1 COVERING LETTER

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3Dear Editor-in-Chief,
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5I herewith enclosed a research article,
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7Title:

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31

32 Title of the article should be written in compact, clear, and informative

33 sentence, preferably not more than 20 words (16pt)

34 AHMAD DWI SETYAWAN1,2,♥, YAYA I. ULUMUDDIN3, PANDISAMY RAGAVAN4,♥♥ (10 PT)

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35 Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University. Jl. Ir. Sutami 36A Surakarta 57 126, Central Java,
36 Indonesia. Tel./Fax. +62-271-663375, email: volatileoils@gmail.com. (Center, 8 pt)
2
37 Program of Conservation Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok 16424, West
38 Java, Indonesia.
3
39 Research Center for Oceanography, Indonesian Institute of Sciences (PPO-LIPI), East Ancol, North Jakarta 14430, Jakarta, Indonesia.
40 4
Institute of Forest Genetics and Tree Breeding, R.S.Puram, P.B. No 1061, Coimbatore 641002, Tamil Nadu, India. email: xxxxxx@yahoo.com.

41 Manuscript received: DD MM 2016 (Date of abstract/manuscript submission). Revision accepted: .................... 2016. (8 pt)

42Abstract. A concise abstract is required (± 200 words). The abstract should be informative and state briefly the aim of the research, the
43principal results and major conclusions. An abstract is often presented separately from the article, thus it must be able to stand alone
44(completely self-explanatory). References should not be cited, but if essential, then cite the author(s) and year(s). Abbreviations should
45be avoided, but if essential, they must be defined at their first mention. (9 pt)

46Key words: Keywords is about five words, covering scientific and local name (if any), research theme, and special methods which used;
47and sorted from A to Z. (9 pt)

48Abbreviations (if any): All important abbreviations must be defined at their first mention there. Ensure consistency of abbreviations
49throughout the article.

50Running title: a short title with five words

51 INTRODUCTION (10 PT)

52 Introduction is about 400-600 words, covering the aims of the research and provide an adequate background, avoiding
53a detailed literature survey or a summary of the results. Indicate the aims of research in the last paragraph. (10 pt)
54 Manuscript of original research should be written in no more than 8,000 words (including tables and picture), or
55proportional with articles in this publication number. Review articles will be accommodated, while, short communication
56should be written in about 2,000 words, except for pre-study.
57 Manuscript is typed on white paper of A4 (210x297 mm 2) size, in a single column single space, 10-point Times New
58Roman font, with margin text from the top is 3 cm, from the bottom is 2 cm, left and bottom are 1.8 cm. Smaller lettering
59size can be applied in presenting table and figure (9 pt). Word processing program or additional software can be used,
60however, it must be PC compatible and Microsoft Word based (.doc or .docx). Scientific names of species (incl.
61subspecies, variety, etc.) should be written in italic, except for italic sentence. Scientific name (genera, species, author), and
62cultivar or strain should be mentioned completely for the first time mentioning it in the body text, especially for taxonomic
63manuscripts. Name of genera can be shortened after first mentioning, except generating confusion. Name of the author can
64be eliminated after first mentioning. For example, Rhizopus oryzae L. UICC 524, hereinafter can be written as R. oryzae
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66nomenclature should follow the order of the IUPAC - IUB. For DNA sequence, it is better used Courier New font.
67Symbols of standard chemical and abbreviation of chemistry name can be applied for common and clear used, for
68example, completely written butilic hydroxytoluene (BHT) to be BHT hereinafter. Metric measurement use IS
69denomination, usage other system should follow the value of equivalent with the denomination of IS first mentioning.
70Abbreviations set of, like g, mg, mL, etc. do not follow by dot. Minus index (m -2, L-1, h-1) suggested to be used, except in
71things like “per-plant” or “per-plot”. Equation of mathematics does not always can be written down in one column with
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74decimal. In the text, it should be used “%” rather than “percent”. Avoid expressing ideas with complicated sentence and
75verbiage, and used efficient and effective sentence.

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 76 Citation in manuscript is written in “name and year” system; and is arranged from oldest to newest and from A to Z. In
77citing an article written by two authors, both of them should be mentioned, however, for three and more authors, only the
78last (family) name of the first author is mentioned, followed by et al. (not italic), for example: Saharjo and Nurhayati
79(2006) or (Boonkerd 2003a, b, c; Sugiyarto 2004; El-Bana and Nijs 2005; Balagadde et al. 2008; Webb et al. 2008). Extent
80citation as shown with word “cit” should be avoided. Reference to unpublished data and personal communication should
81not appear in the list but should be cited in the text only (e.g., Rifai MA 2007, pers. com. (personal communication);
82Setyawan AD 2007, unpublished data). A total of 80% of the references should be from scientific journals published in the
83last five years, except for taxonomic studies. Names of journals should be abbreviated according to the ISSN List of Title
84Word Abbreviations (www.issn.org/2-22661-LTWA-online.php).

