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MSM Trace Element (TE) solution

Article · March 2015

DOI: 10.1038/protex.2015.032

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Stephan Christel Mark Dopson

Linnaeus University Linnaeus University
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 Dopson, Systems Biology of Microorganisms

MSM Trace Element Solution (TE)

Description: Trace element solution (TE) for Mineral Salts Medium (MSM, see separate protocol).
To be added when medium is not supplied with mineral. Add 1 ml of 100x Stock TE
solution into 99 ml of MSM.

Materials: Trace Element solution:

- Ferric chloride hexahydrate, FeCl3 x6H2O
- Copper sulfate pentahydrate, CuSO4 x5H2O
- Boric acid, HBO3
- Manganese sulfate hydrate, MnSO4 xH2O
- Sodium molybdate dihydrate, Na2MoO4 x2H2O
- Cobalt chloride hexahydrate, CoCl2 x6H2O
- Zink sulfate heptahydrate, ZnSO4 x7H2O
- Sodium selenate, Na2SeO4

General:
- Sulfuric acid
- Sodium hydroxide

Procedure: For 100x Stock TE solution: - 1100 mg/l FeCl3 x6H2O

- 50 mg/l CuSO4 x5H2O
- 200 mg/l HBO3
- 200 mg/l MnSO4 xH2O
- 80 mg/l Na2MoO4 x2H2O
- 60 mg/l CoCl2 x6H2O
- 90 mg/l ZnSO4 x7H2O
- 10 mg/l Na2SeO4

- Add the constituents into a final volume of 1 liter of water (pH pre-adjusted to
around 2.5), one at a time, dissolving before adding the next.
- Adjust the pH to 1.5 with sulfuric acid (and/or sodium hydroxide)
-sterile filter

Troubleshooting: -TE solution should be clear and almost colorless. Strong coloration
indicates too high pH.

-When encountering difficulties during dissolution of a reagent, try

adding some more sulfuric acid.

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