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ON
SOIL CHEMICAL ANALYSIS
FACULTY OF AGRICULTURE
2. All acids are highly corrosive and alkalies are highly caustic.
Therefore, use them carefully and do not spill them over your clothes,
body, the table or floor.
3. Handle glassware with care. Broken glass may cause injuries on the
parts of your body. Handle it properly and avoid breakage.
5. Instruments are very costly and their repair is very time consuming.
Therefore, handle them carefully.
2. First aid
3. Burns
a. Cloth burns:
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disinfectant after the washing treatment. Allow the skin dry and cover
the burnt skin with burnol.
Wash the skin thoroughly with rectified spirit, then with soap and
warm water.
4. Cuts
Allow minor cuts to bleed for a few seconds. If the cut is due to glassware
breakage then remove the glass particles. Apply a disinfectant and
bandage. In serious cuts, consult the doctor at once after washing the
part with disinfectant.
5. Eye accident
In all case consult the doctor at once. Meanwhile, first aid should be
done.
Proceed as for acid in the eyes, but wash with 1% boric acid
solution instead of bicarbonate.
6. Poisons
a. Acids
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Drink a lot of water followed by vinegar, lemon or orange juice,
solution of citric or lactic acid.
c. Other poisons:
d. Harmful gases:
The victim should at once be removed to the open air and the
clothing of the neck should be loosened. Inhale NH3 vapours to
counteract cl2 or Br2 fumes, if inhaled in small amounts. Gargles
with NaHCO3 solution are also helpful.
7. Fires
Turn out all the gas burners and switch off all the electric hot plates in
the vicinity. Remove the things which may burn. Dry sand may be
employed if fire extinguisher is not readily available.
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SOIL TESTING
5. Sampling should not be done from an area about 2-3 meters along
sides of a large field.
Depth of sampling
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2. For deep rooted crops or longer duration crops like sugarcane, or
under dry farming conditions, samples from different depths
depending on individual requirements should be obtained
4. For saline –alkali soils, salt crust if visible on the soil surface, or
suspected should be sampled separately and record the depth of
sampling. Generally, the sample may be drawn up to 15 cm depth
from surface for testing salinity and alkalinity/ acidity.
3. Mix the soil collected from various spots covering the entire area
thoroughly by hand on a clean piece of cloth or polythene sheet.
Sampling tools
Samples can be drawn with the help of (i) tube auger (ii) screw
auger (iii) post hole auger (iv) spade and (v) Khurpi
For sampling of soft and moist soil, tube auger, spade or khurpi is
satisfactory. A screw type auger is more convenient on hard or dry
soil while the post hole auger is useful for sampling excessively wet
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area like rice fields. If a spade or khurpi is used a “V” shaped cut
may be first made upto the plough layer and a uniformly 2 cm thick
slice is taken out from one clean side. Tube auger attached to a long
extension rod is convenient for sampling from lower depths.
3. Do not use bags or boxes previously used for storing fertilizers, salt
or any other chemical.
Labeling of samples
Information sheet
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Sl. Identification depth No. of Area Upland Irrigated Previous When manure
No mark of spots of the or (Y/N) crop fertilizer
sampling sampled field lowland raised applied last
1.
2.
Tick mark (√) the additional test required Recommendation sought for
2. Crush the soil clods lightly and grind with the help of wooden
pestle and mortar.
6. For certain type of analysis (e.g. organic carbon) grind the soil
further so as to pass it through 0.2 to 0.5 mm sieves.
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SOIL TESTING PROCEDURES
Soil pH
Reagent: Standard buffer solution: prepare buffer solutions of pH 4.0, 7.0 and
9.2 by dissolving one commercially available buffer tablets of above pH values
in 100 ml of freshly prepared distilled water.
Procedure
1. Weigh 10 gm of soil sample in 50 ml beaker
2. Add 25 ml of distilled water and stir well by a glass rod and keep for 30
minutes
3. Calibrate the pH meter with standard buffer solution
4. Again stir, just before immersing the electrodes and take pH reading.
Electrical conductivity
Instrument: Electrical conductivity meter
Procedure:
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Organic Carbon : ( Walkley and Black, 1934)
Glassware required: Conical flask (500ml), volumetric flask (1000ml), Beaker
(500 &100 ml), measuring cylinder (25 ml and 250 ml),
pipette (10 ml) and burette (50ml).
