determination done by
reflectometer which shows reflect index value (Nd) of ethanol-water solution 1.3385; n/a; 1.3370; 1.3430
respectively. The Nd values converted into ethanol content in the solution give 15%; n/a; 12.35%; 22.90%
v/v. Variable of pretreatment temperature, hydrolysis time, and fermentation time give different results
where glucose and ethanol concentration vary and specify certain yield. Therefore, the yield of ethanol is
0.705; n/a; 1.740; 3.226 grams ethanol over 100 grams of BSG.