2-Varian 2005 Sample Prep Web

Varian Sample Preparation Products 1
Quality and Commitment for Over 25 Years

For more than 25 years, Varian has been solving sample preparation challenges with
pioneering techniques and dedicated customer support. Varian introduced the original
straight-walled Bond Elut SPE cartridge in 1979 that has now become the standard format
for solid phase extraction (SPE). This innovation has continued with the development of
novel chemistries and formats that make it simpler, easier, and faster to perform sample
preparation. The most recent development is FOCUS, a new SPE technology that provides
cleaner extracts and enhanced polar recovery.
Today, Varian has an unparalleled offering of products for sample preparation. Whether you
need packed bed or disc; polymer or silica; cartridge, 96-well plate or pipette tip, Varian
has the solution for you.
Preparation
Sample
A Continuing History of Innovation
• Bond Elut: the original packed-bed SPE. Over 40 bonded phases are available in a wide range of cartridge formats to meet your sample requirements.
• Chem Elut: the first extraction cartridge to dramatically simplify liquid/liquid extraction. Now available in 96-well format.
• VersaPlate: brings flexibility to 96-well SPE. A modular design with removable tubes eliminates waste and streamlines the method development process.
• Captiva: unique filtration products in 96-well, 3 mL and 10 mL formats fulfill your bioanalysis sample preparation needs, such as filtration of
precipitated plasma proteins.
• MetaFlash: high quality Flash Chromatography products provide superior performance and are stocked for fast delivery.
• SPEC: trouble-free SPE. Unique monolithic disc with enhanced flow characteristics is ideal for trouble-free, high-throughput sample processing.
• FOCUS: Polar-Enhanced SPE. Unique new chemistry enhances recoveries of polar analytes and provides cleaner extracts.
• TOP: a simple, cost-effective, high-throughput solution for Trityl-on Oligonucleotide Purification.
Contact your Varian, Inc. sales representative for a free copy, or use Scanview
online at www.varianinc.com, under Consumables, click Applications Online.
See All of Our Products, and Shop Online at www.varianinc.com

2 Sample Preparation Product Selection Guide
GC—LC—MS—AAS—ICP—UV-Vis-NIR—FT-IR—Fluorescence—Dissolution—NMR—Consumable Products
Sorbent Specifications 3
Typical Surface Particle Mean Mean
Bonded Functional Group/ Base
Sorbent Phase Category Endcapped Format Carbon area Size and Pore Size pKa Capacity
Material
Loading m2/g Shape Å meq/g
40 and
Sulfonic acid (SCX) and Quaternary Packed
AccuCAT Mixed Mode No 7.0% 500 120 µm, 60
amine (SAX)/ Silica based bed
irregular
Packed
Alumina (AL-A) Polar Aluminum Oxide - acidic N/A 0% N/A 25
bed
Packed
Alumina (AL-B) Polar Aluminum Oxide - basic N/A 0% N/A 25
bed
Packed
Alumina (AL-N) Polar Aluminum Oxide - neutral N/A 0% N/A 25
bed
40 and
Aminopropyl Polar/Anion Packed
Aminopropyl/Silica based No 6.7% 500 120 µm, 60 9.8 1.1
Preparation
(NH2) Exchanger bed
irregular
Sample
SPEC Aminopropyl Polar/Anion
Aminopropyl/Silica based No SPEC Disc 220 N/A 70
(NH2) Exchanger
40 and
Application Trifunctional Octadecyl/ Packed
Atrazine Yes 11.8% 500 120 µm, 60
Specific Silica based bed
irregular
40 and
Trifunctional Octadecyl/ Packed
BioBond Elut Nonpolar Yes 6.0% 500 120 µm, 500
Silica based bed
irregular
Packed 40,
C1 Nonpolar Methyl/Silica based Yes 4.1% 500 60
bed irregular
40 and
Packed
C2 Nonpolar Ethyl/Silica based Yes 5.6% 500 120 µm, 60
bed
irregular
SPEC C2 Nonpolar Dimethyl/Silica based No SPEC Disc 2.7% 220 N/A 70
40 and
Packed
C8 Nonpolar Octyl/Silica based Yes 12.2% 500 120 µm, 60
bed
irregular
SPEC C8 Nonpolar Octyl/Silica based Yes SPEC Disc 5.0% 220
40 and
Trifunctional Octadecyl/ Packed
C18 Nonpolar Yes 17.4% 500 120 µm, 60
Silica based bed
irregular
Monofunctional Octadecyl/Silica
SPEC C18 Nonpolar No SPEC Disc 8.0% 220 N/A 70
based
Trifunctional Octadecyl/
SPEC C18 AR Nonpolar Yes SPEC Disc 9.0% 220 N/A 70
Silica based
Trifunctional Octadecyl/ Packed 40 µm,
C18 EWP Nonpolar Yes 6.0% 80 500
Silica based bed irregular
C18 INT Nonpolar Yes 13.0% 500 60
C18 LO Nonpolar Yes 11.8% 500 60
40 and
Monofunctional Octadecyl/Silica Packed
C18 OH Nonpolar No 14.9% 300 120 µm, 150
based bed
irregular
40 and
Cation Packed
CBA Carboxylic acid/Silica based Yes 7.4% 500 120 µm, 60 4.8 0.4
Exchanger bed
irregular
40 and
Octyl and benzenesulfonic acid (SCX)/ Packed
Certify Mixed Mode No 9.0% 500 120 µm, 60
Silica based bed
irregular
40 and
Octyl and quaternary amine (SAX)/ Packed
Certify II Mixed Mode No 8.6% 500 120 µm, 60
Silica based bed
irregular
40 and
Packed
CH Nonpolar Cyclohexyl/Silica based Yes 9.6% 500 120 µm, 60
bed
irregular
40 and
Packed
Cyano (CN-E) Nonpolar Cyanopropyl/Silica based Yes 8.1% 500 120 µm, 60
bed
irregular
40 and
Packed
Cyano (CN-U) Polar Cyanopropyl/Silica based No 7.8% 500 120 µm, 60
bed
irregular
SPEC Cyano Polar Cyanopropyl/Silica based No SPEC Disc 220 N/A 70

4 Sorbent Specifications
Typical Surface Particle Mean Mean
Sorbent Bonded Functional Group/ Base
Category Endcapped Format Carbon area Size and Pore Size pKa Capacity
Phase Material
Loading m2/g Shape Å meq/g
Application
SPEC DAU Silica based SPEC Disc N/A 220 N/A 70
specific
40 and
Diethylaminopropyl/ Packed
DEA Anion Exchanger No 8.5% 500 120 µm, 60 10.7 1.0
Silica based bed
irregular
Packed 40 µm,
Diol (2OH) Polar Diol/Silica based No 6.8% 500 60
bed irregular
Packed 125,
ENV Nonpolar Styrene Divinylbenzene N/A N/A 450
bed spherical
40 and
EnvirElut Application Trifunctional Octadecyl/ Packed
No 18.0% 500 120 µm, 60
1664 Specific Silica based bed
irregular
Packed
FL Polar Florisil N/A N/A 200 --
bed
Polar-enhanced Polar functionalized Packed 44 µm,
FOCUS N/A 640 120
styrene divinylbenzene bed spherical
Packed 75 µm,
LMS Nonpolar Styrene Divinylbenzene N/A N/A 300
bed spherical
Nonpolar and benzenesulfonic acid
SPEC MP1 Mixed Mode SPEC Disc 6.0 220 N/A 70
(SCX)/Silica based
Slightly polar and benzenesulfonic
SPEC MP3 Mixed Mode SPEC Disc 220 N/A 70
acid (SCX)/Silica based
Packed 70 µm, 100/450
NEXUS Mixed mode Mixed Mode Copolymer N/A 575
bed spherical Bimodal
Packed 40 µm,
PBA Covalent Phenylboronic acid No 7.9% 500 60
bed irregular
Application Packed
PCB Layered Phase N/A N/A 500 N/A N/A
Specific bed
40 and
Packed
PH Nonpolar Phenyl/Silica based Yes 10.7% 500 120 µm, 60
bed
irregular
SPEC PH Nonpolar Phenyl/Silica based Yes SPEC Disc 220 N/A 70
Packed 125 µm,
PPL Nonpolar Functionalized Styrene Divinylbenzene N/A N/A 600 150
bed spherical
Propylsulfonic acid/ Packed 40 µm,
PRS Cation Exchange No 1.7% 500 60 <1.0 0.18
40 and 10.1
Packed
PSA Anion Exchanger Ethylenediamine-N-propyl/Silica based No 7.5% 500 120 µm, 60 & 1.40
bed
irregular 10.9
SPEC PSA Anion Exchanger Ethylenediamine-N-propyl/Silica based No SPEC Disc 220 N/A 70
40 and Quater-
Trimethylaminopropyl/ Packed
SAX Anion Exchanger No 7.5% 500 120 µm, 60 nary 0.85
Silica based bed
irregular
Trimethylaminopropyl/
SPEC SAX Anion Exchanger No SPEC Disc 220 N/A 70
Silica based
40 and
Benzenesulfonic acid/ Packed
SCX Cation Exchanger No 10.9% 500 120 µm, 60 <1.0 0.79
Silica based bed
irregular
Benzenesulfonic acid/
SPEC SCX Cation Exchanger No SPEC Disc 220 N/A 70
Silica based
40 and
Packed
SI Polar Silica No N/A 600 120 µm, 60
bed
irregular
SPEC SI Polar Silica No SPEC Disc 220 N/A 70
40 and
Application Packed
TCA Ethyl/Silica based Yes 500 120 µm, 60
Specific bed
irregular
Application Packed 125 µm,
TOP Functionalized Styrene Divinylbenzene N/A 600 150
Specific bed spherical
Sorbent Selection Guide 5
AccuCAT Aminopropyl (NH2)
Bond Elut AccuCAT cartridges are mixed-mode SPE columns consisting of • Ideal for structural isomer fractionation
a strong cation exchange and a strong anion exchange sorbent packed • Anion exchange and polar retention mechanisms
into one bed. AccuCAT is effective for the extraction of acidic, basic, and • Weak nonpolar retention
neutral compounds from urine and other biological samples. AccuCAT is
particularly effective for catecholamine extraction from biofluids.
Alumina-A (AL-A)
• Acidic Alumina (Al2O3) Aminopropyl (NH2), a polar sorbent, utilizes both hydrogen bonding and
• pH: 4.5 anion exchange. With a pKa of 9.8, NH2 is a weaker anion exchanger
• Particle size: 25 µm than sorbents such as SAX (a quaternary amine sorbent that is always
Preparation
• Primary retention mechanism: Lewis acid/base, polar, and ion exchange charged) and is therefore a better sorbent choice for retention of very
Sample
strong anions, such as sulfonic acids, which may retain irreversibly on SAX.
Because a propyl tether supports the NH2 functionality, it can be used for
nonpolar isolations from polar samples, but its strong polarity is its primary
characteristic. NH2 is excellent for separation of structural isomers.
Alumina is a classic Lewis acid, lacking two electrons in the aluminum
center. The Lewis acid properties are enhanced in Alumina-A, making the Atrazine
sorbent more retentive towards electron-rich compounds. This sorbent has
a slightly cationic nature through pretreatment with acidic solutions. The Bond Elut Atrazine is specially designed for the extraction of
result is a surface that favors neutral and anionic species (e.g. neutral acid Atrazine and its desalkylated metabolites -- des-ethylatrazine and
or acid anion), while discouraging retention of cationic species. Weak ion desisopropylatrazine -- from water samples. The sorbent is based on
exchange is also possible with anionic compounds. Bondesil C18-LO, with carefully controlled carbon content to ensure
reproducibly high recoveries of Atrazine and its polar metabolites.
The bigger particle size of the sorbent and the diameter of the tube
Alumina-B (AL-B) allow fast flow for large volume water samples. And, the large bed
mass of the Bond Elut Atrazine phase has been optimized to provide
• Basic Alumina (Al2O3)
enough capacity to ensure good recovery of Atrazine and its polar
• pH: 10.0
metabolites. With the use of the Bond Elut Atrazine extraction cartridge
• Particle size: 25 µm
and optimized method (M2638) in combination with a Varian HPLC
• Primary retention mechanism: Lewis acid/base, polar, and ion-exchange
diode array detection system, quantitative recoveries of Atrazine and its
desalkylated metabolites in surface water have been achieved, even at
Alumina-B has a surface that favors retention of cationic or hydrogen-
extremely low concentrations (0.02 ppb). These concentration levels are
bonding species. This sorbent has an anionic nature through the
far below the tolerance level specified by the EPA (3 ppb) and by those
pretreatment of the alumina with basic solutions. The result is a surface
set by the most stringent European agencies (0.1 mg/L and 0.5 mg/L). The
that favors cation exchange, though the surface also exhibits some
extraction and analysis methods are fast, reproducible, and require minimal
Lewis base properties and therefore can also retain electron-donors like
organic solvent consumption.
neutral amines. However, this capacity is much lower than for the neutral
and acidic aluminas. Strong hydrogen bonding is possible on Alumina-B,
making it particularly effective for polar cations. BioBond Elut
Choose BioBond Elut for fast, simple desalting of synthetic
Alumina-N (AL-N) oligonucleotides from final deprotection reaction mixtures. With
BioBond Elut there are no minimum molecular weight limits, so oligos
• Neutral Alumina (Al2O3)
less than 5,000 Daltons can be desalted with >98% recoveries. And, while
• pH: 7.5
typical desalting media can leave as much as 2% of the original salt in your
sample, BioBond Elut provides 100% salt-free samples.
• Primary retention mechanism: Lewis acid/base, polar, and ion exchange
Alumina, like silica, is an extremely polar sorbent. The alumina

surface tends to be slightly more stable under high pH conditions than
unfunctionalized silica. The smaller particle size ensures high extraction
efficiency and small (50 mg) sorbent beds are therefore commonly used.
This sorbent has an electrically neutral surface that favors the retention
of electron-rich compounds like aromatic species and aliphatic amines. It
also favors retention of compounds containing electronegative groups (for
example, functional groups with oxygen, phosphorus and sulfur atoms). It
may be used in extracting either nonpolar or polar species from aqueous
or nonaqueous samples respectively.

6 Sorbent Selection Guide
C1 C18
• Bonded functional group: methyl • Bonded functional group: trifunctional octadecyl
• Primary retention mechanism: very weakly nonpolar • Primary retention mechanism: strongly nonpolar
• Typical sample types: plasma, urine, and aqueous samples • Typical sample types: water, aqueous biological fluids
Due to the single carbon functional group, Bond Elut C1 is the least
Bond Elut C18 is the most hydrophobic silica-based sorbent available. It is the
retentive of all alkyl-bonded phases for nonpolar compounds. However,
most popular SPE sorbent because of its extreme retentive nature for non-
because the sorbent is endcapped, silanol activity is generally masked.
polar compounds. C18 is generally regarded as the least selective silica based
The result is easier retention and elution of polar and multifunctional
sorbent, because it retains most organic analytes from aqueous matrices —
compounds.
often a benefit when the analytes vary widely in structure. The potential
for polar interactions with analytes is less significant with C18 than
C2 with any other sorbent because of the predominant effect of the long
hydrocarbon chain. When extracting small- to intermediate-sized
• Bonded functional group: ethyl molecules, C18 can also be utilized for desalting prior to ion exchange
• Primary retention mechanism: weakly nonpolar because salts pass through it unretained.SPEC C18 is a nonendcapped,
• Typical sample types: plasma, urine, and aqueous samples monofunctional version of the octadecyl bonded phases and offers similar
selectivity to Bond Elut C18-OH.
C18 AR
• Bonded functional group: trifunctional octadecyl
Bond Elut C2 is generally considered more polar than longer-chain • Primary retention mechanism: strongly nonpolar
alkyl-bonded sorbents. The short C2 alkyl group provides a smaller area • Typical sample types: water, aqueous biological fluids
for nonpolar interactions to occur. C2 is often used as a replacement
for C18 and C8 when molecules are retained too strongly by the latter.
C2’s polarity is slightly lower than CN for polar interactions. It is popular
for the extraction of drugs from plasma and serum because of the very
flat chromatographic baselines that can be achieved with appropriate
wash and elution conditions.SPEC C2 is a monolithic disk featuring an
unendcapped dimethyl bonded functional group. SPEC C2, therefore, SPEC C18AR (acid resistant) is an endcapped, trifunctional version of the
offers a different selectivity than Bond Elut C2. octadecyl phases and offers similar selectivity to Bond Elut C18. C18AR is
exclusively available in SPEC disc format.
C8
C18 EWP
• Bonded functional group: octyl
• Primary retention mechanism: moderately nonpolar • Bonded functional group: trifunctional octadecyl
• Typical sample types: water, aqueous biological samples • Primary retention mechanism: strongly nonpolar
• Typical sample types: water, aqueous biological fluids
Bond Elut C8 is very similar in properties to C18, but is not as retentive for
nonpolar compounds due to its shorter hydrocarbon chain. C8 is a good Bond Elut C18 EWP (extra wide pore) sorbent has 500 Å pores to allow more
replacement for C18 when analytes are too strongly retained on C18 for efficient extraction of large molecules (> 15,000 MW). Macromolecules of
effective elution. The potential of C8 for polar interactions with analytes this size are typically excluded from standard porosity phases, but with
is somewhat higher than for C18 because the shorter hydrocarbon chain C18 EWP successful separation of complex mixtures of proteins, peptides
does not mask the silica surface as effectively, but polar interactions are or nucleotides is achieved. C18 EWP is a good sorbent for desalting large
still not a significant property of C8. The C8 sorbent has been successfully molecular weight compounds prior to LC/MS or LC/MS/MS, capillary
utilized in the simultaneous extraction of fat- and water-soluble vitamins electrophoresis, or any other analytical technique that is adversely affected
from human serum. by the presence of salts in the sample.
SPEC C8 offers similar selectivity to Bond Elut C8.
C18 INT Carboxylic Acid (CBA)
• Bonded functional group: trifunctional octadecyl • Bonded functional group: carboxylic acid
• Primary retention mechanism: strongly nonpolar • Primary retention mechanism: weak cation exchange
• Typical sample types: water, aqueous biological fluids • Best choice for extraction of strong cations
• Typical sample types: water, biological fluids
Bond Elut C18 INT (intermediate carbon loading) is similar in properties

Bond Elut CBA is a medium polarity sorbent based on carboxy-
to the standard Bond Elut C18 sorbent, but has a slightly lower carbon
propyl-functionalized silica, and exhibits either polar or nonpolar
Preparation
loading. This intermediate carbon load C18 is thoroughly endcapped,
interactions depending on the matrix or solvent environment. Its primary
Sample
ensuring rapid release of basic drugs. The carbon loading has been
characteristic is its weak cation exchange ability. Because of its pKa of
optimized to differentiate between metabolites of classes of compounds
4.8, CBA does not require the high basic conditions for elution that are
such as prostaglandins. In addition, the narrow pore size helps exclude
required by strong cation exchange sorbents. Simply, lowering the pH to
large molecules such as proteins.
2.8 effectively neutralizes CBA, permitting elution of the retained analytes.
For this reason, CBA is often the best choice for extraction of very strong
C18 LO cations.
• Low carbon loading gives unique C18 selectivity
• Strongly nonpolar retention mechanism Certify
• Ideal for polar analyte extraction from water samples and
biological fluids • Bonded functional group: mixed-mode sorbent bed, octyl (C8) and
benzenesulfonic acid (SCX)
• Primary retention mechanism: nonpolar and strong cation exchange
• Ideal for forensic and drugs of abuse analyses
• Typical sample types: urine, biological fluids
Bond Elut Certify is the original mixed-mode sorbent, designed for the
C18 LO, like C18 and C18 INT, is a highly retentive nonpolar silica-based extraction of basic (cationic) drugs from urine, plasma, serum, whole blood
sorbent due to the long hydrocarbon chain. However, the low carbon and other biological matrices. Examples of drugs and drug classes that can
loading of C18 LO gives unique selectivities over other octadecyl sorbents. be extracted with Certify include:
The carbon loading has been optimized for polar analyte extraction from • Basic and nonpolar pharmaceutical compounds
environmental matrices. Quantitative recoveries of Atrazine metabolites • HC metabolite, 11-nor-delta-9-tetrahyrdocannabinol-9- carboxylic acid
have been successfully achieved with C18 LO sorbents. (Note: see Bond Elut • Cocaine and benzoylecognine
Atrazine section for optimized bed mass and application information.) • Amphetamine and methamphetamine
• Phencyclidine
C18 OH • Opiate and 6-MAM
• Barbiturates
• Bonded functional group: monofunctional octadecyl • Benzodiazepines
• Primary retention mechanism: moderately nonpolar, plus significant • Acid/base/neutral screens
polar secondary interactions
• Typical sample types: biological matrices, aqueous samples Analytes are strongly retained on Certify by a combination of ion exchange
and nonpolar mechanisms. This allows the use of aggressive aqueous and
organic washes to rinse away nonpolar, polar and ionic matrix contami-
nants. The result is outstanding sample clean up and concentration of
samples prior to screening or confirmation by GC, GC/MS, LC/MS or TLC.
SPEC MP-1 offers similar selectivity to Bond Elut Certify.

Bond Elut C18 OH is a non-endcapped version of the octadecyl bonded
phases that enable the silanols on the silica surface to be more active. This
low-load C18 has controlled silanol activity under tight QC specifications
for superior batch-to-batch reproducibility. The silanol activity permits
fractionation of metabolites and enhances retention of basic compounds
compared with endcapped C18. In addition, the 150Å intermediate pore
size is ideal for intermediate molecular weight compounds that might not
be retained on a standard 60Å material.SPEC C18 offers similar selectivity
to Bond Elut C18 OH.

Certify II Cyanopropyl (CN-U)
• Bonded functional group: mixed-mode sorbent bed; octyl and • Bonded functional group: unendcapped cyanopropyl
quaternary amine (SAX) • Primary retention mechanism: weakly nonpolar (aqueous matrix) or
• Primary retention mechanism: nonpolar and strong anion exchange polar (nonpolar organic matrix)
• Typical sample types: urine, biological fluids • Typical sample types: nonpolar solvents, organic extracts of aqueous
sample
Bond Elut Certify II was developed specifically for the rapid and effective
extraction of acidic drugs and metabolites from urine and other biological
matrices. Certify II is a mixed-mode cartridge packed with nonpolar C8 and
strong anion exchange (SAX) sorbent. It has been optimized for extraction
of acidic and nonpolar drugs such as THC metabolite and nonsteroidal
anti-inflammatory drugs. Unendcapped Bond Elut CN-U is a medium polarity sorbent. It is less
retentive than Si or 2OH for very polar analytes that might be retained
irreversibly on these more polar sorbents. The unendcapped cyano phase
Cyclohexyl (CH) is ideally suited for the extraction of polar compounds from a nonpolar
matrix such as hexane or oils.
• Bonded functional group: cyclohexyl
• Primary retention mechanism: moderately nonpolar
SPEC CN offers similar selectivity to Bond Elut CN-U.
• Typical sample types: water, aqueous samples
DAU
SPEC DAU is specifically designed for the analysis of Drugs of Abuse
in urine. Based on the monolithic silica extraction disc, SPEC DAU is
Bond Elut CH is a medium polarity sorbent that exhibits unique selectivity ideally suited for high-throughput automation. Its unique sorbent
in nonpolar extractions. When employed as a nonpolar sorbent, CH has the chemistry results in outstanding sample cleanup and concentration of
approximate polarity of a C2 sorbent. The polar subsurface is an important samples prior to screening or confirmation by GC, GC/MS, LC/MS or TLC.
factor among CH properties, as indicated by the ability of CH to retain
polar analytes such as phenol from aqueous matrices. Because of its unique Diethylaminopropyl (DEA)
selectivity, CH is often a good choice when other nonpolar sorbents do not
provide the desired selectivity. • Bonded functional group: diethylaminopropyl
• Primary retention mechanism: weak anion exchange aqueous matrix)
or polar (nonpolar organic matrix)
Cyanopropyl (CN-E) • Typical sample types: water, biological fluids, nonpolar extracts
• Bonded functional group: endcapped cyanopropyl
• Primary retention mechanism: moderately nonpolar (aqueous matrix)
or polar (nonpolar organic matrix)
• Typical sample types: aqueous (nonpolar), organic (polar)
Bond Elut DEA bears some resemblance to PSA and NH2 in its properties. It
has slightly lower capacity as an anion exchange sorbent (1.0 meq/g), and
a more nonpolar character due to a longer alkyl tether. DEA is somewhat
more polar than C8, but less polar than C2 or CN.
Bond Elut CN-E is a medium polarity sorbent ideal for extraction of

extremely nonpolar compounds that irreversibly retain on more nonpolar Diol (2OH)
sorbents such as C18 or C8. The endcapped version of the cyano sorbent is
best utilized when extracting analytes from an aqueous matrix. • Polar sorbent with better reproducibility than silica
• Ideal for prostaglandin separation and other fractionations
• Typical sample types: nonpolar organic extracts, oils, and lipids
Diol (20H) is typically used for polar extractions from nonpolar solvents.
Diol resembles unbonded silica in its tendency for strong hydrogen bonding.
It also shares silica’s important ability to discriminate between similar
compounds such as structural isomers. Prostaglandins, for example, can be
separated into classes using Diol by slightly varying the solvent polarity. Diol
is a more predictable sorbent than silica for extraction methods requiring
a polar sorbent conditioned with a polar solvent. Diol can also be used for
nonpolar retention by virtue of its hydrocarbon spacer.
ENV FOCUS
• Sorbent description: polymeric wide-pore SDVB • Sorbent description: polar-enhanced SDVB polymer
• Typical applications: environmental, explosives, pharmaceuticals • Primary retention mechanism: polar and nonpolar
• Power Rinse™ delivers cleaner extracts/reduced ion suppression
Bond Elut ENV is a high purity styrene-divinylbenzene (SDVB) polymer • Typical sample types: pharmaceutical bioanalysis
optimized for the extraction of polar organic residues, such as herbicide
metabolites and explosives, from large volume water samples. Bond Elut FOCUS features a polar-enhanced sorbent technology that delivers
ENV features a 125 µm highly cross-linked spherical polymer that can outstanding recovery for polar as well as nonpolar analytes. Compared to
process 1 L water samples in a fraction of the time required by other other polymeric sorbents, FOCUS exhibits a unique hydrogen bond donor
sample preparation methods. The absence of secondary interactions on capability that significantly increases the retention of basic analytes. This
the polymer surface makes it easier to elute a wide range of nonpolar increased retention results in cleaner extracts and higher recoveries across
compounds with just one elution solvent. Although primarily used for a broad spectrum of drugs. The extraction method is simple, universal, and
Preparation
environmental applications, Bond Elut ENV can also be used for clinical eliminates the complex pH adjustments necessary with ion exchange and
Sample
applications, including the extraction of highly polar metabolites, such as mixed mode methods.
glucuronide-bound molecules from biological fluids.
LMS
EnvirElut 1664
• Sorbent description: highly cross-linked SDVB polymer
• Specifically designed for EPA Method 1664 - Oil and Grease extraction • High, controlled surface area for consistent extraction efficiency
from water • Typical sample types: biological samples such as urine, plasma, or serum
• High-flow - 1 L of sample in less than 10 minutes
• Manifold simplifies sample loading Bond Elut LMS allows elution without organic modifiers, buffers, or acids.
The absence of secondary silanol interactions allows elution of analytes with
EnvirElut 1664 offers a simple, rugged and cost effective solution for the pure organic solvents or HPLC mobile phase-compatible solvent mixtures of
processing of oil and grease samples. EnvirElut cartridges consistently pass low ionic strength. These characteristics ensure compatibility with LC/MS
1 liter of dirty water in less than 10 minutes, without loss or analyte, and and other analytical techniques. Featuring an optimized particle size range
deliver greater than 90% recovery with standards from 5 to 1,000 mg/L. and a large surface area for extraction efficiency, Bond Elut LMS provides
The procedure is totally Freon - free. When combined with Varian’s excellent recovery and reproducibility. LMS ensures worry-free use and can
AquaSep cartridges for moisture removal, EnvirElut 1664 offers a solid help reduce costly instrument downtime.
phase- extraction procedure with results equivalent to EPA 1664/ASTM
method 413.1 (gravimetry).
MP1
Florisil • Bonded functional group: mixed-mode SPEC disc: nonpolar/
• Sorbent description: MgO3Si • Primary retention mechanisms: nonpolar and strong cation exchange
• Primary retention mechanism: polar • Typical sample types: urine, biological fluids
• Typical sample types: environmental, organic extracts
SPEC MP-1 is a mixed-mode, monolithic disc format sorbent that delivers
superior flow and reproducible recoveries. SPEC MP-1 is ideal for analyzing
drugs with polar functional groups from plasma using LC/MS/MS. The dual
retention mechanism results in cleaner extracts. During the extraction,
Florisil is a magnesia-loaded silica gel. Like silica, it is extremely polar polar basic drugs are strongly held by the benzenesulfonyl group while
in nature and ideal for the isolation of polar compounds from nonpolar rigorous wash steps can be applied to remove the matrix components that
matrices. The larger particle size – 200 µm – of the Florisil material enables cause ion suppression.
fast flow of large-volume samples and thus is a potential alternative to
silica when extracting viscous samples. Florisil is ideal for the separation of Bond Elut Certify offers similar selectivity to SPEC MP-1.
chlorinated pesticides, and is used in numerous AOAC- and EPA- regulated
methods. Florisil has also been used as a replacement for Alumina in cases
where the Lewis-acidity of alumina interferes with the extraction. MP3
• Bonded functional group: mixed-mode SPEC disc: slightly polar/
• Primary retention mechanism: slightly polar and strong cation
exchange
• Typical sample types: urine, biological fluids
SPEC MP-3 is slightly more polar than MP-1, making MP-3 ideal for
hydrophobic analytes that would bind too strongly to MP-1. MP-3 is
especially suited for the extraction of opiate alkaloids.

NEXUS Phenyl (PH)
• Unique, ultraclean copolymeric material • Bonded functional group: phenyl
• No conditioning needed for hydrophobic analytes • Primary retention mechanism: moderately nonpolar
• High flow for viscous samples • Typical sample types: water, biological fluids
• Ideal for veterinary sciences and environmental applications
Nexus provides simple, clean SPE on a unique polymeric sorbent. It offers

a nonpolar retention mechanism with no conditioning required, enabling
increased throughput or improved ease of field sampling in environmental
applications. Nexus delivers reproducibly high recoveries. This performance Bond Elut PH is most commonly employed for nonpolar extractions, with
is achieved from the use of a proprietary, highly cross-linked, spherical a similar polarity to a C8 sorbent. Like cyclohexyl, PH exhibits a slightly
polymeric sorbent that can bind both polar and nonpolar analytes. The different selectivity from other nonpolar sorbents. This added selectivity
large particle size makes Nexus ideal for extracting highly viscous samples results from the electron density of the aromatic ring. Retention of planar,
such as horse urine. conjugated organic molecules is enhanced compared to the aliphatic
bonded phases.
PCB SPEC PH offers similar selectivity to Bond Elut PH.

Bond Elut PCB is tailor-made to extract a wide range of polychlorinated
biphenyls (PCBs) from transformer oil and other organic matrices. Bond PPL
Elut PCB is a multiphase cartridge that consists of a charged polar surface
packed in an ultraclean 3 mL polypropylene tube. Using Bond Elut PCB • Sorbent description: functionalized SDVB polymer
and optimized method (M2647), quantitative recoveries of a wide range of • High surface area for excellent retention of polar analytes
PCB compounds have been obtained using a simple one-solvent procedure. • Typical sample types: phenols from waste water
When Bond Elut PCB is used in combination with the CP-Select 28/31 GC
capillary column (Part Number CP7479), a wide range of PCB compounds, Bond Elut PPL, a functionalized styrene-divinylbenzene polymer, has been
including PCB 28 and 31, can be isolated and resolved in half the time of optimized for the extraction of highly polar species from large-volume
other SPE and GC procedures water samples. Featuring a proprietary high-purity spherical polymer
with an extremely nonpolar surface, Bond Elut PPL retains even the most
polar compounds, such as phenols, and achieves high recoveries and fast
Phenylboronic Acid (PBA) extraction speeds.and fast extraction speeds.
• Strong covalent retention mechanism
• Multiple secondary interactions Propylsulfonic Acid (PRS)
• Ideal for cis-diol extraction
• Bonded functional group: propylsulfonic acid
• Primary retention mechanism: strong cation exchange
• Typical sample types: water, biological samples
PBA (Phenylboronic Acid) is a unique sorbent consisting of a phenylboronic

acid bonded to a silica gel surface. The PBA sorbent covalently binds cis-diol,
cis-amino alcohols, and other related functional groups. Because covalent Bond Elut PRS is a strong cation exchange sorbent that is also very polar.
retention involves an interaction of 10-100 times greater energy than As such, PRS exhibits weak nonpolar interactions. The pKa of PRS is low,
other extraction mechanisms, compounds are more tightly bound to the and cationic analytes must be eluted either by high ionic strength, or by
sorbent surface, resulting in superior clean-up. The boronate group has a neutralizing the charge on the cationic analyte. Therefore, PRS is most
high specificity for cis-diol containing compounds such as catechols, nucleic effective for weaker cations such as pyridinium compounds. Also, PRS is
acids, low molecular weight proteins and carbohydrates. PBA is especially recommended where nonpolar secondary actions of SCX interfere with the
effective in the isolation of catecholamines from biological fluids and for extraction.
the separation of RNA from DNA. It may also be applied to cis-amino alco-
hols such as propranolol. Retention is usually strongest when the analytes’
functional groups are coplanar.
PSA Silica (SI)
• Bonded functional group: ethylenediamine-N-propyl (primary and • Sorbent description: native silica, unfunctionalized
secondary amines) • Primary retention mechanism: strongly polar
• Primary retention mechanism: weak anion exchange (aqueous matrix) • Typical sample types: nonpolar organic solvents, oils, and lipids
or polar (nonpolar organic matrix), chelation
• Typical sample types: water, biological fluids, and organic extracts
Silica is generally regarded as the most polar sorbent available. Unbonded,

activated silica is also fairly acidic. SI is one of the best sorbents available
Bond Elut PSA is an anion exchange sorbent that is similar to NH2. for selectively separating compounds of very similar structure. Applying
Preparation
PSA has two amine groups that offer slightly greater ionic capacity (1.4 the analytes in a nonpolar solvent, then gradually increasing the solvent
Sample
meq/g) than NH2 (1.1 meq/g), and the pKas of its primary and secondary polarity in successive wash steps by adding increasing concentrations
amine groups are higher (10.1 and 10.9, respectively). PSA is therefore a of polar modifiers, such as THF or ethyl acetate, can accomplish this
stronger anion exchanger than NH2. The PSA functional group is also a separation.
very good bidentate ligand, making PSA an excellent sorbent for chelation
applications (useful for metal ion extractions). Its higher carbon loading Bond Elut SI and SPEC SI offer similar selectivity.
makes it a less polar sorbent than NH2, and thus a better choice for very
polar compounds that retain too strongly on NH2 sorbent.SPEC PSA offers TCA
similar selectivity to Bond Elut PSA.
• Optimized for tricyclic antidepressant (TCA) extraction
• Broad concentration range (20-2000 ng/mL)
SAX
TCA is a nonpolar sorbent optimized for extraction of tricyclic
• Strongest anion exchanger available
antidepressants (TCAs). Each lot of Bond Elut TCA is specifically quality
• Quaternary ammonium chloride for intermediate selectivity
tested for recovery of TCAs, ensuring reproducibly good performance.
TOP
SAX (Strong Anion eXchange) is the strongest anion exchange sorbent TOP (Trityl-on Oligo Purification) was created with a very high
available. Because its functional group is a quaternary amine, the sorbent selectivity for trityl-on oligonucleotides. It is based on a unique
is always charged. SAX offers minimal nonpolar interactions because functionalized styrene divinylbenzene material that allows direct loading
any effect of the carbon atoms in the functional group is masked by of the basic deprotection solution. This ensures that all the full-length
the amine. Because SAX is such a strong anion exchanger, it is a good oligonucleotide product will bind the first time it is passed through the TOP
sorbent for retention of weaker anions such as carboxylic acids that cartridge. The sample - sorbent interaction is further maximized through
may not retain strongly enough on weaker anion exchange phases. SAX the controlled particle size distribution of the TOP resin. Also, the large
contains a chloride counterion. The user can modify the selectivity of SAX particle size resin permits gravity flow operation, eliminating the cost of
by conditioning with appropriate buffers, exchanging chloride for ions of vacuum manifolds. The result is high, reproducible yields and a simplified,
greater or lesser selectivity. more robot-friendly process.
SCX
• Bonded functional group: benzenesulfonic acid
• Primary retention mechanism: strong cation exchange
• Typical sample types: aqueous, biological fluids and organic reaction
mixtures
Bond Elut SCX is a strong cation exchanger with a very low pKa, and is
similar to PRS as an ion exchange sorbent. The SCX sorbent has a higher
potential for nonpolar interactions than PRS due to the presence of the
benzene ring. This potential for nonpolar interactions should be considered
when the sorbent is used for ion exchange from aqueous samples. This
dual nature is useful for extracting compounds that exhibit both cationic
and nonpolar character. The overall result of the dual character is superior
clean up using only a single sorbent.SPEC SCX offers similar selectivity to
Bond Elut SCX.

12 OMIX Pipette Tips
OMIX® Pipette Tips
• Extract drugs, peptides and proteins
• Purify 10-100 uL samples
• Elute with 0.5-100 uL
• Directly shoot into the LC/MS/MS or spot onto a MALDI plate
Solutions for Microvolume SPE
Monolithic sorbent tip technology for dependable purification and superior results in Drug
Discovery and Proteomix research.
OMIX pipette tips (patent pending) for microextractions reliably purify and enrich femtomole
to micromole levels of small molecule drugs, peptides and proteins prior to MALDI-TOF or LC/
MS. The unique monolithic sorbent technology used in OMIX consistently outperforms other
microvolume techniques by delivering uniform flow and strong analyte-to-surface interactions
for consistent, high recoveries.
OMIX tips in Proteomix research provide:

• Improved Precision: Achieve up to a 8-fold improvement in RSDs with OMIX monolithic technology
• Increased Recoveries: Isolate up to 50% more peptide with OMIX’s superior binding capacity
• Better MASCOT Results: Obtain higher MASCOT scores, percent sequence coverage, and number of peptides matched for more reliable protein identification
• Higher Peak Intensities: Assure correct protein identification with significant signal improvements
• Greater Confidence: Experience dependable tip performance and prevent precious sample loss
OMIX tips in Pharmacokinetics studies provide:

• Freedom to work with limited amounts of sample: 5 to 200 µL rat or mouse plasma
• Greater sensitivity through low elution volumes: less than 50 µL
• Faster sample prep with direct injection into an LC/MS/MS
OMIX tips contain a C18 or a C4 reversed phase sorbent packed into in 10 or 100 µL pipette tips. The C18 phase is
recommended for small molecules and peptides, while the C4 phase is recommended for proteins. The 100 µL volume tips are
ideal for small molecule applications and larger proteolytic digests (greater than 5µL) where very high capacity is required (up
to 80 µg) and elution volumes greater than 5 µL are acceptable. For proteolytic digests less than 10 µL in volume, use either
the standard OMIX C18 tips for intermediate capacity and elution volumes, or use OMIX C18-MB (minibed) tips for ultra-low
elution volumes.
OMIX Pipette Tips

Sorbent Elution
8 /Pk 8 /Pk 96 /Pk 96 /Pk 576 /Pk 576 /Pk
Phase Volume
0.5-2 µL A57003MBSPL A57003MB A57003MBK
C18 2-10 µL A5700310SPL A5700310 A5700310K
5-100 µL A57003100SPL A57003100 A57003100K
0.5-2 µL A57009MBSPL A57009MB A57009MBK
C4 2-10 µL A5700910SPL A5700910 A5700910K
5-100 µL A57009100SPL A57009100 A57009100K
TOP Trityl-on Oligon Purification 13
TOP™ (Trityl-on Oligon Purification)
• High throughput, gravity flow oligonucleotide purification
• Purify 96 oligos in 45 minutes
• Robot compatible for automated, unattended operation
• Generate reproducible yields with gravity flow
• Eliminate multiple loading steps
• Directly load the ammonia or AMA onto the resin
• Save up to 12 hours of evaporation time
TOP meets the demand for simple, cost effective, high throughput purification of trityl-on oligonucleotides. TOP incorporates a unique 96-well plate with
removable tubes, streamlined gravity flow protocol, polymeric resin and optimized binding solution. Varian’s cutting edge BioSolutions technology delivers
superior yields and purity for standard oligos up to 1 umol in scale and up to 50 mer in length.
Preparation
Sample
96-well plates: Up to 200 nmol scale. TOP will purify oligonucleotides up to 200 nmol in scale
using the 96-well plate format and either the 25 or 50 mg bed mass. These tubes are available in the
following packaging options:
Starter Kits: Simplify the ordering process with two prepackaged solutions. The kit includes a
Preassembled 96-well Plate, TOP Buffer Pak, Collection Plate, Disposable Waste Tray, Well Plate Tube
Removal Tool and TOP User Guide. Each kit will purify up to 96 oligos and is recommended for first-
time users.
Preassembled Plates: Complete 96-well plates minimize assembly time and labor.
Well Plate Tubes: Order either 96 or 24 packs of well plate tubes with the reusable base plate to reduce
the overall cost per oligo while maintaining the 96-well footprint. Two additional bed dimensions are
available in the preassembled Plate and the Well Plate Tube options - a 100 mg bed for applications
that require higher capacity and a 150 mg bed for those customers that prefer vacuum.
Cartridges: Up to 1 umol scale. A standard 6 mL cartridge with 300 or 500 mg of TOP material is available for synthesis scales up to 1 umol. This
configuration is the preferred choice for users that do not require high throughput.
TOP buffers: Perfect for reducing solution preparation time and ensuring improved reproducibility. Each case of the quality controlled TOP BufferPak will
purify over 1200 oligos and contains both the Binding Buffer and the TEAA Solution. Individual cases of the each buffer type are also available.
For additional information, visit www.varianinc.com/biosolutions/
Starter Kits
The kit includes a Preassembled 96-well Plate, TOP Buffer Pak, Collection Plate, Disposable Waste Tray, Well Plate Tube Removal Tool and TOP User Guide.
Volume
Item Name 25 mg 50 mg Quantity
(mL)
TOP Starter Kit for 50 nmol Scale 76009025 1.8 1 Kit
TOP Starter Kit for 200 nmol Scale 76009050 1.8 1 Kit
Preassembled 96-well Plates

Max. Synthesis
Volume
Scale 25 mg 50 mg 100 mg 150 mg Quantity
(mL)
(nmol)
50 75619025 1.8 1
200 75619050 7561901C 7561915C 1.8 1

14 TOP Trityl-on Oligon Purification
TOP Well Plate Tubes
Max. Synthesis
Volume
Scale Quantity 25 mg 50 mg 100 mg 150 mg
(mL)
(nmol)
24 /Pk 75819025 1.8
50
96 /Pk 75719025 1.8
24 /Pk 75819050 7581901C 7581915C 1.8
200
96 /Pk 75719050 7571901C 7571915C 1.8
Preassembled 96-well Plates

Max. Synthesis
Volume
Scale 25 mg 50 mg 100 mg 150 mg Quantity
(mL)
(nmol)
50 75619025 1.8 1
200 75619050 7561901C 7561915C 1.8 1
Cartridges
Max. Synthesis
Volume
Scale 300 mg 500 mg Quantity
(mL)
(nmol)
1000 12102300 12102301 6.0 30
TOP Prepackaged Solutions

Item Name Part No. Quantity
TOP BufferPak, 950 ml 1x Binding Solution,
79100000 1 Kit
3x950 ml 0.1M TEAA Solution
TOP 1x Binding Solution, 4x 950 ml 79100001 4 /Pk
TOP 2x Binding Solution, 950 ml 79100003 1 Each
TOP 2x Binding Solution, 4x950 ml 79100004 4 /Pk
TOP 0.1M TEAA Solution, 4x950 ml 79100002 4 /Pk
TOP 2M TEAA Solution, 4x950 ml 79100005 4 /Pk
Accessories
Disposable Waste Tray 5133001 25 /Pk
96-well Plate Sealing Mat 5133005 50 /Pk
Well Plate Tube Removal Tool 12236107 1 Each
TOP Reusable Baseplate 75400001 1 Each
TOP User Guide 391491900 1 Each
96-Well Collection Plate, 2 ml Well Volume WA77015200 25 /Pk
96-Well Collection Plate, 750 ul Well Volume WA77015750 25 /Pk
FOCUS Polar-Enhanced SPE 15
FOCUS™ Polar-Enhanced SPE
• Unique polar-enhanced SPE phase
• Power Rinse™ delivers cleaner extracts
• Reduced ion suppression
• Simple, universal method
FOCUS features a unique polar-enhanced sorbent technology that delivers outstanding recovery
for polar as well as non-polar analytes. Unlike other polymeric sorbents, FOCUS exhibits hydrogen
bond donor capability that significantly increases the retention of basic analytes. This increased
retention results in cleaner extracts and higher recoveries across a broad spectrum of drugs. The
extraction method is simple and eliminates the complex pH adjustments necessary with ion
Preparation
exchange and mixed mode methods. FOCUS is available in 96-well plate and cartridge formats.
Sample
Compare FOCUS to today’s most popular SPE technology
An independent pharmaceutical lab performed a rigorous study to evaluate the performance of FOCUS. The results were then compared to two
popular polymeric SPE products. In the end, FOCUS clearly outperformed the competition with cleaner extracts and better recovery over a broad
spectrum of drugs (below).

16 FOCUS Polar-Enhanced SPE
Cleaner extracts reduce ion suppression
FOCUS’ improved retention for polar analytes allows use of a strong Power Rinse without causing drug loss. Rigorous washes using 10-20% acetonitrile and
methanol can be used to aggressively remove endogenous matrix interferences. For very polar analytes that are held by strictly polar interactions, e.g.
atenolol, washes of up to 100% can be used. FOCUS produces substantially cleaner extracts compared to alternatives, leading to reduced ion suppression.
FOCUS 96-well SPE

Sorbent Mass
Part No. Quantity
(mg)
10 A59660 1 Each
FOCUS Cartridges
Sorbent Mass
1 mL 3 mL 6 mL Quantity
(mg)
10 A5106010 100 /Pk
20 A5306021 100 /Pk
50 A5606050 100 /Pk
SPEC Disc SPE 17
SPEC® Disc SPE SPEC 96-well Plates
• Trouble-free automation • Monolithic silica extraction disc
• Highly reproducible flow and recoveries • Trouble-free automation
• Faster sample processing • Highly reproducible flow and recoveries
• Faster sample processing
Using an advanced disc design, SPEC delivers superior flow characteristics

and trouble-free automation
For a description of these sorbents, see the Sorbent Selection Guide on

pages 5-11.
Preparation
Method Development Kit
Sample
The SPEC Method Development kit provides a range of sorbents to help you
optimize your sample preparation method.
Sorbent Phase 15 mg Quantity
C2, C8, C18, C18AR, CN, MP1, MP3, PH A59630 1
Silica-based Sorbents
Sorbent Phase 15 mg 30 mg Quantity
C18 A59603 A5960330 1
C18AR A59619 1
C2 A59601 1
Using an advanced disc design, SPEC delivers superior flow characteristics
and trouble-free automation. C8 A59602 1
CN A59606 1
Low elution volumes mean that evaporation and reconstitution steps may DAS A596DAS 1
be eliminated, resulting in faster sample processing. By combining low bed DAU A596DAU 1
mass, ultraclean materials, and optimized selectivity, SPEC delivers higher NH2 A59607 1
recoveries free of the matrix interferences that cause ion suppression. Phenyl A59610 1
SPEC is available in 96-well plate, cartridge, and pipette tip formats. For SI A59608 1
information on available chemistries, refer to the Sorbent Selection Guide
on pages 5-11.
Ion Exchange Sorbents
SPEC is available in both cartridge and 96-well formats. Sorbent Phase 15 mg Quantity
SAX A59605 1
SPEC improves flow and eliminates plugging SCX A59604 1
When combined with an integral anticlogging device, SPEC’s unique
monolithic disc technology achieves highly uniform and reproducible flow
even when processing highly particle-laden samples. This results in highly Mixed Mode Sorbents
reproducible recoveries and fast, trouble-free automation. Sorbent Phase 15 mg Quantity
MP1 A59611 1
SPEC 96-Well Plates offer outstanding flow characteristics. When used MP3 A59620 1
with an automated platform, flow across all 96 wells is uniform and highly PSA A59616 1
reproducible. SCX/C8 A59623 1
Lower elution volumes mean higher productivity

SPEC delivers high recoveries at low elution volumes - as low as 100 µL.
This is due to the high surface area of the SPEC monolithic silica disc that
results in a very high extraction efficiency. By reducing elution volumes,
samples can be directly injected on LC/MS without evaporation and
reconstitution while still achieving required LLOQs.

18 SPEC Disc SPE
SPEC® Well Plate Adapters SPEC Discs
The SPEC adapter allows the SPEC 96-well plate to fit properly into the For a description of thse sorbents, see the Sorbent Selection Guide on
collection plates used with Whatman, Waters, Porvair and similar vacuum pages 5-11.
manifolds. The adapter raises the SPEC 96-well plate 1.5 cm above the
surface of the vacuum manifold to provide the clearance these systems SPEC Discs
require. Sorbent Phase 47 mm 90 mm Quantity
Item Name Part No. Quantity C8 A74702 24 /Pk
SPEC 96-well A74819 20 /Pk
A596AD 1 C18AR
Adapter A79019 12 /Pk
SPEC 96-well
A596ADG 1
Adapter Gasket
SPEC Disc Accessories

SPEC SPE Cartridges Accessories
For a description of these sorbents, see the Sorbent Selection Guide on
SPEC Environmental Disc Manifold A712 1 Each
pages 5-11.
SPEC Environmental Disc Holder 47 mm A713 1 Each
SPEC 1 Liter Flask 40/35 mm A714 1 Each
SPEC 3 ml SPE Cartridges
100/pack SPEC Test Tube 25 mm x 250 mm A715 1 Each
Sorbent Phase 15 mg 30 mg
C18 A5320320 A5320330
C18AR A5321920 A5321930
C18AR/MP1 A5322630
C18AR/MP3 A5322530
C2 A5320120 A5320130
C8 A5320220 A5320230
CN A5320620 A5320630
DAS A532DAS
DAU A532DAU
MP1 A5321120 A5321130
MP3 A5322020 A5322030
NH2 A5320720 A5320730
PSA A5321620 A5321630
Phenyl A5321020 A5321030
SAX A5320520 A5320530
SCX A5320420 A5320430
SCX/C8 A5322330
SI A5320820 A5320830
SPEC 10 ml SPE Cartridges

100/pack
Sorbent Phase 35 mg 70 mg
C18 A5020335
C18AR A5021935
C18AR/MP3 A5022570
C2 A5020135
C8 A5020235
CN A5020635
MP1 A5021135 A5021170
MP3 A5022035
NH2 A5020735 A5020770
PSA A5021635
Phenyl A5021035
SAX A5020535
SCX A5020435
SI A5020835
Empore Disk SPE 19
Empore® Disk Cartridges
Preparation
Sample
C18 HD
High Density (12 µm particle size)
Disk Diameter Quantity
1 mL 3 mL 6 mL
(mm) (/Box)
4 12144064 100
7 12144065 50
10 12144066 30
C18 SD
Standard Density (55 µm particle size)
1 mL 3 mL 6 mL 40 mL
(mm) (/Box)
4 12144001 100
7 12144002 50
10 12144003 30
20 12144062 20
C2 HD
1 mL 3 mL 6 mL
(mm) (/Box)
4 12144007 100
7 12144008 50
10 12144009 30
C2 SD
1 mL 3 mL 6 mL
(mm) (/Box)
4 12144070 100
7 12144071 50
10 12144072 30
C8 HD
1 mL 3 mL 6 mL
(mm) (/Box)
4 12144004 100
7 12144005 50
10 12144006 30
C8 SD
1 mL 3 mL 6 mL
(mm) (/Box)
4 12144069 100
7 12144068 50
10 12144067 30

20 Empore Disk SPE
MPC-Mixed Phase Cation HD
1 mL 3 mL 6m
(mm) (/Box)
4 12144040 100
7 12144041 50
10 12144042 30
MPC-Mixed Phase Cation SD

1 mL 3 mL 6 mL
(mm) (/Box)
4 12144073 100
7 12144074 50
10 12144075 30
SDB-RPS
1 mL 3 mL 6 mL
(mm) (/Box)
4 12144055 100
7 12144056 50
10 12144057 30
SDB-XC
1 mL 3 mL 6 mL
(mm) (/Box)
4 12144059 100
7 12144060 50
10 12144061 30
Empore Extraction Disks

Anion Exchange Extraction Disks SDB-XC Extraction Disks
Disk Diameter Quantity Disk Diameter Quantity
Part No. Part No.
(mm) (/Box) (mm) (/Box)
47 12145012 20 47 12145010 20
90 12145013 10 90 12145011 10
Chelating Extraction Disks C8 Extraction Disks

Part No. Part No.
47 12145029 20 47 12145002 20
90 12145030 10 90 12145034 10
Oil & Grease Extraction Disks C18 Extraction Disks

Part No. Part No.
47 12145038 20 47 12145004 20
90 12145039 10 90 12145007 10
SDB-RPS Extraction Disks Carbon

Disk Diameter Quantity Disk Diameter
Part No. Part No. Quantity
(mm) (/Box) (mm)
47 12145026 20 47 12145040 20 /Box
90 12145027 10
Empore Disk SPE 21
Empore Disk Plates Empore® Disk Manifolds
Deep Well Disk Plates The one-, three- or six-station extraction vacuum manifold offers an
2.5 mL capacity 96-well plates. Standard Density, 55 um particle size. efficient extraction apparatus. These manifolds allow multiple sample
Quantity processing and are more stable and compact than a series of single
Sorbent Phase Part No. glass filtration systems. Using the manifold, up to six extractions can be
(Plate)
C18 SD 12143013 1 completed simultaneously, and a single operator can manage multiple
C2 SD 12143015 1 manifolds. The powerful combination of the Empore disk and the six
C8 SD 12143014 1 station manifold allows for up to 12 one-liter drinking water samples to be
extracted per hour, a tremendous improvement over manual liquid/liquid
MPC SD 12143016 1
methods.
The Empore Extraction Manifold accommodates a set of six 47 mm or three
Preparation
Standard Well Disk Plates
1.2 mL capacity 96-well plates. Standard Density, 55 um particle size. 90 mm glassware. Replacement parts may be ordered separately. A vacuum
Sample
pump or supply is required.
Quantity
Sorbent Phase Part No.
(Plate)
C18 SD 12143003 1 Empore Disk Manifolds
C2 SD 12143009 1 Quantity
Item Name Part No.
(Each)
C8 SD 12143006 1
Single Station 47 mm, Disk Vacuum Manifold,
MPC SD 12143012 1 12146023 1
complete system
Single Station 90 mm, Disk Vacuum Manifold,
12146022 1
complete system
Three Station 47 mm, Disk Vacuum Manifold,
Empore Disk Plate Manifold & Accessories complete system
12146021 1
The standard and deep well plates are compatible with the most commonly Six Station 90 mm, Disk Vacuum Manifold,
12146002 1
complete system
used automated liquid handling systems.
Six Station 47 mm, Disk Vacuum Manifold,
12146001 1
complete system
Empore Disk Plate Manifold
Quantity
Item Name Part No.
(Each)
Empore Disk 96-Well Plate Vacuum Manifold,
Complete
12143100 1 Empore Disk Manifold
Replacement Components
Empore Disk Plate Accessories Empore Disk Manifold Replacement Components
Item Name Part No. Quantity Item Name Part No. Quantity
96 Polypropylene Insert Collection Tube Rack 12143104 10 /Box 300 mL Reservoir for Empore 47 mm
12146015 1 Each
Deep Well, 96-Well Collection Plate 12143101 24 /Box Disk Manifold
Empore Sealing Tape Pad #660 12143105 10 /Pk 1000 mL Reservoir for Empore 47 mm
12146016 1 Each
Disk Manifold
Replacement Gasket for Empore Disk Plate
12143106 5 /Pk 1200 mL Reservoir for Empore 90 mm
Manifold 12146013 1 Each
Disk Manifold
U-Bottom 96-Well Collection Plate 12143103 100 /Box
Clamp for 47 mm, Empore Disk Manifold 12146011 1 Each
V-Bottom 96-Well Collection Plate 12143102 60 /Box
Clamp for 90 mm, Empore Disk Manifold 12146012 1 Each
Empore Manifold Valve Assembly 12146007 1 Each
Empore Manifold Valve Stopcocks 12146006 6 /Box
Empore Filter Aid 400 - Glass Beads Empore PTFE Sleeve 12146018 6 /Box
KEL-F Support Screen for Empore 47 mm
Empore Filter Aid 400 consists of 40 micron, nonporous, inert glass beads 12146009 6 /Box
Disk Manifold
which act as a depth filtration material when placed on top of Empore KEL-F Support Screen for Empore 90 mm
Extraction Disks. 12146010 3 /Box
Disk Manifold
Replacement glass for Empore 47 mm
Empore Filter Aid 400 - Glass Beads 12146003 1 Each
Disk Manifold
Quantity Replacement glass for Empore 90 mm
Item Name Part No. 12146004 1 Each
(Each) Disk Manifold
Filter Aid - Empore Glass Beads - 16 oz. 12146005 1 Support Base for Empore 47 mm
12146017 1 Each
Disk Manifold
Support Base for Empore 90 mm
12146014 1 Each
Disk Manifold
Teflon Coated Stainless Steel Support Screen
12146008 6 /Box
for Empore 47 mm Disk Manifold

22 Bond Elut Cartridge SPE
Varian takes Bond Elut quality control to the next level.
Optical scanners installed throughout our automated assembly process inspect each Bond
Elut tube at multiple points. When defects or imperfections in the product are spotted,
the tube is removed from the assembly line. The entire process is in compliance with the
newest ISO 9001:2000 Quality System guidelines. The result is consistently reliable Bond
Elut cartridges time and time again. With the attention we give to maintaining the highest
possible quality standards, it’s no wonder Bond Elut has been the world’s leading SPE brand
for over 20 years.
BOND ELUT®
• The original market-leading packed bed SPE cartridge
• Unparalleled quality, service and support
• 40 different bonded phases
Bond Elut is the original and still the most trusted name in solid phase extraction. Years of use by demanding scientists at top companies worldwide have
thoroughly documented its many applications and proven its properties. To this day, you will find more literature references for Bond Elut than any other
product in the industry.
Over 40 bonded phases are available in a variety of cartridge formats including straight wall, Laboratory Robot Compatible (LRC) and Bond Elut Junior. For
information on available chemistries, refer to the Sorbent Selection Guide on pages 5-11.
The Varian Bond Elut Difference

• Ongoing Research and Development. Varian continually invests in research and technology to improve the production and quality of Bond Elut and
other SPE products.
• World Class Manufacturing. Continual upgrades in materials and equipment help assure that the quality of Bond Elut remains ahead of the competition.
• Stringent Quality Control. Exacting ISO 9001: 2000 compliant manufacturing standards and inspections guarantee the consistent quality of Bond Elut
• Instant Availability. A large inventory of Bond Elut is maintained in locations worldwide to provide rapid response to customer orders.
• Technical Support. A team of expert scientists is available to consult on customer applications and help solve technical issues.
Bond Elut® AccuCAT Bond Elut AccuCAT LRC Cartridges

Sorbent
Bond Elut AccuCAT cartridges are mixed-mode SPE columns consisting of Volume Quantity
Mass 40 µm
(mL) (/Box)
a strong cation exchange and a strong anion exchange sorbent packed (mg)
into one bed. AccuCAT is effective for the extraction of acidic, basic, and 200 10 12282005 60
neutral compounds from urine and other biological samples. AccuCAT is 600 10 12282001 60
particularly effective for catecholamine extraction from biofluids.
For assistance selecting the best sorbent for your application, please see Bond Elut AccuCAT Straight Barrel Cartridges
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and Sorbent
Volume Quantity
use our Applications Online Scanview database. Mass 40 µm
(mL) (/Box)
(mg)
3 12282003 60
200
6 12282004 30
400 6 12282006 30
600 3 12282002 60
Bond Elut Cartridge SPE 23
Bond Elut® Alumina-A Bond Elut® Alumina-B
• Sorbent: alumina, Al2O3, acidic • Sorbent: alumina, Al2O3, basic
• pH: 4.5 • pH: 10.0
• Particle size: 25 µm • Particle size: 25 µm
• Primary retention mechanism: Lewis acid/base, polar, and ion exchange • Primary retention mechanism: Lewis acid/base, polar, and ion exchange
For assistance selecting the best sorbent for your application, please see For assistance selecting the best sorbent for your application, please see
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
use our Applications Online Scanview database. use our Applications Online Scanview database.
Preparation
Sample
Bond Elut Jr. Bond Elut Jr.
Quantity Quantity
Sorbent Mass 25 µm Sorbent Mass 25 µm
(/Box) (/Box)
500 mg 12162047B 100 500 mg 12162048B 100
1g 12166043B 100 1g 12166044B 100
Bond Elut LRC Cartridges Bond Elut LRC Cartridges

Sorbent Sorbent
Volume Quantity Volume Quantity
Mass 25 µm Mass 25 µm
(mL) (/Box) (mL) (/Box)
(mg) (mg)
100 10 12113020 50 100 10 12113021 50
500 10 12113046 50 500 10 12113047 50
Bond Elut Straight Barrel Cartridges Bond Elut Straight Barrel Cartridges
Sorbent Volume Quantity Sorbent Volume Quantity
25 µm 25 µm
Mass (mL) (/Box) Mass (mL) (/Box)
50 mg 1 12102069 100 50 mg 1 12102070 100
100 mg 1 12102021 100 100 mg 1 12102022 100
500 mg 3 12102047 50 500 mg 3 12102048 50
1g 6 12256043 30 1g 6 12256044 30
3g 6 12256062 30

Bond Elut® Alumina-N Bond Elut Atrazine
• Sorbent: alumina, Al2O3, neutral
• pH: 7.5
• Primary retention mechanism: Lewis acid/base, polar, and ion exchange
Atrazine is a widely used herbicide in the United States that has been
Alumina, like silica, is an extremely polar sorbent. The alumina surface tends implicated in serious health problems, including tumor formation and
to be slightly more stable under high pH conditions than unfunctionalized endocrine problems. The biodegradation of atrazine and the harmful affects
silica. The smaller particle size ensures high extraction efficiency and small on the environment by some of its dealkylated metabolites, de-ethylatrazine
(50 mg) sorbent beds are therefore commonly used. and deisopropylatrazine, have placed these herbicide degradation products
in the “watch list” of the National Pollutants Survey (NPA).
This sorbent is prepared with an electrically neutral surface. This favors the
retention of electron-rich compounds like aromatic species and aliphatic The Varian Bond Elut Atrazine cartridge is a specially bonded low-load,
amines. It also favors retention of compounds containing electronegative high-flow C18 phase. The controlled carbon content of the sorbent was
groups (for example, functional groups with oxygen, phosphorus and designed to retain atrazine as well as its polar metabolites by a non-polar/
sulfur atoms). It maybe used in extracting either non-polar or polar species polar retention mechanism. The bigger particle size of the sorbent and the
from aqueous or non-aqueous samples respectively. diameter of the tube allow fast flow for large volume water samples. And,
the large bed mass of the Bond Elut Atrazine phase has been optimized to
For assistance selecting the best sorbent for your application, please see provide enough capacity to ensure good recovery of atrazine and its polar
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and metabolites.
use our Applications Online Scanview database.
With the use of the Bond Elut Atrazine extraction cartridge and optimized
method for surface water (M2638) in combination with a Varian HPLC
Bond Elut Jr.
diode array-detection system, quantitative recoveries of atrazine and
Quantity its dealkylated metabolites have been achieved, even at extremely low
Sorbent Mass 25 µm
(/Box) concentrations of 0.02 ppb. These concentration levels are more than two
500 mg 12162049B 100 orders of magnitude below the tolerance level specified by the EPA (3 ppb)
1g 12166045B 100 and those set by the most stringent European agencies (0.1 mg/L and 0.5
mg/L). The extraction and analysis methods are fast, reproducible and
require minimal organic solvent consumption.
Bond Elut LRC Cartridges
Sorbent Bond Elut Straight Barrel Cartridges
Volume Quantity
Mass 25 µm Sorbent
(mL) (/Box) Volume Quantity
(mg) Mass 120 µm
(mL) (/Box)
100 10 12113022 50 (g)
500 10 12113048 50 3 20 12256111 20
Bond Elut Straight Barrel Cartridges BioBond Elut

Sorbent Volume Quantity
25 µm • Desalt synthetic oligonucleotides
Mass (mL) (/Box)
50 mg 1 12102071 100 • No minimum molecular weight limit
100 mg 1 12102023 100 • Recoveries >98%
500 mg 3 12102049 50 Choose BioBond Elut for fast, simple desalting of synthetic oligonucleotides
10 g 60 12256050 16 from final deprotection reaction mixtures. With BioBond Elut there are no
20 g 60 12256059 16 minimum molecular weight limits, so oligos less than 5,000 Daltons can
be desalted with >98% recoveries. And, while typical desalting media can
leave as much as 2% of the original salt in your sample, BioBond Elut
Bond Elut Cartridges with Stainless Steel Frits provides 100% salt-free samples.
Sorbent
Volume Quantity For assistance selecting the best sorbent for your application, please see
Mass 25 µm
(mL) (/Box) the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
(mg)
100 1 12124023 100 use our Applications Online Scanview database.
500 3 12124046 50
BioBond Elut Straight Barrel Cartridges
Sorbent
Volume Quantity
Mass 40 µm
(mL) (/Box)
(mg)
200 6 12102140 30
500 6 12102141 30
Bond Elut® C1 Bond Elut® C2
• Base silica: 40 µm irregularly-shaped, acid-washed silica, • Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica,
60 Å mean pore size 60 Å mean pore size
• Bonded functional group: methyl • Bonded functional group: ethyl
• Endcapped: yes • Endcapped: yes
• Typical carbon loading: 4.1% • Typical carbon loading: 5.6%
• Primary retention mechanism:very weakly nonpolar • Primary retention mechanism: weakly nonpolar
• Typical sample types: plasma, urine, and aqueous samples • Typical sample types: plasma, urine, and aqueous samples
Preparation
Bond Elut C1 sorbent Bond Elut C2 sorbent
Sample
Due to the single carbon functional group, C1 is the least retentive of all C2 is a fairly polar sorbent because of the short chain length of the
alkyl group bonded phases for nonpolar compounds. However, because the functional group, which exposes the silica surface. C2 is often used as a
sorbent is endcapped, masking the polar silanol activity, easier retention replacement for C18 and C8 when molecules are retained too strongly by
and elution of polar and multifunctional compounds can be achieved. the latter. C2’s polarity is slightly lower than a CN for polar interactions.
It is popular for the extraction of drugs from plasma and serum since,
For assistance selecting the best sorbent for your application, please see if correct wash and elution conditions are selected, flat baselines can be
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and achieved.
For assistance selecting the best sorbent for your application, please see
Bond Elut LRC Cartridges the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
Sorbent use our Applications Online Scanview database.
Volume Quantity
Mass 40 µm
(mL) (/Box)
(mg)
100 10 12113004 50
300 10 12113053 50 Sorbent
Volume Quantity
500 10 12113030 50 Mass 40 µm 120 µm
(mL) (/Box)
(mg)
100 10 12113003 14113003 50
200 10 12113026 14113026 50
Bond Elut Straight Barrel Cartridges
500 10 12113029 14113029 50
Sorbent
Volume Quantity
Mass 40 µm
(mL) (/Box)
(mg)
50 1 12102061 100
1 12102004 100 Sorbent Volume Quantity
100 40 µm 120 µm
3 12102090 50 Mass (mL) (/Box)
500 3 12102031 50 1 12102060 14102060 100
50 mg
3 12105029 50
1 12102003 14102003 100
100 mg
3 12102117 50
Bond Elut Cartridges with Stainless Steel Frits
200 mg 3 12102027 14102027 50
Sorbent 3 12102030 50
Volume Quantity 500 mg
Mass 40 µm 6 12102115 30
(mL) (/Box)
(mg)
1g 6 12256003 14256003 30
100 1 12124004 100
2g 12 12256056 20
500 3 12124028 50
5g 20 12256141 20
10 g 60 12256057 16

Sorbent
Volume Quantity
Mass 40 µm 120 µm
(mL) (/Box)
(mg)
100 1 12124003 14124003 100
500 3 12124027 14124027 50

Bond Elut® C8 Bond Elut® C18
• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, • Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60
60 Å mean pore size Å mean pore size
• Bonded functional group: octyl • Bonded functional group: trifunctional octadecyl
• Primary retention mechanism: moderately nonpolar • Primary retention mechanism: strongly nonpolar
• Typical sample types: water, aqueous biological samples • Typical sample types: water, aqueous biological fluids
Bond Elut C8 sorbent Bond Elut C18 sorbent
C8 is very similar in property to C18, but is not as retentive for nonpolar C18 is the most hydrophobic silica-based sorbent available. It is the most
compounds due to its shorter hydrocarbon chain. C8 is a good replacement popular SPE sorbent because of its extreme retentive nature for nonpolar
for C18 when analytes are too strongly retained on C18 for effective compounds. C18 is generally regarded as the least selective silica based
elution. The potential of C8 for polar interactions with analytes is somewhat sorbent, since it retains most organic analytes from aqueous matrices,
higher than for C18 because the shorter hydrocarbon chain does not often a benefit when the compounds of interest vary widely in structure.
mask the silica surface as effectively, but polar interactions are still not a The potential for polar interactions between analytes and sorbent is
significant property of C8. The C8 sorbent has been successfully utilized in less significant with C18 than with any other sorbent because of the
the simultaneous extraction of fat- and water-soluble vitamins from human predominant effect of the long hydrocarbon chain. When analyzing small
serum. to intermediate molecules, C18 can also be utilized for desalting aqueous
matrices prior to ion exchange because salts pass through it unretained.
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and For assistance selecting the best sorbent for your application, please see
use our Applications Online Scanview database. the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
Bond Elut Jr.
Sorbent Mass Quantity Bond Elut Jr.
40 µm
(mg) (/Box) Quantity
500 100 Sorbent Mass 40 µm
12162029B (/Box)
500 mg 12162028B 100
1g 12166001B 100
Sorbent
Volume Quantity
Mass 40 µm 120 µm
(mg)
(mL) (/Box) Bond Elut LRC Cartridges
100 10 12113002 14113002 50 Sorbent
Volume Quantity
200 10 12113025 14113025 50 Mass 40 µm 120 µm
(mL) (/Box)
500 10 12113028 14113028 50 (mg)
100 10 12113001 14113001 50
200 10 12113024 14113024 50
Bond Elut Straight Barrel Cartridges 500 10 12113027 14113027 50
40 µm 120 µm
Mass (mL) (/Box)
1 12102059 14102059 100 Bond Elut Straight Barrel Cartridges
50 mg
3 12105028 50 Sorbent Volume Quantity
1 12102002 14102002 100 40 µm 120 µm
100 mg Mass (mL) (/Box)
3 12102100 50 1 12102058 14102058 100
200 mg 3 12102026 14102026 50 50 mg
3 12105027 50
3 12102029 14102029 50 1 12102001 14102001 100
500 mg 100 mg
6 12102053 14102053 30 3 12102099 50
1g 6 12256002 14256002 30 1 12102096 100
2g 12 14256016 20 200 mg
3 12102025 14102025 50
5g 20 12256024 14256024 20 3 12102028 14102028 50
10 g 60 12256032 14256032 16 500 mg
6 12102052 14102052 30
3 12102118 50
1g 6 12256001 14256001 30
60 12256060 16
Sorbent 1.5 g 3 12102149 50
Volume Quantity
Mass 40 µm 120 µm 2g 12 14256015 20
(mL) (/Box)
(mg)
5g 20 14256023 20
100 1 12124002 14124002 100
10 g 60 14256031 16
3 12124026 14124026 50 (continued)
500
6 124002 30
Bond Elut C18 Cartridges with Stainless Steel Frits Bond Elut® C18 INT
40 µm 120 µm • Base silica: 40 µm irregularly-shaped, acid-washed silica,
Mass (mL) (/Box)
100 mg 1 12124001 100 500 Å mean pore size
500 mg
3 12124025 14124025 50 • Bonded functional group: trifunctional octadecyl
6 124004 30 • Endcapped: yes
1g 6 12124110 30 • Typical carbon loading: 12.2%
• Primary retention mechanism: strongly nonpolar
• Typical sample types: water, aqueous biological fluids
Bond Elut® C18 EWP

• Base silica: 40 µm irregularly-shaped, acid-washed silica,
500 Å mean pore size
Preparation
• Bonded functional group: trifunctional octadecyl
Sample
Bond Elut C18 sorbent
• Endcapped: yes
• Typical carbon loading: 12.2% C18 INT is very similar in properties to the standard Varian C18 sorbent but
• Primary retention mechanism: strongly nonpolar with a slightly lower carbon loading. This intermediate load bonded C18
• Typical sample types: water, aqueous biological fluids is also thoroughly end capped ensuring rapid release of basic drugs. The
carbon content has been optimized to differentiate between metabolites
of species like prostaglandins. In addition, the narrow pore size helps
exclude large molecules such as proteins.

the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
The C18 EWP sorbent is based upon standard particle size silica but with use our Applications Online Scanview database.
500 Å pores to allow more efficient extraction of macromolecules typical
of most bimolecular separations. The larger pore size of the sorbent enables Bond Elut LRC Cartridges
large molecules (> 15,000 MW), which are typically excluded from standard Sorbent
Volume Quantity
porosity phases, to permeate into the pores and interact with the bonded Mass 40 µm
(mL) (/Box)
silica surface. With C18 EWP, successful separation of complex mixtures of (mg)
proteins, peptides or nucleotides can be achieved. Also, C18 EWP is a good 50 10 12113120 50
sorbent choice for desalting matrices with large molecular weight com- 100 10 12113121 50
pounds of interest prior to LC/MS or LC/MS/MS, capillary electrophoresis or 200 10 12113122 50
any other analytical technique that is adversely affected by the presence of 500 10 12113123 50
salts in the sample. (Note: see BioBond Elut section on optimized bed mass
and application for desalting oligonucleotides.)
For assistance selecting the best sorbent for your application, please see Sorbent Volume Quantity
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and 40 µm
Mass (mL) (/Box)
use our Applications Online Scanview database. 50 mg 1 12102120 100
100 mg 1 12102121 100
Bond Elut LRC Cartridges 200 mg 3 12102122 50
Sorbent 500 mg 3 12102123 50
Volume Quantity
Mass 40 µm 1g 6 12256120 30
(mL) (/Box)
(mg)
50 10 12113068 50
100 10 12113069 50
200 10 12113070 50
500 10 12113071 50

40 µm
Mass (mL) (/Box)
50 mg 1 12102136 100
100 mg 1 12102137 100
500 mg 3 12102139 50
200 mg 3 12102138 50
1g 6 12256130 30

Bond Elut® C18 LO Bond Elut® C18 OH
• Bonded functional group: trifunctional octadecyl • Bonded functional group: octadecyl
• Endcapped: yes • Endcapped: no
• Primary retention mechanism: strongly nonpolar • Primary retention mechanism: moderately nonpolar, plus significant
• Typical sample types: water, aqueous biological fluids polar secondary interactions
• Typical sample types: biological matrices, aqueous samples

C18 LO, like C18 and C18 INT, is a highly retentive nonpolar silica-
based sorbent due to the long hydrocarbon chain. However, the low C18 OH is a nonendcapped version of the octadecyl bonded phases that
carbon loading of C18 LO gives unique selectivities over other octadecyl enables the silanols on the silica surface to be more active. This low-load
sorbents. The percent carbon loading has been optimized for polar C18 has controlled silanol activity under tight QC tolerances for best
analyte extraction from environmental matrices. Quantitative recoveries batch-to-batch reproducibility. The silanol activity permits fractionation
of Atrazine metabolites have been successfully achieved with C18 LO of metabolites and enhances retention of basic compounds compared with
sorbents. (Note: see Bond Elut Atrazine section for optimized bed mass and endcapped C18. In addition, the 150 Å intermediate pore size is good for
application information.) intermediate molecular weight compounds that may not be retained on a
standard 60 Å material.
Sorbent Bond Elut Jr.
Volume Quantity
Mass 40 µm Sorbent
(mL) (/Box) Quantity
(mg) Mass 40 µm
(/Box)
50 10 12113110 50 (mg)
100 10 12113111 50 500 12162046B 100
200 10 12113112 50
500 10 12113113 50
Sorbent
Volume Quantity
Bond Elut Straight Barrel Cartridges Mass 40 µm 120 µm
(mL) (/Box)
(mg)
40 µm 100 10 12113019 14113019 50
Mass (mL) (/Box)
50 mg 1 12102110 100 500 10 12113045 14113045 50
100 mg 1 12102111 100
200 mg 3 12102112 50
500 mg 3 12102113 50
1g 6 12256110 30 40 µm 120 µm
Mass (mL) (/Box)
50 mg 1 12102065 14102065 100
100 mg 1 12102020 14102020 100
500 mg 3 12102046 14102046 50
1g 6 12256040 14256040 30

Sorbent
Volume Quantity
Mass 40 µm 120 µm
(mL) (/Box)
(mg)
100 1 12124020 14124020 100
500 3 12124043 14124043 50
Bond Elut® CBA Bond Elut® Cellulose
• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica,
• Bonded functional group: carboxylic acid
• Sorbent pKa: 4.8
• Mean capacity: 0.35 meq/g
• Endcapped: yes
• Typical percent carbon loading: 7.4%
• Primary retention mechanism: weak cation exchange The Varian Bond Elut Cellulose column utilizes a pure microgranular
• Typical sample types: water, biological fluids cellulose powder that is packed between two 20 µm polyethylene frits in
an ultraclean 3 mL polypropylene extraction column. The unique rod-like
Preparation
shape and controlled particle size of this variety of Cellulose ensure that
it is free-flowing when dry. In addition, the material is manufactured
Sample
from high purity cotton with a very high a-cellulose content that ensures
Bond Elut CBA carboxylic acid sorbent
optimum column packing properties. Thus, high density, more uniform,
smooth-packed column beds are achieved. In addition, the cellulose phase
CBA is a medium polarity sorbent based on carboxy propyl functionalized
is very stable over a wide pH range and contains an extremely low metal
silica, exhibiting either polar or nonpolar interactions depending on the
content (Fe and Cu content less than 5 ppm). The combination of surface
matrix or solvent environment. Its prime characteristic is its weak cation
area and polymeric structure results in a sorbent with excellent capacity.
exchange properties. Because of its pKa value of 4.8, CBA does not require
extreme basic conditions for elution that are required by other cation
The cellulose media contains numerous hydroxyl groups. Because of its
exchange sorbents. Above a pH of 4.8, the majority of functional groups
polar nature, it is able to accept high loading of many polar substances
carry a negative charge that can be used for retaining cationic compounds.
from aqueous and organic phases. The free hydroxyl groups’ binding
Dropping the pH to 2.8 effectively neutralizes CBA, permitting the retained
properties are complemented by the presence of very small quantities of
analytes to elute from the sorbent. For this reason, CBA is often the best
residual aldehydes, ketones and carboxyl groups. These weak groups add
choice for cation exchange, especially when dealing with very strong
reducing and ion-exchange properties.
cations (i.e. high pKa cations). In addition, CBA shows a wider range of
selectivity towards counter-ions than other cation exchange phases,
This microgranular cellulose support has been utilized for large scale
enabling more options for elution.
bioprocessing as well as a prefilter for suspended matter (i.e. cell debris and
suspended fats), as a prefractionation step prior to reverse phase HPLC, for
separation of biomolecules, and as an affinity chromatography matrix.
Bond Elut LRC Cartridges use our Applications Online Scanview database.
Sorbent
Volume Quantity
Mass 40 µm 120 µm Bond Elut Straight Barrel Cartridges
(mL) (/Box)
(mg)
Sorbent
100 10 12113011 14113011 50 Volume Quantity
Mass Part No.
500 10 12113037 14113037 50 (mL) (/Box)
(mg)
300 3 12102095 500

40 µm 120 µm
Mass (mL) (/Box)
50 mg 1 12102073 100
1 12102011 14102011 100
100 mg
3 12102097 50
200 mg 3 12102124 50
500 mg 3 12102038 14102038 50
1g 6 12256009 14256009 30
2g 12 12256058 20

Sorbent
Volume Quantity
Mass 40 µm 120 µm
(mL) (/Box)
(mg)
100 1 12124011 14124011 100
500 3 12124035 14124035 50

Bond Elut Certify® Bond Elut Certify® II
• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, • Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, 60
60 Å mean pore size Å mean pore size
• Bonded functional group: Mixed-mode sorbent bed: octyl (C8) and • Bonded functional group: mixed-mode sorbent bed: octyl and
benzenesulfonic acid (SCX) quaternary amine (SAX)
• Endcapped: No • Endcapped: no
• Typical percent carbon loading: 9.0% • Typical percent carbon loading: 8.6%
• Primary retention mechanism: Bimodal, nonpolar and strong cation • Primary retention mechanism: bimodal, nonpolar and strong anion
exchange exchange
• Typical sample types: Urine, biological fluids • Typical sample types: urine, biological fluids
Bond Elut LRC Cartridges Bond Elut Certify II was developed specifically for the rapid and effective
Sorbent extraction of acidic drugs and metabolites from urine and other biological
Volume Quantity matrices. Certify II is a mixed-mode cartridge packed with nonpolar C8 and
Mass 40 µm 120 µm
(mL) (/Box) strong anion exchange (SAX) sorbent. It has been optimized for extraction
(mg)
130 10 12113050 14113050 50 of acidic drugs such as 11-nor-delta-9-tetrahydrocannabinol-9-carboxylic
200 10 12113054 14113054 50 acid, salicylic acid, ibuprofen, acetaminophen, and other compounds
300 10 12113052 14113052 50 possessing both nonpolar and anionic character.
Bond Elut Straight Barrel Cartridges the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
40 µm 120 µm
Mass (mL) (/Box)
50 mg 3 12105030 50 Bond Elut LRC Cartridges
3 12102051 14102051 50 Sorbent
130 mg Volume Quantity
Mass 40 µm 120 µm
6 12256146 30 (mL) (/Box)
(mg)
3 12102145 50
200 mg 100 10 12113063 50
6 12256145 30
200 10 12113051 14113051 50
3 12102081 50
300 mg
6 12102082 30
500 mg 6 12102093 14102093 30 Bond Elut Straight Barrel Cartridges
1g 6 12102085 14102085 30 Sorbent Volume Quantity
40 µm 120 µm
Mass (mL) (/Box)
50 mg 3 12105031 50
200 mg 3 12102080 14102080 50
6 12102084 30
500 mg
6 14102084 30
1g 6 12102088 14102088 30
Bond Elut® CH (cyclohexyl) Bond Elut® CN-E
• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica, • Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica,
• Bonded functional group: cyclohexyl • Bonded functional group: cyanopropyl
• Primary retention mechanism: moderately nonpolar • Primary retention mechanism: moderately nonpolar (aqueous matrix)
• Typical sample types: water, aqueous samples or polar (nonpolar organic matrix)
• Typical sample types: aqueous (nonpolar), organic (polar)
Preparation
Bond Elut CH cyclohexyl sorbent
Sample
Bond Elut CN-E cyanopropyl sorbent
Cyclohexyl is a medium polarity sorbent that exhibits unique selectivities

for certain analytes. When employed as a nonpolar sorbent, CH has the A medium polarity sorbent with many uses, CN-E is ideal for applications
approximate polarity of a C2 sorbent. The polar subsurface is an important in which extremely nonpolar compounds would irreversibly retain on more
factor among CH properties, as indicated by the ability of CH to retain nonpolar sorbents, such as C18 or C8. This endcapped version of the cyano
polar analytes such as phenol from aqueous matrices. Because of its unique sorbent is best utilized when extracting analytes from an aqueous matrix.
selectivity, CH is often a good choice when other nonpolar sorbents such as
C18, C8, C2, and PH do not provide the desired selectivity. For assistance selecting the best sorbent for your application, please see
For assistance selecting the best sorbent for your application, please see use our Applications Online Scanview database.
use our Applications Online Scanview database. Bond Elut LRC Cartridges
Sorbent
Bond Elut LRC Cartridges Volume Quantity
Mass 40 µm 120 µm
(mL) (/Box)
Sorbent (mg)
Volume Quantity
Mass 40 µm 120 µm 100 10 12113007 14113007 50
(mL) (/Box)
(mg) 500 10 12113033 14113033 50
100 1 12113006 14113006 50
500 3 12113032 14113032 50
Bond Elut Straight Barrel Cartridges 40 µm 120 µm
Mass (mL) (/Box)
Sorbent Volume Quantity 50 mg 1 12102064 14102064 100
40 µm 120 µm
Mass (mL) (/Box) 100 mg 1 12102007 14102007 100
50 mg 1 12102063 14102063 100 500 mg 3 12102034 14102034 50
100 mg 1 12102006 14102006 100 5g 20 12256025 20
500 mg 3 12102033 14102033 50
1g 6 12256005 14256005 30
2g 12 12256039 14256039 20 Bond Elut Cartridges with Stainless Steel Frits
Sorbent
Volume Quantity
Mass 40 µm 120 µm
(mL) (/Box)
Bond Elut Cartridges with Stainless Steel Frits (mg)
Sorbent 100 1 12124007 14124007 100
Volume Quantity
Mass 40 µm 120 µm 500 3 12124031 14124031 50
(mL) (/Box)
(mg)
100 1 12124006 14124006 100
500 3 12124030 14124030 50

Bond Elut® CN-U Bond Elut® DEA
• Bonded functional group: cyanopropyl • Bonded functional group: diethylaminopropyl
• Endcapped: no • Sorbent pKa: 10.7
• Typical percent carbon loading: 7.8% • Mean capacity: 1.0 meq/g
• Primary retention mechanism: polar (nonpolar organic matrix) or weak • Endcapped: no
nonpolar (aqueous matrix) • Typical percent carbon loading: 8.5%
• Typical sample types: nonpolar solvents, organic extracts of aqueous • Primary retention mechanism: weak anion exchange (aqueous matrix)
sample or polar (nonpolar organic matrix)
• Typical sample types: water, biological fluids, nonpolar extracts
Bond Elut CN-U cyanopropyl sorbent

Bond Elut DEA diethylaminopropyl sorbent
Unendcapped CN-U is a medium polarity sorbent. It is less retentive than
Si or 2OH for very polar analytes that might be retained irreversibly on Bond Elut DEA bears some resemblance to Bond Elut NH2 in its properties,
these more polar sorbents. This Unendcapped cyano phase is ideally suited as does Bond Elut PSA. DEA has slightly lower capacity as an anion
for the extraction of polar compounds from a nonpolar matrix such as exchange sorbent (1.0 meq/g) than NH2, and a more nonpolar character
hexane or oils. due to the additional carbon chain on the functional group. The carbon
chains make DEA a medium polarity sorbent, even with the presence of
For assistance selecting the best sorbent for your application, please see the main functionality. DEA is somewhat more polar than C8, but less polar
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and than C2 or CN.
Bond Elut LRC Cartridges the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
Volume Quantity
Mass 40 µm 120 µm
(mL) (/Box)
(mg) Bond Elut LRC Cartridges
100 10 12113008 14113008 50
Sorbent
500 10 12113034 14113034 50 Volume Quantity
Mass 40 µm 120 µm
(mL) (/Box)
(mg)
100 10 12113016 14113016 50
Bond Elut Straight Barrel Cartridges 500 10 12113042 14113042 50
40 µm 120 µm
Mass (mL) (/Box)
50 mg 1 12102066 14102066 100 Bond Elut Straight Barrel Cartridges
100 mg 1 12102008 14102008 100 Sorbent
200 mg 3 50 Volume Quantity
12102135 Mass 40 µm 120 µm
(mL) (/Box)
500 mg 3 12102035 14102035 50 (mg)
1g 6 12256006 14256006 30 50 1 12102078 14102078 100
2g 12 12256017 14256017 20 100 1 12102016 14102016 100
5g 20 14256025 20 500 3 12102043 14102043 50
10 g 60 12256033 14256033 16

Bond Elut Cartridges with Stainless Steel Frits Sorbent
Volume Quantity
Sorbent Mass 40 µm 120 µm
Volume Quantity (mL) (/Box)
Mass 40 µm 120 µm (mg)
(mL) (/Box) 100 1 12124016 14124016 100
(mg)
100 1 12124008 14124008 100 500 3 12124040 14124040 50
500 3 12124032 14124032 50
Bond Elut® ENV Bond Elut® Diol (2OH)
• Sorbent: polymeric wide-pore styrene-divinylbenzene (SDVB) polymer • Base silica: 40 µm irregularly-shaped, acid-washed silica,
• Applications for explosive residues 60 Å mean pore size
• Excellent for polar biomolecules • Bonded functional group: diol
• Used for sulfated or glucuronide-bound steroids • Endcapped: no
• High flow-through: 1 liter in less than 20 minutes • Typical percent carbon loading: 6.8%
• Efficient elutions • Primary retention mechanism: polar
• Typical sample types: nonpolar organic extracts, oils, and lipids
Bond Elut ENV, a high purity styrene-divinylbenzene (SDVB) polymer,
has been optimized for the extraction of polar organic residues, such as
herbicide metabolites and explosives, from large volume water samples.
Bond Elut ENV features a 125 µm highly cross-linked spherical polymer
Preparation
that can process one liter water samples in a fraction of the time
Sample
Bond Elut diol sorbent
required by other sample preparation methods. The absence of secondary
interactions on the polymer surface makes it easier to elute a wide range
of molecular species with just one elution solvent. Although primarily used For assistance selecting the best sorbent for your application, please see
for environmental applications, Bond Elut ENV can also be used for clinical the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
applications including the extraction of highly polar metabolites, such as use our Applications Online Scanview database.
glucuronide-bound molecules from human fluids.
Bond Elut 2OH LRC Cartridges
For assistance selecting the best sorbent for your application, please see Sorbent
Volume Quantity
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and Mass 40 µm
(mL) (/Box)
use our Applications Online Scanview database. (mg)
100 10 12113009 50
Bond Elut Straight Barrel Cartridges 500 10 12113035 50
125 µm
Mass (mL) (/Box)
25 mg 1 100
Bond Elut 2OH Straight Barrel Cartridges
12105011
50 mg 1 12105012 100 Sorbent Volume Quantity
40 µm
Mass (mL) (/Box)
60 mg 3 12105017 50
50 mg 1 12102067 100
1 12105013 100
100 mg 100 mg 1 12102009 100
3 12105014 50
500 mg 3 12102036 50
3 12105015 50
200 mg 1g 6 12256007 30
6 12255014 30
3 12105016 50
500 mg
6 12255011 30
1g 6 12255012 30 EnvirElut® Pesticide, Herbicide &
PAH Cartridges
The extreme purity and high capacity of the EnvirElut sorbents make
them especially suitable for the extraction of a wide range of compounds
from aqueous matrices. Every lot of EnvirElut is rigorously tested for
retention of environmental pollutants from water samples before it is
approved for sale. The EnvirElut sorbent is then packed into the straight
barrel Bond Elut cartridge configuration most suitable for each specific
environmental analysis. EnvirElut Herbicides, PAH, Pesticides, and Phenols
use conventional SPE cartridges that are processed using conventional Vac
Elut vacuum manifolds, and they are not compatible with the EnvirElut
1664 manifold system.

Volume Sorbent Quantity
Sorbent Phase Part No.
(mL) Mass (/Box)
HERBICIDE 3 500 mg 12272006 50
PAH 6 1g 12272005 30
PESTICIDE 6 500 mg 12272004 30
PHENOLS 20 5g 12272002 20

Bond Elut® Florisil Bond Elut® LMS
• Sorbent: Florisil - MgO3Si • Sorbent: ultraclean, highly cross-linked styrene-divinylbenzene (SDVB)
• Particle size: 200 µm polymer
• Primary retention mechanism: polar • Optimized 75 µm particle size for easy throughput of biological
• Typical sample types: environmental, organic extracts samples such as urine, plasma, or serum
• High, controlled surface area for consistent extraction efficiency
Florisil is a magnesia-loaded silica gel. Like silica, it is extremely polar • High capacity allows larger sample loads or a smaller sorbent bed,
in nature and ideal for the isolation of polar compounds from nonpolar resulting in better analyte concentration
matrices. The larger particle size of the Florisil material enables fast flow • Removes proteins efficiently
of large volume samples and thus is a potential alternative to silica when • Worry-free use for SPE and GC/MS and LC/MS applications
using viscous samples. Florisil is utilized for the separation of chlorinated
pesticides and is used in numerous AOAC- and EPA-regulated methods. Varian’s Bond Elut LMS polymeric sorbent lets you elute samples without
having to add amine modifiers, buffers, or acids. The elimination of
For assistance selecting the best sorbent for your application, please see secondary interactions means that elution of analytes can be achieved
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and with pure organic solvents or HPLC mobile phase compatible solvent
use our Applications Online Scanview database. mixtures of low ionic strength. These characteristics are crucial in
ensuring compatibility with LC/MS or other sensitive analytical techniques.
Bond Elut Jr. Featuring optimized particle size and a large surface area for extraction
Sorbent Quantity efficiency, Bond Elut LMS provides interference-free extractions. Producing
200 µm excellent recovery and reproducibility, the LMS sorbent will ensure worry-
Mass (/Box)
500 mg 12162050B 100 free use and reduce costly downtime.
1g 12166014B 100
Bond Elut LRC Cartridges use our Applications Online Scanview database.
Sorbent
Volume Quantity Bond Elut LRC Cartridges
Mass 200 µm
(mL) (/Box)
(mg) Sorbent
Volume Quantity
100 10 12113023 50 Mass 75 µm
(mL) (/Box)
500 10 50 (mg)
12113049
50 10 12115022 50
100 10 12115023 50
200 µm Bond Elut Straight Barrel Cartridges
Mass (mL) (/Box)
50 mg 1 12102072 100 Sorbent Volume Quantity
75 µm
100 mg 1 100 Mass (mL) (/Box)
12102024
25 mg 1 12105021 100
500 mg 3 12102050 50
50 mg 1 12105022 100
3 12102109 50
1 12105023 100
1g 6 12256014 30 100 mg
3 12105024 50
20 12256047 20
200 mg 3 12105025 50
12 12256022 20
2g 3 12105026 50
20 12256046 20 500 mg
6 12255021 30
5g 20 12256030 20
1g 6 12255022 30
10 g 60 12256038 16

200 µm
Mass (mL) (/Box)
100 mg 1 12124024 100
500 mg 3 12124047 50
3 12126013 50
1g
6 124007 30
Bond Elut® NH2 Bond Elut Jr.
Sorbent Quantity
• Base silica: 40 µm or 120 µm irregularly shaped, acid-washed silica, 40 µm
Mass (/Box)
60 Å mean pore size 500 mg 12162041B 100
• Bonded functional group: aminopropyl 1g 12166012B 100
• Sorbent pKa: 9.8
• Mean capacity: 1.10 meq/g Bond Elut LRC Cartridges
• Endcapped: no
Sorbent
• Typical carbon loading: 6.7% Volume Quantity
Mass 40 µm 120 µm
• Primary retention mechanism: weak anion exchange (aqueous matrix) (mL) (/Box)
(mg)
or polar (nonpolar organic matrix) 100 10 12113014 14113014 50
200 10 12113067 50
500 10 12113040 14113040 50
Preparation
Sample
Bond Elut NH2 aminopropyl sorbent
40 µm 120 µm
Mass (mL) (/Box)
NH2, a very polar sorbent, can utilize both hydrogen bonding and anion 50 mg 1 100
12102076 14102076
exchange. Since the pKa of the NH2 sorbent is 9.8, at any pH below 9.8, the 100 mg 1 12102014 14102014 100
majority of the functional groups are positively charged. NH2 is a weaker 3 12102089 50
anion exchanger than sorbents like SAX (a quaternary amine sorbent that 200 mg
6 12102106 30
is always charged) and is therefore a better sorbent choice for retention of 300 mg 3 12102108 50
very strong anions, such as sulfonic acids, which may be retained irrevers- 3 12102041 14102041 50
500 mg
ibly on SAX. Because an ethyl group supports the NH2 functionality, it can 6 12256045 30
be used for nonpolar isolations from polar samples, but its strong polarity 3 12102107 50
1g
is its primary characteristic. Like 2OH and SI, NH2 is excellent for separa- 6 12256012 14256012 30
tion of structural isomers. 2g 12 14256020 20
5g 20 14256028 20
10 g 60 14256036 16
use our Applications Online Scanview database. Bond Elut Cartridges with Stainless Steel Frits
Sorbent
Volume Quantity
Mass 40 µm 120 µm
(mL) (/Box)
(mg)
100 1 12124014 14124014 100
500 3 12124038 14124038 50
Bond Elut® PBA

• Base silica: 40 µm irregularly-shaped, acid-washed silica, fluids, and for the separation of RNA from DNA. Also, it may be applied to
60 Å mean pore size cis-amino alcohols such as propanol. Retention is usually strongest when
• Bonded functional group: phenylboronic acid the analytes’ functional groups are coplanar.
• Endcapped: no
• Typical percent carbon loading: 7.9% For assistance selecting the best sorbent for your application, please see
• Primary retention mechanism: covalent bond with analyte possessing the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
vicinal diol groups use our Applications Online Scanview database.
• Typical sample types: aqueous, biological fluids
Sorbent
Volume Quantity
Mass 40 µm
(mL) (/Box)
(mg)
100 10 12113018 50
Bond Elut PBA phenylboronic acid sorbent
PBA is a unique sorbent consisting of a phenylboronic acid covalently Bond Elut Straight Barrel Cartridges
linked to a silica gel surface. Because covalent retention involves an Sorbent
Volume Quantity
interaction with 10-100 times greater energy than other extraction Mass 40 µm
(mL) (/Box)
mechanisms, compounds are more tightly bound to the sorbent surface (mg)
resulting in a more complete cleanup. The boronate group has a high 1 12102018 20
100
1 12102019 100
specificity for cis-diol-containing compounds like catechols, nucleic acids,
500 6 12102105 30
low molecular weight proteins and carbohydrates. PBA has proven to be
especially effective in the isolation of catecholamines from biological

Bond Elut® PCB Bond Elut® PPL
• Sorbent: unique functionalized styrene-divinylbenzene (SDVB) polymer
• Perfect for phenols in waste water
• High surface area for extreme retention of polar analytes
• High flow properties, even for dirty water
Bond Elut PPL, a styrene-divinylbenzene (SDVB) polymer, has been

Using the Bond Elut PCB cartridge with an optimized method (M2647), optimized for the extraction of highly polar species from large volume
quantitative recoveries of a wide range of PCB compounds have been water samples. Featuring a high purity spherical polymer which has a
obtained using a simple procedure that uses only one solvent. When the proprietary, derivitized, nonpolar surface, Bond Elut PPL retains even
Bond Elut PCB SPE column is used in combination with the CP-Select 28/31 the most polar classes of compounds, such as phenol, and achieves clean
capillary column (Part Number CP7479), a wide range of PCB compounds, extracts, high recovery levels, and fast extraction speeds. Its extreme
including PCB 28 and 31, can be isolated and resolved in half the time of hydrophobicity and surface area of 600 m2/g ensures reproducibility,
other SPE and GC procedures. quantitative retention, and the highest possible recoveries.
For assistance selecting the best sorbent for your application, please see For assistance selecting the best sorbent for your application, please see
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
use our Applications Online Scanview database. use our Applications Online Scanview database.
Sorbent 125 µm
Volume Quantity Mass (mL) (/Box)
Mass 40 µm
(mL) (/Box) 25 mg 1 12105001 100
(g)
50 mg 1 12105002 100
1 3 12105032 50 1 12105003 100
100 mg
3 12105004 50
200 mg 3 12105005 50
3 12105006 50
500 mg
6 12255001 30
3 12102148 50
1g
6 12255002 30
Bond Elut® PH
• Base silica: 40 µm or 120 µm irregularly-shaped, acid-washed silica,
• Bonded functional group: phenyl
• Endcapped: yes
• Typical carbon loading: 10.7%
• Primary retention mechanism: moderately nonpolar
• Typical sample types: water, biological fluids
Bond Elut PH phenyl sorbent
Our Bond Elut PH phenyl sorbent is most commonly employed for nonpolar
extractions, with a similar polarity to a C8 sorbent. Like cyclohexyl, PH
exhibits a slightly different selectivity from other nonpolar sorbents. This Bond Elut PH Straight Barrel Cartridges
added selectivity results from the electron density of the aromatic ring. Sorbent Volume
40 µm 120 µm
Quantity
Retention of planar, conjugated organic molecules is enhanced compared Mass (mL) (/Box)
to the aliphatic bonded phases. 50 mg 1 12102062 14102062 100
100 mg 1 12102005 14102005 100
For assistance selecting the best sorbent for your application, please see 500 mg 3 12102032 14102032 50
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and 1g 6 12256004 14256004 30
Bond Elut PH LRC Cartridges Bond Elut PH Cartridges with Stainless Steel Frits
Sorbent Sorbent
Volume Quantity Volume Quantity
Mass 40 µm 120 µm Mass 40 µm 120 µm
(mL) (/Box) (mL) (/Box)
(mg) (mg)
100 10 12113005 14113005 50 100 1 12124005 14124005 100
500 10 12113031 14113031 50 500 3 12124029 14124029 50
Bond Elut® PRS Bond Elut® PSA
• Bonded functional group: propylsulfonic acid • Bonded functional group: ethylenediamine-N-propyl
• Sorbent pKa: <1.0 • Sorbent pKa: 10.1 and 10.9
• Mean capacity: 0.18 meq/g • Mean capacity: 1.40 meq/g
• Endcapped: no • Endcapped: no
• Primary retention mechanism: strong cation exchange • Primary retention mechanism: weak anion exchange (aqueous matrix),
• Typical sample types: water, biological samples or polar (nonpolar organic matrix), or chelation
• Typical sample types: water, biological fluids, and organic extracts
Preparation
Sample
Bond Elut PRS propylsulfonic acid sorbent
Bond Elut PSA ethylenediamine-N-propyl sorbent

A strong cation exchange sorbent that is also very polar, PRS does not exhibit
any appreciable degree of nonpolar interactions. In nonpolar solvents, PRS is
PSA is similar to NH2. An anion exchange sorbent, PSA has two amine
capable of polar and hydrogen bonding interactions. As the pKa of PRS is low,
groups that offer higher ionic capacity than NH2 (1.4 meq/g vs. NH2’s 1.1
cationic compounds must usually be eluted either by a high ionic strength
meq/g), and the pKas of its primary and secondary amine groups are higher
eluant, or by neutralizing the charge on the cationic isolate. Therefore, PRS is
(10.1 and 10.9, respectively). PSA is therefore a stronger anion exchanger
most effective for weaker cations such as pyridinium compounds. The rela-
than NH2. The PSA functional group is a very good bidentate ligand,
tive lack of nonpolar secondary interactions, combined with its low capacity,
making PSA an excellent sorbent for chelation. Its higher carbon content
gives PRS a unique set of selectivity properties.
makes it a more nonpolar sorbent than NH2 and thus a better choice for
very polar compounds that retain too strongly on NH2 sorbent.
Sorbent Bond Elut Jr.
Volume Quantity
Mass 40 µm Sorbent Quantity
(mL) (/Box) 40 µm
(mg) Mass (/Box)
100 10 12113012 50 500 mg 12162042B 100
500 10 12113038 50 1g 12166050B 100
Bond Elut Straight Barrel Cartridges Bond Elut LRC Cartridges

Sorbent Volume Quantity Sorbent
40 µm Volume Quantity
Mass (mL) (/Box) Mass 40 µm 120 µm
(mL) (/Box)
(mg)
50 mg 1 12102074 100
100 10 12113015 14113015 50
100 mg 1 12102012 100
500 10 12113041 14113041 50
200 mg 3 12102094 50
500 mg 3 12102039 50
1g 6 12256010 30 Bond Elut Straight Barrel Cartridges
40 µm 120 µm
Mass (mL) (/Box)
Bond Elut Cartridges with Stainless Steel Frits 50 mg 1 12102077 14102077 100
Sorbent 100 mg 1 12102015 14102015 100
Volume Quantity
Mass 40 µm 500 mg 3 12102042 14102042 50
(mL) (/Box)
(mg)
1g 6 12256140 30
100 1 12124012 100
2g 12 12256055 20
500 3 12124036 50
3g 6 12105044 30
10 g 60 12256041 16

Sorbent
Volume Quantity
Mass 40 µm 120 µm
(mL) (/Box)
(mg)
100 1 12124015 14124015 100
500 3 12124039 14124039 50

Bond Elut® SAX Bond Elut® SCX
• Bonded functional group: trimethylaminopropyl • Bonded functional group: benzenesulfonic acid
• Sorbent pKa: quaternary amine, always charged • Sorbent pKa: <1.0
• Mean capacity: 0.85 meq/g • Mean capacity: 0.79 meq/g
• Endcapped: no • Endcapped: no
• Primary retention mechanism: strong anion exchange • Primary retention mechanism: strong cation exchange
• Typical sample types: aqueous, biological samples • Typical sample types: aqueous, biological fluids
Bond Elut SCX benzenesulfonic acid sorbent

Bond Elut SAX trimethylaminopropyl sorbent
SAX is the strongest anion exchange sorbent available. Because its functional SCX is a strong cation exchanger with a very low pKa, similar to PRS. However
group is a quaternary amine, the sorbent is always charged. SAX offers the SCX sorbent has a higher potential for nonpolar interactions than PRS,
minimal nonpolar interactions because any effect of the carbon atoms in due to the presence of the benzene ring on its surface. This nonpolar char-
the functional group is masked by the amine. Because SAX is such a strong acter of SCX should be considered when the sorbent is used for ion exchange
anion exchanger, it is a good sorbent choice for retention of weaker anions from aqueous solvent systems. This dual nature is useful with compounds that
such as carboxylic acids that may not retain strongly enough on weaker anion exhibit both cationic and nonpolar character. The overall result of the dual
exchange phases. It is supplied in the chloride counter-ion form. The user may character is superior clean up using only a single sorbent.
modify its selectivity by conditioning it with appropriate buffers, as described
in The Handbook of Sorbent Extraction Technology (P/N 12245005). For assistance selecting the best sorbent for your application, please see the
Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and use
For assistance selecting the best sorbent for your application, please see the our Applications Online Scanview database.
Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and use
our Applications Online Scanview database. Bond Elut Jr.
Quantity
Bond Elut Jr. Sorbent Mass 40 µm
(/Box)
Quantity 500 mg 12162040B 100
Sorbent Mass 40 µm
(/Box) 1g 12166011B 100
500 mg 12162044B 100
1g 12166013B 100
Sorbent
Volume Quantity Mass 40 µm 120 µm
Mass 40 µm 120 µm (mL) (/Box)
(mL) (/Box) (mg)
(mg) 100 10 12113013 14113013 50
100 10 12113017 14113017 50 500 10 12113039 14113039 50
500 10 12113043 14113043 50
40 µm 120 µm Sorbent Volume Quantity
Mass (mL) (/Box) 40 µm 120 µm
Mass (mL) (/Box)
50 mg 1 12102079 14102079 100
50 mg 1 12102075 14102075 100
1 12102017 14102017 100
100 mg 1 12102013 14102013 100
3 12102125 50 100 mg
3 12102098 14102040 50
3 12102044 14102044 50
500 mg 500 mg 3 12102040 50
6 12102144 30
1g 6 12256011 14256011 30
3 12102087 50
1g 1.5 g 3 12102104 50
6 12256013 14256013 30
6 12256053 30
1.5 g 3 12102103 50 2g
12 14256019 20
6 12256051 30
2g 3g 6 12256054 30
12 12256021 14256021 20
5g 20 14256027 20
3g 6 12256052 30
10 g 60 14256035 16
5g 20 12256029 14256029 20
10 g 60 12256037 14256037 16
Sorbent
Volume Quantity Mass 40 µm 120 µm
Mass 40 µm 120 µm (mL) (/Box)
(mL) (/Box) (mg)
(mg)
100 1 12124013 14124013 100
100 1 12124017 14124017 100
500 3 12124037 14124037 50
500 3 12124041 14124041 50
Bond Elut® SI Bond Elut® TCA
• Base silica: 40 µm or 120 µm irregularly-shaped silica, • Fast, low-cost tricyclic antidepressants analysis
60 Å mean pore size • Pretested to give average recoveries >90%
• Bonded functional group: native silica, unfunctionalized
• Endcapped: no Each lot of Bond Elut TCA material is manufactured to exacting standards
• Typical percent carbon loading: 0% and tested specifically for drug recovery. Both a straight barrel and a LRC
• Primary retention mechanism: strongly polar Bond Elut configuration are available, packed with 100 mg of the Bond
• Typical sample types: nonpolar organic solvents, oils, and lipids Elut TCA sorbent.
Preparation
Sample
Bond Elut SI silica sorbent Bond Elut LRC Cartridges
Sorbent
Silica is generally regarded as the most polar sorbent available. Unbonded, Volume Quantity
Mass 40 µm
activated silica is also fairly acidic. SI is one of the best sorbents available (mL) (/Box)
(mg)
for selectively separating compounds of very similar structure. Applying 100 10 12113062 50
the analytes in a nonpolar solvent, then increasing the solvent polarity by
adding increasing concentrations of polar modifiers, such as THF or ethyl-
acetate, at successive steps can accomplish this separation. Bond Elut Straight Barrel Cartridges
Sorbent
For assistance selecting the best sorbent for your application, please see Volume Quantity
Mass 40 µm
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and (mL) (/Box)
(mg)
use our Applications Online Scanview database. 100 3 12102146 50
Bond Elut Jr.

Sorbent Quantity
40 µm
Mass (/Box) Bond Elut® Layered Phase Cartridges
500 mg 12162037B 100
Three PSA and SAX layered columns are available which can be utilized in
1g 12166008B 100
the USFDA Luke method for multiresidue pesticide analysis. Two straight-
walled Bond Elut versions are available containing 250 mg of each phase
and 500 mg of each phase. In addition, the newly redesigned Bond Elut
Jr. cartridge enables the PSA/ SAX combination, 500 mg per phase, to be
Sorbent packed into a single cartridge.
Volume Quantity
Mass 40 µm 120 µm
(mL) (/Box)
(mg)
100 10 12113010 14113010 50
500 10 12113036 14113036 50 use our Applications Online Scanview database.

Quantity
Sorbent Volume Quantity Item Name Part No.
40 µm 120 µm (/Box)
Mass (mL) (/Box) AL-N III/SI-HF, 1g of each phase in a 6 mL
50 mg 1 12102068 14102068 100 12256063 30
Bond Elut cartridge
100 mg 1 12102010 14102010 100 FL/C18, 2g of each phase in a 20 mL Bond
12256142 20
200 mg 20 12102147 20 Elut cartridge
SAX/PSA, 250 mg of each phase in a 3 mL
500 mg 3 12102037 14102037 50 12102116 30
Bond Elut cartridge
1g 6 12256008 14256008 30 SAX/PSA, 500 mg of each phase in a 6 mL
12256061 30
1.5 g 3 12102119 50 Bond Elut cartridge
2g 12 14256018 20 Si/CN-E, 500 mg of each phase in a 6 mL
12256066 30
Bond Elut cartridge
5g 20 14256026 20
10 g 60 14256034 16
Bond Elut Jr.
Quantity
Bond Elut Cartridges with Stainless Steel Frits Item Name Part No.
(/Pk)
Sorbent SAX/PSA, 500 mg of each phase in a Bond
Volume Quantity 12166052B 100
Mass 40 µm 120 µm Elut Jr. cartridge
(mL) (/Box)
(mg)
100 1 12124010 14124010 100
500 3 14124034 50

ASPEC Capped SPE Bond Elut® Cartridges ASPEC Caps
• ASPEC-compatible Bond Elut cartridges • Convert Bond Elut 1, 3 and 6 ml cartridges for use in Gilson SPE systems.
• Eleven popular Bond Elut sorbents
• Three cartridge sizes These special caps produce a positive pressure seal with the needle in the
• Convenient and cost-effective Gilson ASPEC™, ASPEC XL and ASPEC XL4 solid phase extraction systems,
and allow the positive pressure applied by the Gilson instruments to
Varian’s standard 1, 3 and 6 mL Bond Elut cartridges are fitted with special aspirate the sample and solvents through Bond Elut SPE cartridges.
caps that produce a positive pressure seal with the needle in the Gilson
ASPEC™, ASPEC XL and ASPEC XL4 solid phase extraction systems. The Also, we sell a variety of Bond Elut cartridges with these caps preinstalled,
addition of the ASPEC-compatible caps allows the positive pressure applied ready for use. See the Bond Elut Cartridge SPE section.
by the Gilson instruments to aspirate the sample and solvents through the
Bond Elut SPE cartridges. Varian supplies the cartridges precapped from
ASPEC Caps for SPE Cartridges
our factory, and in large package quantities to provide a cost-effective
ready-made solution. Also, the caps and standard Bond Elut columns may Quantity
Item Name Part No.
(/Pk)
be purchased separately. See the Bond Elut Accessories section.
Gilson Adapter 1 mL, Yellow 12131034 1000
For assistance selecting the best sorbent for your application, please see Gilson Adapter 3 mL, Blue 12131035 1000
Gilson Adapter 6 mL, Red 12131036 1000
ASPEC Capped SPE Bond Elut Cartridges

Sorbent
Sorbent Quantity
Mass 1 mL 3 mL 6 mL
Phase (/Pk)
(mg)
50 22102058 500
100 22102001 500
C18
200 22102025 500
500 22102028 22102052 500
50 22102060 500
100 22102003 500
C2
200 22102027 500
500 22102030 500
50 22102059 500
100 22102002 500
C8
200 22102026 500
500 22102029 22102053 500
50 22102073 500
CBA 100 22102011 500
500 22102038 500
50 22102066 500
CN-E 100 22102008 500
500 22102035 500
130 22102051 500
Certify
300 22102081 22102082 500
Certify II 200 22102080 500
50 22102076 500
NH2 100 22102014 500
500 22102041 500
50 22102079 500
SAX 100 22102017 500
500 22102044 500
50 22102075 500
SCX 100 22102013 500
500 22102040 500
50 22102068 500
Silica 100 22102010 500
500 22102037 500
NEXUS Nonpolar Application Development Kits
• Unique, ultra-clean copolymeric material For more convenient and
• No conditioning needed for hydrophobic analytes economical method de-
• High flow for viscous samples velopment, Varian offers
• Ideal for veterinary sciences and environmental applications Bond Elut Application De-
velopment kits for sorbent
Nexus provides simple, clean SPE on a unique polymeric sorbent. It offers screening. Three types of
a nonpolar retention mechanism with no conditioning required, enabling Application Development
increased throughput or improved ease of field sampling in environmental kits are available: nonpolar,
applications. polar/ion exchange, and
Nexus delivers reproducibly high recoveries. This performance is achieved from polymer/silica C18. An instruction manual outlining fundamental extraction
the use of a proprietary, highly cross-linked, spherical polymeric sorbent that considerations is included with each kit.
Preparation
can bind both polar and non-polar analytes. The large particle size makes
Sample
Nexus ideal for extracting highly viscous samples such as horse urine. The nonpolar Application Development Kit contains these Bond Elut silica-
based sorbents: C18, C8, C2, CH, PH, and CN-U. The 100 mg kit includes 10
For assistance selecting the best sorbent for your application, please see cartridges of each sorbent. The 500 mg kit includes 5 cartridges of each
the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and sorbent.
NEXUS LRC Cartridges the Sorbent Selection Guide on pages 5-11, or visit www.varianinc.com and
Volume Quantity
Mass Part No.
(mL) (/Box)
(mg) Nonpolar Application Development Kits
30 10 12113100 50 C18, C8, C2, CH, PH, and CN-U Bond Elut cartridges, 10 each
60 10 12113101 50 Sorbent
Volume Quantity
Mass Part No.
(mL) (/Pk)
(mg)
100 1 12102054 60
NEXUS Straight Barrel Cartridges
500 3 12102055 30
Sorbent
Volume Quantity
Mass Part No.
(mL) (/Box)
(mg)
30 1 12103100 100 Polar/ Ion Exchange Application
60 3 12103101 100
6 12103102 30
Development Kits
200
12 12253101 20
12 12253102 20 The Polar/Ion Exchange
500
20 12253103 20 Application Development
Kit contains these Bond
Elut silica-based sorbents:
CBA, NH2, 2OH, SAX, SCX,
and SI. The 100 mg kit
includes 10 cartridges of
each sorbent. The 500 mg
kit includes 5 cartridges of
each sorbent. A useful instruction manual outlining fundamental extrac-
tion considerations is included with each kit.
Ion Exchange Application Development Kit

SI, NH2, 2OH, SCX, SAX, and CBA Bond Elut cartridges, 5 or 10 each
Sorbent
Volume Quantity
Mass Part No.
(mL) (/Pk)
(mg)
100 1 12102056 60
500 3 12102057 30

Polymer and C18 Varieties Application Sodium Sulfate Drying Cartridges
Development Kit Simplify your sodium sulfate drying step by using cartridges prepacked
In response to the popularity of high-throughput screening, Varian of- with ACS grade, granular anhydrous sodium sulfate. Both the Bond Elut
fers a polymer and C18 method development kit that contains our wide LRC and the Bond Elut Jr. configurations are available. Bond Elut Jr.
range of C18 silicas and nonpolar resins. In addition, both the standard cartridges have Luer fittings top and bottom for easy parallel processing in
(40 µm) particle size and a high flow (120 µm) C18 phase have been conjunction with standard SPE cartridges. Bond Elut LRC cartridges have
included. This kit will allow labs seeking to validate screening methods on a large reservoir above the drying bed and are suitable for use on any
nonpolar sorbents to find the best sorbent option. Each phase is packed standard SPE vacuum manifold.
into 1 mL straight barrel Bond Elut cartridges, which are compatible with
robotic systems. Standard 20 µm, polyethylene frits are used. The 100 mg Sodium Sulfate Drying Cartridges
bed masses are optimized for smaller samples and elution volumes. The Sorbent
Column Volume Quantity
sorbents included in this kit are: Mass Part No.
Format (mL) (/Pk)
(g)
C18 - Our most widely used nonpolar sorbent: New applications are 1 LRC 10 12131033 100
continually being developed on Bond Elut C18. 1.4 Bond Elut Jr. N/A 12162052B 100
2.2 Bond Elut Jr. N/A 12162054B 100
C18 HF -This 120 µm high flow sorbent is effective for viscous or 3 Bond Elut Jr. N/A 12162051B 100
particulate-laden samples, but also offers the added selectivity of silanols 15 Straight Barrel 60 12132004 16
on the silica substrate.
C18 EWP - Extra wide pore size (500 Å) allows for better retention of
macromolecules, proteins, and other large molecules typically excluded
from standard 60 Å pores.
C18 OH - Monofunctional bonding provides slightly lower carbon loading,

enhanced polar characteristics, and greater access to silanols, resulting in a
unique selectivity for nonpolar analytes.
PPL - A functionalized polystyrene-divinylbenzene polymer (PSDVB), PPL

is our most nonpolar sorbent. It is effective in extracting the most polar
organic compounds from aqueous matrices.
ENV - The larger particle size of this pure PSDVB resin makes ENV
useful for particulate-laden samples. In addition, it is useful for applica-
tions requiring extremes of pH.
Polymer and C18 Varieties Application Development Kit

C18, C18 EWP, C18 OH, C18 HF, PPL and ENV Bond Elut cartridges, 10 each
Sorbent
Volume Quantity
Mass Part No.
(mL) (/Pk)
(mg)
100 1 12102154 60
Bond Elut Accessories 43
Empty SPE Cartridges
• Available in sizes from 1 to 60 ml
• Made with clean polypropylene for clean extracts
• Uniform batch-to-batch size for consistent performance
• Economical
A variety of empty reservoirs are available to increase the sample capacity of Bond Elut extraction cartridges.
Also, empty reservoirs may also be purchased with frits and used to pack custom SPE cartridges with bulk
Bondesil sorbents or other customer-specified packing.
Empty SPE Cartridges

Order frits separately, or order our cartridges with preinstalled frits.
Preparation
Volume 1 mL 3 mL 6 mL 12 mL 20 mL 60 mL
Sample
Part No. Price 12131007 12131008 12131009 12131010 12131011 12131012
Quantity 100 /Pk 100 /Pk 100 /Pk 100 /Pk 100 /Pk 100 /Pk
Empty SPE Cartridges with 2 Frits

• Available in sizes from 1 to 60 ml
• 2 polypropylene frits preinstalled
• Made with clean polypropylene for clean extracts
These extra-clean polypropylene reservoirs contain 2 polyethylene frits preinserted into the tubes. This is
an ideal configuration for simple filtration. For custom sorbent packing, additional frits can be purchased
separately.
Empty SPE Cartridges with 2 Frits

Volume 1 mL 3 mL 6 mL 12 mL 20 mL 60 mL
Part No. Price 12131013 12131014 12131015 12131016 12131017 12131018
Frits for SPE Cartridges

• 20 µm polyethylene frits for SPE cartridges
• Made with clean polyethylene for clean extracts
Clean 20 µm polyethylene frits are precut to fit into Bond Elut reservoirs for either filtration or custom packing needs.
Frits for SPE Cartridges

Column
1 ml 3 ml 6 ml 12 ml 20 ml 60 ml
Format
Part No. Price 12131019 12131020 12131021 12131022 12131023 12131024
Diameter 1/4 in. 3/8 in. 1/2 in. 5/8 in. 13/16 in. 1-1/16 in.

44 Bond Elut Accessories
Bond Elut® Adapters Luer Stopcocks
• Connect SPE cartridges in series • Luer stopcocks for SPE cartridges
• Expand cartridge volume • Control flow rates of individual cartridges
• Transfer large-volume samples to any SPE cartridge
• Add versatility to your SPE processing Luer stopcocks are used to provide independent flow control of each
individual Bond Elut cartridge when used with vacuum manifolds. They
are made of solvent-resistant polypropylene, are reusable, and are readily
cleaned.
Luer Stopcocks for SPE Cartridges

Quantity
Description Part No.
(/Pk)
Luer Stopcocks 12131005 15
ASPEC Caps
• Convert Bond Elut 1, 3 and 6 ml cartridges for use in Gilson SPE systems.
These special caps produce a positive pressure seal with the needle in the
Gilson ASPEC™, ASPEC XL and ASPEC XL4 solid phase extraction systems,
and allow the positive pressure applied by the Gilson instruments to
aspirate the sample and solvents through Bond Elut SPE cartridges.
Also, we sell a variety of Bond Elut cartridges with these caps preinstalled,
ready for use. See the Bond Elut Cartridge SPE section.
ASPEC Caps for SPE Cartridges

Quantity
Item Name Part No.
(/Pk)
When a sample volume exceeds the capacity of a Bond Elut cartridge Gilson Adapter 1 mL, Yellow 12131034 1000
reservoir, you can increase the volume capacity by attaching a larger Gilson Adapter 3 mL, Blue 12131035 1000
reservoir to the top of the smaller cartridge using a Bond Elut adapter. Bond Gilson Adapter 6 mL, Red 12131036 1000
Elut adapters fit on top of any Bond Elut cartridge and contain a female Luer
fitting that accommodates the tip of another cartridge. This allows you to:
• Stack cartridges for custom multi-sorbent methods (#1 in the photo).

• Increase any cartridge’s volume capacity by stacking an empty reservoir
on top of the cartridge (#2, 3).
• Use a standard Luer-tipped syringe to apply positive pressure for
processing samples (#4). Luer-tipped syringes fit into any Bond Elut
adapter. Gentle pressure is all that is needed to apply conditioning
solvents, samples, rinse solutions, and elution solvents.
• Pull large-volume samples through any SPE cartridge (#5). Simply
insert one end of a 1/8” OD tube through the opening in the Bond Elut
adapter (forming a seal), and insert the other end of the tube into the
sample container. Then apply a vacuum to the SPE cartridge to pull the
entire sample through.
To select the appropriate adapter, choose the adapter specified for the size
of the lower Bond Elut cartridge. The adapter fits snugly into the top of the
cartridge reservoir. All Bond Elut cartridges and empty SPE cartridges have
a standard Luer tip that is usable with these adapters.
Bond Elut Cartridge Adapters

Quantity
(/Pk)
Adapter caps for 1, 3 and 6 mL Bond Elut
12131001 15
cartridges
Adapter caps for 12 mL & 20 mL Bond Elut
12131003 10
cartridges, and LRC cartridges
Adapter caps for 60 mL Bond Elut cartridges 12131004 10
Envir Elut 1664 45
EnvirElut® 1664 EnvirElut® 1664 Manifolds
• The best available system for Oil and Grease analysis
• Developed for EPA Method 1664
• Each lot pretested using EPA protocol
• Extra low level blanks
• High-flow configuration
• One-of-a-kind, totally enclosed extraction system
Varian’s EnvirElut1664 Oil & Grease solid phase extraction processing

system is ideal for monitoring waste water, aqueous effluent, and
surface/ground water. It is faster, more efficient, and uses less solvent
than sample preparation performed with liquid/liquid extraction. EnvirElut
Preparation
1664 cartridges consistently pass 1 liter dirty water samples in less than 10 A one- and a six-station manifold system are available to accommodate
Sample
minutes without loss of analyte, delivering greater than 90% recovery with the number of samples you need to process. Each manifold system comes
oil and grease standards from 5 mg/L to 1 g/L. Our cartridge design has complete with necessary tubing, vials,
been optimized for maximum flow of large-volume samples, and the use and sampling container. In addition to
of depth filters ensures that even particulate-laden samples are processed the replacement components, Varian
without clogging. The procedure is entirely Freon-free, and offers a solid offers an easy pour, open top, 1 L glass
phase extraction alternative with results equivalent to EPA 1664/ASTM sample reservoir that can be used in place
Method 413.1. EnvirElut 1664 cartridges and the EnvirElut 1664 manifold of the standard glass sample bottle and
were specifically designed to work together, and the 1664 cartridges are 89 mm cap. This reservoir ensures that a
not compatible with Vac Elut or other manifolds. full 1 L sample can easily be loaded and
processed, and is compatible with both
EnvirElut 1664 Cartridges systems.
Quantity
Item Name Part No.
(/Box)
EnvirElut 1664 Manifolds
EPA 1664 Oil & Grease Cartridges with
12272020 20 Quantity
AquaSep Cartridges Item Name Part No.
(Each)
Single Station EnvirElut Manifold,
12235101 1
Complete System
Six Station EnvirElut Manifold,
12235102 1
Complete System
EnvirElut Manifold Replacement Parts

Quantity
Item Name Part No.
(Each)
1 Liter Glass Sample Reservoir 12235157 1
50 mL Collection Tube 12235154 1
89 mm, Adapter Cap for Sample Bottle 12235151 1
89 mm, Glass Sample Bottle 12235153 1
EnvirElut Manifold Viton O-ring 12235155 1
Glass Collection Chamber 12235152 1
Two Place Elution Station Replacement 12235156 1

46 Bond Elut 96
Bond Elut® 96
• Available with many of our most popular Bond Elut sorbents
• Fast revalidation of cartridge to 96-well SPE methods
• Low-profile, automation-friendly 96-well design
The same trusted Varian silica-based sorbents used in our standard Bond Elut cartridge products are now available in the streamlined new Bond Elut 96
Well Plates. Choose Bond Elut 96 for fast revalidation of existing cartridge-based methods to a 96-well format. Bond Elut 96 components are specially
formulated to offer superior cleanliness, flow reproducibility, and reliability. And because we manufacture in-house, fast delivery times are standard.
Varian, Inc. also offers complementary 96-well SPE products, SPEC and Focus. SPEC 96-well SPE plates provide the low elution volumes and superior
extraction efficiency characteristic of monolithic disc-based SPE. Focus 96-well SPE plates offer a unique polar-enhanced SPE phase ideal for preparing
biofluid samples for LC/MS analysis with minimal ion suppression and overall cleaner extracts.
Bond Elut 96 Plates with Silica-based Sorbents

Particle
Quantity
Sorbent Phase 25 mg 50 mg 100 mg Size
(Each)
(µm)
C18 A4960150 A496011C 40 1
C18/OH A496291C 40 1
C2 A4961125 A4961150 A496111C 40 1
C8 A4960325 A4960350 A496031C 40 1
CBA A4960625 A4960650 A496061C 40 1
CH A4962225 A4962250 A496221C 40 1
CN-E A4960425 A4960450 A496041C 40 1
CN-U A4961425 A4961450 A496141C 40 1
Certify A4960925 A4960950 A496091C 40 1
NH2 A4960525 A4960550 A496051C 40 1
PBA A496121C 40 1
PH A496151C 40 1
PSA A4962825 A4962850 A496281C 40 1
SCX A4960725 A4960750 A496071C 40 1
Bond Elut 96 Plates with Polymeric Sorbents

Particle
Quantity
Sorbent Phase 10 mg 30 mg Size
(Each)
(µm)
LMS A4961010 75 1
Nexus A4962030 70 1
Did you know you can convert your straight-

barrel Bond Elut application to 96-well format
with Bond Elut 96?
VersaPlate 96-well SPE 47
VersaPlate® Well Plate Tubes
• Available in a variety of sorbents, bed masses, and particle sizes
• Customize your own 96-well plate
• Eliminate needless and costly waste
• Streamline your SPE method development process
Varian can take you to the next generation of increased lab productivity, targeted at improving method development and optimization in a 96-well
plate configuration. Method development in single block well plates can be costly because screening a variety of sorbents or optimizing bed mass can be
accomplished only with expensive, custom-ordered plates, or by wasteful use of standard plates.
Surveys show that on average 30% of the wells of an SPE plate are unused during the method development process.
Preparation
Varian has solved this problem with its innovative VersaPlate system. VersaPlate allows you to:
Sample
- customize your own 96-well plate
- eliminate needless and costly waste
- streamline your SPE method development process
Individual cartridges snap into a 96-well configuration, allowing you to create a multi-sorbent plate quickly and
easily. Multiple sorbents can then be rapidly screened for effectiveness in an assay. Or, different bed masses of the
same sorbent can be evaluated for maximum capacity and minimum elution volume.
For even greater flexibility, the individual VersaPlate tubes are designed to fit our standard Vac Elut SPE vacuum manifold. This allows you to perform
manual extractions with the confidence that you can scale up to high-throughput automation with no additional method modification. And when your
method is optimized, you can purchase preassembled plates for maximum convenience.
For technical information on the available chemistries, refer to the Sorbent Selection Guide on pages 5-11.
Order the VersaPlate base plate separately.
VersaPlate Tubes - Silica Sorbents

96/pack
Particle
Sorbent Phase Size 25 mg 50 mg 100 mg
(µm)
40 75501025 75501050 7550101C
C18
120 75502050
C18HF 120 75502025 7550201C
C2 40 75511025 75511050 7551101C
C8 40 75503025 75503050 7550301C
CBA 40 75506025 75506050 7550601C
CN-E 40 75504025 75504050 7550401C
CN-U 40 75514025 75514050 7551401C
Certify 40 75509025 75509050 7550901C
Certify II 40 75518025 75518050 7551801C
DEA 40 75517025 75517050 7551701C
NH2 40 75505025 75505050 7550501C
PBA 40 7551201C
PH 40 75515025 75515050 7551501C
SAX 40 75508025 75508050 7550801C
SCX 40 75507025 75507050 7550701C
SI 40 75513025 75513050 7551301C
VersaPlate Tubes - Polymeric Sorbents

96/pack
Sorbent Phase 10 mg 25 mg 30 mg 50 mg 60 mg
LMS 75510010 75510025 75510050
NEXUS 75520010 75520030 75520060
VersaPlate Tubes - Hydromatrix

Purify organic reaction mixtures, or perform bioanalytical liquid/liquid extractions using Varian’s Hydromatrix brand diatomaceous earth, now available in the VersaPlate
format. The 96-well format with 260 mg of Hydromatrix per well allows for extraction of up to 500 uL of biofluid samples, or purification of 96 parallel reactions of up to
2 mL in volume. 96/pack
Sorbent Phase 260 mg
Hydromatrix 75530260

48 VersaPlate 96-well SPE
VersaPlate® Base Plate VersaPlate Preassembled Plates with Hydromatrix and-
Polymer Sorbents
• Customize your own 96-well plate Particle Sorbent
• Eliminate needless and costly waste Sorbent Phase Size Mass Part No.
• Streamline your SPE method development process (µm) (mg)
Hydromatrix 260 75430260
The VersaPlate 96-well base plate accepts VersaPlate cartridges in any 75 10 75410010
configuration you need, allowing you to create a multi-sorbent plate LMS 75 25 75410025
quickly and easily. Multiple sorbents can then be rapidly screened for
75 50 75410050
effectiveness in an assay. Or, different bed masses of the same sorbent can
70 10 75420010
be evaluated for maximum capacity and minimum elution volume.
NEXUS 70 30 75420030
Order VersaPlate tubes separately. 70 60 75420060
VersaPlate Base Plate

Quantity
(Each)
VersaPlate® Manifolds & Accessories
VersaPlate Base Plate only 75400000 1 Keep a supply of replacement parts on hand so your VersaPlate system is
always ready to use.
Description Part No. Quantity
VersaPlate® Preassembled Plates VersaPlate manifold, complete,
12236101 1 Each
acrylic/acetal
• Modular 96-well plate design VersaPlate manifold top, clear acrylic 12236102 1 Each
• Reduces operating costs VersaPlate manifold bottom,
12236103 1 Each
• Streamlines the SPE method development process white acetal
VersaPlate manifold complete shim set 12236104 1 Each
Individual VersaPlate cartridges VersaPlate manifold bottom gasket 12236105 1 Each
snap in to a special 96-well VersaPlate manifold top gasket 12236106 1 Each
base plate that gives you the VersaPlate 8-well sealing strip 12236108 240 /Pk
power to customize your 96-
well plate SPE. Develop new
methods faster by building
your own multisorbent plate
to rapidly screen different
sorbents and bed masses.
Reduce needless and costly
waste when processing small
sample volumes by configuring a plate with the exact number of cartridges
you need, or process large numbers of samples effortlessly by purchasing
preassembled plates.
For technical information on the available chemistries, refer to the Sorbent

Selection Guide on pages 5-11.
VersaPlate Preassembled Plates with Silica-based Sorbents

