TAT BROTH

INTENDED USE
Remel TAT Broth is a liquid medium recommended for use in qualitative procedures for sterility testing of cosmetics, topical drugs, and highly
viscous substances, such as salves and ointments.

SUMMARY AND EXPLANATION

TAT Broth is also referred to as Fluid Casein Digest-Soy Lecithin Polysorbate 20 Medium. It is prepared according to the formulation
1
recommended by the Food and Drug Administration. TAT Broth is a noninhibitory medium, used primarily for isolating contaminating bacteria
2
in cosmetics, salves, and creams. In 1976, Brummer reported lecithin and polysorbate 20 effectively neutralized chemical disinfectant
3
residues which were inhibitory to the growth of viable microorganisms. TAT Broth is recommended by the United States Pharmacopeia for
4
use in microbiological examination of non-sterile products.

PRINCIPLE
Casein peptone provides a source of nitrogen, amino acids, and peptides essential for the growth of bacteria. Lecithin and polysorbate 20
serve to neutralize preservatives in topical drugs and cosmetics.

REAGENTS (CLASSICAL FORMULA)*

Casein Peptone................................................................. 20.0 g Polysorbate 20.................................................................40.0 ml
Lecithin................................................................................ 5.0 g Demineralized Water .....................................................960.0 ml
pH 7.2 ± 0.2 @ 25°C
*Adjusted as required to meet performance standards.

PRECAUTIONS
This product is For Laboratory Use only. It is not intended for use in the diagnosis of disease or other conditions.

PREPARATION OF DEHYDRATED CULTURE MEDIUM

1. Suspend 25 g of medium in 960 ml of demineralized water.
2. Add 40 ml of polysorbate 20.
3. Heat to 50-60°C and let stand for 15-30 minutes with occasional agitation to completely dissolve.
4. Sterilize by autoclaving at 121°C for 15 minutes or following established laboratory procedures.
5. Dispense into appropriate containers.

PROCEDURE
1. Consult current editions of appropriate references for the recommended procedure for sample preparation, inoculation, testing, and
interpretation.

QUALITY CONTROL
Each lot number of TAT Broth has been manufactured, packaged, and processed in accordance with current Good Manufacturing Practice
regulations. All lot numbers have been tested using the following quality control organisms and have been found to be acceptable. Testing of
control organisms should be performed in accordance with established laboratory quality control procedures. If aberrant quality control results
are noted, sample results should not be reported.

CONTROL INCUBATION RESULTS

®
Bacillus subtilis ATCC 6633 Aerobic, 18-24 h @ 33-37°C Growth
®
Escherichia coli ATCC 25922 Aerobic, 18-24 h @ 33-37°C Growth
®
Pseudomonas aeruginosa ATCC 27853 Aerobic, 18-24 h @ 33-37°C Growth
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Staphylococcus aureus ATCC 25923 Aerobic, 18-24 h @ 33-37°C Growth

BIBLIOGRAPHY
1. Food and Drug Administration. 1969. Procedure for Examination of Topical Drugs and Cosmetics. FDA, Rockville, MD.
2. MacFaddin, J.F. 1985. Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. 1. Williams & Wilkins, Baltimore, MD.
3. Brummer, B. 1976. Appl. Environ. Microbiol. 32:80-84.
4. The United States Pharmacopeia. 2007. 31st ed. United States Pharmacopeial Convention, Rockville, MD.

Refer to the front of Remel Technical Manual of Microbiological Media for General Information regarding precautions, product storage and
deterioration, sample collection, storage and transportation, materials required, quality control, and limitations.

ATCC® is a registered trademark of American Type Culture Collection.

IFU 112611, Revised March 24, 2010 Printed in U.S.A.

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