Name: ema physioex

Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report

Pre-lab Quiz Results

You have not completed the Pre-lab Quiz.

02/15/19 page 1
Experiment Results
Predict Question:
Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane?
Your answer : c. No, not at all.

Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average
molecular weight of a single amino acid is 135 g/mole.

Which of the following will be able to diffuse through the 200 MWCO membrane?
Your answer : c. glucose only

Stop & Think Questions:

The reason sodium chloride didn't diffuse left to right is that
You correctly answered: c. the membrane pore size was too small.

Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino
acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because
You correctly answered: d. glucose and albumin are both too large to pass.

The rate of diffusion for urea

You correctly answered: b. is slower than that for sodium because urea is a larger molecule.

Experiment Data:

Solute MWCO Left Solute Concentration Right Solute Concentration Average Diffusion
Rate
Na+ Cl- 20 9.00 0.00 0.0000
Urea 20 0.00 0.00 0.0000
Albumin 20 0.00 0.00 0.0000
Glucose 20 0.00 0.00 0.0000
Na+ Cl- 20 0.00 0.00 0.0000
Urea 20 9.00 0.00 0.0000
Albumin 20 0.00 0.00 0.0000
Glucose 20 0.00 0.00 0.0000
Na+ Cl- 50 9.00 0.00 0.0150
Urea 50 0.00 0.00 0.0000
Albumin 50 0.00 0.00 0.0000
Glucose 50 0.00 0.00 0.0000
Na+ Cl- 50 18.00 0.00 0.0273
Urea 50 0.00 0.00 0.0000
Albumin 50 0.00 0.00 0.0000
Glucose 50 0.00 0.00 0.0000
Na+ Cl- 100 9.00 0.00 0.0150
Urea 100 0.00 0.00 0.0000
Albumin 100 0.00 0.00 0.0000
Glucose 100 0.00 0.00 0.0000
Na+ Cl- 100 0.00 0.00 0.0000
Urea 100 9.00 0.00 0.0094

02/15/19 page 2
Solute MWCO Left Solute Concentration Right Solute Concentration Average Diffusion
Rate
Albumin 100 0.00 0.00 0.0000
Glucose 100 0.00 0.00 0.0000
Na+ Cl- 200 0.00 0.00 0.0000
Urea 200 0.00 0.00 0.0000
Albumin 200 0.00 0.00 0.0000
Glucose 200 9.00 0.00 0.0042
Na+ Cl- 200 0.00 0.00 0.0000
Urea 200 0.00 0.00 0.0000
Albumin 200 9.00 0.00 0.0000
Glucose 200 0.00 0.00 0.0000

02/15/19 page 3
Post-lab Quiz Results
You scored 0% by answering 0 out of 4 questions correctly.

1. The effect of increasing the concentration of sodium chloride from 9 mM to 18 mM in the left beaker was to
You did not answer this question.

Correct answer: b. increase the rate of diffusion.

2. Describe the difference between the rate of diffusion seen for sodium and urea.
You did not answer this question.

Correct answer: c. Urea diffused more slowly because it is larger than sodium.

3. Which of the following solutes did not pass through any of the membranes?
You did not answer this question.

Correct answer: b. albumin

4. When diffusion stops, we say the solution has reached

You did not answer this question.

Correct answer: a. equilibrium.

02/15/19 page 4
Review Sheet Results
You have not completed the Review Sheet.

02/15/19 page 5