Particle
Sorbent Phase Size 25 mg 50 mg 100 mg
(µm)
C18 40 75401025 75401050 7540101C
C18HF 120 75402025 75402050 7540201C
C2 40 75411025 75411050 7541101C
C8 40 75403025 75403050 7540301C
CBA 40 75406025 75406050 7540601C
CN-E 40 75404025 75404050 7540401C
CN-U 40 75414025 75414050 7541401C
Certify 40 75409025 75409050 7540901C
Certify II 40 75418025 75418050 7541801C
DEA 40 75417025 75417050 7541701C
NH2 40 75405025 75405050 7540501C
PBA 40 7541201C
PH 40 75415025 75415050 7541501C
SAX 40 75408025 75408050 7540801C
SCX 40 75407025 75407050 7540701C
SI 40 75413025 75413050 7541301C
Bond Elut Matrix 96-Well SPE 49
Bond Elut® Matrix 96-Well Plates
• One-piece design
• Square wells
For assistance selecting the best sorbent for your application, please see the Sorbent Selection Guide on pages 5-11, or visit
www.varianinc.com and use our Applications Online Scanview database.
Sold 1 plate each.
Bond Elut Matrix 96-Well Plates - Silica Sorbents
Preparation
Particle Size
Sorbent Phase 10 mg 15 mg 25 mg 50 mg 100 mg
(µm)
Sample
40 5801000 5801001 5801002 5801003 5801004
C18
120 5802000 5802001 5802002 5802003
C18 OH 40 5801040 5801041 5801042 5801043 5801044
C2 40 5801020 5801021 5801022 5801023 5801024
40 5801010 5801011 5801012 5801013 5801014
C8
120 5802010 5802011 5802012 5802013
CBA 40 5801080 5801081 5801082 5801083 5801084
CN-E 40 5801030 5801031 5801032 5801033 5801034
CN-U 40 5801050 5801051 5801052 5801053 5801054
40 5801110 5801111 5801112 5801113 5801114
Certify
120 5802110 5802111 5802112 5802113
40 5801120 5801121 5801122 5801123 5801124
Certify II
120 5802120 5802121 5802122 5802123
NH2 40 5801060 5801061 5801062 5801063 5801064
Phenyl 40 65401144 65401145 65401146 65401147 65401148
SAX 40 5801100 5801101 5801102 5801103 5801104
SCX 40 5801090 5801091 5801092 5801093 5801094
Silica 40 5801070 5801071 5801072 5801073 5801074
Bond Elut Matrix 96-Well Plates - Polymeric Sorbents

Particle Size
Sorbent Phase 10 mg 15 mg 25 mg 30 mg 50 mg 60 mg
(µm)
LMS 75 5801130 5801131 5801132 5801133
NEXUS 70 65401150 65401151 65401152 65401153

50
Bond Elut® Matrix Collection Plates Bond Elut® Matrix Manifolds & Accessories
The Bond Elut Matrix manifold system also has a range of collection plates The Bond Elut Matrix system’s lightweight acrylic vacuum manifold
and manifold accessories. Collection plates in 0.35, 1 and 2 mL for the features a transparent top for easy viewing of sample and solvent
square well configuration as well as 1 and 2 mL round well collections application steps. The one-piece integrated design offers leak-free flow
plates are available and compatible with both manifold types. Varian can and virtually eliminates cross-contamination. A deep-well anodized
provide sealing caps for the square and round well collection plates. aluminum manifold is also available which offers a more durable, rugged
and solvent resistant format.
Empore Sealing Tape is also compatible with the 96 well plates and
manifold system (see Empore Disk Plate section). 96-Well Plate Manifold Replacement Components
Bond Elut Matrix Collection Plates Drain Cap - 25/box 5133006 25 /Box
Shape Volume Part No. Quantity Item Name Profile Gasket for Acrylic Manifold 5133101 1 Each
Round 96- Well Collection Flat Gasket for Acrylic Manifold 5133102 1 Each
1 mL 5132101 50 /Box
Plate, 1 mL - 50/box
Round Gasket for Aluminum Manifold 5231101 1 Each
Empty 96-Well Plate with
2 mL 5801505 1 Each
Frits
Square 96- Well Collection
350 µL 5133007 50 /Box 96-Well Plate Manifolds
Plate, 350 µL - 50/box
Square 96- Well Collection Quantity
Square 1 mL 5133008 50 /Box Item Name Part No.
Plate, 1 mL - 50/box (Each)
Square 96- Well Collection
2 mL 5133009 50 /Box 96 Well Acrylic Manifold 5133000 1
Plate, 2 mL - 50/box
Aluminum Manifold with the Deep
5231002 1
Well Collection Plate
Sealing Caps for Round Well Plates
Quantity
Shape Part No. Item Name
(/Box)
96-Well Plate Round Well
Round 5132200 50
Sealing Caps - 50/box
Bond Elut Matrix Protein

Precipitation Plate
Protein Precipitation
Quantity
Part No.
(Plate)
65932003 1
Prospekt Online SPE 51
Prospekt Cartridges Prospekt Cartridges
Quantity
Sorbent Phase 1 mm 2 mm
(/Box)
2OH 12221010 100
C18 12221024 12221001 100
C18 OH 12221031 12221008 100
C2 12221026 100
C8 12221025 12221002 100
CBA 12221039 12221016 100
CH 12221029 12221006 100
CN-E 12221030 12221007 100
CN-U 12221032 12221009 100
Certify II 12221102 100
Preparation
DEA 12221021 100
Sample
EnvirElut 12221103 100
Prospekt cartridges are available from Varian packed with the Focus 12221301 96
complete range of Bondesil bonded silica and polymer based NEXUS 12221302 96
sorbents, known worldwide for their superior performance and highly NH2 12221042 12221019 100
reproducible chemistries. Varian offers the 2 mm Prospekt cartridges for PH 12221028 12221005 100
all of the standard phases, and the new 1 mm format for a select number PLRP-S 12221201 100
of sorbents. The 2 mm cartridges offer the benefits of higher capacity and PRS 12221017 100
higher flow rates. The 1 mm configuration offers sharper band resolution PSA 12221020 100
due to less dead volume and lower elution volumes, making them ideal for SAX 12221022 100
direct MS applications. SCX 12221018 100
Prospekt Method Development Kits

Quantity
Item Name Sorbent Phase 2 mm
(/Box)
Prospekt Anion Exchange Application Development Kit #1 Certify II, SAX, PSA, DEA, NH2, 20 each, 20/phase 12222005 100
Prospekt Anion Exchange Application Development Kit #2 SAX, DEA, PRS, CBA, 25 each, 25/phase 12222007 100
Prospekt Cation Exchange Application Development Kit SCX, PRS, CBA, Certify, 25 each, 25/phase 12222004 100
Prospekt Nonpolar Application Development Kit #1 C18, C8, C18-OH, EnvirElut, 25 each, 25/phase 12222001 100
Prospekt Nonpolar Application Development Kit #2 PH, CH, C2, CN-E, 25 each, 25/phase 12222002 100
Prospekt Nonpolar Application Development Kit #3 C18, C8, C2, CN-E, 25 each, 25/phase 12222006 100
Prospekt Hardware
A Prospekt Rapide filling tool is the newest hardware addition. This unit
allows the user to snap cartridges into the Prospekt cartridge strips fast
and easily. Simply drop the cartridges into the hollow handle, clip the
handle plate on, insert the cartridge strip and twist the handle.
Prospekt Hardware
Quantity
(Each)
Prospekt Rapide Filling Tool 12223001 1

52 Captiva Filtration Products
Captiva® 96-well Filter Kits Captiva® 96-well Filter Plates
• Faster than centrifugation • 10 µm and 20 µm filtration plates for plasma and other particle-laden
• Easily automated samples
• No plugging or interference • 0.2 µm and 0.45 µm filtration plates for precipitated proteins and other
• No loss of analytes or nonspecific binding samples
• Excellent for sample storage • Clean and clear filtrates
• Avoid fibrinogen clogging forever • High analyte recovery
• Everything you need to start or replenish your Captiva filtration systems • No nonspecific adsorption
• Perfect for automated systems
Captiva’s unique dual depth filtra- • For use with Duo Seal 96 96-well seals
tion media provides both complete
removal of precipitated proteins and
outstanding resistance to sample
clogging. All Captiva components are
ultraclean, and rigorously tested to
ensure against nonspecific binding.
With Captiva, your plasma samples
are processed quickly and reliably.
Starter kits contain everything you
need. Replacement kits include
everything you need to replenish your Captiva system, but do not include
the CaptiVac vacuum collar.
Captiva 96-well Starter Filter Kits

Each kit contains:
1 CaptiVac Vacuum Collar
5 Captiva Filter Plates
10 Duo Seal 96 96-well plate seals Captiva 96-well filter plates are offered in several versions.
5 Captiva 96-Deep Well 1 mL Collection Plates
5 Captiva collection plate pierceable covers
The Captiva 0.2 µm and 0.45 µm depth filter plates are ideal to prepare
Pore Size Quantity precipitated protein samples for LC/MS analysis. Filtration is simple,
Filter Material Part No.
(µm) (Kit)
versatile, and necessary to prevent clogging of expensive HPLC columns.
0.2 Polypropylene A5960002SK 1
PVDF & Polypropylene A5967045SK 1 The Captiva 10 µm and 20 µm glass fiber filter plates are designed for
0.45
Polypropylene A5960045SK 1 clarifying highly particle-laden samples, such as freshly thawed plasma,
0.6 Polypropylene A5960006SK 1 preventing sample transfer problems due to pipette tip clogging.
10 Glass Fiber A596401000SK 1
20 Polypropylene A596002000SK 1 Captiva 96-well Filter Plates
PVDF = polyvinyldifluoride Pore Size
Filter Material Part No. Quantity
(µm)
0.2 Polypropylene A5960002 5 /Pk
Captiva Replacement Kits PVDF & Polypropylene A5967045 5 /Pk
0.45
Each kit contains: Polypropylene A5960045 5 /Pk
5 Captiva Filter Plates 10 Glass Fiber A596401000 5 /Pk
10 Duo Seal 96 96-well plate seals
Polypropylene A596002000 5 /Pk
5 Captiva 96-Deep Well 1 mL Collection Plates 20
5 Captiva collection plate pierceable covers Polypropylene A596002000B 100 /Pk
PVDF = polyvinyldifluoride
Pore Size
Filter Material Part No. Quantity
(µm)
0.2 Polypropylene A5960002K 1 Kit
PVDF & Polypropylene A5967045K 1 Kit
0.45
Polypropylene A5960045K 1 Kit
10 Glass Fiber A596401000K 1 Kit
20 Polypropylene A596002000K 1 Kit
Captiva Filtration Products 53
Captiva® Filter Cartridges Captiva® 96-well Collection Plates & Cover
Captiva filter cartridges bring all of the benefits of Captiva filtration to • 1 mL 96-deep well collection plates for Captiva filtration and SPEC SPE
the standard SPE cartridge format. The 0.2 µm and 0.45 µm depth filter applications
cartridges are ideal to prepare precipitated protein samples for LC/MS • Pierceable 96-well silicone collection plate covers
analysis. The Captiva 10 µm glass fiber filter cartridge is designed for
clarifying highly particle-laden samples, such as freshly thawed plasma, Captiva 96-deep well collection plates are specially designed for use with
preventing sample transfer problems due to pipette tip clogging. Captiva filtration plates and SPEC SPE solid phase extraction 96-well
plates. The 1 mL capacity provides the volume needed to collect all of your
Captiva Filter Cartridges filtrate or eluate.
Pore Size Volume
Filter Material Part No. Quantity Captiva Pierceable 96-well silicone covers are easily applied to completely
(µm) (mL)
0.2 PVDF & Polypropylene 3 100 /Pk seal our collection plates. This ensures no sample loss due to spillage or
Preparation
A5300002
PVDF & Polypropylene
evaporation, and no sample contamination. The pierceable silicone is
Sample
0.45 3 A5307045 100 /Pk
specially designed for 96-well auto injectors providing easy piercing and
10 Glass Fiber 10 A500401000 100 /Pk
removal with no “coring”.
PVDF = polyvinyldifluoride
Captiva 96-well Collection Plates & Cover

CaptiVac™ Vacuum Collars Captiva 96-Deep well Collection Plate A696001000 10
Captiva 96-Deep well Collection Plate for
A696001000H 10
• For use with Captiva Filtration and 96-Deep Well collection plates, and Hamilton MICROLAB ATplus 2
SPEC SPE 96-well plates. Captiva Pierceable 96-well Collection
A8961007 10
• Prevents cross-contamination Plate Cover
• Simple to use
• Cost effective
This patented vacuum collar is a completely transparent device that joins

Captiva filtration or SPEC SPE 96-well plates directly to our collection
plate. The unique design of the CaptiVac collar forms a preset, prealigned
vacuum seal between the SPE or filtration device and the collection plate,
which positions the outlet tips a specified distance into the collection
plate to prevent cross-contamination of samples. The device is so compact,
simple, and cost effective that a lab can be outfitted with many of these
collars for the cost of a single standard manifold. CaptiVac makes 96-well
SPE and filtration simple, practical, and affordable for any size laboratory.
CaptiVac Vacuum Collars

CaptiVac Vacuum Collar A796 1
CaptiVac Vacuum Collar for Hamilton
A796H 1
MICROLAB ATplus 2
CaptiVac Vacuum Collar Accessories

CaptiVac Gasket Kit A796G 5

54 Varian Syringe Filters
Varian Syringe Filters
• Available in 4, 17 and 30 mm diameters
• Pore sizes from 0.2 to 1.2 µm
• Nylon, PTFT, polypropylene, regenerated cellulose and PVDF filter materials
• Available with glass fiber prefilters
• Hydrophobic and hydrophilic membranes
• Low and high protein binding membranes
A wide variety of disposable syringe-tip filters are available for applications requiring the highest
quality membrane-based devices. Use the following selection guide for selecting the appropriate
syringe filter materials:
Hydrophilic
Nylon — naturally hydrophilic, high protein binding, high mechanical stability, and high dirt loading capacity.
Often used for samples directly injected into HPLC sample loops.
Polypropylene (PP) — hydrophilic, low protein binding, and designed for aqueous and organic solvent based samples.
This filter has low extractables and provides exceptionally low detection levels.
Regenerated Cellulose (RC) — hydrophilic, solvent-resistant membrane. It is a very low protein binder and is ideal
for filtration of proteins, peptides and other biomolecules.
PVDF (polyvinylidene difluoride) — hydrophilic membrane suitable for most general-purpose filtration applications.
It is a low protein binder and has a broad chemical compatibility.
Hydrophobic
PTFE (Teflon®) — hydrophobic, low protein binding, recommended for HPLC solvent or gas chromatography filtration,
aggressive solutions, and small venting applications. Requires prewetting for aqueous samples
Glass fiber prefilters are recommended when samples have high particulate matter to reduce filter bed clogging.
Popular sizes are available in 500 filter packs.
Varian Syringe Filters

Diameter Quantity
Filter Material Prefilter 0.2 µm 0.45 µm 1 µm 1.2 µm
(mm) (/Pk)
Nylon No 100 A4101 A410
PTFE No 100 A4103 A4102
4
PVDF No 100 A4136
RC No 100 A4140 A4139
Nylon No 200 A4131 A4130
200 A4135 A4133
PTFE No
17 500 A4170
PVDF No 200 A4137
RC No 200 A4142 A4143
Glass Fiber 100 CP201939 CP201940
Nylon 100 A4105 A4104 A4109
No
500 A4132
PP No 100 CP201944 CP201945
Glass Fiber 100 CP201950 CP201951
30
PTFE 100 A4107 A4106 A4129
No
500 A4134
Glass Fiber 100 CP201955
PVDF
No 100 A4138
RC No 100 A4145 A4144
Diatomaceous Earth Products 55
Combilute Chem Elut™
Combilute is a 96-well SPE plate filled with • Great for purifying organic synthesis mixtures
Hydromatrix-brand diatomaceous earth. • Simple method for bioanalytical sample preparation
Each well is filled with approximately 250 • No emulsions
mg Hydromatrix and sealed with a • Gravity flow
piercable sealing mat. • Buffered and nonbuffered versions available
• Automatable
The product was specifically designed for
drug discovery and pharmacokinetics laboratories performing:
• Purification of combinatorial libraries
• Screening for pharmaceuticals from biological fluids
Preparation
Combinatorial Chemistry:
Sample
Combilute is an ideal purification tool for combinatorial libraries.
Remove polar reaction byproducts, reagents, and impurities. No vacuum
manifold is required and the use of any standard robotic liquid handler
can be used.
Pharmaceutical Drug Screening:

Drug screening of plasma or other biological fluid samples can be Chem Elut cartridges are ideal for purification or sample preparation.
efficiently performed with Combilute. In studies where a large number
of patient samples must be quickly analyzed, the 96-well format of For sample preparation of biological samples, select the cartridge which
Combilute is indispensible. Robotic liquid handling systems, already used fits the total volume of the sample (including any buffers or additives).
for many other functions in pharmaceutical labs, can be easily adapted to Simply load the sample; wait 3-5 minutes; then elute with organic solvent.
dispense 96 samples quickly into a single Combilute plate, positioned on This general method is ideal for LC/MS sample preparation.
top of a deep well collection plate. No vacuum manifold is required. And
the entire procedure, from sample application to elution, can be easily and For organic reaction mixture purification, simply load the cartridge with
fully automated. aqueous acid to scrub amines (or add base to scrub acids). Select cartridge
size based on the total volume of aqueous buffer to be used. Then pass
Sorbent Mass Quantity the crude synthesis mixture through the cartridge. Gravity flow is all that’s
Sorbent Phase Part No. needed – no vacuum manifolds or pressure required. It’s the fast, easy,
(mg) (Plate)
200 Hydromatrix 65401507 1 reproducible way to scrub organic synthesis mixtures of excess reagents
and reaction byproducts!
Hydromatrix™ Buffered pH
Volume
Part No.
Quantity
(mL) (/Pk)
• High purity diatomaceous earth 3 12198004 100
• Available in straight-barrel 4.5
100 12198018 25
SPE cartridges
9.0 3 12198005 100
• Available in 96-well plates
0.3 12198001 100
• Available in VersaPlate format
• Available in bulk 1 12198002 100
3 12198003 100
Hydromatrix is a high-purity, inert 5 12198006 100
diatomaceous earth available in a variety Unbuffered 10 12198007 100
of convenient configurations with a multitude of timesaving uses. 20 12198008 100
50 12198009 50
Chem Elut products contain Hydromatrix in convenient, ready-to-use 100 12198010 25
cartridges. Chem Elut is ideal for rapid, general sample preparation of bio- 300 15
12198011
analytical samples (plasma, serum, whole blood, urine). Chem Elut can also
be used for purifying organic synthesis mixtures.
For 96-well sample prep or reaction work-up, Combilute contains

Hydromatrix in a standard 96-well plate. Hydromatrix is also available
in bulk quantities for extra large-scale purification, or for SFE or ASE
applications. Did you know Chem Elut Cartridges are packed
with Hydromatrix brand diatomaceous earth?
Item Name Quantity Part No.
1 kg 198003
Hydromatrix
4 kg 198004
Combilute 96-well Plate, Hydromatrix 1 Plate 65401507
Versaplate 96-well-Hydromatrix, 260 mg 1 Plate 75430260
VersaPlate Well Plate Tubes,
96 /Pk 75530260
HYDROMATRIX, 260 mg, 96/pk

56 Flash Chromatography
MetaFlash® Cartridges
Guaranteed trouble-free purification
Your samples and time are valuable. You can save both by purifying your synthesis mixtures on a MetaFlash cartridge. These rugged and dependable
cartridges are fully compatible with your Biotage® Flash instruments and provide the added advantage of a guaranteed leak-free design that preserves
every drop of your samples.
Your single source for a full range of sorbents and cartridge sizes
In addition to the cartridges listed here, we supply SCX, NH2, Diol, and many other bonding chemistries. Please contact us for details and ordering infor-
mation.
Because we maintain a large inventory of MetaFlash cartridges, you don’t have to. We can ship our full line of cartridges in most popular sizes, including
12, 25, 40, 65, 75, and 150 mm cartridges, within 24 hours. For details on how much a just-in-time (JIT) program and single-sourcing can reduce your
costs, please contact your local Varian office.
The highest quality sorbents for the highest level of purification

Clean, reproducible purification requires superior sorbent. Ultra-clean Varian sorbents provide the lowest residue levels to deliver the highest level of
purification. Fast, reproducible flow is achieved with precisely controlled particle sizing. An extensive, well-documented quality program (ISO 9001:2000
certified) ensures lot-to-lot and within-lot reproducibility.
MetaFlash Cartridges - Cylindrical MetaFlash Cartridges - Nozzle Tip

Sorbent Diameter Sorbent Length Quantity Sorbent
Part No. Sorbent Diameter Length
Phase (mm) Mass (cm) (/Box) Mass Quantity Part No.
Phase (mm) (cm)
4g 7.5 2 A850212S2 (g)
12 12 /Box A850040S
8g 15 2 A850212M2 45 7
45 g 7 1 A850240SE 144 /Box A850040SJ
40 12 /Box A850040M
100 g 15 1 A850240ME 40 100 15
C18 200 g 9 1 A850275SE Silica 144 /Box A850040MJ
75 400 g 15 1 A850275ME 12 /Box A850040L
130 20
800 g 30 1 A850275LE 144 /Box A850040LJ
2.5 kg 30 1 A8502150ME 65 350 20 6 /Box A850065I
150
5 kg 60 1 A8502150LE
C8 75 5 kg 60 2 A850375LE
20 A850012S
4g 7.5
100 A850012SJ
12
20 A850012M
8g 15
100 A850012MJ
20 A850025S
22 g 7.5
100 A850025SJ
25
20 A850025M
41 g 15
100 A850025MJ
12 A850040SX
45 g 7
144 A850040SXJ
40
12 A850040MX
Silica 100 g 15
144 A850040MXJ
2 A850075S
200 g 9
10 A850075SJ
2 A850075M
75 400 g 15
10 A850075MJ
2 A850075L
800 g 30
10 A850075LJ
2 A8500150M
2.5 kg 30
10 A8500150MJ
150
2 A8500150L
5 kg 60
10 A8500150LJ
Flash Chromatography 57
Mega Bond Elut® Flash
• Convenient: disposable Bond Elut cartridges eliminate the need for packing glass columns
• Flexible: “open” tube design for both liquid and solid samples
• Reliable: consistent, high resolution performance backed by Varian’s ISO 9001 quality systems
Varian’s line of Mega Bond Elut Flash cartridges offers superior levels of performance and productivity for the flash purification of organic compounds
from both aqueous and nonaqueous solutions. Prepacked, disposable cartridges offer greater convenience than glass columns that have to be washed,
dried and repacked after every run.
Compatibility with parallel automation systems ensures high throughput in cases where maximum productivity is required.
High-yield Separations - milligrams to grams
Preparation
With the Varian Mega Bond Elut Flash cartridges, high-yield separations of milligram to gram samples are quickly and easily achieved. Packing materials
Sample
are based on the Bond Elut 40 µm particles. These cartridges exhibit excellent efficiency due partly to the narrow pore size range of the sorbents.
Varian Mega Bond Elut Flash cartridges are available in a wide range of chemistries. To optimize the purification and economy of your separation, we
provide a range of cartridge sizes and sorbent types:
Cartridge sizes: 12, 20, 60 and 150 mL
Chemistries: C18, NH2, SCX and SI
Bed masses: 2g to 70g
Compatibility with Flash Systems

