Professional Documents
Culture Documents
To use the Winkler Titration Method to determine the BOD value of water sample.
APPARATUS:
a) Beakers
b) Conical Flasks
c) Burette
d) Graduated Cylinder
e) Pipette
f) Volumetric Flasks
g) BOD bottles
h) Reagent bottles
i) Incubator
j) Distilled Water
k) Dilution Water
l) Starch solution
m) Sulfuric acid
n) Manganous Sulphate Solution
o) Alkali-iodize-azide reagent
p) Concentrated Sulphuric acid
q) Natrium Thiosulphate Titrant
PROCEDURE:
A. Preparation of sample
1. Prepare 3 litres of Dilution water by adding with per litre of distilled water a 1 ml
of prepared solutions of phosphate buffer, magnesium sulphate (MgSO4), Calcium
Chloride (CaCl2), Ferric Chloride (FeCl3) and Alitiourea (ATU).
2. Aerated the dilution water by shaking it in a partially filled bottle.
3. Label two 1 Litre of volumetric flasks with ‘sample’ and ‘blank’ respectively. The
flask water labelled ‘blank’ is used as control to make sure that the dilution water
is not polluted.
4. Transfer 200 ml of water sample into the flask labelled ‘sample’ and add dilution
water until it reaches the 1 Litre level, which will give a dilution 1/5 of the
sample. Shake the flask thoroughly.
5. To the ‘blank’ flask, transfer 1 Litre of Dilution water.
6. Label 4 clean BOD bottles as 1-4.
7. Fill bottle 1 & 2 with solution from ‘blank’ flask and bottle 3 & 4 with solution
from ‘sample’ flask.
8. Cover the bottles carefully.
9. Determine the initial (day zero) dissolved oxygen value for solution in bottle 1 &
3 using Winkler Titration Method.
10. Incubate bottle 2 & 4 for 5 days ± 1 hour at temperature 20 ± 1˚C.
11. Determine the dissolved oxygen values of solutions in bottle 2 & 4 after 5 days.
RESULT:
Sample type: ___________________________
Dilution Ratio: __________________________
Sample
Sample