Naga City Science High School

Barangay Balatas, Naga City

JUNIOR HIGH SCHOOL DEPARTMENT

FOURTH QUARTERLY EXAMINATION

BIOTECHNOLOGY
S/Y 2017 – 2018

Name: ______________________________________Year and Section: ____________ Score: ___________

 Do Good  8. How many codons are needed to specify three

amino acids?
Read each item carefully. Write the letter of the a. 3 c. 9
correct answer on your answer sheet. Erasures b. 6 d. 12
are not allowed. 5. Three of the bases found in nucleic acids are
pyrimidines and two are purines. Which is
1. Which is attached to the transfer RNA correct groupings based on the ring structures?
(tRNA)? Pyrimidines Purines
a. DNA c. ribosome a. adenine and thymine cytosine and guanine
b. amino acid d. nucleic acid b. adenine and cytosine thymine and guanine
2. Which is not part of protein synthesis? c. uracil and thymine adenine and guanine
a. replication c. translation d. cytosine and uracil thymine and cytosine
b. transcription d. mutation 10. What is the correct order of events during the
3. Which base is not found in RNA? synthesis of specific protein?
a. adenine c. cytosine
b. thymine d. uracil a. Messenger RNA attaches to a ribosome.
4. During the process of transcription, which is b. DNA serves as a template for RNA
produced? production.
a. H2O c. ATP c. Transfer RNA bonds to a specific codon.
b. mRNA d. DNA d. Amino acids are bonded together.
5. If the DNA template reads “ATA”, which e. RNA moves from the nucleus to the
would be the corresponding sequence on the cytoplasm.
mRNA?
a. UAU c. TUT a. B E A C D c. B C E D A
b. ATA d. UCU b. D A E C B d. C B A E D
6. What role does messenger RNA play in the 11. What is the complementary messenger-RNA
synthesis of proteins? sequence for the DNA sequence shown below?
a. it catalyses the process
b. it provides the genetic blueprint for the protein
c. it translates the genetic code to a specific a. C-A-A-G-G-U c. G-U-U-C-C-A
amino acid b. G-T-T-C-C-A d. C-A-A-G-G-T
d. it modifies messenger RNA molecules prior to Use the diagram below for Questions 12-14
protein synthesis
7. Which is an example of the degeneracy of the
genetic code?
a. a given amino acid has more than one codon
b. each codon specifies more than one amino 12. Which processes occur in the nucleus?
acid a. 1 and 2 c. 3 and 4
c. the first two bases specify the amino acid b. 2 and 3 d. 4 and 5
d. the genetic code is not degenerate

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13. Process 2 is known as 20. What is the role of tRNA during translation?
a. replication c. transcription a. bond to open the DNA strand to carry the
b. mutation d. translation code for protein synthesis out of the nucleus
14. What is the product of process 3? b. carry ribosomes to the site of protein
a. a strand of DNA synthesis
b. two complementary strands of DNA c. break aparty mRNA and send it back to the
c. a strand of RNA nucleus so that it can be reused
d. a chain of amino acids d. Carry amino acids to the mRNA for correct
Use the diagram below for Questions 15-17 placement into the protein chain
21. What cellular process is shown in the diagram
below?

15. Structure X was made in the _________. a. Replication c. Translation

a. nucleus c. lysosome b. Transcription d. Mutation
b. cytoplasm d. vacuole 22. What does Chargraff's Rule mean?
16. The process represented in the diagram is most a. Whatever Chargraff's says is correct
closely associated with the cell organelle known b. Guanine always binds with Cytosine and
as the ____________ . Adenine always binds with Thymine
a. nucleolus c. chloroplast c. Guanine always binds with Adenine and
b. ribosome d. mitochondrion Cytosine always binds with Thymine
17. Which amino acid would be transferred to the d. Guanine always binds with Thymine and
position of codon CAC? Adenine always binds with Cytosine
a. leucine c. valine 23. Why does DNA replication is said to be
b. glycine d. histdine semiconservative?
18. What makes up the nucleotides of DNA? a. both RNA and DNA synthesis are involved in
a. Sugar, Phosphate and Nitrogenous base the process
b. Ribose Sugar, Phosphate and Nitrogenous b. part of the telomere is lost during each round
base of replication
c. Deoxyribose Sugar, Phosphorus and c. a new double helix contains one old and one
Nitrogenous base new strand
d. Deoxyribose Sugar, Phosphate and d. each new strand is complementary, not
Nitrogenous base identical, to its template.
19. Which base pairs are found in DNA?
a. A–C and T–G c. A–G and C–T
b. A–T and C–G d. A-U and C-G

