Mythic 22 Workbook-V3 PDF

Mythic™22
Service Engineer Training

Workbook
03/09/2009 -1- Orphée S.A/M22/SEW/003

Proprietary Statement
Copyright 2006, Orphée S.A. All rights reserved. No part of this document
may be reproduced, stored in a retrieval system or transmitted in any
form or by any means, electronic, mechanical, photocopied, recorded, or
otherwise, without the prior written permission of Orphée S.A.
Service engineer training workbook usage disclaimer
Information contained in this document was developed for use by trained

Orphée S.A. service engineers personnel, or by other persons
knowledgeable or experienced with operation and service of the Mythic
system.
Trademark statements
Mythic is a trademark of Orphée S.A.
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Revision history Pages revised and added
SEW/001 12/2006 First release
SEW/002 01/2008 Second release:

USB function version
2 and Higher
Cap Piercing version
SEW/003 09/2009 Third release:

Add electronic troubleshooting
Add Technical Notes in Section 8
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Mythic™22 Service Engineer Training Workbook
Index
Section 1……………………………Overview
Section 2…………………………..User Interface
Section 3…………………………..Mythic™22 Fluidic
Section 4…………………………..Mythic™22 Electronic
Section 5…………………………..Mythic™22 Mechanic
Section 6…………………………..Mythic™22 Optic
Section 7…………………………..Mythic™22 Heater
Section 8…………………………..Technical Notes
Section 9…………………………..Mythic™22 CT – Cap piercing module
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Section 1: Overview
 Learning Objectives
 Front view
 Right side view
 Rear view
 Left side view
 Physical specifications
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Learning objectives
Section 1 will provide and overview of the system and physical

dimensions of the Mythic™22.
After reviewing Section 1 of this workbook you should be able to do

the following:
 Identify the connections located on the rear of the

Mythic™22.
 Connect the right reagents on the right connectors.
 Identify the different fluidic module of the Mythic™22.
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Mythic™22 – front view
Power Switch
TFT Display
touchscreen
Numerical
Keyboard
Status Indicator
LED
Start Cycle key
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Mythic™22 – Right Side View
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Mythic™22 – Rear View
USB ports
TCP/IP port
Barcode
reader
Waste
connector RS232 port
Diluent
connector Centronic port
24 V Power
supply
External connector
grounding
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Mythic™22 – Left side view
Lyse solution
Straw
Cleaner Straw
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Mythic™22 – Physical Specifications
Physical dimensions
Dimension Instrument
Height 350 mm
Width 250 mm
Depth 340 mm
Weight 12 kg
Note: For complete listing of physical specifications, refer to

Mythic™22 System Operator’s Manual.
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Section 2: User Interface
 Learning Objectives.
 Operator Screens.
 Technician Screen.
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Learning Objectives
Section 2 will explain the user and service interface. After reviewing
this section of the workbook you should be able to do the following:
 List the main menus of the instrument.

 Describe how to calibrate the instruments.
 Describe how to restore Quality Control values.
 Describe how to connect the SVM.
 Describe how to check the pressure on the Mythic™22.
 Describe where to adjust the electronic gains.
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Operator Screens – Main tree
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Operator Screens – Login
1- Password:
 None : User – Lab tech

 123 : Chief of lab
 2TEC : Field Service Engineer
 MAPD : Super Technician
Note1: with MAPD password, everything is possible, no alert

message is displayed.
Note2: We recommend using the 2TEC password during the
installation of a new instrument.
2- Language:
Choose the selected language with the scroll bar.
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How to use the USB key
The USB key must be formatted before any use. The format should be done
on the Mythic 18.
How to proceed?
1. Plug the USB key on
o One of the two USB port for the Mythic 18 equipped with normal USB port.
o The USB port on the lower position for the Mythic 18 equipped with small
USB port (like camera USB port).
2. Go to Set Up, Advanced menu, Storage Options.
3. Then, press USB thumb drive format.
4. When the format is finished a prompt will appear.
o If the USB key is not compatible, a prompt will appear to inform you. In
this way, try another one.
NOTE: The format is done in FAT16. For this reason it is not necessary to
equip the Mythic 18 with a USB key with a capacity higher than 2 Gb.
2 Gb capacity = Around 60.000 patients.
128 Mb capacity = Around 10.000 patients.
Once your USB key is formatted, you can start to work with it.
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Overview of the main tree of the USB key:
The serial number of the Mythic 22 is coded as : xxxxxx-yyyyyy

These two groups of 6 digits will be used to create private folders, only usable on one
instrument.
Root folder for Mythic 22 analyser
Common folders. The content of this part

can be used on all Mythic 22 instruments.
NOTE: These folders must be created
manually, with Explorer Windows for
example.
All of these folders and datas contained in

these folders are dedicated to the
instrument and can’t be transfer to another
instrument.
Each folder is automatically created by the
Mythic 22.
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Folders description:
1/ Common datas
COMMONS DATAS : \ROOT\

(sub)Folder files contents comments Format and Analyser
unit Write/Read
LOT MXC1234.QCN Qc target values One file per target ASCII
MBC02174.QDL Limited at 100 files STD unit
(target name.Test_Level) Calibration target NO / YES
CAL0233.CAL values
(target name.CAL)
SETTINGS USER.SET Common settings One file ASCII + UTF-8 YES / YES
UPDATE\ V100-004.b Software release One file per software release binary NO/YES
( version name.b)
VERSION
UPDATE\ HP6122.LPT Printer drivers One file per printer driver binary NO/YES
(prn_name.port LPT or USB )
PRTDRV
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2/ Mythic 18 dedicated data
PRIVATES DATAS : \ROOT\xxxxxx\yyyyyy\

(sub)Folder Files name (example) contents comments Format and Analyser
unit Write / Read
CALI 221006.CAL Calibration report = One file per calibration date ASCII + UTF-8
(calibration date as calibration factors + current unit YES / NO
DDMMYY.CAL) target + results
LOG LOG_ERR.CSV Error log Two files ASCII YES / NO
LOG.CSV service log
QC CRXV6875.QCN QC report = One file per QC target and level ASCII + UTF-8
(target name.level) target + results STD unit YES / YES
RESULTS\ 0047.CSV Result analysis One sub folder per Year/month (YYYY- ASCII + UTF-8
(sequence number. CSV) MM), current unit
YYYY-MM\
One sub folder per day (DD), YES / YES
DD One file per result
Limited to 510 files
SETTINGS TECH.SET Private analyzer settings One file ASCII + UTF-8
YES / YES
REAGENTS REAGLOGS.CSV Private reagents log One file ASCII
YES / YES
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Once installed, you can use the USB key how you want.
Two cases:
5. The USB key is not connected on the instrument:
All the patient results will be saved on the internal memory of the instrument.
NOTE: The capacity of the internal memory is 1.500 patient results instead of
500 in the previous version.
When the key will be locked on the instrument, the Mythic 18 will ask you if
you want to transfer the data on the USB. In this way, these data will be
erased from the internal memory.
6. The USB key is connected on the instrument:
If you have activated the option Real time runs save in the Storage Options,
all the results from the patient will be directly saved on the USB key.
NOTE: To avoid the problem of memory full, we recommend activating the

option FIFO Mode (First In, First Out) in the storage options. When the
memory is almost full the first result will be erased and replaced by the last
one.
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New printer options:
With the upgrade 2.0.0-009 or higher, we provide you with two USB drivers:
- 1st driver is a PCL3 driver, common to many USB printers.
- 2nd driver is a PCL6 driver, used by Laser Color printer like HP Color
Laserjet 2605.
How to install this driver:
 Plug your USB key on a computer.

