GENE A SER DELETADO: AN7913

>AN7913
CTTGTTTTATGAGATTATGTGTTATGGAGTGAGTATGAAGAGACTGAGTAGAGATTGCTGTTTGAAGATGGACAGAGACGAGACTG
GACAATGGGCATGGAATGGGAAACCATCAGGGCAATGATGGTGAAACGGTGGCAATATATACATCTTGAGGCTGTCAAGGATTTTT
AACAATCAACCAAATGGCAGGTGCGATTCTGTAGCAGTACAGAAGAGATGAGGACGAGGGGACAAGGTGACGAGGGTGAGTCCAGA
GTATCCCAGTACATAGAAGAGAACGGCACCTGCCGTTCTCTCTCGCTTATTGCAAGCCGCTTGCAAGAATAAGAACAGCAGGTGTG
GTTCTACTCTTAGTCAGCTATGCTTATAAACAGGACGGTACGGTACGGTACTGTATGGTACTGTCGTTGCCTGTTCGGCTTGGCAT
TCTTCTCATTTCACTCAATCCTGCTCGAGTCGTGTAAAGCCTCTCGTCTGTGAGCAACCTCCCTCCCTCCGACCTCAATCGCACTC
CTTCCTCTGGAGCTCATCATGTCTCTTGCACGACAAGATGCCCCCAGTGAGATTTGGACCCCGGAAGATCCTCGAGTACGACGGTC
GCTACGGGGTTCTGATCTGCCACGAGTGCCGGTATGCGATCCAGAAGAGCGCGCTGCAGAGCCACCTGCTGCGCCATAAGATCTAC
CGTGCCGACCGCCAGCAGCTGGTCGCTATGATTAATGAGCTCGATCTTCTCGAGCCTGACGATGTGTTGCTGCCGCCTCCGGAGTC
ACCCCCGATCGACGGGCTGCCGGTCATCGCGGGGTACCGCTGTACAGCCCCGGGTTGCGCTAATCTCTGTGCGAGTCTGAAGCGCA
TGAAGGGCCATTGGAGAGAGAGCCATGGTATTGCGGATGCGTCACTCGCGCGTCCAGCCAAGCTACAGACTTTCTTTCGGGGGACT
AAGATCCGCTACTTTGAGGTGACTCCCACGACAGAGGATGAGGACGATGAGGAAAACGAGAGCGAGAATGACGAGGAAGAAGGGGA
TGTCGATTTGGAAGAGCAGGAAGACGACAACGGTGGTCGGCAGTCAACGACGGTCACAACTTCTCCCGGCCCTTCCGCTCCCTCTG
TCAACGTGGACCTTGAAACCCTGTCCTACTTTCACCATTTCATGTCGGCAACGAGCCTGACATTACCATGTCCGCAGGATATGCAG
TCGGGGGCTCAATACTGGAAAGAAAAGGCAGTCCCTCAAGCGCTCCAGCAGAGATGGTTGATGTGCGGTCTGCTGGCGCTCGCTGC
CTGTCACTTGGCCGCATTCCCGGACAATGCGGCGGCTGGCCAACAGCACCGCAAACGAGCGGCTGAATTCTCCCTCGAGTTTCGAA
CGGGATGGAGAGAACTGGCCGACACATCTGGTGAGGGTCTGCGAGAGGTAGCGACCGAGATTGAGTGTTTGCTACGTTGTGCACAT
TGGGCATTGGCTGAATCCCCCTGCGATCAACGCATCATGCCAGAGCCGGGCGTACCAGAGCACCTCCAGTCCATCATCAGCACCAT
TCAGAGTACTGTTCCCGCGGCCGCACCACACGAAGCTGAAACGTCGGCCTACGCGACACGAATTCTCAGATGGAACACCTCAGAGG
CCGGAAACAGCGTCCTTGCGGAGATCCGGAACCGTCTTCATGACCTACCAGCTCGCATGGCCGATACCTTTGGGAGACCGGAGAAT
ATTCAGGACGTTCTTGTCCTCCTGTCAGCCCTCGCAGCTATGGGTGAGTGCTGTGACACGAGCTTTGCGTCGGAGGAAGTTGGACC
AGCGTGGTGGGGGATGGCGACCTGGTGGACGCGAGTGCCGCTTCGTTTCAAGGAGCTGGTGGCACGCCACTATCCAGCATCTCTCG
TGGTGGTCGCCCATTGGGCAGCACTGATGGTCAATCGAACCGAGCGCTGCGGATGCTGGCTAGTCAAGGGGTTGGCCATGACGATC
TTACTACGGATCGCTGAGCGACTGCCGGAGGACGATGACGGAAATGTGCAGCGGCTTGTTGCGCTTACTATTGCGGCCTAGACTAA
TGATTACCAGACACATGCGGTTTCGCTACAGCCCTTTGCCAAAGCACGGCCTGAAGATTTGATAATCTGGCTAGCTGGCCGGGGCG
CTTTCTGCTAGGATATCCTTGCATCTGATAGAAGCATACATACTAGCCTGCAGTGATATCATACATAACCTAAGCTGGCTGTGCTC
AGTCAAGACAAGAAGAATTAAATTTAGATATGCATATCTTCTAATCAAAGCTTCTATAATTATACAATCAGGCAATATCGCGAGAC
CGTGCGATTCCGACCGGGCACCGACTAGGCACTGACTACGTTTTCTCCTCGAACAAACTGCATGCAGCAGCCGCATACTCGGGGTC
CAAGCCGGTACCAGCCAAGAGAGCCACTCATCCGTGGAGTGTCCCCATTCGCCAATTACGTCTCACGA

