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Station 2
1. Give groups their starting vial, containing a potent solution of water and food coloring
2. Students will have a quick run through of serial dilution and how to use a micropipette.
3. Students will then dilute their colored solution by adding 1mL (1000uL) of the colored
water into a new bottle with 6mL of water.
4. Students will be tasked to serial dilute the solution two more times by adding 1mL of the
previous solution made to 6mL of water in a brand new bottle.
5. Students will successfully complete the activity when the solution is correctly diluted, and
there are four bottle of their colored solution that consecutively get more pale.
Station 3
● During the activity, the instructor can talk about the parts of the microscope, and how to
properly focus a slide on the microscope.
○ Eyepiece, Objective Lenses, Stage, Base, Light, Coarse Adjustment Knob, Fine
Adjustment Knob, Diaphragm, Light Intensity Knob, Stage Adjustment Knobs.
● To properly focus a compound microscope, you must start at the lowest objective lens
(4x) and use the coarse adjustment knob to focus. Once the slide is completely focused,
slide to the next highest objective lens (10x) and refocus using the fine adjustment knob.
If further magnification is needed, you can move up to the 40x lens and refocus again
using the fine adjustment knob.
● The eyepiece contains a lens whose magnification is 10x. The formula to calculate the
total magnification is: The Total Magnification = Ocular Lens × Objective Lens.
● Microscopes are important for research because it allows us to study microscopic
organisms and other things that are not visible by the naked eye that can be harmful to
us like some bacteria.
Station 4
Students will have learned how to use a compound microscope at Station C. Two more
prepared will be available for the students to identify. Students will look learn about
stereoscopes and why they are different than compound microscopes. Under the 4
stereoscopes, students will be able to look at pennies, dead insects and live insects.
yourself at home!