 Create the folder UPDATE in the root folder M18, and the sub-folder
PRTDRV, respecting the size of the letters.
 Upload the driver in the PRTDRV folder.
 Plug your USB key on the Mythic 18.
 Go to Set-Up.
 Select PRINTER 3 UPDATE.
 From the page

displayed, select the
driver you need to
install and press
LOAD.
 Validate the message
of confirmation.
 After uploading, go to
PRINTER and select
the printer you
installed.
 To finish, select the
format of the paper.
o 12” = A4
format
o 11” = US
Format
o 6” = A4 format, 2 results per page
o 5,5” = US Format, 2 results per page.
In the Advanced Set-up you can select 3 new options in the Printer menu:
 Automatic Print-out Analysis:

o Check box activated: ON
o Check box inactivated: ON DEMAND
 Automatic Print-out Start Up:
o Check box activated: the result of the background is
automatically printed.
o Check box inactivated: No print.
 Automatic Print-out QC:
o Check box activated: QC run is automatically printed.
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o Check box inactivated: QC run not printed.
Archive of patients:
In the previous version, all the patients were archived in the same table from
1 to 500 (max.).
With this new version, each patient result is archived in list of the day with
the following architecture:
- YY/MM1
o dd1
o dd2
o etc …
- YY/MM2
o dd1
o dd2
o etc …
- etc …
Step 1:
To display one result for one patient, you just
need to load the list of the day while pressing the
DATE button in the ARCHIVE menu.
Step 2:
Select either “INTERNAL MEMORY” or
“MASS STORAGE”
INTERNAL MEMORY = Mythic 18 memory
MASS STORAGE = USB Drive.
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Step 3:
The screen is shared in two parts:

- On the left, the list of the year and
months in the memory,
- On the right part, the list of days for
the selected month.
By default, the current month is

displayed.
Select the day while pressing on it then,
press in VIEW. The list of results for this
day is displayed.
From the archive menu, it is possible to save some results from the internal
memory to the USB drive.
To proceed, press on TOOLS from the main screen of the archive menu, select
the days you want to save, press SAVE.
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New communication protocol
With the update version 2.0.0-009 or higher, it is now possible to connect the
Mythic 22 either with RS232 cable or using TCP/IP protocol.
This new option is available in Set-up, Advanced, and Communications.
- Host serial = RS232 connection

- Host net = TCP/IP protocol
- SVM serial = to connect to SVM
through RS232 connection.
- Once you select one option, you can

define the setting for each option.
o SERIAL PARAM. For RS232
connection
o NET. PARAM. for Network
connection
- For the SERIAL PARAM., normally

you don’t need to modify the default
setting.
- For the NET. Parameters, select

UDP/IP or TCP/IP protocol, then input
the different requested information
about the host and the instrument
(Automaton).
- The MAC address is defined on the

CPU board. If no MAC address is
displayed, please contact us.
Due to the new functionalities on the Mythic 22, please refer to the document
“CP.pdf” to modify the communication protocol for the existing connection.
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Operator Screens – Main Menu
Toolbar available in each

menu:
Menu: Go back to Main

Menu
Previous screen: Come
back to the previous
menu.
Tools: Print, Send, Save,
Delete.
Date/time: By pressing,
gives the following
informations.
Mythic S/N
Password
SW version
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Operator Screens – Reagents
Note: Either you change one reagent and you prime this
reagent or you change all the reagents and you prime all.
Input
The lot diluent
Expiry date
Volume of
container in ml.
Press Change
Reagent to
confirm.
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Operator Screens – Calibration
1. 2. Input lot, targets, limits or

press Load to load these data
from USB key
3. Press load on the previous menu 4. Press Result and run calibrator
and select a lot. at least in triplicate.
Press Calibration to calibrate.
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Operator Screens – Quality controls
1. Choose one lot 2. Input lot, targets and limits, or press

load to load a lot from USB key
3. Press Load on the previous menu, 4. Run your control selecting Result
and then, select the file you need
to load, finish by Load.
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Operator Screens – Set Up
Only available with biologist

password
Communication default parameters :
Format : Shell
Bauds : 115200
Parity : None
Stop Bit : 1
Flow : No
Length : 8
Refer to Service Manual for the output

format to connect Mythic™22 to LIS.
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Operator Screens – Set Up
Default parameters :
Shut down : 180 mn.

Needle Up : 5 mn.
Valid Autoclean : Activated
Clean frequency : 80
Valid. Wake Up unchecked
Pressure checking Check
Note: if the customer performs more than

100 tests per day, let the autoclean
activated.
The shut down inactivates the test counting
for autoclean.
Activate the reagent checking to check the

validation of the expiry date as well as the
remaining volume of the reagents.
To avoid waiting 30 minutes during the warm

up on the morning, we recommend checking
the option Valid. Wake Up and input the
time.
The pressure checking option can’t be

modified by the customer, only by the service
engineer
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Operator screens – SVM Connection
Set Up of the SVM in the

Mythic™22 menu:
Set Up.
Advanced.
Communication.
Check the radio button SVM
Serial.
Connect the RS232 cable between Mythic™22 and Computer.
The cable configuration should be as described below.
Mythic 18 PC
DB9 male DB9 Female
1 --------------------------------------------------------------------- 1 CD
2 --------------------------------------------------------------------- 2 RXD
3 --------------------------------------------------------------------- 3 TXD
4 --------------------------------------------------------------------- 4 DTR
5 --------------------------------------------------------------------- 5 GND
6 --------------------------------------------------------------------- 6 DSR
7 --------------------------------------------------------------------- 7 RTS
8 --------------------------------------------------------------------- 8 CTS
9 --------------------------------------------------------------------- 9 RI
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Technician Menu
This entire menu is available for all the users.
Control Cycle: To use after an emergency

stop.
Motor Init: To reinitialize the 3 steppers
motors.
Hydraulics: To clean the system in case of
clogging.
Mechanics: To check the entire mechanical
part of the instrument.
Repeatability: To test the repeatability of the
instrument. (up to 20 replicates)
Log Errors: All the errors from the
instruments.
Technician: Only available with technician
password.(See next section)
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Technician Menu – Mechanics
Pressure:
In the stand by mode, the pressure is 0.
To check a leak of pressure:
Run a cycle (Run Analysis)
Stop the cycle by pressing the ON/OFF button,
when pistons are in medium position.
Go to Check Sensors.
Check the value for Pressure.
This value must be unchanged along the time.
If the pressure decreases, check the tubing and
the syringe block.
HGB sensor:
If the LED is OFF, the value must be around 0.
If the LED is ON (turn ON in the previous
menu), the value must be between 850 and
950.
WBC and RBC sensors:
In Stand by mode, the values are less than 30.
These values are measured directly on the
preamplifier board.
If one or both values are higher (higher than
100), check for interferences inside the machine
or check for an interference source outside the
machine.
Temperature:
3 sensors allow checking the temperature inside
and outside the instrument.
Temperature (Near Pressure): gives the room
temperature.
Enclosure Temperature: gives the temperature
inside the machine.
Reagent temperature: gives the temperature in
the reagent heater
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Technician Menu – Technician part
Note: This part is only available with the technician password: 2TEC
Only available with super technician

password.
CO=Aperture Current.
To check the current inside the counting
bath.
Turn 0N by pressing the button.
Open the fluidic door.
Put a pin of the voltmeter inside the courting
bath, a pin to the ground.
Measure the voltage.
For WBC chamber, the value should be

around 9V.
For RBC/PLT chamber, the value should
be around 13V.
If the values are different, either change the
counting chamber or the CPU board.
NOTE: The different sub-menus will be developed in the different

sections, hereafter.
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Technician Menu – Technician part
To adjust the electronic gains for WBC, RBC

and PLT.
To adjust the HGB blank.
See section Electronic part.
To clean the entire instrument before

packing.
Note: Never travel with liquid inside the

instrument.
To unlock the instrument
To adjust all the parameters of the optical

bench
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Section 3: Mythic™22 fluidic
 Fluidic diagram.
 The different modules.
o Sampling module
o Counting module
o Syringe module
 Troubleshooting on the fluidic part.
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Learning Objectives
Section 3 will explain the Mythic™22 fluidic. After reviewing this

section of the workbook you should be able to do the following:
 List the main modules of the instrument.

