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DATO DE CALIBRADO BIOMASA

N° Dilución Absorbancia X(g/l)


1 5.0 0.815 0.400
2 6.0 0.713 0.333
3 8.0 0.559 0.250
4 10.0 0.451 0.200
5 15.0 0.249 0.133
6 20.0 0.128 0.100
7 25.0 0.093 0.080
8 30.0 0.08 0.067
9 50.0 0.45 0.040

Datos/dilucion 1 en 5 1 en 6 1 en 8 1 en 10 1 en 15
C1 2 2 2 2 2
V1 1 1 1 1 1
C2 X X X X X
V2 5 6 8 10 15

BLANCO [CÉLULA]
DILUCION Abs. Prom Capachito Peso (inicial)
1.4 0.944 1 0.2203
1.4 0.948 2 0.2354
1.4 0.970 3 0.2458
PROMEDIO 0.954 PROMEDIO 0.2338333333
1 en 20 1 en 25 1 en 30 1 en 50
2 2 2 2
1 1 1 1
X X X X
20 25 30 50

[CÉLULA]
Peso (final) Biomasa
0.2216 0.0013
0.2377 0.0023
0.2481 0.0023
0.2358 0.00196667
[Biomasa 2
inicial] (g/L)

N° Dilución Absorbancia [Biomasa] (g/L)


1 5.0 0.815 0.00196667
2 6.0 0.713 0.0016388917
3 8.0 0.559 0.0012291688
4 10.0 0.451 0.000983335
5 15.0 0.249 0.0006555567
6 20.0 0.128 0.0004916675
7 25.0 0.093 0.000393334
8 30.0 0.08 0.0003277783
9 50.0 0.045 0.000196667
CURVA DE CALIBRADO DE BIOMASA
0.9
0.8 f(x) = 469.67764598x - 0.0632779169
R² = 0.9860891143
0.7
0.6
Axis Title

0.5
Col umn D
0.4 Linear (Col umn D)
0.3
0.2
0.1
0
0 0.0005 0.001 0.0015 0.002 0.0025
Axis Title
N° Tubo Vol. Sol. Std (ml) Vol. Agua (ml) {} Azucares reductores Absorbancia (540 nm)
1 0.500 0 2 1.044
2 0.400 0.100 1.6 0.821
3 0.300 0.200 1.2 0.616
4 0.200 0.300 0.8 0.405
5 0.100 0.400 0.4 0.2
6 0.50 0.450 0.2 0.076
7 0 0.500 0 0

Para la {} en azucares reductores se utilizo la siguiente formula

C1*V1 = C2*V2

Datos TUBO 1 TUBO 2 TUBO 3 TUBO 5


C1 2 2 2 2
V1 0.500 0.400 0.300 0.100
C2 X X X X
V2 0.500 0.500 0.500 0.500
CURVA DE CALIBRADO DE AZUCARES REDUCTORES
1.2

1 f(x) = 0.5254095563x - 0.0136484642


R² = 0.9994346856
0.8
Absorvancia

0.6 Col umn F


Li near (Col umn F)
0.4

0.2

0
0 0.5 1 1.5 2 2.5

TUBO 7 Azucares reductores

2
0
X
0.5
N° Tiempo (h) HORA Absorbancia Dilución X (g/L) lnX
0 0 8:05 0.299 1 0.00077138 -7.1673342928
1 2 10:05 0.343 1 0.00086506 -7.0527150877
2 4 12:05 0.382 1 0.00094809 -6.9610586784
3 6 14:05 0.51 1 0.00122062 -6.7083977508
4 7 15:05 0.622 1 0.00145908 -6.5299501921
5 8 16:05 0.756 1 0.00174438 -6.3513565736
6 10 18:05 0.393 4 0.00388605 -5.5503620417
7 11 19:05 0.545 4 0.00518055 -5.2628443483
8 13 21:05 0.686 4 0.00638137 -5.0543730922
9 15 23:05 0.77 4 0.00709675 -4.9481188082

Lineal
-4
5 6 7 8 9 10 11 12 13 14
-4.5

-5 f(x) = 0.2604782731x - 8.2897558813


R² = 0.9091400694 Col umn H
Ln x

-5.5 Linea r (Col umn H)

-6

-6.5

-7
Tiempo
Biomasa
y=469.68X - 0.0633

Chart Title
0.008

0.007

0.006

0.005

0.004

0.003

0.002

0.001

0
0 2 4 6 8 10 12 14 16

U máx 0.2605
t ferm (h) 8.5190613613
PRUEBA DEL DNS Curva de calibrado
N° Tiempo (h) ABS Dilución S(g/L) A= 0.5254X- 0.0136
0 0 0.638 2 2.48
1 2 0.758 2 2.94
2 4 0.669 2 2.60
3 6 0.764 2 2.96
4 7 0.739 2 2.86
5 8 0.759 2 2.94
6 10 0.705 2 2.74
7 11 0.610 2 2.37
8 13 0.58 2 2.26
9 15 0.529 2 2.07
urva de calibrado
= 0.5254X- 0.0136 Chart Title
3.50

3.00

2.50

2.00

1.50

1.00

0.50

0.00
0 2 4 6 8 10 12 14 16
N° Tiempo (h) X (g/L) S(g/L)
0 0 0.00077138 2.48
1 2 0.00086506 2.94
2 4 0.00094809 2.60
3 6 0.00122062 2.96
4 7 0.00145908 2.86
5 8 0.00174438 2.94
6 10 0.00388605 2.74
7 11 0.00518055 2.37
8 13 0.00638137 2.26
9 15 0.00709675 2.07

Variacion de X y S
3.50 0.008
3.00 0.007
2.50 0.006
0.005
X o S (g/L)

Axis Title
2.00
0.004
1.50
0.003
1.00 0.002
0.50 0.001
0.00 0
0 2 4 6 8 10 12 14 16
Tiempo

S(g/L) X(g/L)
Y 7.66111097
Qx máx 0.02428165

U máx 0.2605
t ferm (h) 8.51906136