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1
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, India.
2
GITAM University – Bengaluru, Karnataka-562163, India.
3
Department of Science and Humanities, Vignan’s Foundation for Science,
Technology and Research, Vadlamudi-522213, India.
*Corresponding athor E-mail: doctornadh@yahoo.co.in
http://dx.doi.org/10.13005/ojc/35Specialissue107
ABSTRACT
A simple spectrophotometric method was developed to determine riociguat in bulk and tablet
formulation. The present method lies on the oxidation of MBTH by Fe+3 ions in acidic medium to form
active coupling species and followed by its coupling with riociguat to form the chromophore having
lmax 660 nm. Validated the proposed method as per the existing guidelines of ICH. Good linearity (r~
0.999) was observed for calibration curve in the studied concentration range (6.25 – 37.50 μg mL-1).
Reproducibility, accuracy and precision of the method were confirmed from low values of % RSD.
This is an Open Access article licensed under a Creative Commons license: Attribution 4.0 International (CC- BY).
Published by Oriental Scientific Publishing Company © 2018
Ratnakaram et al., Orient. J. Chem., Vol. 35(Special Issue 1), 48-53 (2019) 49
the plausible electrophilic substitution is shown in the accuracy of the method from lower values of
Figure 4. %RSD as well as S.D.
S -2e S S
N NH2 N N
N -H+ N NH
N NH
CH3 CH3 CH3
MBTH (I) Electrophile ( II )
S N N N
N S
N + NF N NF
N NH N N
CH3 CH3
N N N N
O O
Riociguat Green coloured complex
Fig. 5. Calibration graph of Riociguat
Conclusion Acknowledgement
The proposed method is the first report Authors are thankful to Acharya Nagarjuna
on the visible spectrophotometric determination of University and GITAM-Bengaluru Campus for
riociguat (bulk drug and pharmaceutical dosage providing facilities to carry out the present research
forms). In addition, it can be used in routine analysis work.
as an alternative to the expensive instrumental
methods. Conflict of interest: NIL
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