 Describe how to replace a sample probe.
 Describe how to change the O-rings.
 Describe how to fix a clogging problem.
 Describe how to diagnosis and fix a pressure leak.
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General fluidic diagram
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Fluidic cycles description:
Sampling cycle
1- Syringes down: (no valve)

~16.7 µl of blood sample are draw inside the sampling
needle
Diluent prime
Vacuum prime
2- Needle up and syringe up (valve 3+4 ON)

Needle outside cleaning with diluent (~0.7 ml) and waste
vacuum
3- Rocker move above the WBC bath and the syringes up to

drain the waste (valve 7 ON)
4- Needle down to the rinsing position
5- Syringe down
WBC bath drain (valve 1 ON)
Tubing transfer drain (valve 11+14 ON)
6- Syringe up
Second outside needle cleaning (~1.5 ml) (valve 4 ON)
Waste syringe drain (valve 7 ON)
7- Syringe down
8- Syringe up
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Sample dilution
WBC Dilution
9- Needle down to the dilution position and the syringes down

to prime the diluent (valve 8 ON)
10- Syringe up for the WBC dilution (~2 ml)

Diluent outside of the needle (valve 4 ON)
Diluent inside the needle (valve 4+5 ON)
Bubble to mix the dilution (valve 1 ON)
RBC dilution and lysing
11- Syringe down (no valve)

Drawing sample of the first dilution (~25µl)
Diluent prime
Vacuum prime
12- Needle up and syringe up

Rocker move above the RBC bath
Needle outside cleaning with diluent (~0.5 ml) and waste
vacuum (valve 3+4 ON)
13- Needle down to the rinsing position
14- Syringe up
Second outside needle cleaning (~0.9 ml) (valve 4+8 ON)
Lysis in the WBC bath (0,73 ml) (valve 9 ON)
Bubble to mix the dilution (valve 1 ON)
15- Syringe down

RBC bath drain (valve 2 ON)
16- Needle down to the dilution position
17- Syringe up for the RBC dilution (~2 ml)

Diluent outside of the needle (valve 4 ON)
Diluent inside the needle (valve 5 ON)
Bubble to mix the dilution and diluent to rinse the counting
head (valve 6+10+2 ON)
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Measurement:
18- Syringe down to process the vacuum counting (valve 8

+10 ON)
19- First measurement sequences, CBC 2 seconds (valve 10

ON)
20- Syringe up
Diluent back pressure (valve 4+6 ON)
21- Syringe down to perform the vacuum counting (valve 8

+10 ON)
22- Second measurement sequences (valve 10 ON)

CBC Measurements, 8 seconds (valve 10 ON )
Syringe up for DIF measurements, 12 seconds
(valve 4+9+12+13+16+17)
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Cleaning and drain
Drain and Rinse bath
23- Cleaning
Cleaning the apertures (valve 11 ON)
Back pressure in the injector (valve 4+13+14 ON)
24- Syringe down

Tubing transfer drain (valve 11+14 ON)
RBC bath drain (valve 2 ON)
25- Syringe up
RBC bath rinse diluent (~2 ml) (valve 4 ON)
26- Needle up and syringes up for back flush (valve 4+6

ON)
27- Rocker move above the WBC bath
28- Syringe up
WBC bath rinse diluent (~2.5 ml) (valve 4 ON)
Needle back and waste drain
29- Syringe down

Transfer tubing rinsing with ~0.5 ml (valve 11+14 ON)
Dry the outside of the needle (valve 3 ON)
30- Rocker move in the sampling position
31- Syringe up
32- Needle down in sampling position
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Fluidic modules: Sampling module
Rep Part Number Designation

1 005-1001-02 01 Sampling module
2 005-1001-02 03 Rocker Motor
3 005-1001-02 02 Needle Motor
4 005-1001-02 04 Needle rinsing head
5 010-2501-02 05 Sampling Needle
6 312-0505-140 125 O-ring Ø1.4x1.25 Fluocarbon 80SH
7 010-2501-02 02 Needle carriage
8 353-0301-08 120 19 Belt pitch 2.032 (l=4.763-L=243.84)
9 005-1001-82 880 300 Needle Optical Sensor
10 363-0201-030 080 Screw K 30 x 8
11 360-0102-030 060 Screw RLX M3 x 6
12 360-0101-030 080 Screw CZX M3 x 8
13 005-1001-82 880 200 Rocker Optical Sensor
14 370-4050-030 10 Anti-loss washer Ø3 x 1
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Fluidic modules: Counting module

1 005-1001-01 01 Counting chambers
2 010-2501-01 03 Counting manifold
3 005-1001-01 02 01 WBC Counting Head
4 005-1001-01 02 02 RBC Counting Head
5 312-0505-500 100 O-ring Ø5x1 Silicone 80SH
6 360-0101-030 060 Screw CZX M3 x 6
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Fluidic modules: Syringe module

1 002-1001-03 05 Syringes block
2 010-2501-03 06 Seal plate - Syringes module
3 010-2501-03 02 Piston Ø 16 - Syringes module
4 005-1001-03 03 Piston Ø 22
5 010-2501-03 01 Piston Ø 6.5 - Syringes module
6 005-1001-03 09 Syringe Piston Ø 1.6
7 312-0501-1554 262 O-ring Ø15.54x2.62 Silicone 60SH
11 010-2501-03 10 O-ring spacer
12 363-0201-030 100 Screw K 30 x 10
Note1:
For
03/09/2009 - 53 -
replacement of the O-rings on the syringe block, please refer to the
Mythic™18 service manual.
Note2: The O-rings on the seal plate of the syringes module are
greased in the factory, as well as the pistons. It is very important
that the greasing of these parts is maintained all the time. We
recommend greasing the pistons every 6 months and the O-
rings once per year. The pistons can be greased by the customer.
A default of greasing could be suspected in the following cases of

troubles:
- Trace of liquid under the syringes block.

- Leak of pressure.
- Abnormal noise during the movement of the pistons.
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Fluidic modules: Tubing connection
View n° 1 - Counting manifold
View n° 2 - Syringes manifold
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View n° 3 - Syringes body
03/09/2009 - 56 -
Fluidic troubleshooting: Clogging
When could you suspect this problem?
A clogging could be suspected in the following cases:
- Rejected result on WBC, RBC, PLT.

- Drop of blood on the needle.
- Rejected result on WBC, RBC, PLT:
The result of one or more parameters appears as below:
WBC: --- *
RBC: --- *
PLT: --- *
Or
WBC: Value with “*”

RBC: Value with “*”
PLT: Value with “*”
In any case, the flags “W_CL” or “R_CL” appear in the flag box
below the results.
W_CL means WBC Clog.

