Code 9A23304 1

II B.Tech I semester (R09) Regular Examinations, November 2010

MICROBIOLOGY
(Biotechnology)
Time: 3 hours Max Marks: 70
Answer any FIVE questions
All questions carry equal marks
?????

1. (a) Write a detailed account of major events in the field of microbiology.

(b) Write a typical characteristics features of bacteria and algae.

2. (a) Write characteristics of bacteria with special reference to Archaea and Eubacteria.
(b) Describe briefly about different classification systems in microbiology.

3. (a) Describe macro and micronutrients required by microorganisms for their growth.
(b) Describe different pure culture methods spread plate , pour plate and streak plate methods.

4. (a) Write about different methods for preservation of microorganisms.

(b) Write about bacterial staining techniques.

5. (a) Write about definition of infection, diseases and pathogens with examples.
(b) Write about virulence and virulence factors of influenza and tuberculosis.

6. (a) Write 3 examples of animal viruses and plant viruses.

(b) Describe the classification of viruses.

7. (a) Describe the bacterial replication and also mechanism of lysogeny.

(b) Write about DNA viruses and RNA viruses.

8. (a) Write a detailed account on different method of identification and invitro cultivation of
viruses.
(b) Write about assay of animal viruses.

?????
Code 9A23304 2
II B.Tech I semester (R09) Regular Examinations, November 2010
MICROBIOLOGY
(Biotechnology)
Time: 3 hours Max Marks: 70
Answer any FIVE questions
All questions carry equal marks
?????

1. (a) Explain the theory of spontaneous generation.

(b) Write an account of microbial diversity.

2. (a) Write two scientists name and their contribution in the field of microganisms.
(b) Write about major characteristics used in taxonomy of microganisms.

3. (a) Write about microganisms classification based on nutrition.

(b) What are the special techniques used for cultivation of anaerobes.

4. (a) Write about working and primary stock cultures-Lyophilisation.

(b) Write about influence of environmental factors on the growth microganisms.

5. (a) Write about definition of infection diseases and pathogens with examples.
(b) Write about virulance and virulance factors of influenza and tuberculosis.

6. (a) Write about the properties of viruses and illustrate the virus structure.
(b) Write about the application of viruses in biotechnology industry.

7. (a) Describe the bacterial virus replication and also mechanism of lysogeny.
(b) Write about DNA viruses and RNA viruses

8. (a) Write a detailed account on different methods of identification and invitro cultivation of
viruses.
(b) Write about assay of animal viruses.

?????
Code 9A23304 3
II B.Tech I semester (R09) Regular Examinations, November 2010
MICROBIOLOGY
(Biotechnology)
Time: 3 hours Max Marks: 70
Answer any FIVE questions
All questions carry equal marks
?????

1. (a) Write about germ theory of diseases

(b) Write different techniques to identify micro organsims.

2. (a) What is the significance of microbial classification? Describe the classification of Carl-
Wooese et al.
(b) Describe different taxonomic ranks in microbiology with special reference to bacteria.

3. (a) Write briefly on nutritional classes of microorganisms.

(b) Write a detailed account on different types of media- selective media, differential media
and enrichment media.

4. (a) Write about use of agar glants, agar stabs for preservation of microorganisms.
(b) Write about aims and principles of biochemical test - sugar fermentations IMVIC tests
and catalase production.

5. (a) Write about definition of infection, diseases and pathogens with examples.
(b) Write about virulence and virulence factors of influenza and tuberculosis.

6. (a) Write 3 name of animal viruses and plant viruses.

(b) Describe the classification of viruses.

7. (a) Describe the bacterial replication and also mechanism of lysogeny.

(b) Write about DNA viruses and RNA viruses.

8. (a) Write a detailed account on different methods of identification and invitro cultivation of
viruses.
(b) Write about assay of animal viruses.

?????
Code 9A23304 4
II B.Tech I semester (R09) Regular Examinations, November 2010
MICROBIOLOGY
(Biotechnology)
Time: 3 hours Max Marks: 70
Answer any FIVE questions
All questions carry equal marks
?????

1. (a) Write two scientists names and their contribution in the field of microbiology.
(b) Write typical characteristics features of fungi an their significance role in medicine.

2. (a) What are the different growth factors and their functions in microbial metabolism?
(b) Describe cultivation of microorganisms and also describe the role of solidifying agents in
media.

3. (a) Write about preservation of microorganisms-use of sterile oil and cryopreservation .

(b) Write a detailed account on simple staining differential staining and endospore staining.

4. (a) Write about three domains of life.

(b) Write briefly about biochemical, immunological and genetical characters referring to Tax-
onomy of microorganisms.

5. (a) Write about definition of infection, diseases and pathogens with examples.
(b) Write about virulance and virulance factors of influenza and tuberculosis.

6. (a) Write about the properties of viruses and illustrate the virus structure.
(b) Write about the application of viruses in biotechnology industry.

7. (a) Describe the bacterial replication and also mechanism of lysogeny.

(b) Write about DNA viruses and RNA viruses.

8. (a) Write a detailed account on different methods of identification of viruses and in vitro
cultivation of viruses.
(b) Write about assay of animal viruses.

?????