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24. What happens after the DNA polymerase laying 30. If a change occurs in a base sequence of a
down a new DNA strand meets up with the DNA, it might cause a different ___ to be
RNA primer of a preceding Okazaki fragment? substituted in a protein.
a. The other strand is then replicated in the 3 to a. ribosome c. nucleic acid
5’ direction b. amino acid d. enzyme
b. The DNA polymerase reverses direction and 31. Who is the first scientist showed that genetic
performs error checking material is a heat stable chemical?
c. DNA ligase couples the two fragments a. Franklin Griffith c. Friedrich Miescher
together b. Erwin Chargaff d. Oswald Avery
d. The RNA primer is removed and is replaced 32. Who is the scientist that identified the
by DNA. transforming agent in Griffith's famous
25. Why is an RNA primer necessary for DNA experiment (1928)?
replication? a. Erwin Chargaff c. Friedrich Miescher
a. The RNA primer is necessary for the activity b. Oswald Avery d. Peter Mitchell
of DNA ligase 33. The polarity in a DNA strand is indicated by
b. The RNA primer creates the 5’ and 3’ ends of referring to one end as the 3′ end and the other
the strand as the 5′ end. Which structure is on the 3′ end?
c. DNA polymerase can only add nucleotides to a. Hydroxyl group c. Nitrogenous base
RNA molecules. b. Phosphate group d. Amino acid
d. DNA polymerase can only add nucleotides to 34. Which bonds are broken during DNA
an existing strand. replication?
26. Which is not part of a molecule of DNA? a. hydrogen bonds between bases
a. deoxyribose c. phosphate b. phosphodiester bonds
b. nitrogen base d. ribose c. covalent bonds between bases
27. During DNA replication, a complementary d. ionic bonds between bases and phosphate
strand of DNA is made for each original DNA groups
strand. If a portion of the original strand is 35. Which technique was used to determine the
CCTAGCT, what will the new strand be? double-helical structure of DNA?
a. TTGCATG c. AAGTATC a. Electrophoresis
b. CCTAGCT d. GGATCGA b. Chromatography
28. The original strand of DNA is c. Centrifugation
ACGTCTCGA; the mutated strand d. X-ray crystallography
ACGTTCGA. What type of mutation is 36. Which is the pyrimidine bases found in DNA?
this? a. uracil and thymine
a. nucleotide base sustitution b. thymine and cytosine
b. nucleotide base deletion c. adenine and thymine
c. nucleotide base insertion d. cytosine and uracil
d. nonsense mutation 37. How Huntingting's chorea characterized?
29. The original strand of DNA is a. incongruent muscle movement
ACGTCTCGA, the mutated strand b. inability to speak
ACGTCTACGA. What type of mutation is c. disordered muscle movement and mental
this? deterioration
a. nucleotide base deletion d. weak eye sight and hearing power
b. nonsense mutation
c. nucleotide base sustitution
d. nucleotide base insertion

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38. Which of the following is a characteristic of 46. What is an effect of Cystic Fibrosis?
individual with Turner's syndrome? a. Blackened gums
a. normal female b. Paralysis
b. normal male c. Abnormally thick mucus
c. a male with rudimentary testes and d. Loss of eyesight
undeveloped penis 47. What type of gene mutation results from the
d. a female with rudimentary ovaries and insertion or deletion of nucleotides and alters
underdeveloped breasts the entire amino acid sequence from that point
39. Queen Victoria was carrier of hemophilia, a sex forward?
linked disease. What is true? a. Frameshift c. Nonsense
a. hemophilia, would have occurred more in her b. Missense d. Silent
male than female descendants 48. If gene ABC altered with the new gene BBC
b. queen's father must have had hemophilia What mutation occurred?
c. all of her sons would have had haemophilia a. deletion c. insertion
d. all of her sons would have had haemophilia b. substitution d. transversion
40. How many total chromosomes are shown in a 49. If gene ABCDEF mutated with the new gene
normal human karyotype? ACEFCD. What mutation occurred?
a. 2 c. 44 a. deletion c. inversion
b. 46 d. 23 b. substitution d. insertion
41. What is sex chromosomes of an individual with 50. The original strand ATC CAT mutated with the
Klinefelter syndrome? new gene ATC GCAT. What mutation
a. XXY c. XXX occurred?
b. XY d. XX a. deletion c. silent
42. The results of the karyotype indicate that an b. insertion d. transverse
individual has 3 chromosome #21. Which
genetic disorders do they have?
a. Edwards Syndrome
b. Patau's Syndrome
c. Down's Syndrome
d. Klinefelter's Syndrome
43. Which disorder results when blood cells are
misshapen, causing in a decrease of oxygen
and produced by a defective allele for a
polypeptide in hemoglobin?
a. Duschenne's muscular dystrophy
b. Turner's Syndrome
c. sickle cell anemia
d. Down Syndrome
44. What do you called the change in a gene due to
damage or being copied incorrectly?
a. mutation c. segregation
b. evolution d. meiosis
45. Which mutations results from one nucleotide
being substituted for another?
a. Deletion c. Insertion
b. Frameshift d. Point

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