KM VALUE COMMENTA

SUBSTRATE ORGANISM UNIPROT

[mM] RY hide
pH and
temperature
0.00012 (S)-malate Escherichia - not
hide coli specified in
the
publication
Clonorchis
0.056 - pH 9.6, 25°C
sinensis
at 0.08-0.18
mM, pH and
temperature
Pseudomon -
0.063 not
as stutzeri specified in
the
publication
pH 8.0,
temperature
not
specified in
Taenia the
0.1 F1C7I4;
solium publication;
pH 9.6,
22°C,
recombinan
t enzyme
recombinan
Methylomic t enzyme,
0.11 robium G4T3S9; pH 10.0,
alcaliphilum 30°C
pH and
temperature
Aeropyrum - not
0.12 pernix specified in
the
publication
pH and
temperature
Helicobacter - not
0.177 pylori specified in
the
publication
Malus pH 7.5,
0.19 A3DSX0; 30°C; pH
domestica
7.9, 25°C
Taenia
0.215 - -
solium
pH and
temperature
Bacillus not
0.26 -
subtilis specified in
the
publication
 Flavobacteri 20°C, pH
0.269 um - 10.0
frigidimaris
30°C, pH
0.288 - 10.0
0.3 Sus scrofa - pH 8.1, 22°C
pH and
Methanothe temperature
rmobacter - not
0.4 thermautotr specified in
ophicus the
publication
pH and
temperature
Streptomyce - not
0.49 s coelicolor specified in
the
publication
0.494 Q9K3J3; pH 8.5, 30°C
pH 9.0,
Rhodopseud 25°C,
0.53 omonas - dimeric
palustris enzyme
form
Flavobacteri 10°C, pH
0.543 um - 10.0
frigidimaris
pH and
temperature
Glaesserella - not
0.55 parasuis specified in
the
publication
pH 9.0,
Rhodopseud 25°C,
0.555 omonas - tetrameric
palustris enzyme
form
pH 7.5,
Trypanosom O15769; 37°C,
0.56 a brucei mitochondri
al enzyme
recombinan
t wild type
Phytophthor -
0.64 mitochondri
a infestans al MDH, at
25°C

Macromona -
0.645 pH 9.0, 25°C
s bipunctata
 at 0.18-10.0
mM, pH and
temperature
Pseudomon -
0.67 not
as stutzeri specified in
the
publication
Flavobacteri 40°C, pH
0.74 um - 10.0
frigidimaris

native wild
type
Phytophthor -
0.94 mitochondri
a infestans al MDH, at
25°C

Leishmania - glycosomal
1.007 major isoenzyme
recombinan
t enzyme,
pH and
Mycobacteri temperature
1.04 um P9WK13; not
tuberculosis specified in
the
publication

Methylosinu recombinan
s
1.28 trichosporiu - t enzyme,
pH 9.5, 30°C
m

Vulcanither
mus
1.67 mediatlantic - pH 9.0, 25°C
us

recombinan
t wild type
Phytophthor -
1.72 cytosolic
a infestans MDH, at
25°C
pH 7.5,
Trypanosom O15769; 37°C,
2.72 a brucei glycosomal
enzyme
Leishmania - cytosolic
3.3 major isoenzyme
pH and
temperature
uncultured - not
4 bacterium specified in
the
publication
 pH and
temperature
Nitrosomon - not
5 as europaea specified in
the
publication
pH and
Kitasatospor temperature
a not
9 aureofacien - specified in
s the
publication
mitochondri
Leishmania -
15 al
major isoenzyme
LITERATURE

740926

668523

740926

722201

740975

740926

740926

712865

700562

740926
668012

668012

712915

740926

740926

711519

667752

668012

740926

667752

669002

700338

667240
740926

668012

700338

670193

741234

740975

667240

700338

669002

670193

740926
740926

740926

670193
TURNOVER
NUMBER SUBSTRATE ORGANISM UNIPROT
[1/s]

4.71 (S)-malate Streptomyce Q9K3J3;

hide s coelicolor
Malus
8.4 A3DSX0;
domestica
9.91 A3DSX0;
18 Sus scrofa -
Flavobacteri
41.3 um -
frigidimaris

Taenia
47 F1C7I4;
solium

47.4 F1C7I4;

Flavobacteri
48.4 um -
frigidimaris

83.2 -

138 -

Mycobacteri
302 um P9WK13;
tuberculosis

Methylomic
621 robium G4T3S9;
alcaliphilum

Methylosinu
s
1141 trichosporiu -
m
COMMENTA LITERATURE
RY hide

pH 8.5, 30°C 711519

pH 7.9, 25°C 712865

pH 7.5, 30°C 712865

pH 8.1, 22°C 712915

10°C, pH 668012
10.0
pH 9.6,
22°C, 722201
recombinan
t enzyme
pH 8.0,
temperature
not 722201
specified in
the
publication

20°C, pH 668012
10.0

30°C, pH 668012
10.0
40°C, pH 668012
10.0
recombinan
t enzyme,
pH and
temperature 741234
not
specified in
the
publication

recombinan
t enzyme, 740975
pH 9.5, 30°C

recombinan
t enzyme, 740975
pH 9.5, 30°C
kcat/KM COMMENTA
VALUE SUBSTRATE ORGANISM UNIPROT RY hide
[1/mMs-1]

recombinan
t enzyme,
pH and
Mycobacteri
0.29 (S)-malate temperature
um P9WK13;
hide not
tuberculosis specified in
the
publication
Streptomyce Q9K3J3;
9.53 pH 8.5, 30°C
s coelicolor
pH 7.5,
Malus
44.26 A3DSX0; 30°C; pH
domestica 7.9, 25°C
Methylosinu recombinan
s
403 trichosporiu - t enzyme,
pH 9.5, 30°C
m
pH 8.0,
temperature
not
specified in
Taenia the
474 F1C7I4;
solium publication;
pH 9.6,
22°C,
recombinan
t enzyme
Methylomic recombinan
5648 robium G4T3S9; t enzyme,
alcaliphilum pH 9.5, 30°C
LITERATURE

741234

711519

712865

740975

722201

740975