R_CL means RBC/PLT clog.
To fix the problem:
1/ Rerun the sample.
2/ If the problem continues, follow the next procedure:
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Service Menu
Hydraulic
Cleaning
End of
OK Run Sample
service
NO
Backflush
OK
Run Sample
NO
Bleach with hypochloride

solution 10%
OK Run Sample
NO
1/ Dismantle the counting

head.
2/ Remove the o-ring.
3/ Place the counting head
OK in bleach solution for 10
minutes.
4/ Rinse the counting head
with distilled water
5/ Replace the counting
head
Replace the counting head

by a new one
Electronic gain adjustment
03/09/2009 - 58 -
- Drop of blood on the needle.
It could happen that you observe a drop of blood falling from the
needle after the blood aspiration.
Note: As we explain before, none of the valves is used during this

part of the cycle. So, it is not a problem of valve.
To fix the problem:
This problem appears when a clog is located in the rinsing head of

the probe.
Rinsing
head
Tubing
n°10 :
Tubing n°21 :
Aspiration
Diluent tubing
tubing
Follow the next procedure:
03/09/2009 - 59 -
Remove the rinsing head from the rocker
1/ Dismantle the needle,

2/ unscrew the roller which maintains the O-ring,
3/ remove the O-ring.
4/ Disconnect the 2 tubing: n°10 and 21
1/ Place the rinsing head in hypochloride solution

for 5-10 minutes.
2/ Rinse it with distilled water
Replace the rinsing head and the needle.

Don't forget the o-ring
End of service OK Run Sample
NO
Replace the tubing n°10, aspiration tubing.
03/09/2009 - 60 -
Fluidic troubleshooting: Leak of pressure
- HCT result rejected or too low (not proportional with Hb result).

Impossible to recalibrate.
- Repeatability problem on the results.
- Message “Default Pressure”, follow with emergency stop.
How to fix problem with HCT results too low or repeatability

problem?
1- Diagnosis
Follow the next procedure:
03/09/2009 - 61 -
03/09/2009 - 62 -
Login as Technician
Open the door of the instrument
Run analysis
Emergency stop when pistons on down position
1/ Service Menu
2/ Mechanics
3/ Check sensors
PRESSURE Check the value of the pressure. Should be stable along the time.
Check leak of liquid
STABLE If the pressure is decreasing, leak of pressure
PRESSURE UNSTABLE
Dismantle the seal plate of the syringes module.

1/ If the o-rings are old, replace them by new ones.
2/ If not, grease the o-rings.
Repeatability OK Run Sample
NO
HCT calibration OK Change valve n°4
End of service
03/09/2009 - 63 -
Fluidic troubleshooting: Leak of pressure
How to fix problem with “Default pressure” message?
The message “Default pressure” is generated if the option “pressure

checking”
is activated in the menu “Set Up”, “Others settings”.
This message is displayed if the system detects a default of vacuum

during
the counting cycle or during the drain cycle of the chambers.
Different cases could activate this alarm:
- Diluent container empty (Option “Checking Reagents” not

activated).
- Tubing disconnected.
- Leak on the waste syringe.
Before to perform a control cycle,
 Check the volume of diluent in the container.
 Open the door of the instrument and make a visual check of

the fluidic
part to detect any tubing disconnected or leak of liquid.
03/09/2009 - 64 -
03/09/2009 - 65 -
Section 4: Mythic™22 Electronic
 Electronic diagram.
 Boards description.
 Wiring description.
 Valves replacement.
 Electronic gain adjustment.
03/09/2009 - 66 -
Learning Objectives
Section 4 will explain the Mythic™22 Electronic part. After reviewing

this
 List the 3 boards of the instrument.

 Describe how to replace a main board.
 Describe how to calibrate the touchscreen.
 Describe how to adjust the electronic gain.
03/09/2009 - 67 -
Mythic™22 Electronic: General electronic diagram
03/09/2009 - 68 -
Mythic™22 Electronic: Main Board – Main components
03/09/2009 - 69 -
CMD Rocker
+60 V DC
CMD Nee
+3.3V DC
CMD Syring
CMD Count
EV
+ 5V DC
Pulse proce
logic
DRAM
Printer driver microprocessor Flash memory
03/09/2009 - 70 -
Mythic™22 Electronic: Main Board – Voltage check points
+3.3 V DC
+ 5 V DC
+60 V DC
03/09/2009 - 71 -
Mythic™22 Electronic: Preamplifier board
03/09/2009 - 72 -
Mythic™22 Electronic: GUI board & Calibration of the
touchscreen
NOTE:
It could be useful to recalibrate the touchscreen. To do this

calibration:
 Press in the same time on the numerical keyboard the key

“.” and “DEL”.
 A cross appears on the display.
 Press in the middle of the cross.
 Proceed as previously with the next cross which appears.
 When the calibration is done, the display comes back to its
initial status.
03/09/2009 - 73 -
Mythic™22 Electronic: General wiring
03/09/2009 - 74 -
Mythic™22 Electronic: Main wiring
Door switch
Syringe EV Needle Optical

Sensor
Counting EV
Rocker Optical
Sensor
Needle motor
Motor
syringe Rocker motor
Syringe Optical
Sensor
NOTE: For the wiring of the different parts (sensors, motors,

valves …), please refer to the Mythic™22 service manual.
03/09/2009 - 75 -
Mythic™22 Electronic: Valves replacement
Note:
In order to proceed to the valves replacement we recommend

using the following material:
 Dynamometric screwdriver 15-75 cNm (Facom A.301MTJ1
or equivalent)
 Torx plug 6
1. Counting chamber valves replacement
03/09/2009 - 76 -
Mythic™18 Electronic: Valves replacement
2. Syringes module valves replacement
WARNING
EV 3/2 Ø 1.6mm
03/09/2009 - 77 -
Mythic™22 Electronic: Electronic gain adjustment
Note1:
The electronic gain adjustment should be done after replacement

of the following parts:
 Main board
 Preamplifier board
 Counting chamber
 Counting heads
Note2:
The electronic gain adjustment doesn’t replace the calibration of

the parameters with a blood calibrator.
03/09/2009 - 78 -
1. WBC Latex (Diameter : 7µ)
Spare Parts Changed Action
PCB Board WBC Electronic Gain Adjustment
Preamplifier Board WBC Electronic Gain Adjustment
Counting Chamber WBC Electronic Gain Adjustment
WBC counting head WBC Electronic Gain Adjustment
Flow cell Optical gain adjustment
2. RBC/PLT Latex (Mix 1/1 Diameter 3µ/5µ)
Spare Parts Changed Action
PCB Board RBC/PLT Electronic Gain

Adjustment
Preamplifier Board RBC/PLT Electronic Gain

Adjustment
Counting Chamber RBC/PLT Electronic Gain

Adjustment
RBC/PLT counting head RBC/PLT Electronic Gain

Adjustment
03/09/2009 - 79 -
You need to login in TECHNICIAN MODE (enter the TECHNICIAN password).
- Select the SERVICE option

- Select the TECHNICIAN option
- And select PROD ADJUST
Report the diameter for WBC
Report the diameter for RBC

and PLT
- Shake the WBC LATEX with a VORTEX system

- Place the tube under the sample needle and press LATEX WBC
- Wait the end of the cycle and check that values are inside the following range:
03/09/2009 - 80 -
Mythic 18 Gain Mean Range
WBC Between 210 and 242 Between 66 and 72
WBC Between 160 and 220 Between 53 and 72
- Shake the RBC/PLT latex with a VORTEX system

- Place the tube under the sample needle and press LATEX RBC PLT
- Wait the end of the cycle and check that values are inside the following range:

RBC Between 210 and 250 Between 29 and 42
PLT Between 215 and 250 Between 48 and 52
RBC Between 160 and 220 Between 38 and 50
PLT Between 160 and 220 Between 48 and 65
- When it is OK press VALID and confirm SAVING IN MEMORY
03/09/2009 - 81 -
Electronic troubleshooting: WBC, RBC or PLT bad results?
03/09/2009 - 82 -
03/09/2009 - 83 -
Section 5: Mythic™22 mechanic
 Syringe motor sub-assembly and greasing
 Sampling module and greasing
03/09/2009 - 84 -
Learning Objectives
Section 5 will explain the Mythic™22 mechanic part. After reviewing

this
 List the main mechanical parts of the instrument.

