By Nguyen Hoang Viet, PhD.

ANALYZE PARTICLES
BY USING IMAGEJ
http://rsb.info.nih.gov/ij/docs/menus/analyze.html#ap
Content
 Where to download
 How to run ImageJ
 Image Processing & Calculation
Where to download
Visit this link:
http://rsbweb.nih.gov/ij/download.html

Note: please check your window first, 32- or

64-bit system
How to run ImageJ
First – Run ImageJ program and Load the
your image file.
 Open Image File
 Run ImageJ: Start/All Programs/ImageJ/

 Open Image File: File/Open…, choose the file

and click OK
Image Processing & Calculation
Step 1 – Convert to 8-bit & Crop
 Open Image File: File/Open…, choose the file
and click OK - Grain Size Calculation.jpg
 Convert to 8-bit: Image/Type/8-bit.
 Crop: Draw a rectangular shape then
Image/Type/Crop.
 Save the cropped file: File/Save as/Jpeg… -
Grain Size Calculation-Crop.tif
Measurement scale unit
 Draw a rectangular shape fits on scale bar. You
will see its width to be 237 px.

 So, 1 m equals 79px.

Step 2: Convert to grainy image
 Image/Adjust/Threshold:
Other processing:
- Process/Binary/Erode
- Process/Binary/Dilate
Step 3: Analyze Particles
 Analyze/Analyze Particles …
 There are two data files:
 Results.xls

 Summary.xls

 Average grain size:

 708 px2= 0,113 m2.
How to set new scale bar
 Analyze/Set Scale

 Then, the unit in imageJ

will be µm (micrometer)
Step 4: Analyze data
 Based on two data files in Excel format, you
should find a software for doing further
analysis. Such software like: Excel, MatLab,
Origin, SciLab*, SciDAVis* …

*: free software
Step 4: Analyze data (2)
 On the ‚Results‛ dialog. Choose
Results/Distribution… the set bin number for
‘specify bins’ field: 10, 50 and 100.
 Based on Histogram dialog
Click on List to get data list,
then you should use other
software to draw the graph.
Histograms of the grain sizes
Grain sizes of cropped images
Acknowledgement
 Thank my students, ATP k52 (Fall 2010), for
doing well this homework and have good
suggestion for further processing.
Discussion time!
Any questions?
Please email me at viet4777@gmail.com for further information