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INTRDOCUTION
Compound action potential is a signal from
large populations of axons which is recorded
extracellularly. The amplitude of the
compound action potential indicates the
number of firing axons in it. A typical nerve
in a frog consists of thousands of axons. In
addition, the nerves of vertebrates is
enclosed in a connective tissue sheath and
the axons are further divided into bundles
which are called as fasciculi. The fasciculi is
covered by a connective tissue sheath, the
Perineurium. The frog’s sciatic nerve is used
in order to determine the compound action
potential. It consists of only a single bundle
of fibers which is then surrounded by the
perineurium and epineurium. Extracellular
recording is done through placing an
electrode in close proximity to the excitable
nerve, and the reference electrode in the
extracellular fluid. The potential changes in
the membrane surface are recorded. In this
experiment, the objective is to understand
how the absolute and relative refractory
periods of a nerve can be demonstrated and
the physiological significance of these