 Describe how to grease the main part of the syringe module.
 Describe how to grease the sampling module
03/09/2009 - 85 -
Mythic™22 mechanic: Syringe motor sub-assembly
2 parts needs to be greased during the preventive maintenance:
- The endless screw with the grease “Lubriplate DS-ES” ref.

410-0502-01.
- The guide of the pistons holder with the grease “Plastic grease
SPG35” ref. 410-0503-01
03/09/2009 - 86 -
Mythic™22 mechanic: Sampling module
2 parts could be greased during the preventive maintenance:
- Axis of the rocker  with the grease “Plastic grease SPG35”

ref. 410-0503-01
- The rail of the rocker  with the grease “Plastic grease
SPG35” ref. 410-0503-01


03/09/2009 - 87 -
Section 6: Mythic™22 optical part
 Optical manifold.
 Optical bench.
 Transverse adjustment.
 Optical electronic gain adjustment.
03/09/2009 - 88 -
Learning Objectives
Section 6 will explain the Mythic™22 optical part. After reviewing

this
 Describe how to change an entire optical bench.

 Describe how to perform the transverse adjustment.
 Describe how to adjust FSC and ALL gains.
03/09/2009 - 89 -
Important Note:
Today only the complete optical module (Optical bench, P/N: 005-1002-
01 01) or the flow cell (Optical flow cell P/N: 005-1002-02 01) can be
replaced on customer site.
It means that it is impossible to replace independently the illumination or

detection sub-assembly which composes the optical bench and it is
obvious that it is impossible to replace independently the different parts
which compose these sub-assemblies.
Those sub assemblies are adjusted in production area on a specific

benchmark which allows to control each part independently and to adjust
the complete optical bench.
The only adjustment required after module or flow cell replacement is the
transverse lens support adjustment, as we well see hereafter
03/09/2009 - 90 -
Mythic™22 Optical part: Optical Manifold
1. Tubing position
2. Valves position
03/09/2009 - 91 -
Mythic™22 Optical part: Optical Manifold
Tubing 48: Note that it is

very important in case of
change of this tubing to
respect the loop made by this
tubing.
The loop create a micro-
bubbles catcher and avoid
these bubbles go to the
injector.
03/09/2009 - 92 -
Mythic™22 Optical part: Optical Manifold Troubleshooting
No scattergram of too few points displayed on screen after

blood analysis
1. Check all solenoid valves (Service > Mechanics > Check Valves>Open/close). You
must hear distinctly the state changing.
2. Check the different steps in the WBC bath (empting, filling, dilution,
homogenization, lysing, etc…)
3. Check if the tubing #48 makes the loop against the illumination part.
This position avoid pushing micro bubbles in the injector during the measurement,
those bubbles will stay in the high part of the loop and will be evacuated at the
end of the cycle.
4. Check if the tubing #48 is emptied and filled properly during the cycle.
a. At the beginning of the cycle, at the end of the WBC bath emptying, the
tubing #48 must be completely empty.
b. During the cycle, just after the second homogenization in the WBC bath,
the tubing #48 must be completely filled with the blood sample present in
the WBC bath.
If it is not the case:
 Check if the hydraulic “T” on the WBC bath is not damaged, which
could cause a pressure difference.
 Check tubing # 56, 55 and 47 (refresh if necessary)
 Check tubing # 37, 5, 6 (they could be pitched when the fluidic
door is closed, which could generate problems for the tubing #48
empting and filling)
03/09/2009 - 93 -
Mythic™22 Optical part: Optical bench
Optical Bench Functioning Generalities
1 Origin of the alignment
Particles to be analyzed are transported by the 80µm cross section sample

flow (see Fig. 2.3c), they have to pass the optical interrogation zone where
light-particle interaction occurs.
In order to get the best possible SNR (Signal Noise Ratio) for the optical
measurement, dimensions of the optical interrogation zone have to be close to
the sample flow diameter .
Since optical interrogation zone is a rectangular area of 110µm  30µm and
sample flow has a diameter of 80µm, their relative positioning has a tolerance
of 15µm.
Such a tolerance can not directly be reached when stacking the several
mechanical parts of the optical bench: an alignment is thus required.
So, the alignment basically comes from the need of high accuracy positioning
of the light beam with respect to the sample flow.
2 Principle of the alignment
In the Mythic22 optical setup, the flow cell is static (i.e. the sample flow
position is fixed) but the light beam is shifted either longitudinally or
transversally (see Fig. 2.2a) for the optical interrogation zone to match the
sample flow.
2.1 Longitudinal alignment
This is a factory done process which requires specific equipments. The

longitudinal alignment is done once-for-all when the system is assembled and
never requires further adjustment.
In particular, maintenance or replacement of the flow cell has not impact onto
longitudinal alignment.
>> Never release illumination module screws <<
If screws that lock illumination module are released by mistake, the whole
optical bench has to be sent to the factory for full re-alignment.
2.2 Transverse alignment
The transverse position of the light beam with respect to the fixed sample flow
(see Fig. 1) has to be adjusted when the flow cell is removed and replaced. It
might also be necessary to adjust this position when unpacking the instrument
after shipping.
03/09/2009 - 94 -
Figure 1 - Light beam transverse shift
The light beam transverse position is adjusted by rotating the transverse

adjustment window. Any rotation (represented in green on Fig. 2) of the
window holder transversally shifts (represented in green on Fig. 1) the light
beam.
Figure 2 - Light beam transverse adjustment. Any

rotation of this feature translates the light beam
transversally
The sample flow nominal position is represented on figure 2.3c, the sample
flow transverse position is correct when its cross section is fully included inside
the interrogation zone as represented on figure 4.
Thus, the transverse adjustment is a process that allows to moves the light
beam from a random transverse position to the nominal one represented on
Fig. 4.
Transverse adjustment relies on the use of Latex beads with a mean diameter
of 7µm. Those latex beads have diameter variations of less than 1% from one
bead to the other, so they can all be considered as perfectly identical.
03/09/2009 - 95 -
Figure 3 - When particles pass the blue Figure 4 - When the sample
interrogation zone near the left or right flow is fully inside the zone,
boundaries, their amount of received particles can no more reach
light drops. the interrogation zone
boundaries. They are equally
illuminated
Since all latex particles are identical, it is obvious that light pulses generated
by the optics should also be identical when the system is properly aligned (as
represented on Fig. 6).
If the sample flow is not fully included in the interrogation zone (Fig. 2.2c),
some of the latex beads will not be fully illuminated as they are located at the
zone boundary (see particle #3 of Fig. 3) .
Figure 5 - The less the particle is Figure 6 - When particles are equally
illuminated, the weakest is the illuminated, all light pulses have nearly
amplitude the same amplitude.
So, when the system is not properly aligned, particles receive different amount
of light which, in turns, leads to pulses of various amplitude. Thus in that case,
difference between the strongest and the weakest pulse amplitude is quite
large as seen on Fig. 5.
When properly aligned, sample flow is fully included inside the interrogation
zone (Fig. 4) and all particles receive the same amount of light. Pulses
amplitude are then very similar and there’s only a very slight difference
between the strongest and the weakest ones (Fig. 6). This slight remaining
difference comes from the baseline residual noise (Fig. 2.3b).
03/09/2009 - 96 -
03/09/2009 - 97 -
2.3 Embedded alignment tool
The Mythic22 embedded tool for transverse alignment works on batches of 10

latex pulses. For every batch of 10 pulses, the MAX and MIN values are found
and plotted on the graph (see Fig. 2.3a and 2.3b). The MAX value is plotted in
red whereas the MIN value is plotted in black.
Figure 7 - When the system is not Figure 8 - When the system is

