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Morphology
Aeromonas & vibrio
- Facultative anaerob
- Rod, curve
- Sucrose, mannose-fermenting
- Non arabinose-fermenting
- Positive oxidase
- Polar flagella (most)
- Bisa hidup di 14-40oC (WIDE RANGE)
- Bisa hidup dengan banyak NaCl
- pH 6,5 – 9,0; susceptible to acid
Classification
1. V. cholerae:
Biotype:
2. V. parahaemolyticus
3. V. vulnificus
V. vulnificus & non O1 have polysaccharide capsule help dissemination
Virulence factors
LPS
Pathogenicity
TCP (by VPI 1) place of attachment of lysogenic bacteriophage Vibrio engulfed by
bacteriophage (CTX-omega) ace gene (acessory cholerae enterotoxin gene),zot gene (zonula
occludens toxin gene), cep (chemotaxis protein genes) [in O1 and O139]
Cholerae have Complex A-B toxin (like E.coli). Cara kerja toxin: mengubah ATP menjadi CAMP
menyebabkan waterry diarrhea (1,5 ltr). TCP membuat Vibrio dapat menempel di mucosse cell
layer. TCP dikode oleh TCP gene. ACE menyebabkan sekresi fluid, ZOT menyebabkan lusion of
tap junction. Vibrio parahaemolyticus memiliki adhesin and thermostabile direct hemolisin
(Kanagawa hemolysin)>> cause hemolisis. Kanagawa also increasing calcium intraceluler
sehingga meningkatkan sekresi cl.
Vibrio vulnificus mempunyai fx virulens: menghancurkan lysin dan memproduksi alkaline bile
product ketika masuk ke lambung. Dapat menghindari imun respons dengan memicu apotoksik
makrofag dan polisakarida (antifagosit). Punyai surface protein yang akan menempel di host
cells dan melepaskan cytolotic toxin.
Estuarine & marine enviroment >> water, chitinous selfish (oyster, calms, mussels).
V. cholera O139 in Bengal(the only serogroup outside O1 yang bikin pandemic) will produce
same toxin with O1.
Cholera transmitted by water & food not person to person 10 8 (hypochlorhydria 103-5). Must
cooking over 40*C.
V. parahaemolyticus >> incubation period 5-72 jam (rata-rata 24 jam), explosive watery
diarrhea
V. vulnificus >> 90% mortality. Caused septicemia after shellfish ingestion, wound infection with
contaminated sea water. Gejala: Fever, chills, vomit, diarrhea, abdominal cramp. Characteristic
of wound: swelling, erythema, pain, vesica >> tubula >> necrosis
Diagnosis:
Micoscopic examination : Small size (0.5-1.5-3 mikrometer), curve, find in stool (onset
cholerae), gram stained
Cultue: media carry-blair and in refrigerator (can’t in buffered-glycerol saline), Mac Conkey,
TCBS agar, enrichment broth that contain alkaline pepton broth (ph 8,6)
Treatment:
Rehydration
AEROMONAS
Aeromonas hydrophila, Aeromonas caviae, Aeromonas veronii biovar sobria
Cause diarrhea (dysentric diarrhea, blood, and leukocytes), wound infection, and opportunistic
sistemic in immunocompromised patient.
Fx virulence: endotoxin, hemolysin, heat labile, and heat stabile enterotoxin (cara kerja tidak
diketahui)