Reagents:
Procedure
Calculations:
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Available Nitrogen: Alkaline permanganate method (Subbiah and Asiza,
1956)
Reagents required:
Procedure:
1. Place 20 gm of soil in a distillation flask
2. Add 20 ml distilled water and 100 ml of 0.32% potassium
permanganate solution. Add little paraffin wax to avoid contact with
upper part of the neck of the flask.
3. Pipette 20 ml of 2% boric acid solution in 250 ml conical flask and
add few drops of mixed indicator to it. Dip the end of the delivery tube
into it.
4. Pour 100ml of 2.5% sodium hydroxide into the distillation flask and
cork it immediately.
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5. Distill the ammonia gas from distillation flask which will be collected
into the boric acid mix indicator solution. Collect approximately 50 ml
of distillate.
6. Titrate the collected solution against 0.02 N sulphuric acid till the
green colour disappears.
Calculation:
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Available Phosphorus (Bray and Kurtz, 1945)
Reagents required:
1. Bray,s P1 solution: 0.03 N NH4F + 0.025N HCl (Dissolve 1.11 gm of
ammonium fluoride of AR grade in 1 litre volumetric flask and to it
add 2.16 ml of concentrated hydrochloric acid and make up the
volume one litre)
2. Reagent A:
i. Dissolve 12gm of ammonium molybdate in about 250 ml of warm
distilled water.
ii. In a separate beaker dissolve 0.291 gm of antimony potassium
tartarate in 100 ml of warm distilled water.
iii. After cooling both the solution mix both the solution.
iv. 5 N H2SO4: Prepare one litrre of 5N sulphuric acid by diluting 140
ml of conc. H2SO4 to one litre.
v. After cooling mix ammonium molybdate, antimony potassium
tartarate and 5 N H2SO4 in 2 litre volumetric flask and make the
volume 2 litre.
Procedure:
1. Weigh 2.5 gm of soil in 100 ml conical flask
2. Add 25 ml of BrayP1 solution and shake for 5 minutes on a
mechanical shaker
3. Filter the solution in reagent bottle.
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4. Pipette out suitable amount of aliquot (2-5ml) in 25/50 ml volumetric
flask
5. Adjust the pH with the help of p-nitrophenol, 2% ammonia solution
and 5N sulphuric acid
6. Add 2 ml of ascorbic acid solution (Reagent B) and make up the
volume.
7. Run a blank sample (without soil) by following similar above steps.
Calculation:
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Olsen’s method is used for P estimation of neutral- alkaline soils
Reagents required:
• Reagent A:
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• Reagent B (Ascorbic acid solution): Dissolve 1.056 gm of ascorbic acid
in 200 ml of the molybdate solution (Reagent A) and mix it well.
Prepare it fresh as and when required.
Procedure:
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Calculation:
Available P (ppm) = Volume of BrayP1 solution added to soil x final volume(50) x Conc(ppm)
Instruments required
1. Flame photometer
2. Mechanical shaker
3. pH meter
Apparatus
1. Conical flask (100 ml)
2. Funnel and filter paper
3. Beaker (500ml)
4. Volumetric flask (100ml, 1000 ml)
Reagents:
1. Neutral normal ammonium acetate: Dissolve 77.08 gm of ammonium
acetate in about 800 ml of distilled water in one litre volumetric flask.
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Adjust the pH to 7.0 with dilute acetic acid and ammonia solution and
make the volume to one litre.
2. Standard K solution: Prepare 1000 ppm K solution by dissolving 1.908
gm of dried KCl salt in 1000 ml distilled water.
3. Working standard K solution: Dilute 0.5 ml, 1 ml, 1.5 ml and 2.0 ml of
1000 ppm K solution to 100 ml to get 5, 10, 15 and 20 ppm K solution.
Procedure:
1. Weigh 5 gm of soil in 100 ml conical flask
2. Add 25 ml of ammonium acetate solution and shake for five minutes on
mechanical shaker.
3. Filter the solution
4. Measure K concentration in the filtrate using flame photometer after
stadardization of the instrument with the help of potassium standard
solution.
Calculation:
Available Sulphur:
Apparatus and glassware:
1. Volumetric flask (1000 ml, 100ml, 25ml)
2. Beaker 500 ml and 100 ml
3. Conical flask (100ml)
4. Funnel
5. Filter paper
6. Pipette ( 25 ml, 10ml and 5ml)
7. Spectrophotometer
8. Mechanical shaker
Reagents:
1. 0.15% Cacl2 : Dissolve 1.5 gm of Cacl2.2H2O in about 500 ml of distilled water
and dilute it to 1000 ml.
