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AMINONIR®
Validation Results
Achieved N Goal
Parameters Conditions
% 40 %
DM <0.75 85 34 60
0.75-1.25 13 5 30
>1.25 3 1 10
CP <3.0 90 36 80
3-5 10 4 15
>5.0 0 0 5
AA <5 88 424 80
5.0 - 10.0 10 47 15
>10 2 9 5
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Agenda
Introduction
− AMINONIR®
− AMINOProx®
− AMINONRG®
Importance of grinding
Interpretation of Analytical Data
How to maximize the analytical data
Global Analysis Overview
Comparison of key raw materials
Conclusion
Your Challenges:
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Weighing Breed/Variety
Transportation Processing
Analytical/Human Storage
factor
Introduction:
- AMINONIR®
- AMINOProx®
- AMINONRG®
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Reflection
Detector
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AMINOLab®:
The most reliable reference for amino acid analysis
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AMINOLab®
Since 1960 Evonik (former Degussa) supports their customers in the feed
industry with AMINOLab® and AMINOBatch® services for raw material
selection and procurement, quality control, feed formulation and feed mill
management.
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AMINOLab®
− 58 years of experience in wet chemistry amino
acids analysis
− Data basis of more than 100.000 analysed feed
ingredients
− Continuous participation at „ring tests“ to check
for the precision
and accuracy of our analytical methods (AAFCO,
BIPEA, VDLUFA)
− Confirmed performance according to stringent
standards leads to EC directives and official US
methods:
• Total content after Oxidation • AOAC 994.12 (1994), EU Commission
and Hydrolysis (Directive 1998, Regulation 2009)
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AMINONIR®
Prediction Accuracy
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NIR-value (%)
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Crude protein
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Argentina
Brazil
USA
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40 ideal line
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30 35 40 45 50 55
Lab-value (%)
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NIR-value (%)
Argentina Methionine
Brazil
USA
ideal line
Lab-value (%)
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21 Corn by-products (corn bran, corn germ, corn germ meal (de-oiled) , corn gluten feed, corn
hominy feed, corn feed flour)
AMINONIR®
Methionine Glycine
Cystine Serine
Methionine + Cystine Alanine
Lysine Aspartic Acid
Arginine Glutamic Acid
Isoleucine
Threonine Ammonia (NH3)
Tryptophan Total Amino Acids with NH3
Leucine Total Amino Acids without
Valine NH3
Histidine
Phenylalanine Dry Matter
Crude Protein
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Proximate analysis
(Weende Analysis)
Phosphorus
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AMINOProx® calibrations
− Dry Matter
− Crude Ash
− Crude Protein
− Crude Fat
− Sugars
− Starch
− Crude Fibre
− Neutral Detergent Fibre
− Acid Detergent Fibre
− Phosphorus
− Phytic Phosphorus
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ICP
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AMINONRG® *:
Swine:
Gross Energy (GE) – Ewan, 1989
Digestible Energy (DE) – Noblet & Perez, 1993
Metabolizable Energy (ME) GP – NRC, 2012
Metabolizable Energy (ME) S – INRA, 2004
Net Energy (NE) – Noblet et al., 1994
Poultry:
Apparent Metabolizable Energy (AMEn) – WPSA, 1989
*AMINONRG® estimates the energy (GE, DE, ME, NE and AMEn) contents of different Feed Raw materials by
using selected regression equations that link the proximate composition predicted by AMINOProx® via Near
Infrared (NIR) spectroscopy.
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• Version
• Products
• Number of Calibration
Samples
• Collection Time of
Calibration Samples
• Origin of Calibration
Samples
• Calibration Parameters
• Calibration Statistics
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Europe
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867 NIR-Spectrometers
use
AMINONIR® calibrations
(January 2018)
Brazil MEA
81 58
ASIA South
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Spanish-
speaking
America
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AMINONIR® Advanced
Registration of the
Validation of the Upload spectra files
customer instrument
customer’s of a single ingredient
in Evonik’s NIR
instrument in Evonik portal
network
Results can be
Start spectra
downloaded in
processing
Evonik portal
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AMINONIR® Advanced
Application
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AMINONIR® Advanced
- Select spectra files
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AMINONIR® Advanced
- Upload spectra files
Either Foss or
Bruker spectra
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AMINONIR® Advanced
- Check suggestions
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AMINONIR® Advanced
- Start analysis
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AMINONIR® Advanced
- Spectra are processed
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Analytical Data
Advanced 3.0
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Download report
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Influence of
Grinding on NIR
prediction accuracy
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• AMINONIR® is a very quick and accurate method to analyse feed raw materials.
• Compared to wet chemistry the procedure of AMINONIR® is more simple.
• Correct grinding is the only sample preparation needed.
• Because a capable grinder is essential and grinding is time consuming the
importance of grinding often is put into question.
• To quantify and visualize the influence of grinding on NIR prediction accuracy an
extensive grinding test was performed covering all current AMINONIR®
calibrations.
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AMINONIR®
recommended
grinding
Different grinding procedures for a Sunflower Meal
unground Coffee grinder, 15 sec Coffee grinder, 60 sec Retsch ZM200, 1 mm Retsch ZM200, 0.5 mm
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Coffee grinder 15
Raw material Samples Retsch 1 mm Coffee grinder 60 sec
sec
Barley 5 3 5
Corn 5 1 3
DDGS, Non-corn Cereals 5 2 2 2
DDGS, Corn 5 1
Fish Meal 5
Meat Meal 5 1
Peas 2 2 2
Rapeseed Meal and Expeller 10
Rice Bran 5
Rye 5 1 5 5
Sorghum 5 2 5
Soybean Meal and Full Fat 10
Sunflower Meal 5 1 2
Triticale 1 1
Wheat 10 3 10
Wheat Bran 2
Coconut Meal (Copra) 5 5 5 5
Total 90 9 24 41
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AMINONIR®
• Capable instrument
• Capable grinder
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Grinding tips:
Recommended rotor speed for feed samples: (Retsch ZM200)
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• Customer
• Sample description
• Lab code
Raw Material by Wet Chem • Date of delivery / Date of
or AMINONIR® release
• Crude Protein – standardized /
as is
• Dry matter
• Amino Acids total contents -
standardized / in CP / as is
• Sum Amino Acids (without
NH3)
• Ammonia (NH3)
Dry Matter standardized
• Total (including NH3)
information
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• Data for amino acids and crude protein contents are expressed on a
dry matter basis to eliminate variations in the analytical results due to
moisture
• Calculation: AAASIS
AADMS = _________ * DMSFeedIngredient
DMASIS
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• Customer
• Sample description
• Lab code
Raw Material by Wet Chem • Date of delivery / Date of
or AMINONIR® release
• Crude Protein – standardized /
as is
• Dry matter
• Amino Acids total contents -
standardized / in CP / as is
• Sum Amino Acids (without
NH3)
• Ammonia (NH3)
• Total (including NH3)
Crude Protein based
information
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• This method does not distinguish between protein nitrogen and non-
protein nitrogen
• The CP content is calculated: CP = nitrogen content (%) x CP factor
• CP Factors:
− 6.25 for typical feed ingredients and mixed feeds
− 6.37 for dairy products
− 5.68 for collagen, gelatin
• This number is derived from the fact that the average nitrogen content
of proteins is 16%, thus 6.25 is the reciprocal of 0.16 or 16%
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• The sum of the analyzed amino acids (wet chemistry: essential and non
essential) is not 100%, compared to crude protein, but mostly 10% or
more below
• Calculation: AAASIS
AAin CP = _________ * 100
CPASIS
• The total amino acid to CP ratio is quite constant within a feedstuff type
• The CP content should not be used as the only criteria for quality
control
• The nitrogen content has its origin not only in amino acids, but also in
non-protein-nitrogen sources (NPN) potentially added to pure protein
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medium quality
What do we do with the information
from the Analytical Data?
% Lysine
Evaluation
0,60 0,70 0,80 0,90 1,00 Formulation
1,10 1,20
of Origin
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Recommendations:
• Increase the number of spectra uploads based on the historical observed variation per raw material
• Identification of additional support from Evonik (sampling, data analysis, raw material reporting, etc.)
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Impact of different
SBM Qualities on
Diet Formulation
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Soybean meal 1241 Mean 46.47 2.81 0.62 0.66 1.28 1.80 0.62 3.38 2.11 3.53 2.20 1.21 2.36
Mean 47.26 2.92 0.63 0.67 1.30 1.84 0.63 3.48 2.18 3.62 2.26 1.24 2.43
SD 1.50 0.09 0.02 0.03 0.04 0.05 0.02 0.11 0.08 0.11 0.07 0.03 0.09
Soybean meal 8246 CV 3.17 3.15 3.16 3.80 3.40 2.64 3.13 3.28 3.63 3.08 3.05 2.79 3.72
Min 34.17 2.17 0.47 0.49 1.01 1.34 0.46 2.55 1.56 2.59 1.62 0.90 1.73
Max 51.49 3.23 0.68 0.73 1.41 1.97 0.70 3.86 2.49 3.99 2.49 1.35 2.82
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Cost per kg: IDR 4,897 Cost per kg: IDR 4867
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Origin A Origin B
71 Evonik - Animal Nutrition
Origin A Origin B
72 Evonik - Animal Nutrition
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*Assumption: Wheat 168.00 €/ton; SBM 354.00 €/ton; Corn 164.00 €/ton; Soybean oil 761€/ton;
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QC sampling program:
Corn
Soybean meal
Ricebran
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Analytical costs:
If analyzing for crude protein and then using regression equations for amino
acid values:
$280,000/year
If analyzing via wet chemistry:
$1.05 million/year
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AMINONIR®
Allocation of samples 2017
Brazil
USA
Brazil
India
China
Mexico
Ecuador USA Pakistan
Chile South Africa
Ukraine
Argentina
Bolivia
Indonesia
Russia
Jamaica
Australia
Germany India Germany
Australia Russia
Bolivia
Jamaica China Ukraine
Indonesia Chile
Ecuador
Argentina Spain
Philippines
Mexico Peru
South Africa Mexico Hungary
Pakistan Vietnam
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AMINONIR® Advanced
Online NIR prediction via Internet
900000
850000 AMINONIR®-Spectra
800000 (predicted manually in Hanau)
750000
700000
650000 AMINONIR®-Advanced
600000 (fully automated application)
550000
500000
450000
400000
350000
300000
250000
200000
150000
100000
50000
0
2006 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017
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20000
15000
10000 9202
8507 8287 8191
6576
5908
4969 4880 4724
5000 4142 4025 3681 3674
3652 3630 3584 3484 3343
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Spectra information
Sample name
Sample code
Country of Origin (Imported or Local)
Supplier (trader)
Delivery date
Truck number
Analyst
P/O Number
Lot no.
