DETAILED BIO-DATA

1. Name of the Applicant: Dr Winlet Sheba Shadrach

2. Mailing Address
D-4/4068, Vasant Kunj
New Delhi 110070
011-26123245 (Phone)
011-26125337 (Fax)
shebadavidson@yahoo.com

3. Date of Birth: 16 April 1972

4. Educational Qualifications (Starting from

Graduation onwards):

S.No. Degree University Year Subjects Percentage

1 B.Sc (Hons.) University 1992 Microbiology, 70.82
Microbiology of Delhi Biochemistry,
Biophysics,
Biomathematics,
Genetics,
Immunology
2 M.Sc Microbiology University 1994 Microbiology, 75.90
of Delhi Biochemistry,
Biophysics, Genetics

3 PhD Microbiology University 1998 Microbial lipases

of Delhi

5A. Details of professional training and research experience, specifying period.

I have a considerable amount of research experience starting with my M.Sc

dissertation work. I have acquired skills and expertise in the areas of microbial
fermentation, enzyme purification and crystallisation, biochemical characterisation of
proteins, and enzyme-mediated reactions in aqueous and non-aqueous media.

I have worked both in academic and industrial environments and made significant
contributions in India, the United Kingdom and Germany.

B. Details of employment

a) Worked as a Post-doctoral Scientist at the Robert Koch Institute, Nordufer

20, D-13353 Berlin, Germany (January - December 2003).
 Nature of duties included: the one-year project entitled "Biochemical
characterisation of the phospholipases A of amoebae as natural hosts of the
bacterium Legionella" involved work on the following 5 objectives:

1. Investigation of the secretion and intracellular accumulation kinetics of

phospholipase A-activity (-ies) of/in Acanthamoeba castellanii and its
(their) substrate specificity.
2. Partial biochemical protein purification of the phospholipase A-activity
(-ies) of A.castellanii culture supernatants/cell lysates, classification of
the activities according to their substrate specificity, and approximate
determination of their molecular weight.
3. Determination of phospholipase A-activity compared to uninfected
amoebae at several time points Acanthamoeba castellanii infection
with Legionella pneumophila.
4. Investigation of intracellular multiplication of Legionella pneumophila
in Balamuthia mandrillaris.
5. Analysis of Balamuthia mandrillaris culture supernatants/cell lysates
for phospholipase A-activity.

b) Worked as Advanced Development Scientist in the Inhalation Drug

Delivery division of 3M Healthcare Limited, Ashby Road site, 3M House,
Morley Street, Loughborough, UK (July – December 2000).

Nature of duties included: biochemical work on deoxyribonuclease I

(DNase I), an enzyme for the treatment of cystic fibrosis in human beings;
development/validation of assay methods; purification and
characterization; formulation of the enzyme in metered dose inhalers and
studies on the stability of the formulations.

c) Worked in a supervisory role in the Quality Assurance department of a

sandwich-filling-producing food production plant, M/s. FreshPak Chilled
Foods Limited, Kernan Drive, Belton Industrial Estate, Loughborough,
UK (February – May 2000).

Nature of duties included: Supervision of the maintenance of

microbiologically sterile conditions in the production plant, new product
development; and a member of the taste panel of the company.

d) Worked as a post-doctoral fellow in a project on the purification,

crystallisation and structure-solution studies of a phospholipase from the
venom of Red Scorpion under the supervision of Professor T.P.Singh at the
Department of Biophysics, All India Institute of Medical Sciences, New
Delhi (August – November 1999).

Nature of duties included: biochemical characterisation of the

phospholipase; purification of the enzyme; crystallisation; and testing of
the anti-microbial activities of some natural and synthetic peptides.
C. List of significant publications:

Patents filed for:

1. A process for the isolation of optically pure S-enantiomer of
cyanohydrin of meta-phenoxybenzaldehyde via lipase-mediated
enantioselective transesterification (Application no. 1378/Del/99).
2. A process for the preparation of lipase from the fungus, Aspergillus
carneus (Application no. 1379/Del/99).

Publications:

Published papers (maiden name:- Davidson)

1. Microbial lipases : production and applications. Ghosh, P.K., Saxena,

R.K., Gupta, R., Yadav, R.P. and Davidson, W.S. (1996). Sci. Prog.
79 : 119- 157.

2. Production of biosurfactant from sugar alcohols and natural

triglycerides by Aspergillus terreus lipase. Yadav, R.P., Saxena, R.K.,
Gupta, R. and Davidson, W.S. (1997). J. Sci. Indus. Res. 56 : 479-
482.

3. Utility of a novel lipase from Aspergillus terreus in deacetylation

reactions. Parmar, V.S., Pati, H.N., Yadav, R.P., Kumar, A., Bisht,
K.S., Gupta, R., Davidson, W.S., Poonam and Saxena, R.K. (1998).
Biocatal. Biotransform. 16 (1) : 17-25.

4. Lipase production by Aspergillus and Penicillium species. Yadav, R.P.,

Saxena, R.K., Gupta, R. and Davidson, W.S. (1998) Folia Microbiol.
43 (4) : 373 - 378.

5. Rapid zymogram for lipase. Yadav, R.P., Saxena, R.K., Gupta, R. and
Davidson, W.S. (1998). BioTechniques 24 (5) : 754 - 756.