2. 0.25% gum acacia solution: Dissolve 2.5 gm of gum acacia in 200 ml of warm
water and dilute it to one litre.
3. Barium chloride
Procedure:
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1. Weigh 5 gm of soil in 100 ml conical flask.
2. Add 25 ml of 0.15% Cacl2 and shake for half an hour on a mechanical shaker.
3. Filter the solution in a reagent bottle.
4. Pipette out 10-15 ml of the aliquot in 25 ml volumetric flask, to it add 1ml of
0.25% gum acacia solution.
5. Add approx. 1 gm of barium chloride and finally make up the volume up to 25
ml and take the reading at spectrophotometer at 440nm wavelength.
6. Run a blank (without soil) also by following the above steps.
Standard stock solution of sulphur (2000ppm): Dissolve 1.089 gm of AR grade
potassium sulphate in 100 ml distilled water.
Working standard sulphate solution (10 ppm): Dilute 5ml of 2000 ppm sulphur
solution to 1 litre
Preparation of standard curve: Pipette out 2.5, 5, 7.5,10 and 12.5 ml of 10 ppm
sulphur solution into 25 ml volumetric flask for 1,2,3,4 and 5 ppm sulphur solution.
To it add 1 ml of 0.25 % gum acacia solution and approx. 1 gm of barium chloride
and make up the volume to 25 ml. calibrate the spectrophotometer with the standard
sulphate solution and take the samples reading.
Calculation:
Available sulphur Kg/ha = ml of Cacl2 added to soil x Final volume x
Reading(ppm) x 2.24
Weight of soil vol.of aliquot
Available Boron
Apparatus and glassware:
1. Distillation flask and condensers
2. Reagent bottle
3. Funnel
4. Filter paper
5. Pipette
6. Measuring cylinder
7. Volumetric flask(25 ml, 100ml and 1000 ml)
8. Spectrophotometer
Reagents:
1. 0.01 M Cacl2 : Dissolve 1.47 gm of AR grade CaCl2 .2H2O in 200 ml
distilled water and make the volume one litre .
2. Buffer solution: Dissolve 250 gm of ammonium acetate and 15 gm of
EDTA ( Ethyline diamine tetra acetic acid) in 400 ml of distilled water
and add slowly 125 ml of glacial acetic acid.
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3. 1% L-Ascorbic acid solution: Dissolve 1 gm of L-Ascorbic acid
solution in 100 ml of distilled water.
4. Azomethine-H reagent: Dissolve 0.45 gm of azomethine –H in 100 ml
of 1 % L- ascorbic acid solution. Prepare a fresh reagent when it is
needed.
Procedure:
1. Weigh 20 gm of soil in a distillation flask and add 40 ml of
0.01MCacl2 solution.
2. Boil the content for 10 minutes
3. After cooling filter the solution in a reagent bottle
4. Run a blank also (without soil).
5. Pipette out 15 ml of filtrate in 25 ml volumetric flask
6. Add 2 ml of buffer solution and 2 ml of Azomethine-H solution
7. After half an hour take the reading on spectrophotometer at 420 nm
wavelength.
Preparation of standard curve:
1. Boron standard stock solution: Dissolve 0.570 gm boric acid in one
litre of distilled water to obtain a stock solution of 100 ppm.
2. Working standard B solution: Take 5 ml of the stock solution
(100ppm) in a 100 ml volumetric flask and dilute to 100 ml, this will
give rise to 5 ppm B solution.
3. Pipette out 0.5, 1.0, 1.5 and 2.0 ml of 5 ppm B solution into 25 ml
volumetric flask to get 0.1, 0.2, 0.3 and 0.4 ppm B solution
4. Add 2 ml buffer solution and 2 ml Azomethine-H solution and after
half an hour take the reading at 420nm on a spectrophotometer.
Calculation:
Available Boron (ppm) = ml of Cacl2 added to soil x Final volume x
Reading(ppm)
Weight of soil vol.of filtrate
Available Boron Kg/ha = Available boron (ppm) x 2.24
DTPA extractable Fe, Cu, Zn and Mn
Instruments
1. Mechanical shaker
2. Atomic absorption spectrophotometer (AAS)
Reagents
1. Dilute HCl: AR grade HCl diluted 5 times with double distilled water.
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2. DTPA extractant: Dissolve 1.967 gm of AR grade diethyline triamine
penta acetic
acid (DTPA) and 1.47 gm of Ca Cl2.2H2O(AR grade) in about 25 ml
of double
distilled water followed by add 13.3 ml of Trietanolamine (TEA).
