1

M/s. Qiagen India Pvt. Ltd.,

Brand : ROTOR GENE Q MDX, Model : ROTOR GENE Q MDX 5PLEX HRM
[A] Technical Specifications required as per T.E. Spec. as per
Tech. Spec. provided as Tech. Spec. Remarks of Technical
Supplementary
per Tech. Bid Demonstrated Committee
Documents
Should be automated system for real time PCR and Post
1
PCR analysis- Critical
Should be an open system real time PCR assay system
2 with peltier / air based technology for heating and
cooling- Critical
Should be standardized for Taqman and Syber green
3
chemistry. - Critical
Should support the following applications
4
Absolute quantification- Critical
a)
Relative quantification- Critical
b)
Multiplex PCR- Critical
c)
Dissociation curve analysis- Critical
d)
Melt curve analysis- Critical
e)
Alleleic discrimination- Critical
f)
HRM – Critical
g)
Should allow normalization of reactions due to non PCR
related fluctuations by addition of any passive
5
reference calibrated dye or optional or should be
provided by the hardware itself.- Critical
Thermal Block / Rotor –
6
96 wells / 72 well rotor, should support 0.2ml / 0.1ml
a)
optical PCR tubes - Critical
Temperature range of 30C to 99C- Critical
7

2
 M/s. Qiagen India Pvt. Ltd.,
Brand : ROTOR GENE Q MDX, Model : ROTOR GENE Q MDX 5PLEX HRM
[A] Technical Specifications required as per T.E. Spec. as per
Tech. Spec. provided as Tech. Spec. Remarks of Technical
Supplementary
per Tech. Bid Demonstrated Committee
Documents
Should provide a RAMP rate of 2-5 deg. C per sec. -
8
Critical
Should be able to support hot start PCR cycle- Critical
9
System should support a reaction volume from 10 to
10
30l. - Critical
Optics – Excitation by tungsten halogen lamp or LED or
11 Xenon lamp and detection by CCD cameras
/photodiodes/PMT- Critical
Should have six fluorescence excitation and emission
12 filters and data should be collected for all filters for all
wells- Critical
Platform should provide following calibrated dyes at
installation-SYBR green, FAM, VIC, JOE, NED, TAMRA,
13
Cy-3, and Texas red, Cy-5. Should offer flexibility in
selection of dye for each filter. - Critical
Dedicated licensed full version software must be
14
included in the supply. - Critical
Platform should be IVD certified – Critical
15
Analysis Software and computer / laptop having
16 Microsoft system and relevant analysis software must
be provided with the system- Critical
Calibration or validation plates should be provided
17
wherever applicable- Critical
Power in put to be 220-240V, 50Hz- Critical
18
CE marked / FDA approved. – Critical
19
Suitable online UPS of two hours should be provided-
20
Critical
21 Must provide list of end users and should have at least
five installations in reputed institutions or labs in India -

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 M/s. Qiagen India Pvt. Ltd.,
Brand : ROTOR GENE Q MDX, Model : ROTOR GENE Q MDX 5PLEX HRM
[A] Technical Specifications required as per T.E. Spec. as per
Tech. Spec. provided as Tech. Spec. Remarks of Technical
Supplementary
per Tech. Bid Demonstrated Committee
Documents
Critical
Operation manual with user demonstration video CD.

Acceptable

Not acceptable

Remark

Name & Signature of Technical Experts: