Protein Structure Analysis

Our understanding of life at the molecular level is highly dependent on the ability
to map the molecular details of individual proteins and nucleic acids as well as
their interactions with each other and with small molecules (inhibitors, cofactors,
substrates, etc.). The determination of 3-D structures of proteins is crucial for the
understanding of these interactions as well as their structure–function
relationships, which also has many practical applications in drug design and
protein engineering.
Structure determination is usually a tedious and expensive process whereby the
target macromolecule must be produced in relatively large quantities and purified
in high concentrations. A successful experiment often requires expert knowledge
of biochemistry, bioinformatics and molecular biology. Over the past decades,
rapid technical advances in DNA cloning, protein expression and purification
methodologies have made it generally easy to obtain enough amount of proteins
for their structural study. Besides, numerous innovations and improvements have
been made in solving protein structures using instrumental methods such as X-
ray crystallography, nuclear magnetic resonance (NMR) spectroscopy and
electron microscopy (EM). These efforts together have given rise to dramatic
accumulation of 3-D structural data in the past few decades (Figure 1). The large
number of protein structures will certainly yield valuable information to the rules
for elucidating protein folding/unfolding mechanisms and understanding their
biological functions.

Figure 1. The growth of total available structures deposited in PDB since 2000.
Creative Biostructure has a team of scientists who have rich experience in
solving the structure of various protein targets, such as kinases, polymerases,
chaperones and membrane proteins. Over the years, we have developed four
unparalleled platforms, including X-ray crystallography, NMR, EM and membrane
protein screening, to facilitate your research in structural biology.

Figure 2. Four platforms at Creative Biostructure

By using the most advanced platform apparatus and techniques, Creative

Biostructure promises unbeatable precision in protein structure services with
competitive prices.

 Protein crystallization: Creative Biostructure offers high-throughput

UniCrys™ protein crystallization services with our state-of-the-art facilities.
High-quality protein crystals can be obtained, which are suitable for X-ray
diffraction studies.
 Structure determination: Creative Biostructure employs various strategies for
the structure determination of macromolecules of your interest. Our scientists
are experienced with phase determination of X-ray diffraction
pattern, NMR spectroscopy and cryo-EM. We promise to provides the most
efficient and economical strategies tailored to our customers’ requirement.
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Please feel free to contact us to discuss your project!

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