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User friendly microbial detection !

Sanita-kun R
=The ready-to-use cultural medium sheet
for the microbial detection =

Chisso Corporation
e-mail sanita-kun@chisso.co.jp
web http://www.chisso.co.jp/sanita/
Chisso Corporation

What is the SANITAKUN?

SANITAKUN is our original product Sheet Medium.

SANITAKUN is a ready-to use cultural medium sheet


equipped with non-woven fabric.

SANITAKUN provides easy and accurate detection of


microorganisms to customers as an alternative to agar plate.
Chisso Corporation
The structure of SANITAKUN

Water-soluble high polymer


Transparent
nutrients
cover film
chromogen
(oxygen permeability)
inhibitor
Non-woven fabric
Fabric pad

Adhesive film waterproof sheet

Apply 1ml sample solution

High viscous solution dissolves, it pushes up


incubate
microorganism to the fabric surface. The
Applied sample solution diffuses Microorganism grows and forms colony on the
throughout the entire pad in a few surface and it colors by chromogen.
seconds.
Chisso Corporation

SANITAKUN line-ups

SANITAKUN Chromogenic principal incubation


Chromogen(TTC) colors red by a microbial
Aerobic Count 35㷄 48hr
metabolism.
Chromogen colors blue or blue-green by
Coliform 35㷄 24hr
㱎-galactosidase that coliform generates.
Chromogen colors blue or indigo blue by
Yeast & Mold 25㷄 48hr
esterase that yeast or mold generates.
Chromogen colors black spot and blue halo
Staph. aureus 35㷄 24hr
around the spot.
Chromogen colors blue or blue-green by 㱎-galactosidase that
E.coli/Coliform coliform generates. Chromogen dyes indigo blue by 35㷄 24hr
㱎-glucuronidase that E.coli generates.
Chromogen colors blue by 㱍-galactosidase that salmonella
Salmonella generates. (Chromogen dyes indigo blue by 35㷄 24hr
㱎-galactosidase that Coliform generates.)
Chisso Corporation
SANITAKUN chromogenic characteristic

Aerobic Count Coliform Yeast & Mold

Staph. aureus E.coli/Coliform Salmonella


Chisso Corporation
Concept of SANITAKUN

User friendly Care free Reliable

1) Easy to use
2) Multi applications
3) Accurate & Quantitative
4) Reliability
5) Ready-to-use & Time saving
6) Space saving & Small volume of waste
7) Long validity
Chisso Corporation
Characteristics 1:
Easy to use
Direct stamping

Foods or swabbing sample


solution

Incubation
Characteristics 2 :
Chisso Corporation
Multi applications

Water filtrate
Swabbing Direct stamping

Fall out test


Swabbing test used
swabbing kit

Food sample test


Chisso Corporation
Characteristics 3 :
Accurate & Quantitative

SANITAKUN Aerobic Count

Incubation for 48h


8
y = 1.0338x - 0.2767
Sanita-kun Aerobic
Count(logcfu/mL)

0
0 2 4 6 8

Plate Count Agar(logcfu/mL)


Chisso Corporation SANITAKUN Aerobic count
Comparison between SANITAKUN Aerobic Count and
agar plate / AOAC Official Method 966.23
8

7
y = 0.9862x - 0.0197
2
R = 0.992
6
Sanita-kun (logcfu/g)
5

1
1 2 3 4 5 6 7 8

Plate Count Agar (logcfu/g)


Chisso Corporation Fresh produce (n=52) Sea foods (n=77)
8
8

SANITAKUN 6
6

Aerobic Count 4 4

2
2
(X;log agar plate,Y;log SANITAKUN) y = 1.0834x - 0.3872 y = 1.039x - 0.2873
0
35㷄,2days 0
0 2 4 6 8
0 2 4 6 8

Candy (n=83) Dairy (n=5) Grain(n=16) Meat(n=29)


8  6
8

6 4
6

4

4
2
2
2 y = 1.0044x + 0.0242

 0 0
0    0 2 4 6
0 2 4 6 8
0 2 4 6 8

Bean(n=6) Seaweed(n= 9) Daily dish(n=127) Egg(n=4)


8

4 6
6

4
4

2

y = 1.1242x - 0.4385 2 2
y = 1.0194x - 0.2028
[Z  y = 1.1297x - 0.5192
0  0 0
0 2 4 0 2 4 0 2 4 6 8 0 2 4 6
Chisso Corporation

