HIKMAH MODULE

SCIENCE SPM
MODULE 8
PAPER 1

1. The table below shows the temperature and pH some bacterial culture in a petri dish.

Table 1

Which petri dish was cloudier after two days?

A. P
B. Q
C. R
D. S

2. Active immunity is obtained when vaccine which injected into the blood stream

A. requires antibiotic for treatment

B. stimulates the body to produce white blood cell
C. stimulates the body cells to produces antibody
D. requires serum containing antibody.

3. Algae are neither parasite nor saprophyte because they are

A. Do not carry out respiration.

B. Carry out photosynthesis
C. Can live without doing any activity
D. Can synthesize organic substances

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4. The diagram below shows a type of a microorganism.

Figure 1

How does this microorganism reproduce?

A. releasing spores
B. binary fission
C. budding
D. bacteriophage

5. Which of the following statement is not true about viruses?

A. contain deoxyribonucleic acid
B. live as saprophyte
C. do not carry out respiration
D. the smallest microorganism

6. Which disease is spread by housefly?

A. Dengue
B. Cholera
C. Malaria
D. Hepatitis B

7. Which an effective method to prevent ringworm?

A. injection
B. sterilization
C. vector control
D. personal hygiene

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8. Penicillin is an

A. antigen
B. antiseptic
C. antitoxine
D. antibiotic

9. The microorganism which helps in the digestion of cellulose in herbivores is

A. algae
B. fungi
C. virus
D. bacteria

10. Yeast is added to pineapple juice in a conical flask. The mixture is left for five days.
The process that occurs is

A. fermentation
B. esterification
C. oxydation
D. distillation

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PAPER 2
SECTION A + B

1. Figure 1 shows an experiment carried out to study the effect of pH on the growth of
microorganisms.

Figure 1

Three sterile test-tubes labeled A, B and C with different pH are placed in the dark place
at room temperature for two days. The observations are after two days.

a. What is the hypothesis of this experiment?

b. Which test-tube is the cloudiest?

c. Why do we need to sterilise the test-tubes, cotton wool and nutrient agar before
the experiment?

d. State the manipulated variables of this experiment.

e. Base on this experiment, state the meaning of the microorganism

(operational definition)

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2. Figure 2 shows an experiment carried out to study the effect of temperature on the
growth of microorganisms.

Figure 2

Three sterile test-tubes A, B and C are placed at different location for two days. The
observation are made and recorded in Table 1

Test-tube Location Observation

A Refrigerator Still remains

B Dark place in laboratory

Cloudy
C Incubator Still remains

Table 1

a. What is the hypothesis of this experiment?

b. State the constant variable in this experiment.

c. What inference can you make based on this experiment?

d. Why do foods stored in refrigerator last longer?

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e. Does human body temperature suits the growth of bacteria?

3. Figure 3 shows the life cycle of the mosquito.

Figure 3

a. Name stage Q.

b. Why is the mosquitoes called vector?

c. Name two diseases can be spread by mosquitoes

i.

ii.

d. Suggest one ways to control mosquitoes at stage P.

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4. Figure 5 shows the life cycle of a housefly

Figure 4

a. Name the stage P and Q

P:

Q:

b. How can houseflies be controlled at stage Q?

c. Name one disease can be spread by houseflies.

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5. Figure 5 shows an experiment to study the effect of an antibiotic on bacterial

growth.

Figure 5

Three antibiotic discs, A1, A2 and A3 with different concentrations are placed on
nutrient agar surface. The petri dish is incubated inverted at 37oC. After two days, a
clear area is formed around each disc. The diameter of the clear area is measured
and recorded in Table 2.

Antibiotic Disc Diameter of the clear area (cm)

A1 1.2
A2 0.6
A3 1.6

Table 2
a. State one hypothesis for this experiment.

b. From this experiment, state the:

i. manipulated variable

ii. responding variable

iii. fixed variable

c. Why the petri dish should incubate inverted?

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d. Based on this experiment defined antibiotic operationally.

e. Name one disease which can be treated by using antibiotic.

6. Figure 6 shows the level of antibody.

Figure 6

a. Name the type of immunity shows in the diagram above.

b. Give one example of disease which can be prevented using this immunity.

c. Why should we need a second dose?