6. Potential use of a novel lipase from Aspergillus carneus in

deacetylation reactions. Parmar, V. S., Kumar, A., Poonam, Pati,
H.N., Saxena, R.K., Davidson, W.S. and Gupta, R. (1998). Biochim.
Biophys. Acta 1387 : 325-330.

7. Purification and characterization of a regiospecific lipase from

Aspergillus terreus. Yadav, R.P., Saxena, R.K., Gupta, R., and
Davidson, W.S. (1998). Biotechnol. Appl. Biochem. 28(3) : 243-249.

8. The fungistatic action of oleic acid. Davidson, W.S., Saxena, R.K. and
Gupta, R. (1999). Curr. Sci. 76 (8) : 1137-1139.

9. Microbial enzymes for detergent application. Saxena, R. K.,

Davidson, W.S. and Gupta, R. (1999). In : Advances in microbial
biotechnology (eds. Tiwari, J. P., Lakhanpal, T. N., Singh, J., Gupta,
 R. and Chamola, B.P.) Aditya Publishing House, New Delhi. pp. 77-
91.

10. Microbial lipases : potential biocatalysts for the future industry.

Saxena, R.K., Ghosh, P.K., Gupta, R., Davidson, W. S., Bradoo, S.
and Gulati, R. (1999) Curr. Sci. 77(1) : 101-115.

11. Parametric optimisation of Aspergillus terreus lipase production and its

potential in ester synthesis. Gulati, R., Saxena, R.K., Gupta, R., Yadav,
R.P. and Davidson, W.S. (1999). Process Biochem. 35(5) : 459-464.

12. Purification strategies for microbial lipases. Saxena, R.K.,Sheoran, A.,

Giri, B. and Davidson, W.S. (2003) J. Microbiol. Methods. 52 : 1-18.

13. Purification and characterization of an alkaline thermostable lipase

from Aspergillus carneus. Saxena, R.K., Davidson, W.S., Sheoran, A.
and Giri, B. (2003) Process Biochem. 39(2) : 239 - 247.

14. Pathogenese der Legionelleninfektion. Flieger, A., Banerji, S., Broich,

M., Rydzewski, K. and Shadrach, W.S. (2003) Bundesgesundheitsbl -
Gesundheitsforsch - Gesundheitsschutz 46 : 694 - 699.

15. The pathogenic amoeba Balamuthia mandrillaris possesses cell-

associated phospholipase A, lysophospholipase A and lipase activities.
Shadrach, W.S., Radam, E., Flieger, A. and Kiderlen, A.F. (2004)
Int J Med Microbiol 293(suppl 38):100. 21. Jahrestagung der
Deutschen Gesellschaft für Parasitologie. 17.-20.03.2004, Würzburg,
Germany.

16. The pathogenic amoeba Balamuthia mandrillaris is a host for

intracellular multiplication of Legionella pneumophila bacteria.
Shadrach, W. S., Laube, U., Holland, G., Özel, M., Kiderlen, A. F. and
Flieger, A. (2004) Int. J. Med. Microbiol. 293(Suppl. 38): 54.

17. Balamuthia mandrillaris, free-living amoeba and opportunistic agent

of encephalitis, is a potential host for Legionella pneumophila bacteria.
Shadrach, W. S., Rydzewski, K., Laube, U., Holland, G., Ozel, M.,
Kiderlen, A. F. and Flieger, A. (2005) Appl. Environ. Microbiol. , 71
(5) 2244-2249 .

General Articles

18. Review of book entitled 'Laboratory Manual in Microbiology' written

by P. Gunasekaran and published by New Age International (P) Ltd.,
Delhi. Saxena, R.K., Gupta, R.and Davidson, W. S. (1997).
Phytomorphology. 47 (3) : 343.

19. Poem entitled “The Incredible Aspergillus” Davidson, W.S., published

in “The Botanica” (The Delhi University Botanical Society,
 Department of Botany, University of Delhi, Delhi), Volume 49 (1999)
pp.76.

Presented 26 papers at various International and national conferences.

6. Professional recognition, awards, fellowships received

Awards

1. A.M.I. Prof. Rana Memorial Best Poster Award at the 35th Annual
Conference of the Association of Microbiologists (India) held at
Mysore in 1994.

2. Best Poster Presentation Award of Micon International - 94 held at

Mysore in 1994.

3. Best Poster Presentation Award at the 38th Annual Conference of the

Association of Microbiologists (India) held at New Delhi in 1997.

4. Best Poster Presentation Award at the National Symposium on “

Perspectives in interfacial areas of chemistry and biology” held at New
Delhi in 1998.

5. A.M.I. Young Scientist Award in Food and Fermentation Microbiology

at the 39th Annual Conference of the Association of Microbiologists
(India) held at Mangalore in 1998.

Scholarships

1. University of Delhi Postgraduate Scholarship from 1992 - 94.

2. University Grants Commission (India) - Junior Research Fellowship

from 1994 -96.

3. University Grants Commission (India) - Senior Research Fellowship

from 1996 -99.

Achievements

1. Gold medallist from the University of Delhi in 1994 in M.Sc.

Microbiology.
2. Second rank in the University of Delhi in 1992 in B.Sc. (Hons.)
Microbiology.