Transfer the
solution to one litre volumetric flask. Just before making up the
volume, adjust pH
to 7.3 with dilute HCl. This reagent has 0.005M DTPA, 0.01M Ca
Cl2.2H2O and
0.1M Tri ethanol amine
Procedure
1. Weigh 10 gm of soil sample in 100 ml conical flask
2. Add 20 ml of the DTPA eatractant and shake for 2 hour on a
mechanical shaker.
3. Filter the solution through Whatman No. 1 filter paper
4. Feed the standard working solutions and prepare a standard curve by
plotting AAS reading against the element concentrations.
5. Measure the element concentration in the filtratet using AAS.
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1. Prepare 100 ppm Fe and Zn solution by diluting 10 ml of commercially
availabl 1000 ppm standard solution of Fe and Mn to 100 ml.
2. Prepare 25 ppm standard solution of Fe and Mn by diluting 25 ml of 100
ppm solution to100 ml.
3. Prepare working standard solution of 2.0, 4.0, 6.0 and 8.0 ppm of Fe and Mn
by diluting 8, 16, 24 and 32 ml of 25 ppm solution to 100 ml.
Precautions
Calculation:
APPENDIES
Appendix I
Percent concentration, specific gravity, Normality and amount needed for making 1N solution
of some commonly used liquid reagents
Reagents Conc. Sp. Gr.(gm/ml) Normality Quantity (ml)
(% w/w) at 20o C (Approx.) required for 1
Litre of 1N solution
Acetic acid glacial 99.0 1.06 17.5 58
Ammonium hydroxide 28.3 0.91 15.0 67
Hydrochloric acid 39.0 1.19 11.8 78
Nitric acid 71.0 1.42 15.6 62
Phosphoric acid 85.0 1.70 44.0 23
Sulphuric acid 96.0 1.84 36.0 28
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Appendix II
Some important conversion factors
N x 1.286 = NH4
N x 4.43 = NO3
Organic carbon x 1.724 = Organic matter
P x 2.29 = P2O5
K x 1.20 = K2O
Acre x 2.471 = Hectare = 2.24 x 106 Kg soil (Plough layer)
Milli equivalence per litre x equivalent weight = ppm
ppm (Parts per million) = micro gm per ml = mg per litre = mg per Kg.
mm per opening = 16/ mesh per inch
Appendix III
Rating chart for soil test values of primary and secondary nutrients
Nutrients Rating
________________________________
Low Medium High
Organic carbon (%) < 0.50 0.50 – 0.75 > 0.75
Available N (Kg/ha) < 280 281 – 560 >560
Available P (Kg/ha) < 10 11 -25 >25
Available K (Kg/ha) < 120 121 – 280 > 280
Available S (mg/Kg) < 10 10 -20 >20
Appendix IV
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Appendix- V
Calcium Dissolve 2.4973 gm of CaCO3 in 25 ml of 1 N HCl, and make the volume 1 litre
Potassium Dissolve 1.9067 gm of AR grade KCl in 100 ml distilled water and make the
volume 1 litre
Boron Dissolve 5.72 gm of Boric acid in 100 ml of warm distilled water and make the
volume 1 litre.
Iron Dissolve 1.00 gm of Fe metal (99.99% pure) in 100 ml of 3.5 N H2SO4 and make
the volume 1 litre.
Copper Dissolve 1.00 gm of Cu metal (99.99% pure) in 50 ml of 1:1 HNO3 and make the
volume 1 litre.
Manganese Dissolve 1.00 gm of Mn metal (99.99% pure) in 50 ml of 6 N HCl and make the
volume 1 litre.
Zinc Dissolve 1.00 gm of Zn metal(99.99% pure) in 50 ml of 1:1 HCl and make the
volume 1 litre.
