Professional Documents
Culture Documents
Date of Submission:04-03-2019
Table of Contents
Theory: .......................................................................................................................................................... 3
Introduction .................................................................................................................................................. 5
Objective ....................................................................................................................................................... 5
Result and calculation: .................................................................................................................................. 6
Discussion: .................................................................................................................................................... 8
Remarks and Comments: .............................................................................................................................. 8
Theory:
When a solute is shaken with two solvents which are immiscible with each other but in both of
which the soluble, then the solute is found to distributed itself between the two solvent in such a
way that the ratio of the concentration of the solute in the solvent is constant at a constant
temperature.
If C1 and C2 are the concentration of the solute in solvent 1 and 2 respectively, then according to
the Nernst distillation law:
C1/C2 = KD
The constant KD is called the distribution coefficient or distribution ratio.
The distribution law is valid when the molecular species of the solute in the two solvents remain
the same, i.e., there is no association or dissociation of the solute in any of the solvents. If the
solute undergoes association in one of the solvents but remains unchanged in the other solvent as
shown in the figure, the law can not be applicable as such since the molecular species have under
zone a change. Using the law of mass action can do modification for this. Considering the
equilibrium between the different molecular species A undergoing association in the solvents. The
following reaction can easily obtain:
nA (A)n
C3 C2
C2/(C3)n = Kꞌ [mass law constant]
(n√C2)/C3 = constant……………….(1)
Again, C1/C2 = constant…………………(2)
Dividing equation (2) by (1) we get,
(C1/C3)/(n√C2√C3) = constant
C1/(n√C2) = constant…………….(3)
Benzoic acid exit dimmer in benzene solution. Thus, distribution low can be used for ascertaining
molecular association.
From equation (3) we get,
C1/(n√C2) = K constant
logC1 = (1/n) logC2 + log K
nA - (A)n
C3 solvent 2 C2
Solvent 1
C1
No change
Log C1
Slope = 1/n
Log K
Log C2
When a solute is shaken with two immiscible solvents, it gets distributed between the solvents.
This distribution of solute in two solvents depends upon the solubility of the solute in two solvents.
At the distribution equilibrium, the ratio of concentration of the solute in the two solvents is
constant at a given temperature. The constant is called the partition co efficient (K) or the
distribution coefficient of the solute between two solvents.1
In the chemical and pharmaceutical sciences, both phases usually are solvents. Most commonly,
one of the solvents is water, while the second is hydrophobic, Hence the partition coefficient
measures how hydrophilic ("water-loving") or hydrophobic ("water-fearing") a chemical
substance is.2
Earlier The partition coefficient was known as distribution ratio.The first study of partition
coefficient was performed by Berthelotand Jungfleisch and came to result that the ratio of
concentration of solute is constant and doesnot depend on solvent volume.But Nernst have proved
that partition coefficient remains constant if single type of solute is used.It is the activity of solute
to enter in organic phase or aqueous phase which is explained by Partition Law by taking benzoic
acid in benzene and water,which explained that benzoic acid exist in dimeric form.3
Partition coefficients can be measured experimentally in various ways (by shake-flask, HPLC,
etc.) or estimated by calculation based on a variety of methods (fragment-based, atom-based, etc.).
Objective:
To determine the distribution coefficient of benzoic acid between benzene and water.
1
https://labmonk.com/partition-co-efficient-of-benzoic-acid-in-benzene-and-water
2
https://www.google.com/search?client=firefox-b-d&q=distribution+coefficient+of+benzoic+zcid
3
https://www.ripublication.com/ijcher16/ijcherv8n1_04.pdf?fbclid=IwAR0vaPIRWLUfWoJwBhPASP7DN89Lr2d-
sPHL92rnH6Gsv_h4chNF6S3T6Xw
Result and calculation:
Analysis of benzene layer:
Bottle no Volume of Average Conc. Of
Benzene Volume of 0.1M NaOH volume Benzene
layer, ml 1 2 3 layer, Cor
01 5 8.5 8.7 8.9 8.7 0.087
02 5 17.1 16.5 16 16.53 0.165
03 5 15.5 16.5 14.4 15.4 0.154
04 5 26.7 25.6 27 26.43 0.2643
Evaluation of Cor/Caq :
Bottle no. Conc. Of Log Cor Conc. Of Log Caq Cor/Caq
Organic aqueous
layer Cor layer Caq
01 0.087 -1.06048 .00535 -2.27165 16.26168
02 0.165 -0.78252 .01 -2 16.5
03 0.154 -0.81248 .00798 -2.098 19.29825
04 0.2643 -0.5779 .01236 -1.90798 21.3835
A plot of log Caq against log Cor is given below:
0
-1.2 -1 -0.8 -0.6 -0.4 -0.2 0
-0.5
y = 0.7677x - 1.4488 -1
Log Caq
-1.5
-2
-2.5
Log Cor
Result:
1. The distribution coefficient of Benzoic Acid between two solvents Benzene and Water is:
K =2.34 × 10-2
2. So, it can be said that the benzoic acid is diereses in Benzene.
Discussion:
The experiment was carried out to study the distribution of benzoic acid between benzene and
water. The value of constant K and n was determined by the experiment. Four samples of benzoic
acid; 1,2,3 and 4gm were taken in 4 individual bottles. Each of which was carried under the
experiment to obtain concentrations of benzoic acid in water (Caq) and pure benzene (Corg). the
ratios of (Corg/Caq ) for 4 samples of 1,2,3, and 4 gm benzoic acid, evaluated were 16.26168, 16.5,
19.29825 and 21.3835 respectively. A plot was established by placing log (Caq ) against log(Corg )
using the tabulated values. From the figure, it can be seen that the resultant curve is a straight line.
The slope of which is 1/n and Y-intercept is log(K). The value of n evaluated was 1.30 and that of
distribution coefficient K between benzene and water was 2.34 × 10 -2. This value actually shows
that benzoic acid undergone association in benzene solvent, consequently forming dimmer of
benzoic acid. The experiment was carried under the control of some significant conditions
(Constant temperature, Non-miscibility of solvents, same molecular state)