SCT60103 Genes and Tissue Culture Technology

Laboratory worksheet

Student name : Bilal Tahir

Student ID : 0328555

1. Record the number of viable and dead cells in the following table. [25 marks]

Table 1: Cell concentration at 5-day intervals

Number of hours (h)

24 48 72 96 120
8 34 41 44 100.25
1
5 24 41 43.75 77.25
2
Viable cells
15 19.5 32.75 43 177.25
3
9.33 25.83 38.25 43.58 118.25
Mean
7.46 x 104 2.07 x 105 3.06 x105 3.49 x 105 9.46 x 105
Total viable cell count
5 0.5 0.75 0.75 2.5
1
1 0.25 1.25 0.75 3.5
2
Dead cells
2 0.25 1 0.75 3.75
3
2.67 0.33 1 0.75 3.25
Mean
2.14 x 104 2.64 x 103 8 x 103 6 x 103 2.6 x 104
Total dead cell count
77.7% 98.6% 97.5% 98.3% 97.3%
Percentage cell viability
1.87 x 105 5.17 x 105 7.65 x 105 8.72 x 105 2.37 x 106
Cell concentration
9.6 x 104 2.10 x 105 3.14 x 105 3.55 x 105 9.72 x 105
Total cell count
SCT60103 Genes and Tissue Culture Technology

2. Plot the graph for cell concentration (cells/mL) on a log scale against number of hours.
[10 marks]

cell concentration (cells/ml)

2500000
cell concentration (cells/mL)

2000000

1500000

1000000

500000

0
0 20 40 60 80 100 120 140
Time (hrs)

Figure 1: graph for cell concentration (cells/mL) on a log scale against number of hours.