Appendix-V
NUTRIENT RECOMMENDATIONS FOR DIFFERENT CROPS COMMONLY
GROWN IN JHARKHAND (Based on medium soil fertility status)
CROPS Nitrogen Phosphorus Potassium
Upland Rice 40 30 20
Rice (Medium land) 80 40 40
Rice Low land 150 60 90
Rice (Hybrid) 150 60 90
Maize(Composit) 120 60 40
Maize (Hybrid) 150 60 40
Ragi 40 30 20
Groundnut 25 50 20
Soybean 25 60 40
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Safflower 20 20 20
Niger 20 20 20
Kulthi/Ricebean 20 40 20
Arhar/Urd/Moong/ 20 40 20
Gram/Pea/Lentil
Arhar (Hybrid) 30 60 20
Wheat (Timely sown) 120 60 40
Wheat (Late sown) 80 60 40
Wheat (rainfed) 60 30 20
Rai/Toria 50 30 25
Linseed 20 20 20
Til 40 20 20
Potato 120 100 100
Cauliflower 100 60 60
Tomato 100 80 50
Pea(Green) 80 160 60
Frenchbean 40 80 40
Onion 120 60 100
Carrot/Raddish 100 50 75
Bhindi 100 50 50
Capsicum(HYV) 100 60 50
Capsicum(Hybrid) 200 100 400
Bottle gourd/Pumpkin/ 100 60 50
Sponge gourd
Bitter gourd 60 40 40
Parwal 120 60 50
Brinjal 100 50 50
Ginger 80 50 50
• If the soil test value falls under low category apply 25% more nutrients
• If the soil test value falls under High category apply 25% less nutrients
IMPORTANT FACTS & FIGURE
1.Common fertilizers & their nutrient content
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Fineness CaO
(mesh) %
When the land is ready for line sowing the furrow are opened at the
recommended distance for a particular crop. Powdered lime stone / dolomite/ basic
slag @ 2-3q ha-1 for desired area of land is applied in an open furrows. It is mixed with
soil by feet and than recommended dose of fertilizer is mixed with soil. Seeds are than
sown and covered with soil.
2.2 Preference of Crops : Pigeon pea, Soybean, Gram, Lentil, Peas, Moong, Urd,
Ground nut, Maize.
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Sl.No Name of Crop Dose Method of application
Organic
manure
1 FYM Pulse crop 1-2t/acre Soil application at the time of
sowing
Cerals 2-4 t/acre
Vegetable Crops 5-10t/acre
2 Compost Pulse crop 1-2t/acre Soil application at the time of
land preparation.
Cerals 2-4 t/acre
Vegetable Crops 5-10t/acre
3 Vermi Pulse crop 1t/acre Soil application at the time of
Compost land preparation.
Cerals 1-2 t/acre
Vegetable Crops 2-3t/acre
4 Neem cake Pulse crop 1-2q/acre Soil application at the time of
land preparation.
Cerals 2-4 q/acre
Vegetable Crops 5-10q/acre
5 Karanj Pulse crop 1-3q/acre Soil application at the time of
Cake land preparation.
Cerals 2-5 q/acre
Vegetable Crops 5-10q/acre
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5.0 Boi-fertilizers
27
7 Phosphogypsum NA
8 Borax NA
Organic Fertilizer Price (Rs)
9 Compost 40.0/q SSAC, BAU
Ranchi
10 Vermi Compost 5.0/kg Do
11 Neem cake 7.0/kg
12 Karanj Cake 7.0/kg
13 Rhizobium 15.0/100gr SSAC, BAU
Ranchi
14 Azotobactor 15.0/100gr Do
15 Azospirillum 15.0/100gr Do
16 Phosphate Solubilizing Bacteria 15.0/100gr Do
(PSB)
17 Blue Green Algae (BGA) 20.0/Kg Do
18 Azolla 20.0/Kg Do
19 Mycorhiza 20.0/Kg Do
Apply 75% NPK + 25% NPK through green manure or FYM at 6 t/ha
to rice and 75% NPK to wheat.
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Inoculation of BGA @ 10 kg/ha provides about 20-30 kg N/ha.
Rice-rice Use of organic sources, such as FYM, compost, green manure, azolla
etc. meet 25-50% of N needs in kharif rice and can help curtailing
NPK fertilizers by 25-50%.
Apply 75% NPK + 25% NPK through green manure or FYM at 6 t/ha
to kharif rice and 75% NPK to rabi rice.
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Groundnut NPK + Organic+ Rhizobium+lime
Vegetable NPK + Organic+ Boron as per soil test+lime
Crops
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14. Saraikela- Programme Coordinator
Kharsawan Krishi Vigyan Kendra, Gamharia (Block campus), P.O Gamharia
District : Saraikela- Kharsawan, Pin : 832 108
15. Bokaro Programme Coordinator
Krishi Vigyan Kendra, P.O- Paterwar (Near Block)
District : Bokaro, Pin : 829 121
16. Chatra Programme Coordinator
Krishi Vigyan Kendra, Seed Multiplication farm Kullu
District : Chatra, Pin : 825 401
17. Ranchi I/C soil Testing Department of Soil Science & Agriculture
chemistry BAU, Kanke, Ranchi
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