…..
Information required varies, depending on what is important to the customer.
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• Air-conditioned lab
• Experienced and reliable person who maintains
the NIR instrument and runs the analytical service
• Retsch Grinder ZM 200 (0.5 mm sieve)
• Suitable NIR instrument
• NIR instrument connected with internet
• Ongoing assessment of the analytical results
according supplier, origin or processing plant
• Standardization of the customer instrument will be
done by one of our regional NIR experts
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Mean 8.25 0.25 0.17 0.18 0.36 0.30 0.06 0.40 0.28 0.97 0.39 0.24 0.40
India 9418
CV 6.15 5.67 6.40 4.92 5.20 6.05 4.57 5.79 6.85 8.32 5.87 6.14 7.52
Mean 7.52 0.24 0.15 0.17 0.32 0.27 0.06 0.36 0.36 0.91 0.36 0.22 0.37
USA 475
CV 7.31 4.17 6.67 5.88 6.25 7.41 4.71 5.56 7.69 8.79 5.56 4.55 8.11
Mean 7.80 0.23 0.16 0.18 0.34 0.28 0.06 0.37 0.27 0.95 0.37 0.23 0.38
Brazil 9515
CV 4.61 5.10 5.79 3.99 4.35 4.51 3.71 4.90 5.25 6.68 4.47 4.56 5.89
Mean 7.21 0.24 0.14 0.16 0.30 0.26 0.06 0.37 0.24 0.81 0.34 0.21 0.33
Argentina 294
CV 8.30 9.70 7.30 8.10 6.70 6.00 8.60 11.80 14.80 9.80 10.20 13.50 8.30
Poultry SIDC 91 95 89 92 89 83 89 98 93 95 97 93
Swine SIDC 75 87 83 85 80 77 89 86 89 85 87 87
90
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Mean 47.21 2.92 0.63 0.67 1.30 1.84 0.64 3.46 2.18 3.61 2.26 1.24 2.42
SEA 14237
CV 2.90 3.13 2.72 3.58 3.02 2.43 2.88 3.21 3.49 2.93 2.88 2.59 3.58
Mean 47.14 2.88 0.61 0.64 1.26 1.80 0.62 3.48 2.16 3.58 2.22 1.23 2.41
India 10686
CV 1.72 2.35 2.45 4.92 2.70 2.22 2.57 2.14 2.17 2.09 2.17 2.26 2.08
Mean 46.12 2.87 0.62 0.67 1.30 1.79 0.64 3.38 2.11 3.50 2.21 1.20 2.32
USA 253
CV 1.41 1.39 1.61 1.49 1.54 1.12 1.56 1.48 1.42 1.43 1.36 1.67 1.72
Brazil Mean 46.50 2.87 0.62 0.67 1.28 1.81 0.63 3.39 2.14 3.56 2.22 1.21 2.38
(46-47% 8814
CP) CV 0.60 1.89 1.57 1.93 1.63 1.27 1.34 1.23 0.92 1.14 0.96 1.47 1.12
Mean 45.38 2.79 0.60 0.64 1.25 1.77 0.61 3.28 2.08 3.47 2.18 1.19 2.30
Argentina 158
CV 1.60 2.00 2.20 2.80 2.80 1.60 1.80 1.80 2.00 1.70 1.80 1.80 1.90
Poultry SIDC 89 90 79 84 83 89 92 87 88 87 90 89
Swine SIDC 90 92 85 88 88 89 95 90 89 90 91 90
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2696 1279
2308
8342
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Starch Sugar
0.8 0.71 0.74 0.69 12
0.6 10.03 9.95
10 8.82
0.6
8 6.79
0.4 6
0.2 4
0 2
0
Starch Sugar
Crop report 2017
0.76 9.07
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Total AA
USA 1279 2.90 0.63 0.68 1.83 0.64 3.43 2.14 2.24
Argentina 2308 2.88 0.62 0.66 1.83 0.63 3.41 2.14 2.24
India 8342 2.89 0.61 0.65 1.82 0.63 3.52 2.17 2.24
Brazil 2696 2.97 0.64 0.69 1.88 0.65 3.55 2.24 2.32
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Conclusions
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