Chapter III

METHODOLOGY

This chapter contains the locale of the study, the sampling procedures including the
collection of top 3 leading energy drink brands samples and preparation of the composite sample,
and the statistical tool that was used in this study.

Locale and Subject of the Study

This study will be conducted in Father Saturnino Urios University Morelos Campus
where Energy Drinks consumed by the Student-Athletes of FSUU. The campus of Father
Saturnino Urios University is located at Libertad, Butuan City.

This study will focus more on the concentration found in tartrazine. The researchers will
collect 2 samples in each energy drinks (Gatorade, cobra & sting) to know and determine if there
is signifant difference among these three. The abundance of energy drinks that will be collected
will be considered as the vital part of our study.

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Map of Agusan del Norte Map of Philippines

Map of FSUU
 Sampling Procedures

Collection on Energy Drinks

The energy drink samples will be randomly collected within the area of Butuan City,
Agusan Del Norte. There will be three energy drinks which is the gatorade, cobra and sting to be
prepared which will indicate to determine if there is significant difference among these three (3)
top leading brands. The researchers will credit the three brands in which will be purchase on the
selected store in Butuan City. Each energy drinks samples will have different volumes. The
samples will be taken to MSU-IIT to test the concentration.

Preparation of Samples

The tartrazine of top three energy drinks will be find in the collected bottles, after that
they will remove or take the samples in a small bottle for the test and will transport it or bring it
to the testing center or laboratory which will be in Iligan City.

Statistical Analysis

The one-way analysis of variance (ANOVA) is use to determine whether there are any
statistically significant differences between the means of two or more independent groups. This
compares the mean concentration of tartrazine in the top 3 leading brands of energy drinks.