aligned, the black curve has a huge properly aligned, their are few
variation fluctuations of the black curve
(MIN).
The red curve is thus representing the MAX values of all packets of 10
particles whilst the black curve represents the MIN values of those batches.
When the light beam is not aligned into the interrogation zone, pulses
amplitude have a very high dispersion (Fig. 5 and 7) so the black MIN curve
values strongly varies over time since it reflects how the particle is outside the
zone.
When the system is properly aligned (Fig. 6 and 8) all particles are equally
illuminated and dispersion of the black MIN curve is very low.
03/09/2009 - 98 -
Figure 9 – Light beam nominal position with
respect to sample flow.
So to align transversally the light beam onto the sample flow, just rotate the
holder (Fig. 2) while both curves are updated. The transverse alignment is
done when fluctuations of the black curve (MIN) drops to the ones depicted
Fig. 8.
Remarks:
- On Fig. 7 and 8, the red curve shows peaks (encircled in green). Those peaks
occur when more than one latex bead enter the interrogation zone. Those
peaks (on the red curve only) have no effect onto the alignment accuracy,
they must be ignored.
- For information purposes, all values are read along one single channel: the
ALL one.
03/09/2009 - 99 -
Mythic™22 Optical part: Optical bench troubleshooting
No scattergram of too few points displayed on screen after

blood analysis
I. Problem from Flow cell
1. Check if an air bubble is not blocked at the top of the nozzle.
 Remove the flow cell protection.

 Run an empty cycle (without blood sample) and observe the top of the
nozzle during the measurement (blue light on)
 If a bubble is blocked at the top of the nozzle, those one diffract the light
and you can observe a very lighting blue spot at the nozzle exit.
 In this case, run back an empty cycle and pinch simultaneously the two
diluents tubing (# 52 and 53) just under the flow cell support during the
measurement. You will create a pressure difference which will unblock the
air bubble.
Repeat empty cycles few times in order to check if the problem comes back. If
it comes back, investigate on the hydraulic circuit to find the origin of the
bubbles.
2. Check if the nozzle is not blocked
Remove the flow cell from the module (2 screws RLX M3x6)
WARNING: BE CAREFUL, DON’T PUT FINGERS ON OPTICAL GLASS

PARTS
Connect the syringe (full of distilled water) at the entrance of tubing # 48 on

the cuve support.
Block the entrance of tubing # 47 on the cuve support.
Push on the syringe piston; you must see a straight jet of water which exit
from the nozzle.
a. If the jet is bent, means that the nozzle is partially blocked.

b. If there is no jet, means that the nozzle is totally blocked.
3. Check if the flow cell is not dirty, scratched or damaged.
 When the flow cell is damaged, the transverse support adjustment is very
difficult.
 The curves in the adjustment menu (OPTICAL ADJUST) are much noised,
so the automatic gains adjustment doesn’t work.
 It is possible sometime to see visual default on the optical parts after
removal.
 Try to clean external area of the 2 optical lens with an alcohol solution.
03/09/2009 - 100 -
II. Problem from optical module.
 Check if the diode runs properly.
Service > Mechanics > OPT. LED OFF/ON.
If no, check if the CPU board sends the power supply on the led connector
before to change optical bench (if no, change the CPU board)
 The CPU board is damaged.
 There is no pulse on the matrix result and you had checked all
previous items. Replace the CPU board.
 The FSC board is damaged.
 The transverse support adjustment is not possible. There is no

pulse and no curve in the adjustment menu (OPTICAL ADJUST).
Be careful if the FSC gain is too high the curve will be out of
screen (input a default gain equal to 130).
 The ALL board is damaged.
 The transverse support adjustment is not possible. There is no

pulse and no curve in the adjustment menu (OPTICAL ADJUST).
03/09/2009 - 101 -
TRANSVERSE SUPPORT ADJUSTMENT
 After complete module or just the flow cell replacement, run three empty
cycles in order to remove all air bubbles from the optical hydraulic circuit.
 Run a blood sample analysis and wait the result.
 You must see the matrix on screen.
 From the main menu, select “SERVICE”, “TECHNICIAN” and “OPTICAL

ADJUST”.
 Select « ADJUST LED » and wait the result of the adjustment.
WARNING: Be careful, this adjustment must be done with fluidic door

closed.
 The transverse adjustment must be done after the instrument reaches the
correct temperature.
 Place the 7.0 µm latex solution in sample aspiration position.
 In “OPTICAL ADJUST” select run and wait the result of the test.
03/09/2009 - 102 -
Note: The black and red curves must be located at the top position of the
graph; they must be as smooth as possible and must be as close as possible.
If it is the case, the transverse support doesn’t need any adjustment.
03/09/2009 - 103 -
 Place the 7.0 µm latex solution in sample aspiration position.
 In “OPTICAL ADJUST” select «LATEX WBC» and wait the result of the test.
 If it is not the case, gently release the transverse holder screw by executing 1
turn in CCW direction (Torx T06)
 Put the specific tool «Transverse Adjustment Tool» at its nominal position.
 Put the 7.0 µm latex solution in sample aspiration position.
 In “OPTICAL ADJUST” select run.
 When the curves appear, carefully turn the transverse holder in one way or
the other, in order to define the best adjustment position which gives the best
result regarding the black and red curves.
 Repeat this operation as long as necessary to obtain the good result.

 When the adjustment is made, screw back the transverse holder fixation screw
(1 turn in CW direction).
 Put back the Flow cell protection.
 Put the 7.0 µm latex solution in sample aspiration position.
 In “OPTICAL ADJUST” select «LATEX WBC» and wait the result of the test.
03/09/2009 - 104 -
 Press on «VALID» in order to save in memory the gain adjustment result.
 Back to the main menu and perform a run sample on the latex solution.
 At the end of the test, press on the screen to obtain a zoom of the matrix.
 You must get an oval mark in the left upper part of the screen as showed
below.
 Run sample with a fresh blood sample and wait for the result.
WARNING: BE CAREFUL, THIS TEST MUST TO BE PREFORMED AT THE

GOOD REGULATION TEMPERATURE, AFTER RECEPTION OF THE
MESSAGE “TEMPERATURE REACHED”
 At the end of the test, press on the screen to obtain a zoom of the matrix.
 It is possible to obtain a matrix with the different populations out of the

defined thresholds.
 To adjust properly the position of the matrix, you have to adjust manually the
FSC and/or the ALL gains values.
 From the main menu, SERVICE, TECHNICIAN, COUNTING PARAMETERS.
Note: The matrix moves to the right when the FSC gain value
increases, it moves to the left when the FSC gain value decreases.
03/09/2009 - 105 -
The matrix moves to the top when the ALL gain value increases, it
moves to the bottom when the ALL gain value decreases.
Gains values
 Modify the FSC and/or the ALL gains values in order to put clusters in these
respective boxes. (start with step of 10)
 Press on “VALID” to confirm and “Yes” to save in memory.
 Re-run the same blood sample to see the difference.
 Adjust FSC and/or ALL gains values until you get the right matrix positioning
in accordance with the thresholds.
03/09/2009 - 106 -
03/09/2009 - 107 -
Section 7: Mythic™22 heater
 Different parts of the Mythic™22 heater.
 How to dismantle the heater.
 Temperature flagging.
 How to unlock the Mythic™22.
03/09/2009 - 108 -
Learning Objectives
Section 7 will explain the Mythic™22 heating module. After

reviewing this
 Describe how to dismantle the heater.