85 MATERIALS AND METHODS (10 PT)

86Study area (10 pt)

87 Materials and Methods should emphasize on the procedures and data analysis. For field study, it is better if study site is
88included (Figure 1).
89
90
91

92
93
94Figure 1. Location of João Pinheiro Stream, subaffluent of the Tibagi River indicating the sampling sites of Micropterus salmoides:
95point 1 (24º16’58.96”S, 50º35’00.04”W), point 2 (24º17’04.49”S, 50º34’58.71”W); and the detected sites. ( Figure, 9 pt)
96

97Procedures
98Sub-procedures-1 (replace with your sub-sub-title of procedures)
99 Xxxxxx.

100Sub-procedures-2
101 Xxxxxx.

102Data analysis
103 Xxxxxx.

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104 RESULTS AND DISCUSSION

105 Results and Discussion should be written as a series of connecting sentences, however, for manuscript with long
106discussion should be divided into subtitles. Results should be clear and concise.

107Result-1 (replace with your sub-sub-title of result)

108 Figures and tables of maximum of three pages should be clearly presented. Number tables consecutively in accordance
109with their appearance in the text. Title of a picture is written down below the picture, while title of a table is written above
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111preferred to use black and white images. Author could consign any picture or photo for the front cover, although it does not
112print in the manuscript. All images property of others should be mentioned source. There is no appendix, all data or data
113analysis are incorporated into Results and Discussions. For broad data, it can be displayed on the website as a supplement
114(Figure 2; Table 1).

115Result-2
116 Xxxxxxxxx (Table 2).
117 Xxxxxxxxx (Figure 3).
118
119Table 1. Diagnostic character of S. caseolaris, S. lanceolata, S. x urama and S. x gulngai with references to Duke and Jackes (1987) and
120Duke (2006). (9 pt)
121
S. lanceolata S. x urama S. x gulngai
Characters Duke and Jackes Duke and Present study
Present study Duke 2006 Present study
(1987) Jackes (1987)
Leaf shape
Leaf apex
Leaf base

122Note: Xxxxxx
123

124Discussion
125 Thorough discussion represents the causal effect mainly explains for why and how the results of the research were
126taken place, and do not only re-express the mentioned results in the form of sentences , not repeat them. Concluding
127sentence should be given at the end of the discussion.

128 ACKNOWLEDGEMENTS

129 Acknowledgments are expressed in a brief; all sources of institutional, private and corporate financial support for the
130work must be fully acknowledged, and any potential conflicts of interest are noted.

131 REFERENCES

132Journal:
133Saharjo BH, Nurhayati AD. 2006. Domination and composition structure change at hemic peat natural regeneration following burning; a case study in
134 Pelalawan, Riau Province. Biodiversitas 7: 154-158.
135Book:
136Rai MK, Carpinella C. 2006. Naturally Occurring Bioactive Compounds. Elsevier, Amsterdam.
137Chapter in book:
138Webb CO, Cannon CH, Davies SJ. 2008. Ecological organization, biogeography, and the phylogenetic structure of rainforest tree communities. In:
139 Carson W, Schnitzer S (eds) Tropical Forest Community Ecology. Wiley-Blackwell, New York.
140Abstract:
141Assaeed AM. 2007. Seed production and dispersal of Rhazya stricta. 50th annual symposium of the International Association for Vegetation Science,
142 Swansea, UK, 23-27 July 2007.
143Proceeding:
144Alikodra HS. 2000. Biodiversity for development of local autonomous government. In: Setyawan AD, Sutarno (eds) Toward Mount Lawu National Park;
145 Proceeding of National Seminary and Workshop on Biodiversity Conservation to Protect and Save Germplasm in Java Island. Sebelas Maret
146 University, Surakarta, 17-20 July 2000. [Indonesian]
147Thesis, Dissertation:
148Sugiyarto. 2004. Soil Macro-invertebrates Diversity and Inter-Cropping Plants Productivity in Agroforestry System based on Sengon. [Dissertation].
149 Brawijaya University, Malang. [Indonesian]
150Information from internet:

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151Balagadde FK, Song H, Ozaki J, Collins CH, Barnet M, Arnold FH, Quake SR, You L. 2008. A synthetic Escherichia coli predator-prey ecosystem. Mol
152 Syst Biol 4: 187. www.molecularsystemsbiology.com

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153 SUBMISSION CHECKLIST
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156Ensure that the following items are present:
157
The first corresponding author must be accompanied with contact details: Give mark (X)
 E-mail address
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country)
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All necessary files have been uploaded, and contain:

 Keywords
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Further considerations
 Manuscript has been “spell & grammar-checked” Better, if it is revised by a professional
science editor or a native English speaker
 References are in the correct format for this journal
 All references mentioned in the Reference list are cited in the text, and vice versa
 Colored figures are only used if the information in the text may be losing without those
images
 Charts (graphs and diagrams) are drawn in black and white images; use shading to
differentiate
158
159