PROTOSPACER 1

PS1:ACAGAGACGAGACTGGACAA

PRIMER 1 (REV): AGTCTCGTCUCTGTTGCATCATCCGTGAATCGAAC

PRIMER 2 (FWD): AGACGAGACUGGACAAGTTTTAGAGCTAGAAATAGCAAG

PROTOSPACER 2 - ANTISENSE

PS2: GTTATGTATGATATCACTGC

PRIMER 3 (REV): ATCATACAUAACTGCATCATCCGTGAATCGAAC

PRIMER 4 (FWD): ATGTATGAUATCACTGCGTTTTAGAGCTAGAAATAGCAAG

OLIGO
AGACTGAGTAGAGATTGCTGTTTGAAGATGGACAGAGACGAGACTGATATCATACATAACCTAA
GCTGGCTGTGCTCAGTCAAGACAAGA

Montagem final
CSN438+PRIMER 1; PRIMER 2+ PRIMER 3; PRIMER 4 +CSN790GENE A SER
DELETADO: AN8772
>AN8772|zipB
GTCTCTGAGCCTTCAGTCTGGGATCTCTATGTGACTCATACTTACGAAACAGTCAGTCTCTGGGATACAAAATAGAACATCTAACC
GGGAATTACAAGCCTCTTAAGCACAGTCTAGGGAAGTCTGAGGAATAAAAATAGCACAGCCACTGACAAGGGCTAGACTCTGAAGG
GCAATTCGACGATTACTCAGTGCTCACCTCATCTTGTCTCACTGCGGCTGAGCTTCCGCCCAGAAGGAGAAACTGTGCCCAACGTG
CACGGCTGAGAACTGCTCTCCTCCCAGATATTTTCTTTCTTTTCTTTCTAGCACGAGAATGGATAAGCTCAAATCATTTACAAATA
GGTGGTCGACAAAAGGTATTATCTATCCGTTTCCATCTCATTCCCCTAACCAAACCCACCCTTCCCGGTAAAAGGACTAATAGGCT
ACAGAGAACGCCAGTGAGCCCACTGATCCAGGTCTAAGACGAAAAGAACAGGTCCGACGTGCACAGCAGTACGCTCTATTCTCATT
TCGGTCTTCCTTGGTGTAGCTAAACACAAGCAGAACCTACCGGCTCCGCAAGGAAAGCTACATCAAGTCTCTTGAGCGCGAGATCC
TGCACCTTCGCACTGCTAAGTCCGATTTAACAGGGGAAACCAGAAAGCTTAGGGCAGAGGTACGGCGGTTGCGCCAAGTGATTGAG
CAGCATGGTATAGTCCTTCCGTTTACGCCGCCATCTCTTGATTCCGACATCACATCCCCAAGACAGGAGAATAGTCCGCAGGGAGC
AATGGCGACGCTCTGCATAGATAGAGACCATCTGAACAATAAACGTCTGGCTGTCTTTGACGACGCTCTGACTTACGGGACGAGCC
CTGGGTCGGTTCAAACTGCGACTGAAGGTGTTCACCTCCCGGACGGTTCCTTTACGGGCCAGTCGGAAGTTGTAGCAGCCATGAAT
