RANGSITAN 65

Supakorn Porsche Suwanlertlum

Mr.Abel Cadias

English 10 / Section 1001

May 17, 2019

Liver Enzyme in each Temperature Condition

Some cells can produce a poisonous chemical. However, the toxic chemical can’t affect

human body because the cells have an enzyme. It uses to breakdown the toxic chemicals into

innocuous substances(Curtis, 2019). The enzyme is not altered by the reaction and in the human

body, people have hundreds of different enzymes in each of the cells. Each enzyme is liable for a

specific reaction that happens in the cell. (Luo, 2018). Catalase is the name of a catalytic enzyme

that speeds up the reaction which separates hydrogen peroxide, a toxic chemical into water and

oxygen, which is a harmless substance. The reaction of this enzyme is important to cells because

hydrogen peroxide is produced as a byproduct of many normal cellular reactions. Enzyme activity

also has effects, depending on the temperature; therefore, the reaction of the enzyme would

increase or decrease.

The purpose of this research report is to find out which condition is the best for the

reaction of the enzyme. This research report provides a result from the experiment and giving out

the knowledge about enzyme to the reader. The author uses three academic articles from

different sources to relate the result and interpretation of the experiment to other research studies.

⅗+4 = 7
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The result of this experiment is the rating of bubbles produced and the time lapsed by using

the pork liver because of the catalase found in liver cells. It is possible to use the dead cells for the

experiment because the enzymes remain intact and active for several weeks (Zentella, 2016).

The figure below shows the time lapsed and rating of bubbles. The first condition is normal

liver and H202. It can generate bubbles because normal liver contains catalase enzyme in it which

can break down the hydrogen peroxide into water and oxygen. Therefore, the bubble is being

generated because of the oxygen gas. This concludes that normal liver at room temperature still

has enzyme remained inside. The second condition is heated liver and H202. it doesn’t produce

any bubbles because the enzyme is a protein. Protein can be denaturalized by the process of heating

(Luo, 2018). Therefore, heat destroys the enzymes contained inside of the liver. That is the reason

why there are no bubbles produced from the heated liver. The final condition is cold liver and

H202. It produces more bubbles than normal liver because of the cold temperature helps maintain

the enzyme inside the liver which means that the cold liver has more enzyme than the normal liver.

Figure 1 Rate of bubbles and

Time lapsed
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The cold liver and H202 is the best condition for the enzyme to effectively catalyze their

reaction because the cold temperature helps maintain the enzyme inside the liver. It helps to

catalyze more reaction; therefore, it can produce many bubbles. The time lapsed also produced in

a long time but it is still close to the time used in normal liver and H202 because of the low

temperature causes the molecular movement decreases and have a slow reaction (Luo, 2018). The

substrate is H202 and the product is water and oxygen gas (bubbles). Enzymes are reusable because

the enzyme itself is not consumed when the enzyme and substrate bind. It’s catabolic because it

releases energy, we can feel the heat (energy) that releasing by touching the test tube.

10/10

This research report concludes that the best condition is cold liver and H202. Some

conditions don’t generate bubbles because of the temperature that have an effect on the condition.

However, some conditions can produce the bubbles at a high rate and longer duration of reaction

depends on the temperature that happened with the conditions.

8/10

References

Contreras, Z. (2016). Is Liver Enzyme Release Really Associated with Cell Necrosis Induced by
Oxidant Stress? Retrieved April 24, 2019 from
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4699024/

Wong, K.(2019, May 14). Poison. Retrieved from April 24, 2019 from
https://www.britannica.com/science/poison-biochemistry

Newman, T. (2018, January 11). Enzymes: Function, definition, and examples.

Retrieved April 24, 2019 from
https://www.medicalnewstoday.com/articles/319704.php