- Mega Bond Elut Flash cartridges are compatible with several purification systems including:
- Anachem FCM-Single and FCM-10
- Argonaut Flash Master and Flash Pack
Mega Bond Elut Flash Cartridges

Sorbent
Volume
Sorbent Phase Mass Quantity 40 µm
(mL)
(g)
1 60 16 /Box 12256060
2 12 8 /Box 12256015
5 20 8 /Box 12256023
10 60 8 /Box 12256031
C18
20 60 16 /Box 12256078
25 150 8 /Box 12256079
50 150 8 /Box 12256080
70 150 8 /Box 12256081
2 12 8 /Box 12256020
5 20 16 /Box 12256028
10 60 16 /Box 12256036
NH2 20 60 16 /Box 12256074
25 150 8 /Box 12256075
50 150 8 /Box 12256076
70 150 8 /Box 12256077
20 60 16 /Box 12256065
25 150 8 /Box 12256070
SCX
50 150 8 /Box 12256072
70 150 8 /Box 12256073
2 12 8 /Box 12256018
5 20 8 /Box 12256026
10 60 8 /Box 12256034
20 150 8 /Box 12256042
SI
15 60 16 /Box 12256068
25 150 8 /Box 12256069
50 150 8 /Box 12256067
70 150 8 /Box 12256071

58 Bulk SPE Sorbents
Bondesil® Bulk Sorbents
The same Bondesil sorbent packed into the industry-leading Bond Elut SPE cartridges is available in bulk for large-scale purification or packing custom
cartridges. The Bondesil family of bonded silica sorbents is specifically designed for ultra-selective chemical separation such as:
• Custom packing of SPE columns

• Flash chromatography
• Large-scale preparative separations
• HPLC column switching systems
• Matrix Solid Phase Dispersion
• Low pressure chromatographic systems
• Batch extraction from large-volume samples
Bondesil Bulk Sorbents Bondesil Bulk Sorbents (continued)

Particle Particle
Sorbent Phase Size Quantity Part No. Sorbent Phase Size Quantity Part No.
(µm) (µm)
10 g 12213029 100 g 12214013
FL 200
2OH 40 100 g 12213030 1 kg 12214015
1 kg 12213031 LMS 75 100 g 12216060
Alumina-N 25 1 kg 12213073 10 g 12213020
10 g 12213002 40 100 g 12213021
NH2
C1 40 100 g 12213003 1 kg 12213022
1 kg 12213004 120 100 g 14213021
10 g 12213011 5g 12213045
PBA 40
40 100 g 12213012 10 g 12213044
C18 1 kg 12213013 10 g 12213014
100 g 14213012 PH 40 100 g 12213015
120
1 kg 14213013 1 kg 12213016
C18 OH 40 100 g 12213049 PPL 125 100 g 12216062
10 g 12213005 10 g 12213035
C2 40 100 g 12213006 PRS 40 100 g 12213036
1 kg 12213007 1 kg 12213037
10 g 12213008 10 g 12213023
C8 40 100 g 12213009 PSA 40 100 g 12213024
1 kg 12213010 1 kg 12213025
10 g 12213032 10 g 12213041
CBA 40 100 g 12213033 SAX 40 100 g 12213042
1 kg 12213034 1 kg 12213043
10 g 12213017 10 g 12213038
CH 40 100 g 12213018 40 100 g 12213039
SCX
1 kg 12213019 1 kg 12213040
CN-E 40 100 g 12213061 120 100 g 14213039
10 g 12213026 SI 40 500 g 12213001
CN-U 40 100 g 12213027
1 kg 12213028
40 100 g 12213070
Certify
120 100 g 14213070
40 100 g 12213071
Certify II
120 100 g 14213071
10 g 12213046
DEA 40 100 g 12213047
1 kg 12213048
ENV 125 100 g 12216061
2g 12212000
EnvirElut 40 100 g 12214016
1 kg 12214019
SPE Cartridge Processing Stations & Accessories 59
CaptiVac™ Vacuum Collars Positive Pressure Manifold
• For use with Captiva Filtration and 96-Deep Well collection plates, and Positive pressure is often preferable to vacuum for SPE sample processing.
SPEC SPE 96-well plates. This is because:
• Prevents cross-contamination • Positive pressure provides more than two times greater total force for
• Simple to use moving viscous samples than vacuum
• Cost effective • Positive pressure will not dry out SPE cartridges when viscous or other
difficult samples are being processed
• Use of nitrogen or other inert gas for positive pressure applications
can improve recovery and extraction reproducibility or easily -oxidized
analytes
The Varian Positive Pressure Mani-
Preparation
fold ensures fast and reproducible
Sample
parallel processing of up to 24 Bond
Elut cartridges (1, 3, 6, or 10 mL sizes).
Pressure at each cartridge (up to 50
psig) is automatically controlled with
individual valves that shut off flow
when the cartridge is drained. This
prevents faster flowing cartridges
This patent-pending vacuum collar is a completely transparent device that from drying out, so there’s no
joins Captiva filtration or SPEC SPE 96-well plates directly to our collection need for any manual intervention,
plate. The unique design of the CaptiVac collar forms a preset, prealigned such as shutting off stopcocks,
vacuum seal between the SPE or filtration device and the collection plate, which is typically done with
which positions the outlet tips a specified distance into the collection vacuum manifolds. The Varian
plate to prevent cross-contamination of samples. The device is so compact, Positive Pressure Manifold is constructed of rugged stainless steel and
simple, and cost effective that a lab can be outfitted with many of these solvent resistant components that provide many years of trouble-free use.
ii
i
i
collars for the cost of a single standard manifold. CaptiVac makes 96-well
SPE and filtration simple, practical, and affordable for any size laboratory.
ii
i
Positive Pressure Manifold
Quantity
CaptiVac Vacuum Collars (Each)
Positive Pressure Manifold with 10 x 75 mm,
Description Part No. Quantity 12234201 1
collection rack, complete system
CaptiVac Vacuum Collar A796 1
CaptiVac Vacuum Collar for Hamilton 12234200 1
A796H 1 collection rack, complete system
MICROLAB ATplus 2
12234202 1
CaptiVac Vacuum Collar Accessories 12234203 1
CaptiVac Gasket Kit A796G 5
Both sets of samples generated by precipitating bovine plasma with acetonitrile (1:4, v/v)
Centrifugation performed at 3000 rpm for 5 minutes.
HPLC gradient: 10% acetonitrile (0.5 min), ramp to 60% acetonitrile (0.5 min), hold for 2
min, ramp to 10% acetonitrile (0.5 min)
HPLC column: Polaris C18-A, 3 um, 2 x 50 mm.
Injection - 100 uL

60 SPE Cartridge Processing Stations & Accessories
Positive Pressure Manifold Replacement Vac Elut® 20 Manifold
Components and Accessories
Engineered to increase laboratory productivity, the corrosion-resistant Vac

Elut 20 allows simultaneous processing of up to 20 Bond Elut cartridges.
The manifold’s clear glass base allows careful monitoring of the entire
ii
ii
i
sample collection process. Its compact, linear design requires very little
Positive Pressure Manifold Replacement benchtop space.
Item # Description Part No.
Quantity • Clear glass base
(Each) • Disposable needles eliminate cross contamination
1 Pressure Regulator 12234204 1 • Increased productivity/sample throughput
Collection Rack for 10 x 75 mm, • Rugged, reliable construction
12234211 1
test tubes • Simple vacuum adjustment
Collection Rack for the 12 x 75
12234210 1
• Multiple rack options
mm, test tubes
2
Collection Rack for 13 x 100 Rugged construction
12234212 1
mm, test tubes On the Vac Elut 20, the vacuum-control valve, vacuum gauge, and quick-
Collection Rack for 16 x 100 release valve are mounted on the lid, away from the corrosive solvent
12234213 1
mm, test tubes stream and within convenient reach. The solvent-resistant polypropylene
3 Waste Collection Tub 12234231 1 rack is available in a variety of sizes to accommodate the collection tubes
4 Silicone Rubber Gasket 12234230 1 commonly used in sample preparation.
Adapter Plate for 1 mL Bond
12234220 1
Elut Cartridge Freedom from sample contamination
Adapter Plate for 3 mL Bond To minimize the risk of sample carryover, low-cost, disposable, medical-
12234221 1
Elut Cartridge grade polypropylene delivery needles can be easily replaced. Polypropylene
5
Adapter Plate for 6 mL Bond extender tips are also available as a replacement for the standard needle
12234222 1
Elut Cartridge valves, ensuring a direct path into the collection tube. Correct sample
Adapter Plate for LRC, 10 mL identification is also ensured by an interlocking fit between the lid and
12234223 1
Bond Elut Cartridge
internal test tube rack.
ii
ii
Vac Elut 20 Manifold

Quantity
(Each)
Vac Elut 20 Manifold with Collection Rack
12234105 1
for 10 x 75 mm, test tubes
12234100 1
12234101 1
12234102 1
12234103 1
SPE Cartridge Processing Stations & Accessories 61
Vac Elut® 20 Manifold - Tall Glass Basin Vac Elut® 20 Manifold Replacement
ii
ii
i
Vac Elut 20 Manifold Replacement

Description Part No. Quantity Image
Polypropylene
12234511 25 /Pk
Delivery Needles
Replacement
Exit Valve for 12234506 1 Each
Glass Basin
Preparation
Replacement Lid
12234502 1 Each
Gasket
Sample
Varian offers a Vac Elut 20 with a large glass basin and collection rack
that accommodates the larger 16 x 150 mm test tubes (on the left of the
photo). The same high quality material and features on the standard Vac Vac Elut 20 Lid
12234501 1 Each
Elut system are incorporated on this special unit. These larger collection Cover
vessels can be utilized in combinatorial chemistry applications using large
boiling tubes for collection of purified synthesis mixtures, or for any SPE Vacuum Gauge
12234504 1 Each
extraction in which an elution volume greater than 10 mL is required.
ii
ii
Assembly
Quantity
(Each)
Vac Elut 20 Manifold with the Tall Glass
Basin and Collection Rack for 16 x 150 mm, 12234104 1 Vac Elut® SPS 24 Manifold
test tubes, complete system
The Vac Elut SPS 24 allows you to process up to 24 SPE cartridges
Collection rack for 10x75 mm Test Tubes 12234517 1
simultaneously. Like all Vac Elut manifolds, the SPS 24 is made from
durable, solvent-resistant materials and engineered to last. The glass sides
allow easy viewing of the entire sample collection process.
Vac Elut® 20 Manifold Racks and Glass Basins The highlighting feature of the SPS 24 system is its waste diversion
ii
ii
funnel that enables all steps of the SPE procedure to be completed without
i
Vac Elut 20 Manifold Racks and Glass Basins

removing the lid. The collection rack can be placed inside the unit before
Quantity
Description Part No. Image the extraction begins. This unique characteristic of the SPS 24 eliminates
(Each)
splash-back, prevents cross contamination, and minimizes hazardous
waste, and biohazard exposure.
Standard Glass Basin 12234505 1
Complete with replacement stainless steel delivery tips for maximum
Tall Glass Basin 12234512 1 extract purity, the Vac Elut SPS 24 system also includes a vacuum
Collection rack for controller/release, collection rack, and port sealing plugs. Racks for several
13 x 75 mm, test 12234507 1 different collection tube configurations are available.
ii
i
tubes
i
Collection Rack for Vac Elut SPS 24 Manifold

13 x 100 mm, 12234508 1 Quantity
test tubes (Each)
Vac Elut SPS 24 Manifold with
Collection Rack for 12234003 1
Collection Rack for 10 x 75 mm, test tubes
16 x 75 mm, test 12234509 1 Vac Elut SPS 24 Manifold with
tubes 12234041 1
Collection Rack for Vac Elut SPS 24 Manifold with
16 x 100 mm, 12234510 1 12234022 1
test tubes Vac Elut SPS 24 Manifold with
12234004 1

62 SPE Cartridge Processing Stations & Accessories
Vac Elut® SPS 24 Replacement Components
i
i
ii
i
Vac Elut SPS 24 Replacement Components

Collection Rack and Funnel Set for
12234026 1 Each
10 x 75 mm, test tubes
12234027 1 Each
12 or 15 mL Conical Tubes
12234030 1 Each
12234031 1 Each
12234028 1 Each
Elastic Lid Fasteners - 6/pk 12234034 6 /Pk
Lower Lid Seals - 2/pk 12234050 2 /Pk
SPS 24 Glass Basin 12234024 1 Each
SPS 24 Lid Cover 12234025 1 Each
SPS 24 Spare Parts Kit 12234033 1 Each
SPS 24 Waste Tower Repair Kit, Base Exit
Tube, Hose Connector, Washer, Center 12234005 1 Each
Tube, 90º Connector Elbow
Solvent Resistant Vacuum Gauge 12234023 1 Each
Stainless Steel Delivery Needles - 25/pk 12234038 25 /Pk
Upper Lid Seals - 2/pk 12234051 2 /Pk
Vac Elut SPS 24 Lid Screw Kit - 12/pk 12234021 12 /Pk
Waste Funnel for 10 x 75 mm, test tubes 12234035 5 /Pk
Waste Funnel for 12 x 75 or 13 x 100 mm,
12234032 5 /Pk
test tubes - 5/pk
Waste Funnel for 16 x 100 mm, test tubes 12234029 5 /Pk
Welch Vacuum Chemical Duty

Vacuum Pumps
• PTFE chemical duty pumps
• Free air displacement from 22 to 45 L/min
• Exceed performance of water aspirator
• Recommend 19/22 L/min for VacElut 20, SPS 24
• Recommend 38/45 L/min for VersaPlate, EnvirElut
Welch Vacuum Chemical Duty Vacuum Pumps

Voltage Description Part No. Quantity
22L/min at 26.7”Hg:
WL2012B01 1
115V/60Hz
115V/60Hz
WL2015B01 1
115V/60Hz
WL2012C02 1
230V/50Hz
230V/50Hz
WL2015C02 1
230V/50Hz
Solid Phase Extraction Publications 63
Handbook of Sorbent Extraction Solid Phase Extraction: Principles,
Technology Techniques, and Applications
Preparation
Sample
Varian offers the internationally acclaimed Handbook of Sorbent
Extraction Technology. This handbook, now in its third edition, is the
most comprehensive guide to method development available, containing
easy-to-follow information on: If you require a complete reference resource of Solid Phase Extraction
• logical, efficient method development technology, then you will want to get the new textbook published by
• troubleshooting tips for improving analyte recovery and method Marcel Dekker Inc. and offered by Varian. It is the only comprehensive
reproducibility textbook that covers the broad field of SPE. No one person is expert
• technical discussions on the nature of bonded silica sorbents in all aspects of the application of SPE. Therefore this book contains
• practical examples of SPE applications using all the commonly submissions from a variety of independent experts in each field. Packed
encountered mechanisms: non-polar, polar, ion exchange, covalent, with over 500 pages of expertise, it contains a complete index, over a
and mixed-mode thousand references and several hundred diagrams and chromatograms.
• effective clean up of difficult samples such as foodstuff, soil or tissues
• Indices of functional group pKas, counter-ion selectivities, and much Description Part No. Quantity
more. Solid-Phase Extraction, Principles,
PUBSPE001 1
Techniques, and Applications
The Handbook of Sorbent Extraction Technology has proven to be an
invaluable reference source for chemists worldwide. Over 15,000 copies
have been sold globally.
Solid Phase Microextraction
Quantity
Item Name Item Code
(Each)
Handbook of Sorbent Extraction
12245005 1
Technology
This comprehensive reference contains extensive descriptions of proven

sampling methods for chemical analyses. The book focuses on applications
of solid phase microextraction (SPME) for laboratories analyzing dirty
sample matrices and requiring high sample throughput, quick sample
turnaround time, and low detection levels. Provides a sound theoretical
basis for SPME, plus highly detailed descriptions of pharmaceutical,
environmental, food science, forensic, and toxicological applications.

Solid Phase Microextraction PUB0824770587 1

64 Solid Phase Microextraction
SPME Fibers
SPME fiber assemblies can be reused for up to 100 analyses or more, depending on the application and the care they are given. For reuse, simply condition
with solvent or heat before and after every analysis. Each assembly has a color-coded or notched hub indicating the type of coating on the fiber. Choose
the assembly that is appropriate for the holder: manual, or Varian AutoSampler (also used for HPLC applications). First time SPME users must order both
a holder and a fiber assembly.
Recommendations:
For gases and low molecular weight compounds (MW from 30 to 225):
- Carboxen/PDMS fibers
For volatiles (MW from 60 to 275):
- 60 µm PDMS fibers
For semivolatiles (MW from 80 to 300):
- polyacrylate fibers
For nonpolar high molecular weight compounds (MW from 125 to 600):
- 7µ PDMS fibers
For nonpolar semivolatiles (MW from 80 to 500):
- 30 µm PDMS fibers
For alcohols and polar compounds (MW from 40 to 275):
- Carbowax/DVB fibers
For flavors (MW from 40 to 275):
- Carboxen/DVB/PDMS on a StableFlex fiber
For trace levels (MW from 40 to 275):
- Carboxen/DVB/PDMS on a 2 cm StableFlex fiber
SPME Kits offer a convenient assortment of fibers for method development and first-time sample analysis.
SPME Fibers
3 per pack
Length Fiber OD
Inlet Usage Coating Fused Silica StableFlex Gauge
(cm) (µm)
65 391896312 24
Carbowax/DVB 1
70 SU57337U 24
Carbowax/PDMS 1 85 SU57335U 24
Carboxen/DVB/PDMS 1 50/30 SU57329U 24
Carboxen/PDMS 1 75 391896316 24
A/S
7 391896303 24
PDMS 1 30 391896310 24
100 391896302 24
PDMS/DVB 1 65 391896314 SU57327U 24
Septum Polyacrylate 1 85 391896306 24
Carbowax/DVB 1 65 391896311 24
1 50/30 SU57328U 24
Carboxen/DVB/PDMS
2 80 SU57348U 24
Carboxen/PDMS 1 75 391896315 24
Manual 7 391896304 24
PDMS 1 30 391896309 24
100 391896301 24
PDMS/DVB 1 65 391896313 SU57326U 24
Polyacrylate 1 85 391896305 24
Carbowax/DVB 1 70 SU57339U 23
Carboxen/PDMS 1 75 SU57343U 23
A/S
PDMS 1 100 SU57341U 23
PDMS/DVB 1 65 SU57345U 23
Merlin Microseal
Carbowax/DVB 1 70 SU57338U 23
Carboxen/PDMS 1 75 SU57344U 23
Manual
PDMS 1 100 SU57342U 23
PDMS/DVB 1 65 SU57346U 23
DVB = divinylbenzene. PDMS = polydimethylsiloxane
Solid Phase Microextraction 65
SPME Kits
Quantity Fiber OD Length
Inlet Usage Item Name Part No. Gauge
(/Pk) (µm) (cm)
Kit 1, PDMS, 85 µm, 100 µm, 7 µm, Automated Sampling 391896308 3 7, 85, 100 1 24
Kit 2, Carbowax/DVB, PDMS/DVB, Automated Sampling SU57321U 3 65, 75 1 24
A/S Kit 3 for HPLC, Carbowax/TPR, PDMS/DVB, Automated Sampling SU57323U 3 50, 60, 100 1 24
Kit 4 PDMS, PDMS/DVB, PDMS/ Carboxen, Automated Sampling SU57325U 3 50, 65, 75 1 24
Septum StableFlex Fiber Assortment Kit, Automated Sampling SU57551U 4 50/30, 65,70, 85 1&2 24
Kit 1, PDMS, 85 µm, 100 µm, 7 µm, Manual Sampling 391896307 3 7, 85, 100 1 24
Kit 2, Carbowax/DVB, PDMS/DVB, Manual Sampling SU57320U 3 65, 75 1 24
Manual
Kit 4, PDMS, PDMS/DVB, PDMS/Carboxen, Manual Sampling SU57324U 3 50, 65, 75 1 24
StableFlex Fiber Assortment Kit, Manual Sampling SU57550U 4 50/30, 65,70, 85 1&2 24
Preparation
Sample
SPME Accessories
Quantity
Item Name Part No.
(Each)
Merlin Microseal Replacement for SPME, 23GA 392609902 1
Merlin Microseal SPME Kit for 1079 Injector, 23 GA 392609901 1 Kit
Merlin Microseal SPME Kit for 1177 Injector, 23 GA 392609903 1 Kit
SPME 15 mL Stand SU57357U 1
SPME Fiber Holder for CTC Autosampler SU57347U 1
SPME Fiber Holder for Manual Sampling 391896401 1
SPME Fiber Holder for Varian Autosampler, or HPLC analysis SU57331 1
SPME Inlet Guide for Manual Injection. Fits most Varian and Agilent injectors. SU57356U 1
SPME Link Septa, 11 mm 392548402 25 /Pk
SPME Sensitivity Test Sample 391896700 5 /Pk
Solid Phase Microextraction
This comprehensive reference contains extensive descriptions of proven sampling methods for chemical analyses. The book focuses on applications of solid
phase microextraction (SPME) for laboratories analyzing dirty sample matrices and requiring high sample throughput, quick sample turnaround time, and
low detection levels. Provides a sound theoretical basis for SPME, plus highly detailed descriptions of pharmaceutical, environmental, food science, forensic,
and toxicological applications.

Solid Phase Microextraction PUB0824770587 1

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Varian Sample Preparation Products 1

Quality and Commitment for Over 25 Years

• TOP: a simple, cost-effective, high-throughput solution for Trityl-on Oligonucleotide Purification.

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Alumina, like silica, is an extremely polar sorbent. The alumina

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SPEC C8 offers similar selectivity to Bond Elut C8.

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OMIX Pipette Tips

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MPC-Mixed Phase Cation SD

Empore Extraction Disks

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Cyclohexyl is a medium polarity sorbent that exhibits unique selectivities

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Bond Elut CN-U cyanopropyl sorbent