TTCATCCTCAGGTATGTCTGGTCTCCTCTCCATCAAATATAGTCCAGCAAGTAGTGGTTGAGAGTTCAGCCTCGAGGCGCCCTGTC
TAGACCACGTCCGCGCCGCACTAGGCGCCCCCTCTGCGCAAAGCGAAATGGGCCACGGCCATGCCCTAACCCTAACTGCTTCTGTC
TTTCGCCTTTACAGCGACACCTCACTCTTACCAGAGGAGGATGAGCTCCTTCATGTGTCGAAGCAGACATTAAGCCGACTGCTAGA
GCTTTCTGTGCAGGTTACCACGGCCGAGGAGCTCACGCCGACCCAGGTGTGGGCGTTCCTTCACCAAAGCCCTGCGTTTGCAGACC
CGAAGACACGACAAGAGAAGATCATGAATGTTGCGCAGGAGTTGGCAAGGTATATGAAGTGTCATGGGTAGGATTCCATCTGTGAT
AATATGCACCTTTCTTACTAATGGGACCAGGTATGGAGCGGTTATTCCAAGAGATGTGGTTACGAGGGTTGTCTCTCAGGTTATCC
CTCCACAAGGGTCATGGCAGGTGTGACTTGGGCAAAGCCATCTACCTTTTCGAGGCCTTTCTAGACTCTTATTACATGGTCTCATG
CCGGGAGAAAGGTTCTAGAATTGCAAAATCATCCGACAGCAGTACCGCAAACTGCTGCTGAGCCTGGCTTCTGCCCTTTCAGATCT
CCACGTATAACTCAAAAACATTGATAACTTTAGCCTTGCAAATACCATGCTAGCAGATGCATTCCCTTCTGACAGGCAGTTTGTGC
AATGAAAGGTTATTTATATGGCGGCTGATATCATCTACAAAAGTAGCCAGTTTTAGCAATTGTGATAGTAACATCTAGTTATAGAG
AAGAGAAAACTGTTGGCAAACCAACATGTACCTGTCGTTAGCTTTGGCTTCAGCATATATGATTACTTTTGCTGGCTCTCGAAGGA
TACCAAATACCGGAACTGCTCACGGGAGATTATC

PROTOSPACER 1

PS1: CACCTCATCTTGTCTCACTG

PRIMER 1 (REV): AGACAAGAUGAGGTGTGCATCATCCGTGAATCGAAC

PRIMER 2 (FWD): ATCTTGTCUCACTGGTTTTAGAGCTAGAAATAGCAAG

PROTOSPACER 2 - ANTISENSE

PS2: AGTCTAGAAAGGCCTCGAAA

PRIMER 3 (REV): AGGCCTTTCUAGACTTGCATCATCCGTGAATCGAAC

PRIMER 4 (FWD): AGAAAGGCCUCGAAAGTTTTAGAGCTAGAAATAGCAAG

OLIGO
TGAAGGGCAATTCGACGATTACTCAGTGCTCACCTCATCTTGTCTAGGCCTTTCTAGACTCTTA
TTACATGGTCTCATGCCGGGAGAAAG

Montagem
CSN438+PRIMER 1; PRIMER 2+ PRIMER 3; PRIMER 4 +CSN790