SANITA-KUN Coliform

Comparison between SANITAKUN and agar

㪏 㪏
㫐㩷㪔㩷㪇㪅㪐㪋㩷㫏㩷㪂㩷㪇㪅㪉㪐

SanitaKun Coliform (logcfu/g)


SanitaKun Coliform (logcfu/g)

㫐㩷㪔㩷㪇㪅㪐㪌㩷㫏㩷㪂㩷㪇㪅㪉㪊

䉰䊆ᄥ䈒䉖ᄢ⣺⩶⟲↪㩿㫃㫆㪾㪺㪽㫌㪆㪾㪀

㫉㪔㪇㪅㪐㪌 㪎

䉰䊆ᄥ䈒䉖ᄢ⣺⩶⟲↪㩿㫃㫆㪾㪺㪽㫌㪆㪾㪀
㫉㪔㪇㪅㪐㪌
㪍 㪍

㪌 㪌

㪋 㪋

㪊 㪊

㪉 㪉

㪈 㪈
㪈 㪉 㪊 㪋 㪌 㪍 㪎 㪏 㪈 㪉 㪊 㪋 㪌 㪍 㪎 㪏
Desoxycholate agar(logcfu/g)
䊂䉸䉨䉲䉮䊧䊷䊃ኙᄤၭ࿾㩿㫃㫆㪾㪺㪽㫌㪆㪾㪀
X-Gal agar(logcfu/g)
㪯㪄㪞㪸㫃ኙᄤၭ࿾㩿㫃㫆㪾㪺㪽㫌㪆㪾㪀
Chisso Corporation SANITA-KUN Yeast & Mold (Rapid Type)
Comparison between SANITAKUN and PDagar


㪤㪩㩷㪉㪻㪸㫐㫊
㪪㪸㫅 㫀㫋㪸㫂㫌㫅 㩷㪰㩽㪤㩷㩿㪤㪩㪀䇭䌬䌯䌧䌣䌦䌵 㪤㪩㩷㪊㪻㪸㫐㫊


㪇 㪈 㪉 㪊 㪋 㪌 㪍 㪎
㪧㫆㫋㪸㫋㫆㩷㪻㪼㫏㫋㫆㫉㫆㫊㪼㩷㪸㪾㪸㫉㩷㪎㪻㪸㫐㫊䇭䌬䌯䌧䌣䌦䌵
Chisso Corporation
Reliability
Characteristics 4 :

The international certification


•SANITAKUN Aerobic Count, Coliform were approved by
AOAC Research Institute as the Performance Tested Method.
•SANITAKUN E.Coli/Coliform was approved by AOAC
Research Institute as the Performance Test Method on this
July
•We will try to apply a validation such a AOAC PTM approval
about SANITAKUN other item, then too.

The official recognition in Japan


SANITAKUN was appeared on the Food Sanitation Inspection
Guide Line which official guide line of Japan.
( Aerobic Count, Coliform, Staph. aureus, E.coli/Coliform )
Chisso Corporation
Characteristics 5:
Ready-to-use & Time saving

Comparison of preparation time between


SANITAKUN and agar plate (100 tests)

0 1 2 3 48 hours
Time

Start Sample application


autoclaving
Agar
measuring cooling incubation

Start
Sample application
SANITAKUN

We can save 3 hours using SANITAKUN


Chisso Corporation

Characteristics 6:
Space saving 䋧 Small volume of waste
10 agar plates

10 SANITAKUNs sheets
Space saving

䋱䋯䋱䋰
to agar plates

20 autoclaved plates䋨2.2 400 autoclaved


litters䋩 SANITAKUNs sheets

Small volume of
waste
䋱䋯䋲䋰 䋽
to agar plates
Chisso Corporation
Characteristics 7:
Long validity

Sanita-kun validity (M)


Aerobic count 24
Coliform 36
Yeast & Mold 24
Staph. aureus 24
E.coli/Coliform 36
Salmonella 36
Validity from manufacturing date in a stock condition of tight sealing
in a refrigerator.
Chisso Corporation

SANITA-KUN’s field
Food processing
Convenience store’s vender
Healthy foods
Poultry & Meat Industries
Fish Industries
Hotels & Restaurants

䊶䊶䊶䊶 and Others


Cosmetic
Water supply
school lunch etc

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