Professional Documents
Culture Documents
Abdul Hafeez
Plant Pathologist
Department of Agriculture Azad Kashmir
microorganisms Environmental factors
WHAT IS
PLANT DISEASE ?
Series of responses
morphology
(shape)
abnormality
(function)
physio logy
Disease
quant ity
quality
loss
Symptoms
leaf spot
Secondary Symptoms
Indirect & subsequent changes of a distance
from the site of the pathogen
Sclerotium wilt
Localized symptoms
leaf spot
bacterial canker
leaf blight (death of cells: not restricted)
Rots
(Death of cells and tissues)
anthracnose
phyllody
Stunting/Dwarfing
(Reduced Growth)
Dwarf
mosaic
phosphorus deficiency
Symptoms with Signs
rust
Symptoms with
Signs
smut
Symptoms with Signs
powdery mildew
downy mildew
Naming of Disease
1. With symptom
papaya ringspot
Watermelon fruit rot
2. With sign
rust
smut
powdery mildew
Causal agents
1) Noninfectious/abiotic agents
- imbalance of nutrients
- extreme temperature/moisture
- Air & water pollutants, chemicals, etc.
bacteria
mollicutes
Parasitic
higher
plants
viruses viroids
nematodes
protozoa
Disease
Triangle
Ti
me
Host
Disease Cycle
(1)
Reproduction Dissemination
Polycyclic Disease
(5)
Symptom (2)
(4)
development inoculation
Infection
(3) Monocyclic Disease
Diagnostic Techniques
1. Visual inspection of symptoms
2. Microscopic examination
3. Isolation for pure culture of pathogen
and Identification of pure culture
4. Biochemical & physiological methods
5. Nucleic acid-based methods
(molecular methods)
Visual Inspection of
Symptoms
Determine : disease VS insect
insect
disease
insec
t
Visual Inspection of Symptoms
biotic abiotic
Visual Inspection of Symptoms
Determine : Nutrient Deficiency VS Virus
virus
nutrient deficiency
Visual Inspection of Symptoms
Fungal Diseases
Use hand-lens/microscope
- mycelium/fruiting body/
spores
Bacterial Diseases
cut stem
from xylem
bacterial ooze
virus
insect vector
phytoplama
Virus and Phytoplasma
• Transmission test
• Indicator plant
• Serology
• Electron microscope
• Nucleic Acid, DNA
Nematode Damage
Symptom, Population density in soil & root
poor growth
root knot
(gall)
Nematodes in
stained root
Principles of Plant Disease Control
A. Geographic Selection
B. Site Selection
C. Environmental Management
D. Cultural Practices
– Planting/Harvest dates
– Short season cultivars
– Cultivation practices, plant spacing
– Irrigation management, fertility management
Reducing Rate of Development
Protection
A. Biological control
– Antagonistic organisms (Streptomyces, Bacillus,
Trichoderma, Paecillomyces, Verticillium)
– Cross protection (inoculate mild strain to induce
resistance for virulent strain of pathogen)
B. Chemical control
– Protectant, Eradicant
– Contact, systemic pesticides
– Fungicides, bactericides, nematicides
Integrated Plant
Disease Management
1. Manage the pathogen
2. Manage the host
3. Manage the environment
Integrated Plant Disease
Management
- Water management