 Describe the different cases of locking the system.
 Describe how to unlock the instrument.
03/09/2009 - 109 -
Mythic™22 heater: the different parts of the
module.
Enclosure heater
Heating FAN
Diluent output
Reagent heater
Diluent input
Lyse output
Lyse input
Lyse heating circuit
Diluent heating circuit
03/09/2009 - 110 -
Mythic™22 heater: Temperature regulation
and associated flagging
Note: Three sensors detect the temperature inside and outside

the Mythic™22. One is located in the enclosure, connected on the
preamplifier board; one is located in the heater; the last one is
located under the reagent tray to detect the room temperature.
SENSOR OBSERVED ACTION MESSAGE RUN

TEMPERATURE ANALYSIS/CAL/QC
REAGENT No increasing M22 Heat NO
during 5 min. Switch Reagent
Off Failure
REAGENT > 60°C Heater Hardware: NO
Stopped Heat
reagent
stopped
REAGENT > 70°C M22 Hardware: NO
Switch Heat
Off reagent
M22 Failure
locked
ENCLOSURE No increasing M22 Heat NO
during 5 min. Switch Enclosure
Off Failure
ENCLOSURE > 50°C Heater Hardware: NO
Stopped Heat
enclosure
stopped
ENCLOSURE > 55°C M22 Hardware: NO
Switch Heat
Off enclosure
M22 Failure
locked
AMBIANT < 17°C Sampling Temperature NO
needle out of range
not go
down
AMBIANT > 35°C Sampling Temperature NO
needle out of range
not go
down
REAGENT/ENCLOSURE < 34°C or > Sampling Temperature NO
45°C needle out of range
not go
down
03/09/2009 - 111 -
Mythic™22 heater: How to unlock the
system
Note: when the system is locked, only the service engineer can unlock it.
To unlock the instrument, enter the service password (2TEC), and then follow
the next procedure.
Service Menu
Technician Menu
Thermal
Press on "Unock Heat"
The system is unlocked,

Check the temperature sensors in "Check Sensors"
in the Mechanic Menu.
T° doesn't increase correctly Temperature increases correctly
Heater is Ok, preamplifier

Change the heater board or Main board defective
(Depending on the alarm)
03/09/2009 - 112 -
Section 8: Mythic™22 Technical Notes
03/09/2009 - 113 -
INSTALLATION RECOMMENDATIONS
Objective of this technical note:

To give the distributors a guide line to install in good conditions the Mythic 22 OT.
Notice: temperature in the Lab must be between 20° C and 30°C max
The power current with the Mythic must be grounded or (and) the back of the instrument is
grounded properly
Procedure:
1- Unpack the system
2- Plug the power supply
3- Plug reagents
4- Open the door
a. Remove the foam
b. Check needle in the correct position
c. Check counting chamber and counting head in correct position and close the door.
5- Switch on
6- Technician password
7- Set-Up:
a. Date/time in local format
b. Printer (if needed)
c. Print-Out options
d. Lab parameters (if needed)
e. Others options:
i. Autoclean (depend off the load of the instrument: <50 samples/day = 80
(default) , >50 samples/day = 25)
ii. Wake up (min. 30 minutes before sampling work)
iii. Shut down time (time after last measurement, depend of laboratory schedule
and workload )
f. Storage Options: FIFO mode activated if memory stick don’t use.
03/09/2009 - 114 -
8- Reagents :
a. Expiry date
b. Lot number
c. Volume
Waste: capacity (stand on the floor)
9- Diluent: always stand at same level of the machine.
10- Prime All x 2
11- Start-up (check ground values following the user manual)
12- Calibration ( 3 replicates at least)
13- QC
14- Repeatability (check CV, report to the user’s manual for acceptable range )
03/09/2009 - 115 -
Adjustments to perform using latex and G-Cal

Make a summary of the different adjustments to perform after a service or maintenance on Mythic
22 and 22 CT.
Procedure:
1- With WBC latex if change of:
a. CPU Board
b. Preamplifier board
c. WBC counting head
d. Counting chamber
e. Flow cell
f. Optical bench
2- With RBC latex if change of:
a. CPU board
b. Preamplifier board
c. RBC counting head
d. Counting chamber
3- With G-Cal if change of:
a. Flow cell
b. Optical bench
WARNING: When latex is performed to adjust the WBC, RBC and PLT gain, it is
important to calibrate these parameters using Myt-CAL from Orphée.
03/09/2009 - 116 -
MAINTENANCE PROGRAM

To define a maintenance program for Mythic 22 OT, in order to avoid problems on the system.
To define a guideline applicable by every service engineer.
Procedure:
03/09/2009 - 117 -
Workload < 50 samples/day
Every 4 months
Short maintenance program
Check points:
- O-ring needle (rinsing head)

- O-ring counting head
- All tubing connections
- Connectors preamplifier board – counting chamber
- Adjust HGB, check the value between 800 – 900
- Adjust LED (optical adjust), higher than 250
Greasing:
Mechanical grease:
- Endless screw
- Guide piston motor
- Guide needle
Plastic grease:
- Guide endless screw

- Rocker guide
- Rocker wheel
Silicon grease:
- Syringes
- O-ring counting chamber
03/09/2009 - 118 -
Cleaning:
- Counting chambers (be careful with the electrode inside the chamber)
- Screen
- Bleach
Adjustment:
- WBC and RBC/PLT latex check.

- If needed, calibration.
- Latex WBC optical (every 2 maintenances): See Mythic 22 workbook for the full
description of the procedure.
- G-Cal. See TB-09-01 for the procedure
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Workload > 50 samples/day
Or 24 hours to 24 hours
Every 3 months
Short maintenance program
Check points:
- O-ring needle (rinsing head)

- O-ring counting head
- All tubing connections
- Connectors preamplifier board – counting chamber
- Adjust HGB, check the value between 800 – 900
- Adjust LED (optical adjust), higher than 250
Greasing:
Mechanical grease:
- Endless screw
- Guide needle
Plastic grease:

- Rocker guide
- Rocker wheel
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Silicon grease:
- Syringes
- O-ring counting chamber
Cleaning:
- Screen
- Bleach
Adjustment:

- If needed, calibration.
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Workload < 50 samples/day
Every two years
Main maintenance program
Change:
- All of the tubings (tubing kit)

- All of the O-rings (O-ring kit)
Dismantling:
- All valves and check

Greasing:
Mechanical grease:
- Endless screw
- Guide needle
Plastic grease:

- Rocker guide
- Rocker wheel
Silicon grease:
- Syringes
- O-rings
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Cleaning:
- Screen
- Bleach
Adjustment:

- Calibration.
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Workload > 50 samples/day
Or 24 hours to 24 hours
Every year
Main maintenance program
Change:
- All of the tubings (tubing kit)

- All of the O-rings (O-ring kit)
Dismantling:
- All valves and check

Greasing:
Mechanical grease:
- Endless screw
- Guide needle
Plastic grease:

- Rocker guide
- Rocker wheel
Silicon grease:
- Syringes
- O-rings
Cleaning:
- Screen
- Bleach
Adjustment:

- Calibration.
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List of spare parts, reagents and tools required:
- Minimum Tools:
o Torque driver with plug of 6, 10 and 20.

o Flat screwdriver.
- Spare Parts:
o M18-M22-M22CT O-rings kit, code number: 002-1005-50 10

o Mythic 22 Tubing kit, code number: 002-1002-50 11
o Kit latex, code number: 465-0110-05
o Silicon Grease, code number: 410-0501-01
o Lubriplate DS-ES, code number: 410-0502-01
o Plastic Grease SPG35, code number : 410-0503-01
- Reagents:
o Myt-Cal, code number: Myt-Cal2

o Myt-5D Normal level or tri level, code number: Myt506 or Myt502
o G-Cal, code number: G-Cal
- Bleach solution (hypochlorite solution), max. 10% of chloride.