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7. Table 3 shows the results of an experiment to study the effects of temperature on

bacterial growth.

Temperature/ 0C Size of bacteria colony after 24 hours

0 2
15 5
26 10
35 15
60 3

a. State one hypothesis that can be made from table 2

b.
i. Based on Table 3, draw graph to show the relationship between the size of
bacterial colony and temperature.

ii. Base on the graph, state the optimum temperature for bacterial growth.

iii. Predict the size colony at 80oC.

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SECTION C

1. Study statement below:

Light can effect the growth and reproduction of bacteria

a. Suggest one suitable hypothesis to investigate the above statement.

b. Explain an out line of the experiment to test the hypothesis base on the following
criteria:
i. Aim of experiment.
ii. Identifying variables
iii. List of material and apparatus
iv. Method
v. Tabulation of data

2. a.
Dengue fever is cause by a virus whereas tuberculosis is caused by bacteria

State four differences between viruses and bacteria.

b.
The residents of Kampong Kuala Paya were caught off guard by the news of
boy in their neighbourhood being effected by dengue fever. The residents
decided to overcome the problem.

Explain how the residents in the area overcome the problem caused by
mosquitoes. Your answer should include the following:
Identify the problem
Clarification the problem
Solving methods
Choose the best method and explain your choice.

3. Study the following statement.

Temperature influences the growth of microorganism.

You are given some sterile nutrient broth and bacterial culture.
a) Suggest a hypothesis to investigate the above statement.
b) Describe one experiment to test your hypothesis based on the following criteria.

i. aim of the experiment

ii. identifying the variables
iii. list of apparatus and material
iv. method
v. tabulation of data

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MODULE 8 - ANSWERS
PAPER 1
1. C 2. C 3. B 4. A 5. B 6. B 7. D 8. D 9. D 10. A

PAPER 2
SECTION A + B

1.
a. Neutral environment with pH is the most suitable for the growth of bacteria.
b. Test-tube A
c. to ensure bacteria from outside do not interfere with the result of the experiment.
d. pH value
e. Microorganism is shown by the cloudiest of nutrient agar.

2.
a. room temperature is the most suitable temperature for the growth of the
microorganism.
b. type of bacteria/volume of nutrient agar/pH
c. test-tube B is cloudy because the microorganism/bacteria is suitable for growth at
room temperature.
d. microorganism cannot grow at very low temperature.
e. yes, it does

3.
a. pupae
b. mosquitoes carried pathogen.
c. i. dengue fever ii. Malaria
d. spray insecticides.

4.
a. pupae, larvae
b. cover food properly ; clean the garbage and sewage
c. cholera

5.
a. antibiotic can kill bacteria
b. i. different concentrations of antibiotic.
ii. diameter of the clear area
iii. type of bacteria/ room temperature@ 370C
c. to prevent water vapour from interfere the result of the experiment.
d. Antibiotic is shown by the diameter of the clear area.
f. syphilis/gonorrhea.

6.
a. artificial active immunity
b. rubella/chickenpox….
c. as a booster dose to increase the antibody level .
d. substance(weakened/dead pathogens) that stimulate white blood cell to produce
antibodies.

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7.
a. the most suitable temperature for the growth of the microorganism/bacteria is 370C

b. i.

ii. 360C +_ 1
iii.0/zero/none

SECTION C

Criteria Explanation

i.Aim To study the effect of light to the growth and

reproduction of bacteria.
ii. Identifying variables i. manipulated variables: light conditions
ii. fix variables: same quantity of nutrient
agar/same type of bacteria/same
pH/temperature(room temperature).
iii. responds variables: the growth of
bacteria(the cloudiness of nutrient agar)

iii.List material and apparatus a. bacterial culture, nutrient agar

b. two test-tube, sterile cotton wool

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vi. method

a. two test-tube labeled A and Bas shown

above is prepared.
b. 5 cm3 of nutrient agar and 1 cm3 of
Bacillus Subtilis bacterial culture is put
into each test-tube.
c. each test-tube is covered with sterile
cotton wool.
d. test-tube A is placed in dark place and
test-tube B is placed near the window for
two days.
e. The observation is recorded. in the table
after two days.

v. Tabulation of data
Test-tube Observation

A
B

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SECTION C
1.
a. bacteria can growth and reproduce in dark place

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3.

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