- Distilled water.
- Last updated version of software.
03/09/2009 - 125 -
Sensors and electronic gain values
Check sensors menu:

- Pressure:  0 in standby mode
- WBC < 15 (little fluctuations are normal)
- RBC < 25 (little fluctuations are normal)
- HGB:
o LED OFF and all the covers closed: 0
o LED ON and all the covers closed: 800 to 900
- FSC (LED ON):  200
- Reagent temperature: 34°C (must be in accordance with thermal setting)
- Enclosure temperature: 36°C (must be in accordance with thermal setting)
- Both set of temperatures: AUTO mode.
Gain Values, Results reading and channels in Prod Adjust and Optical Adjust
Parameters Gain Values Results reading Channels Comments

Optical LED 0 - 255 ≥250
HGB LED 150 - 255 800 – 900
RBC 160 – 220 43 Depends on diameter
PLT 160 – 220 55 Depends on diameter
WBC 160 – 220 59 Depends on diameter
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How to use and interpret the ratio O-CL (Only available from version 2.3.0)
With super-technician password, the ratio for the optic is added from Run Sample
Menu. See description below to check this value.
i. From patient result, press on the scatter gram to focus on it.
ii. From the focus, press on DIF.
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iii. Check the ratio in O-CL.
iv. How to interpret the value O-CL?
If the ratio is low (<30%), it means that the nozzle is clogged. Generally, the
flag O-CL will appear on the result of the patient.
The ratio O-CL enables to follow the status of the injector. With a new
machine, the ratio should be between 75% and 125%. During the time, if this
value decreases, it means that the nozzle could be partially clogged or
clogged:
- If 10%<O-CL<30%: Partially clogged (flag OPT-)
- If O-CL<10%: Clogged (flag O-CL)
- If the value is 50% on different samples (don’t take the value on one
sample), clean the system while bleaching.
03/09/2009 - 128 -
Mythic™22 Service engineer training workbook
Section 9: Mythic™22 Cap Piercing module
 Different parts of the module.
 Difference between Mythic 22 OT and Mythic 22 CT.
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Learning Objectives
Section 7 will explain the Mythic™22 Cap piercing module. After reviewing this
 Explain the main differences between Mythic™22 OT and Mythic™22 CT.

 Describe the piercing cycle.
 Describe how to dismantle the module.
 Describe how to adjust the module.
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Mythic™22 CT: Main differences between

Mythic™22 OT and Mythic 22 CT.
 Dispensing module:
o Rocker: to support the percussion.

o Sampling probe: grooved on 75% of the length.
o Rinsing head: Reinforced and longer
o Probe belt: Larger
o Probe carriage: Larger
o Rocker motor: More powerful
o Start cycle: New design of the front panel
o Pump: Generate vacuum, clean and dry of the probe
 Electronic:
o CPU board: New functionalities
 Fluidic:
o In Line filter: To avoid rubber pieces inside the Mythic™22 fluidic.

o Silencer: To decrease the noise of the pump.
o Upper needle tubing
o Electrovalve n°3: Modified to allow modifying the fluidic.
o Electrovalve n°19: Added to manage the pump.
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Mythic™22 CT: Description of the piercing cycle
1. Percussion: During this phase, an air bubble is generated inside the

probe. This bubble avoids liquid goes inside the probe during the
percussion. It is removed during the cleaning of the probe before step
2. At this stage, the pump is working (Aspiration and cleaning probe
inside and outside)
2. Sample sucking up: 18 µl.
3. During the rinsing of the WBC bath, around 3 µl of sample are

removed. Only 15 µl (as Mythic™22 OT) are used for the first dilution.
Note: Each time the probe goes in upper position, the probe is
cleaned and dried.
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Mythic™22 CT: Technician software
Check sensors menu:
These three sensors give the type of tube,

depending of the tube holder inside the
machine.
By default, the sensors positions should be:
Tube-1: 0
Tube-2: 0
Tube-3: 1
 Holder opened/closed: The detection is done by magnetic contact.
 If the holder is dismantled, after reassembly, the next adjustment

should be done:
o Service/Technician/Close tube adjust:
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 Adjust rocker: Adjust the centering

of the probe on the tube.
 Adjust holder: Adjust the holder

position.
In both cases, follow the instructions display by the menu.
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Mythic™22 CT: Recommendations for dismantling
 To dismantle the front panel, open the door to the half position.
 To dismantle the probe, move the rinsing head first, and then move the
probe from the probe carriage.
 If you change the needle or move it, it is mandatory to change the 0-
ring.
Troubleshooting :
If the O-ring is damaged, the following troubles could be

noted:
Trace of diluent on the head of the O-ring holder.

Trace of crystallization of diluent on the head of the O-ring
holder.
Risk of clog with rubber pieces.
Default of drying.
CHANGE OF NEEDLE = CHANGE OF O-RING
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Adjustment of the cap piercing module:

On the upper position of the module, a slot screw allows to adjust the
transverse position of the probe from the tube.
The pump is located under the reagent tray. This pump is protected with a
foam.
DO NOT MOVE THE INSTRUMENT IF IT IS NOT DRAINED OF ALL

THE LIQUIDS; REAGENTS LEAK ON THE BOTTOM PLATE COULD
SOAK THE FOAM AND WILL DAMAGE THE PUMP.
Reassembly of the holder:
1. Put the holder on the position; place the screws while pushing the
holder
in the top.
2. Place the front panel while pushing it in the bottom position.
3. Check that the door is correctly adjusted. If so, go to point 4, if not, go

to point 1.
4. Remove the front panel.
5. Adjust the transverse with the slot screw.
6. Tighten the four screws.
7. Put the front panel.
03/09/2009 - 136 - Orphée S.A/M22/SEW/002

Mythic™22 CT - Maintenance:
 Every three months (user):
o Greasing
o Cleaning of the in-line filter
o Check of the silencer (trace of humidity)
 Every six months (field service engineer):
o Greasing
o Change of probe O-ring
o Change of the in-line filter
03/09/2009 - 137 - Orphée S.A/M22/SEW/002

Mythic™22 CT: Fluidic scheme
03/09/2009 - 138 - Orphée S.A/M22/SEW/001

03/09/2009 - 139 - Orphée S.A/M22/SEW/001

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Mythic™22

Service Engineer Training

03/09/2009 -1- Orphée S.A/M22/SEW/003

Service engineer training workbook usage disclaimer

Information contained in this document was developed for use by trained

Mythic is a trademark of Orphée S.A.

03/09/2009 -2- Orphée S.A/M22/SEW/003

SEW/001 12/2006 First release

SEW/002 01/2008 Second release:

SEW/003 09/2009 Third release:

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Section 1 will provide and overview of the system and physical

After reviewing Section 1 of this workbook you should be able to do

 Identify the connections located on the rear of the

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Start Cycle key

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Note: For complete listing of physical specifications, refer to

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 List the main menus of the instrument.

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 None : User – Lab tech

Note1: with MAPD password, everything is possible, no alert

Choose the selected language with the scroll bar.

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1. Plug the USB key on

2. Go to Set Up, Advanced menu, Storage Options.

3. Then, press USB thumb drive format.

4. When the format is finished a prompt will appear.

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The serial number of the Mythic 22 is coded as : xxxxxx-yyyyyy

Common folders. The content of this part

All of these folders and datas contained in

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COMMONS DATAS : \ROOT\

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PRIVATES DATAS : \ROOT\xxxxxx\yyyyyy\

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5. The USB key is not connected on the instrument:

6. The USB key is connected on the instrument:

NOTE: To avoid the problem of memory full, we recommend activating the

How to install this driver:

 Plug your USB key on a computer.

 From the page

 Automatic Print-out Analysis:

The screen is shared in two parts:

By default, the current month is

- Host serial = RS232 connection

- Once you select one option, you can

- For the SERIAL PARAM., normally

- For the NET. Parameters, select

- The MAC address is defined on the

Toolbar available in each

Menu: Go back to Main

1. 2. Input lot, targets, limits or

1. Choose one lot 2. Input lot, targets and limits, or press